Depth profiles of iodide and iodate during POLARSTERN cruise ANT-XXIV/3 to the Atlantic sector of the Southern Ocean

The biogeochemistry of iodine in the waters of the Atlantic sector of the Southern Ocean was investigated during the Polarstern cruise ANTXXIV-3 ZERO&DRAKE. The speciation and distribution of iodine (iodate and iodide) in seawater was examined across gradients of iron concentrations and phytoplankton abundance, ranging from an open ocean region along the Zero Meridian to the Weddell Sea and Drake Passage. Iodine cycling in high latitudes differs from that in low latitudes due to differences in the plankton community composition and the physicochemical characteristics. Iodate concentrations ranged between 400 and 450 nmol/L from the surface to the bottom. Surface concentrations of iodide (17 to over 60 nmol/L) were about an order of magnitude higher than below the pycnocline. The peak values of iodide lay nearly always within the euphotic zone and showed a weak, positive correlation with nitrite concentrations in the upper 200 m. In all vertical profiles a pronounced sub-surface maximum in iodide appears between 50 and 200 m depth indicating an iodide drawdown at the near surface. Iodide distribution in the Weddell Sea showed elevated levels in Weddell Sea Bottom Water (WSBW) indicating slow oxidation kinetics and the potential for iodide as a tracer of WSBW formation.

Chemical composition in phosphate materials from the ocean floor

Content, distribution patterns, and speciation of Cl in phosphorites and bone phosphate from the ocean floor, as well as in a set of samples from the land are studied. Total Cl content varies from 0.05 to 4.25% in phosphorites and from 2.48 to 2.75% in recent phosphate-bearing sediments. Recent phosphorites are enriched in Cl relative to ancient ones. Bound Cl content (not extractable by washing), which increases with lithification, varies from 0.17 to 0.60% in ocean and land phosphorites and from 0.02% to 1.30% in bone phosphate. Na content in most samples is higher relative to Na of NaCl due to its incorporation into the crystal lattice of apatite. However, the opposite relationship is observed in some samples indicating partial Cl incorporation into the anion complex of phosphate. Behavior of Cl in phosphorites from the present-day ocean floor is controlled by early diagenetic processes, whereas the role of weathering, catagenesis, and hydrogeological factors may be crucial for phosphorites on continents.

Composition of marine phosphorites and phosphate-bearing sediments

The book deals with behavior of phosphorus and its concentration in oceanic phosphorites. The major stages of marine geochemical cycle of phosphorus including its supply to sedimentary basins, precipitation from sea water, distribution and speciation in bottom sediments, diagenetic redistribution, and relation to other elements are under consideration. Formation of recent phosphorites as a culmination of phosphate accumulation in marine and oceanic sediments is examined. Distribution, structure, mineral and chemical compositions of major phosphorite deposits of various age on continental margins, as well as on submarine plateaus, uplifts and seamounts and some islands are described. A summary of trace element abundances in oceanic phosphorites is presented. Problems of phosphorite origin are discussed.

(Table 1) Elemental composition of benthic foraminifera from South Atlantic core top samples

The ocean plays a major role in the global carbon cycle, and attempts to reconstruct past changes in the marine carbonate system are increasing. The speciation of dissolved uranium is sensitive to variations in carbonate system parameters, and previous studies have shown that this is recorded in the uranium-to-calcium ratio (U/Ca) of the calcite shells of planktonic foraminifera. Here we test whether U/Ca ratios of deep-sea benthic foraminifera are equally suited as an indicator of the carbonate system. We compare U/Ca in two common benthic foraminifer species (Planulina wuellerstorfi and Cibicidoides mundulus) from South Atlantic core top samples with the calcite saturation state (Delta [CO3**2-] = [CO3**2-]in situ - [CO3**2-]sat) of the ambient seawater and find significant negative correlations for both species. Compared with planktonic foraminifera, the sensitivity of U/Ca in benthic foraminifera to changes in Delta [CO3**2-] is about 1 order of magnitude higher. Although Delta [CO3**2-] exerts the dominant control on the average foraminiferal U/Ca, the intertest and intratest variability indicates the presence of additional factors forcing U/Ca.

