Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila.

We have used the green fluorescent protein (GFP) from the jellyfish Aequorea victoria as a vital marker/reporter in Drosophila melanogaster. Transgenic flies were generated in which GFP was expressed under the transcriptional control of the yeast upstream activating sequence that is recognized by GAL4. These flies were crossed to several GAL4 enhancer trap lines, and expression of GFP was monitored in a variety of tissues during development using confocal microscopy. Here, we show that GFP could be detected in freshly dissected ovaries, imaginal discs, and the larval nervous system without prior fixation or the addition of substrates or antibodies. We also show that expression of GFP could be monitored in intact living embryos and larvae and in cultured egg chambers, allowing us to visualize dynamic changes in gene expression during real time.

Analysis of perinatal gene expression: hormone response elements mediate activation of a lacZ reporter gene in liver of transgenic mice.

The transcription of genes encoding gluconeogenic enzymes is tightly regulated during the perinatal period. These genes are induced by glucagon (cAMP) and glucocorticoids and repressed by insulin. To address the role of cAMP and glucocorticoids in the physiological activation of genes encoding gluconeogenic enzymes in the perinatal period, transgenic mice have been generated with chimeric constructs containing the reporter gene lacZ under the control of hormone response elements. The activity of the transgene is restricted to the liver by the presence of the enhancers from the alpha-fetoprotein gene and its transcription is driven by a promoter that contains a TATA box linked to either cAMP response elements (CREs) or glucocorticoid response elements (GREs). We demonstrate cAMP and glucocorticoid regulation, liver-specific expression, and perinatal activation of the reporter gene. These data indicate that the CRE and GRE are, independently, necessary and sufficient to mediate perinatal gene activation. Perinatal activation was not impaired when a CRE reporter transgene was assayed in mice that contain a targeted mutation of the CRE-binding protein (CREB) gene, providing further evidence for functional redundancy among the members of the CREB/ATF gene family.

Micrornas 9a, 9b, 9c And 315 Regulate Expression Of A Reporter For The Neuronal Microtubule-Associated Protein Futsch/map1b

Fragile X syndrome (FXS) is the most common form of inherited mental retardation in humans. FXS is caused by loss of the Fragile X Mental Retardation Protein (FMRP), an important regulator of neuronal mRNA translation. Patients with FXS display cognitive deficits including memory problems. Protein synthesis-dependent long-term changes in synaptic plasticity are involved in the establishment and maintenance of long-term memory. One prevalent theory of FXS pathology predicts that FMRP is required to negatively regulate the translation of important mRNAs at the synapse. We are investigating microRNAs (miRNAs) as a potential regulator of synaptic FMRP-regulated mRNAs that have previously been described as being crucial to the process of synaptic plasticity. The general hypothesis underlying this thesis is that FMRP may negatively regulate the expression of futsch (the Drosophila homologue of the microtubule-associated protein gene MAP1B) via the miRNA pathway. The first step we took in testing this hypothesis was to confirm that futsch is subject to miRNA-mediated translational control. Using in silico target analysis, we predicted that several neuronally expressed miRNAs target the futsch mRNA 3'UTR and repress expression of Futsch protein. Then, using an in vitro luciferase reporter system, we showed that miR-315 and members of the miR-9 family selectively down-regulated futsch reporter translation. We have confirmed by site- directed mutagenesis that the miRNA interaction with the futsch 3'UTR is specific to the miRNA seed region binding site. Interestingly, reduction of FMRP levels by RNAi had no effect on futsch 3'UTR reporter expression. Together, these data suggest regulation of futsch expression by the miRNA pathway might be independent of FMRP activity. However, additional experiments need to be completed to confirm these preliminary results.

The southern reporter.

Vols. 6-142: Permanent ed.

Crop reporter.

Crop reporter.

Hearing; in the matter of the minimum wage recommendations of Special Industry Committee no. 17-D for the tobacco industry in Puerto Rico. September 6-8, 1955. Reporter: James A. Brownlee.

Mode of access: Internet.

The Western law reporter (Canada) and index-digest

Editors: 1905-Sept. 14, 1914, E.B. Brown; Sept. 21, 1914-May 1915, L.S. Le Vernois; June 1915-1916, E.K. Williams

The Ontario weekly reporter and index-digest

Publisher and sub-title vary: The Ontario weekly reporter and digest-index ... Toronto, Law books limited, 1916-17

The Code reporter.

Vols. 1-2 have subtitle : A journal for the judge, the lawyer, and the legislator, containing reports of practice cases, articles on legal topics, new rules of court...; v. 3, A monthly law journal, containing reports of decisions on the Code, articles on legal topics...

The Northwestern reporter.

Subtitle varies: v. 1-42, containing all the decisions of the Supreme courts of Minnesota, Wisconsin, Iowa, Michigan, Nebraska and Dakota ; v.43-243 comprising all the current decisions of the Supreme courts of Michigan, Iowa, Wisconsin, Nebraska, North Dakota and South Dakota with key number annotations (varies slightly); v. 244-300, Cases argued and determined in the courts of Iowa, Michigan, Minnesota, Nebraska, North Dakota, South Dakota, Wisconsin (varies slightly).

Transcript : Regional Public Meetings on the Resource conservation and recovery act of 1976, February 28 and March 1, 1977, Pittsburgh, Pa., [proceedings] ... transcribed by the official reporter, with handwritten corrections by the Office of Solid Waste.

Mode of access: Internet.

Transcript : Regional Public Meetings on the Resource conservation and recovery act of 1976, February 15 and 16, 1977, Kansas City, Mo., [proceedings] ... transcribed by the official reporter, with handwritten corrections by the Office of Solid Waste.

Mode of access: Internet.

America's textile reporter : for the combined textile industries.

"The most powerful influence in the textile industry."

Northeastern forest pest reporter.

Mode of access: Internet.