Diagn��stico de falha de transfer��ncia de imunidade passiva em bezerros atrav��s da determina����o de prote��na total e de suas fra����es eletrofor��ticas, imunoglobulinas g e m e da atividade da gama glutamil transferase no soro sang����neo

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Mandioca minimamente processada submetida a radia����o gama

Fresh cut cassava is an alternative to prolong roots commercialization period, adding value to the raw material and assisting the consumer's needs that seeks for products of easy preparation. The aim of this work was to evaluate the quality of fresh cut cassava subjected to gamma radiation. For processing cassava roots (IAC - 567-70) were used, after preparation were packed in expanded polystyrene (EPS) trays, covered by low density polyethylene (LDPE) plastic film, being subjected to gamma radiation in the doses 0 (control), 0.5; 1.0; 1.5; 2.0 KGy. Another treatment was also carried out in which roots were vacuum packed in nylon + polyethylene. Packed roots of all treatments were stored under refrigeration (5 +/- 1 degrees C) and evaluations were made after 0, 3, 6, 9 and 12 days. Evaluated analyses were: titrable acidity, pH, firmness, coloration, polyphenoloxidase activity, cooking time and roots hydration percentage. Evaluations just proceeded until the nine storage day, because after this period the roots already presented visually detectable physiologic and microbiological alterations. For titrable acidity, the vacuum packed roots had the lowest values, followed by the control packed in trays. For the other treatments, values of acidity increased and influenced the pH values. For the other appraised parameters there was no significant difference among treatments, but alterations were observed with the storage. The firmness decreased with the storage. Brightness values and yellow color component decreased with the storage and the b * (green) color values increased. Polyphenoloxidase activity was maximum in the day of elaboration of the product and it decreased with the storage. Cooking time and roots hydration percentage decreased with the roots storage.

Low-Resolution Molecular Models Reveal the Oligomeric State of the PPAR and the Conformational Organization of Its Domains in Solution

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Medium Chain Fatty Acids Are Selective Peroxisome Proliferator Activated Receptor (PPAR) gamma Activators and Pan-PPAR Partial Agonists

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)

Radia����o gama na conserva����o p��s-colheita da nectarina (Prunus persica var. Nucipersica) frigoconservada

O trabalho foi desenvolvido no laborat��rio de Frutas e Hortali��as do Departamento de Gest��o e Tecnologia Agroindustrial da Faculdade de Ci��ncias Agron��micas - UNESP - C��mpus de Botucatu, tendo como objetivo principal a caracteriza����o do comportamento da radia����o gama, na conserva����o p��s-colheita da nectarina cv. Sunred. Os frutos foram colhidos no in��cio do est��dio de matura����o, selecionados, limpos, pr��-resfriados (4ºC por 12 horas) e submetidos a diferentes doses de radia����o gama, constituindo assim os tratamentos: 1 0,0 kGy, 2 - 0,2 kGy, 3 - 0,4 kGy, 4 - 0,6 kGy, 5 - 0,8 kGy, sendo ap��s armazenados em c��mara fria com temperaturas de 0ºC e 90-95% de UR, por 28 dias. As an��lises foram realizadas a cada sete dias, determinando-se o aspecto visual dos frutos, a perda de massa fresca, a firmeza de polpa, a acidez total titul��vel (ATT), os s��lidos sol��veis totais (STT) e a raz��o STT/ATT. Ap��s 28 dias de armazenamento, verificou-se que os frutos submetidos �� dose de 0,4 kGy apresentaram o melhor aspecto visual, as menores perdas de massa fresca, e a maior firmeza de polpa, n��o ocorrendo, entretanto, varia����es significativas nos teores de acidez total titul��vel, s��lidos sol��veis totais e nos valores da raz��o SST/ATT.