(Table 2) Contents of P2O5 and REE in Late Quaternary phosphorite nodules from Chile and Peru shelves

Changes in concentration levels and speciation of heavy metals during sedimentation on example of a typical semi-closed bay, where bottom sediments have formed due to river run-off, are under consideration. It is shown that due to desorption of mobile manganese, zinc and copper entered the bay with river suspended matter, their total contents in bottom sediments decrease and percentages of lithogenic forms increase. Contents and speciation of iron in bottom sediments are determined by its participation in coagulation of river colloids in the mixing zone and by mechanical differentiation of sedimentary material.

Differing responses of three Southern Ocean Emiliania huxleyi ecotypes to changing seawater carbonate chemistry

The invasion of anthropogenic carbon dioxide into the surface ocean is altering seawater carbonate speciation, a process commonly called ocean acidification. The high latitude waters of the Southern Ocean are one of the primary and most severely affected regions. Coccolithophores are an important phytoplankton group, responsible for the majority of pelagic calcium carbonate production in the world's oceans, with a distribution that ranges from tropical to polar waters. Emiliania huxleyi is numerically the most abundant coccolithophore species and appears in several different ecotypes. We tested the effects of ocean acidification on 3 carefully selected E. huxleyi ecotypes isolated from the Southern Ocean. Their responses were measured in terms of growth, photosynthesis, calcification, cellular geometry, and stoichiometry. The 3 ecotypes exhibited differing sensitivities in regards to seawater carbonate chemistry when cultured at the same temperature (14°C) and continuous light (110 µmol photons/m2/s). Under future ocean acidification scenarios, particulate inorganic to organic carbon ratios (PIC:POC) decreased by 38-44, 47-51 and 71-98% in morphotype A 'over-calcified' (A o/c), A and B/C, respectively. All ecotypes reduced their rate of calcification, but the cold-water adapted ecotype (morphotype B/C) was by far the most sensitive, and almost ceased calcification at partial pressure of carbon dioxide ( pCO2) levels above 1000 µatm. We recommend that future surveys for E. huxleyi cells in the Southern Ocean should include the capability of recognising 'naked cells' by molecular and microscopic tools. The distinct differences in the physiological responses of these 3 dominant Southern Ocean coccolithophore ecotypes are likely to have consequences for future coccolithophore community structures and thereby the Southern Ocean carbon cycle.

(Table 1) Contents of P2O5, organic carbon, and REE in recent diatomaceous ooze and phosphorites from the Namibian shelf

Changes in concentration levels and speciation of heavy metals during sedimentation on example of a typical semi-closed bay, where bottom sediments have formed due to river run-off, are under consideration. It is shown that due to desorption of mobile manganese, zinc and copper entered the bay with river suspended matter, their total contents in bottom sediments decrease and percentages of lithogenic forms increase. Contents and speciation of iron in bottom sediments are determined by its participation in coagulation of river colloids in the mixing zone and by mechanical differentiation of sedimentary material.

Acid-base regulation in the Dungeness crab (Metacarcinus magister)

Homeostatic regulation allows organisms to secure basic physiological processes in a varying environment. To counteract fluctuations in ambient carbonate system speciation due to elevated seawater pCO2 (hypercapnia), many aquatic crustaceans excrete/accumulate acid-base equivalents through their gills; however, not much is known about the role of ammonia in this response. The present study investigated the effects of hypercapnia on acid-base and ammonia regulation in the Dungeness crab, Metacarcinus magister on the whole animal and isolated gill levels. Hemolymph pCO2 and [HCO3]- increased in M. magister acclimated to elevated pCO2 (330 Pa), while pH remained stable. Additionally, hemolymph [Na+], [Ca2+], and [SO4]2- were significantly increased. When challenged with varying pH during gill perfusion, the pH of the artificial hemolymph remained relatively unchanged. Overall, ammonia production and excretion, as well as oxygen consumption, were reduced in crabs acclimated to elevated pCO2, demonstrating that either (amino acid) oxidation is reduced in response to this particular stress, or nitrogenous wastes are excreted in an alternative form.

