The fearsome island, being a modern rendering of the narrative ofone Seld Fordred, master marines of Hythe, whose shipwreck and subsequent adventures are herein set forth...

UANL

Selenocysteine incorporation in Kinetoplastid: Selenophosphate synthetase (SELD) from Leishmania major and Trypanosoma brucei

Selenophosphate synthetase (EC 2.7.9.3), the product of the selD gene, produces the biologically selenium donor compound, monoselenophosphate, from ATP and selenide, for the synthesis of cysteine. The kinetoplastid Leishmania major and Trypanosoma brucei selD genes were cloned and the protein overexpressed and purified to apparent homogeneity. The selD gene in L. major and T brucei respectively 1197 and 1179 bp long encoding proteins of 399 and 393 amino acids with molecular of 42.7 and 43 kDa. The molecular mass of 100 kDa for both (L. major and T brucei) SEWS is consistent dimeric proteins. The kinetoplastid selD complement Escherichia call (WL400) selD deletion it is a functional enzyme and the specific activity of these enzymes was determined. A conserved residue was identified both by multiple sequence alignment as well as by functional and activity assay of the mutant (Cys to Ala) forms of the SELD identifying this residue as essential for catalytic function. (C) 2008 Elsevier B.V. All rights reserved.

Identification of a novel selD homolog from Eukaryotes, Bacteria, and Archaea: Is there an autoregulatory mechanism in selenocysteine metabolism?

Escherichia coli selenophosphate synthetase (SPS, the selD gene product) catalyzes the production of monoselenophosphate, the selenium donor compound required for synthesis of selenocysteine (Sec) and seleno-tRNAs. We report the molecular cloning of human and mouse homologs of the selD gene, designated Sps2, which contains an in-frame TGA codon at a site corresponding to the enzyme’s putative active site. These sequences allow the identification of selD gene homologs in the genomes of the bacterium Haemophilus influenzae and the archaeon Methanococcus jannaschii, which had been previously misinterpreted due to their in-frame TGA codon. Sps2 mRNA levels are elevated in organs previously implicated in the synthesis of selenoproteins and in active sites of blood cell development. In addition, we show that Sps2 mRNA is up-regulated upon activation of T lymphocytes and have mapped the Sps2 gene to mouse chromosome 7. Using the mouse gene isolated from the hematopoietic cell line FDCPmixA4, we devised a construct for protein expression that results in the insertion of a FLAG tag sequence at the N terminus of the SPS2 protein. This strategy allowed us to document the readthrough of the in-frame TGA codon and the incorporation of 75Se into SPS2. These results suggest the existence of an autoregulatory mechanism involving the incorporation of Sec into SPS2 that might be relevant to blood cell biology. This mechanism is likely to have been present in ancient life forms and conserved in a variety of living organisms from all domains of life.

Formation of a ternary complex for selenocysteine biosynthesis in bacteria

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

§ 105 der octroyirten Verfassung: Jedem verständigen Menschen geht das Gewisse über das Ungewisse ...

Welsch (Projektbearbeiter): Ablehnung des Artikels 105 der oktroyierten Verfassung, der in dringenden Fällen und bei Abwesenheit der beiden Kammern dem Ministerium legislative Befugnisse einräumt. Warnung vor den reaktionären Schriften, die in der Deckerschen Geheimen Ober-Hofbuchdruckerei erscheinen, als da sind: Neue Preußische Zeitung, Neues Preußisches Sonntagsblatt, das Volksblatt des Barons von Seld

Setzet den Fall, ein vornehmer und reicher Herr, mit dem Ihr einen Rechtsstreit gehabt ...

Welsch (Projektbearbeiter): Kritische Erörterung der oktroyierten Verfassung vom 5. Dezember 1848 in Bezug auf die Befugnisse des Minsteriums sowie auf die personelle Zusammensetzung der ersten Kammer. Das Ministerium kann Beschlüsse beider Kammern ablehnen, auch ohne die Deputierten Gesetze erlassen und im Falle eines Aufruhrs die demokratischen Grundrechte aufheben. Warnung vor der reaktionären Presse (Neue Preußische Zeitung, Neues Sonntagsblatt, Norddeutsche Zeitung, Volksblatt des Barons von Seld). Aufruf, nicht diejenigen Wahlmänner zu wählen, die von den Landräten, Beamten, Edelleuten und Pastoren anempfohlen werden

More odd texts of Chaucer's Minor poems /

"Date of issue, Mar. 1891."