Caracteriza����o e avalia����o de tr��s grupos de arroz-de-sequeiro de diferentes proced��ncias por meio da sensitividade �� radia����o gama

Coordena����o de Aperfei��oamento de Pessoal de N��vel Superior (CAPES)

Gama de hospedeiros e rea����o de gen��tipos de tomateiro a Pseudomonas cichorii

Em 2005, foi constatada em dois campos comerciais de tomate no Estado de S��o Paulo, a ocorr��ncia da queima bacteriana, causada por Pseudomonas cichorii. em vista disso, foram desenvolvidos estudos visando a determina����o da gama de hospedeiros de isolados de Pseudomonas cichorii (IBSBF 2309 e IBSBF 2323), obtidos de tomateiro, provenientes de campos comerciais localizados nos munic��pios de Bragan��a Paulista e Mogi Gua����, SP. Plantas de abobrinha, alface, beldroega, berinjela, beterraba, cenoura, couvebr��colo, datura, fumo, girassol, jil��, mel��o, pepino, pet��nia, piment��o, rabanete, repolho, r��cula, salsa e tomateiro foram inoculadas por pulveriza����o, separadamente, com os dois isolados de P. cichorii de tomateiro e um isolado de girassol (GIR-1). Os isolados IBSBF 2309 e IBSBF 2323 foram patog��nicos �� beldroega, datura, girassol, piment��o e tomate; GIR-1 foi patog��nico apenas �� beldroega, datura e girassol, n��o sendo patog��nico ao piment��o e ao tomateiro. No Brasil n��o se conhecem fontes de resist��ncia dentro do g��nero Lycopersicon ou a rea����o de cultivares de tomateiros a esta bact��ria. Vinte e oito gen��tipos de tomateiro provenientes do Banco de Germoplasma da empresa Sakata Seed Sudamerica Ltda., foram avaliados quanto a rea����o aos isolados IBSBF 2309 e IBSBF 2323 de P. cichorii, pelo m��todo de inocula����o nas folhas. Os maiores n��veis de resist��ncia foram observados em AF 11768, AF 2521, AF 11766, AF 11772, AF 229, AF 5719-1 e AF 8162. O gen��tipo AF 5719-1, que possui o gene Pto, que confere resist��ncia a P. syringae pv. tomato, apresentou um bom n��vel de resist��ncia a P. cichorii. A identifica����o de gen��tipos que apresentem bons n��veis de resist��ncia a este pat��geno �� importante para utiliza����o em programas de melhoramento gen��tico do tomateiro, visando a incorpora����o de genes de resist��ncia a P. cichorii.

Uso da sensitividade �� radia����o gama na discrimina����o de variedades de arroz-de-sequeiro dos grupos ��ndica e Jap��nica

Coordena����o de Aperfei��oamento de Pessoal de N��vel Superior (CAPES)

Efeitos de raios gama sobre trypanosoma cruzi. II. Anticorpos em camundongos inoculados com formas de cultura

Antibody fluorescent tests were carried out with serum and blood eluates from mice inoculated with different doses of irradiated (90 krad) and non irradiated T. cruzi culture forms. Non irradiated culture forms were more efficient immunogens than the irradiated ones. About 75% of the animals inoculated with non irradiated forms showed titers equal or higher than 1/16, the highest one reaching 1/64.

Uma revis��o sistem��tica sobre o uso de agonistas e antagonistas do GnRH em pacientes m��s respondedoras �� estimula����o ovariana

The purpose of this investigation was to make a systematic review of the medical literature in order to compare the efficacy of GnRH antagonists and agonists for poor responders to ovarian stimulation. According to the data collected, the use of GnRH antagonist protocols showed better results in comparison to long protocols with a GnRH agonist regarding the following aspects: lower cycle cancellation rate due to poor ovarian response; higher number of oocytes retrieved; higher clinical pregnancy rate per initiated cycle. Nevertheless, these results were not observed when the flare-up protocols of GnRH agonists were used. Moreover the number of oocytes retrieved with GnRH agonist was significantly higher in relation to the GnRH antagonist.