Coccolithophore sensitivities to changing carbonate chemistry - an ecological framework

Coccolithophores are a group of unicellular phytoplankton species whose ability to calcify has a profound influence on biogeochemical element cycling. Calcification rates are controlled by a large variety of biotic and abiotic factors. Among these factors, carbonate chemistry has gained considerable attention during the last years as coccolithophores have been identified to be particularly sensitive to ocean acidification. Despite intense research in this area, a general concept harmonizing the numerous and sometimes (seemingly) contradictory responses of coccolithophores to changing carbonate chemistry is still lacking to date. Here, we present the "substrate-inhibitor concept" which describes the dependence of calcification rates on carbonate chemistry speciation. It is based on observations that calcification rate scales positively with bicarbonate (HCO3-), the primary substrate for calcification, and carbon dioxide (CO2), which can limit cell growth, whereas it is inhibited by protons (H+). This concept was implemented in a model equation, tested against experimental data, and then applied to understand and reconcile the diverging responses of coccolithophorid calcification rates to ocean acidification obtained in culture experiments. Furthermore, we (i) discuss how other important calcification-influencing factors (e.g. temperature and light) could be implemented in our concept and (ii) embed it in Hutchinson's niche theory, thereby providing a framework for how carbonate chemistry-induced changes in calcification rates could be linked with changing coccolithophore abundance in the oceans. Our results suggest that the projected increase of H+ in the near future (next couple of thousand years), paralleled by only a minor increase of inorganic carbon substrate, could impede calcification rates if coccolithophores are unable to fully adapt. However, if calcium carbonate (CaCO3) sediment dissolution and terrestrial weathering begin to increase the oceans' HCO3- and decrease its H+ concentrations in the far future (10 -100 kyears), coccolithophores could find themselves in carbonate chemistry conditions which may be more favorable for calcification than they were before the Anthropocene.

Urban forest health survey on Roznik in Ljubljana in 2013 by two methods

Urban forest health was surveyed on Roznik in Ljubljana (46.05141 N, 14.47797 E) in 2013 by two methods: ICP Forests and UFMO. ICP Forests is most commonly used monitoring programme in Europe - the International Co-operative Programme on the Assessment and Monitoring of Air Pollution Effects on Forests, which is based on systematic grid. UFMO method - Urban Forests Management Oriented method was developed in the frame of EMoNFUr Project - Establishing a monitoring network to assess lowland forest and urban plantations in Lombardy and urban forest in Slovenia (LIFE10 ENV/IT/000399). UFMO is based on non-linear transects (GPS tracks). ICP forests monitoring plots were established in July 2013 in the urban forest Roznik in Ljubljana .The 32 plots are located on sampling grid 500 × 500 m. The grid was down-scaled from the National Forest Monitoring survey, which bases on national sample grid 4 × 4 km. With the ICP forests method the following parameters for each tree within the 15 plots were gathered according to the ICP forests manual for Visual assessment of crown condition and damaging agents: tree species, percentage of defoliation, affected part of the tree, specification of affected part, location in crown, symptom, symptom specification, causal agents / factors, age of damage, damage extent, and damage extent on the trunk. With the UFMO method, the following parameters for each tree that needed sylviculture measure (felling, pruning, sanitary felling, thinning, etc.) were recorded: tree species, breast diameter, causal agent / damaging factor, GPS waypoint and GPS track. For overall picture in the urban forest health problems, also other biotic and abiotic damaging factors that did not require management action were recorded.