Mutagênese induzida por flavonóides presentes do decocto das cascas da aroeira (Schinus terebinthifolius, Raddi)

The decoction of Brazilian pepper tree barks (Schinus terebinthifolius, Raddi), is used in medicine as wound healing and antiinflamatory. Once extracts from this plant are used for acceleration of scar s process, it is important to study their mutagenic and genotoxic potential. In previous works in our laboratory, it was observed mutagenicity caused by the decoction when in high concentrations. Among the chemical compounds of this plant that could be able to induce mutation, the flavonoids were the only group that was referred to have either an oxidant or antioxidant potential. The flavonoids were isolated, purified and quantified by adsorptive column chromatography under silica gel, bacterial and in vitro genotoxic tests were realized to determine if the flavonoids were the responsible agents for this mutagenicity found. The tests realized with plasmidial DNA were indicative that the flavonoids are probably genotoxic, due to the presence of correlation between increase of the flavonoid concentration and in plasmidial DNA double strand breakage visualized in agarose gel, as well as they were capable to generated abasic sites shown by the in vitro treatment with exonuclease III. The same tests with plasmidial DNA in the presence of copper [10 µM] and of a Tris-HCl pH 7.5 [10 µM] buffer were realized with the isolated flavonoids to determine if there would be or not participation of reactive oxygen species (ROS). The transformation of plasmidial DNA in different bacterial strains proficient and deficient in DNA repair enzymes in the presence or not of a Tris-HCl buffer, suggests that the enzymes that repair oxidative lesions are necessary to repair the lesions generated by the flavonoids and that ROS are generated and are necessary to promote the lesions. Bacterial tests with Escherichia coli strains of the CC collection (deficient or not for DNA repair enzymes), showed that the flavonoids are able to increase the frequency of mutations, mainly in strains mutated in repair enzymes (MutM, MutY-glicosylases and double mutant), suggesting that these agents are responsible for the enhancement in the mutation rate. In order to determine the mutation spectrum caused by the flavonoids of the Brazilian pepper tree stem bark, plasmidial DNA previously treated with the flavonoids were transformed in bacterial strains deficient and proficient in the DNA repair enzymes, followed by a blue-white selection with X-gal, DNA amplification by PCR and sequencing the positive mutant clones. Analysis of the mutants obtained from strains CC104, CC104mutM, CC104mutY, CC104mutMmutY, BW9101, BW9109 indicated a predominance of some mutations like G:C to C:G that can be correlated with the origin of 8-oxoG, due to oxidative lesions caused by the flavonoids. So it can concluded that the flavonoid isolated or in fractions enriched on them are genotoxic and mutagenic, and their mutations are predominantly oxidative, mediated by ROS, and the lesions are recognized by the BER system. In this way it is proposed that the flavonoids can act in two different ways to generate the DNA lesion: 1. in a Fenton-like reaction, when the flavonoid are in the presence of metal ions and that together with the water generate ROS that promotes the DNA lesions; 2. in another way the lesions can be generated by the formation of ROS due to the internal chemical structure of the flavonoid molecule due to the quantity and location of hydroxyl groups, and so producing the DNA lesions, those lesions can be directly (suggested by the in vitro experiments) or indirectly done (supported by the experiments using the CC bacterial strains)

Recorte dos produtos educacionais em história no ensino da matemática e em didática da matemática a partir das dissertações e teses defendidas no brasil entre 1990-2010

This work has the main purpose of conducting a survey of educational products present in dissertations and doctoral theses focused on the use of history in mathematics teaching and Didactics of mathematics with a French foundation produced in graduate programs in the strict sense of the Brazil between 1990 and 2010, the areas of Education, Mathematics Education, school of Natural Sciences and Mathematics and related areas, according to the research proposal of Mendes (2010). Our interest was to select the products that present concrete proposals for educational activities that can be used in the classroom of Basic Education and Training of Teachers of Mathematics. The research was implemented through a bibliographic study documents the Bank of dissertations and theses from CAPES, libraries and archives of some Postgraduate programs in the country who focus their studies on the subject object of this research, besides the Brazilian Digital Library Theses and Dissertations (BDBTD). From this survey we selected works that present educational products materialized in blocks of activities based on the use of teaching history of mathematics to the classroom as well as the sequence of activities based on the Teaching of Mathematics. In possession of material, produce a CD-ROM containing the selected activities, in order to help support the work of teachers regarding the use of these activities, as a supplementary material to textbooks in their math classes

Pesquisa em educação ambiental: a atividade de campo em teses e dissertações

Pós-graduação em Educação - IBRC

A dimensão valorativa da educação ambiental na formação continuada de professores apresentada em teses e dissertações brasileiras

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

A ética ambiental na produção teórica (dissertações e teses) em educação ambiental no Brasil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Os projetos de Educação Ambiental desenvolvidos nas escolas brasileiras: análise de diissertações e teses

Pós-graduação em Educação - IBRC

Análise da produção acadêmica constante no banco de teses da Capes segundo o assunto educação de surdos (2005-2009)

Pós-graduação em Educação Escolar - FCLAR

Compreensões elaboradas pelo campo da educação ambiental sobre o tema mudanças climáticas: análise de dissertações e teses brasileiras

Pós-graduação em Educação - IBRC

A representação do ato de ensinar: continuidades e rupturas da concepção de ensino na pedagogia tradicional, na psicologia histórico-cultural e na pedagogia histórico-crítica : uma análise a partir das teses e dissertações no Portal da CAPES

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

As práticas das professoras alfabetizadoras como objeto de investigação: teses e dissertações de programas de pós-graduação em educação do estado de São Paulo (1980 a 2005)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O uso de jogos no ensino e aprendizagem de Matemática nos anos iniciais do Ensino Fundamental: levantamento de teses e dissertações

Pós-graduação em Educação - FCT

Dissertações e teses da pós-graduação em educação na Unesp-Campus de Marília: um estudo das citações e cocitações (2004 a 2009)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Organização do conhecimento em bibliotecas digitais de Teses e Dissertações: análise da aplicabilidade das teorias macroestruturais para categorização de áreas de assunto

Pós-graduação em Ciência da Informação - FFC

Nas malhas do leitor: um estudo de teses e dissertações sobre leitura/recepção de textos (1980-2003)

Pós-graduação em Letras - FCLAS

Estudos da enantio- e estéreo-especificidade da SADH de Thermoanaerobacter ethanolicus 39E: mutagênese, expressão, purificação, cristalização e estudos cristalográficos

Pós-graduação em Biofísica Molecular - IBILCE