Uso da suplementa����o de LH recombinante na estimula����o ovariana com protocolos de FSH recombinante e agonistas do GnRH: Uma revis��o da literatura

The purpose of this review was to assess the efficacy of recombinant LH (r-LH) supplementation for controlled ovarian stimulation in recombinant FSH (r-FSH) and GnRH-agonist (GnRH-a) protocol for IVF/ICSI cycles. Search strategies included on-line surveys of databases from 1990 to 2006. Four trials fulfilled the inclusion criteria (Lisi et al. 2002, Humaidan et al. 2004, Marrs et al. 2004, Tarlatzis et al. 2006). When the review was carried out advantages were observed for the r-LH supplementation protocol with respect to a fewer days of stimulation, a fewer total amount of r-FSH administered and a higher serum estradiol levels on the day of hCG administration. However, these differences were not observed in number of oocyte retrieved, number of mature oocytes, clinical pregnancy per oocyte retrieval, implantation and miscarriage rates. Nevertheless, more randomized controlled trials are necessary before evidence-based recommendations regarding exogenous r-LH supplementation in ovarian stimulation protocols with r-FSH and GnRH-a for assisted reproduction treatment can be provided.

Chronic alcohol intake upregulates hepatic expression of carotenoid cleavage enzymes and PPAR in rats

Excessive and chronic alcohol intake leads to a lower hepatic vitamin A status by interfering with vitamin A metabolism. Dietary provitamin A carotenoids can be converted into vitamin A mainly by carotenoid 15,15���-monooxygenase 1 (CMO1) and, to a lesser degree, carotenoid 9���10���-monooxygenase 2 (CMO2). CMO1 has been shown to be regulated by several transcription factors, such as the PPAR, retinoid X receptor, and thyroid receptor (TR). The regulation of CMO2 has yet to be identified. The impact of chronic alcohol intake on hepatic expressions of CMO1 and CMO2 and their related transcription factors are unknown. In this study, Fischer 344 rats were pair-fed either a liquid ethanol Lieber-DeCarli diet (n = 10) or a control diet (n = 10) for 11 wk. Hepatic retinoid concentration and expressions of CMO1, CMO2, PPAR��, PPAR��, and TR�� as well as plasma thyroid hormones levels were analyzed. We observed that administering alcohol decreased hepatic retinoid levels but increased mRNA concentrations of CMO1, CMO2, PPAR��, PPAR��, and TR�� and upregulated protein levels of CMO2, PPAR��, and PPAR��. There was a positive correlation of PPAR�� with CMO1(r = 0.89; P<0.0001) and both PPAR�� and PPAR�� with CMO2 (r = 0.72, P< 0.001 and r = 0.62, P< 0.01, respectively). Plasma thyroid hormone concentrations did not differ between the control rats and alcohol-fed rats. This study suggests that chronic alcohol intake significantly upregulates hepatic expression of CMO1 and, to a much lesser extent, CMO2. This process may be due to alcohol-induced PPAR�� expression and lower vitamin A status in the liver. �� 2010 American Society for Nutrition.

Agonistas do GnRH, antagonistas do GnRH e a reprodu����o assistida: An��logos do GnRH x fertiliza����o in vitro

The agonists of gonadotropin-releasing hormone (GnRH) were introduced in ovarian stimulation for in vitro fertilization to avoid a premature surge of luteinizing hormone. Although they are accompanied by some disadvantages, GnRH agonists have become well accepted in clinical practice, and their use is associated with increased rates of pregnancy. The development of GnRH antagonists capable of blocking the pituitary immediately offered a therapeutic option. Comparative studies between the two analogs have suggested that the use of antagonists is associated with a shorter duration of ovulatory stimulus and a decreased incidence of ovarian hyperstimulation syndrome, while the type of GnRH analogues used show no significant effects on the rates of pregnancy and live birth. However, GnRH agonists have other applications in assisted reproductive technology cycles than the pituitary downregulation.

An��lise dos par��metros cl��nicos periodontais e express��o gen��tica de interferons alfa, gama e genes relacionados em indiv��duos portadores de S��ndrome de Down com doen��a periodontal

P��s-gradua����o em Ci��ncias Odontol��gicas - FOAR