Enhanced human bone marrow stromal cell affinity for modified poly(L-lactide) surfaces by the upregulation of adhesion molecular genes
Data(s) |
01/12/2009
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Resumo |
To enhance and regulate cell affinity for poly (l-lactic acid) (PLLA) based materials, two hydrophilic ligands, poly (ethylene glycol) (PEG) and poly (l-lysine) (PLL), were used to develop triblock copolymers: methoxy-terminated poly (ethylene glycol)-block-poly (l-lactide)-block-poly (l-lysine) (MPEG-b-PLLA-b-PLL) in order to regulate protein absorption and cell adhesion. Bone marrow stromal cells (BMSCs) were cultured on different composition of MPEG-b-PLLA-b-PLL copolymer films to determine the effect of modified polymer surfaces on BMSC attachment. To understand the molecular mechanism governing the initial cell adhesion on difference polymer surfaces, the mRNA expression of 84 human extracellular matrix (ECM) and adhesion molecules was analysed using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). It was found that down regulation of adhesion molecules was responsible for the impaired BMSC attachment on PLLA surface. MPEG-b-PLLA-b-PLL copolymer films improved significantly the cell adhesion and cytoskeleton expression by upregulation of relevant molecule genes significantly. Six adhesion genes (CDH1, ITGL, NCAM1, SGCE, COL16A1, and LAMA3) were most significantly influenced by the modified PLLA surfaces. In summary, polymer surfaces altered adhesion molecule gene expression of BMSCs, which consequently regulated cell initial attachment on modified PLLA surfaces. |
Formato |
application/pdf |
Identificador | |
Publicador |
Elsevier |
Relação |
http://eprints.qut.edu.au/28257/1/c28257.pdf DOI:10.1016/j.biomaterials.2009.09.013 Mao, Xueli, Peng , Hui, Ling, Junqi, Friis, Thor, Whittaker, Andrew K., Crawford, Ross W., & Xiao, Yin (2009) Enhanced human bone marrow stromal cell affinity for modified poly(L-lactide) surfaces by the upregulation of adhesion molecular genes. Biomaterials, 30(36), pp. 6903-6911. |
Direitos |
Copyright © 2009 Elsevier Ltd. All rights reserved |
Fonte |
Faculty of Built Environment and Engineering; Institute of Health and Biomedical Innovation; School of Engineering Systems |
Palavras-Chave | #110800 MEDICAL MICROBIOLOGY #069900 OTHER BIOLOGICAL SCIENCES #bone marrow #stem cell #cell adhesion #polylactic acid #adhesion mechanism #gene expression |
Tipo |
Journal Article |