Shell midden people in northern Iberia. New data from the mesolithic rock shelter of J3 (Basque Country, Spain)

[EN]In the course of a sondage dug in the rock shelter of J3, in the Jaizkibel mountains (at the north-western tip of Guipúzcoa), the body of a adult man was located buried inside a shell midden. This shell midden had not been disturbed and presented internal stratigraphy features. In any case, the outer edge of the shell midden does show some interesting interdigitation with the adjacent habitational layers, with evidence of different stages of occupation. Within the shell midden itself, under the individual buried there, it was possible to observe layers without any ceramics, whereas the layers covering said individual included ceramic fragments. This individual has been dated to 8,300 BP and therefore corresponds to a Mesolithic context.

Urban rural water exchange : PRW in the Lockyer Valley

A significant amount (ca. 15-25 GL/a) of PRW (Purified Recycled Water) from urban areas is foreseen as augmentation of the depleted groundwater resources of the Lockyer Valley (approx. 80 km west of Brisbane). Theresearch project uses field investigations, lab trials and modelling techniques to address the key challenges: (i) how to determine benefits of individual users from the augmentation of a natural common pool resource; (ii) how to minimise impacts of applying different quality water on the Lockyer soils, to creeks and on aquifier materials; (iii) how to minimuse mobilisation of salts in the unsaturated and saturated zones as a result of increased deep drainage; (iv) determination of potential for direct aquifer recharge using injection wells?

Problems in the assessment of magnesium depletion in the rat by in vivo 31P NMR

Prior in vitro studies, utilizing 31Pn uclear magnetic resonance (31PN MR) to measure the chemical shift (CT) of 0-ATP and lengthening of the phosphocreatine spin-spin (7"') relaxation time, suggested an assessment of their efficacy in measuring magnesium depletion in vivo. Dietary magnesium depletion (Me$) produced markedly lower magnesium in plasma (0.44 vs 1. I3 mmol/liter) and bone (1 30 vs 190 pmol/g) but much smaller changes in muscle (41 vs 45 pmol/g, P < 0.01), heart (42.5 vs 44.6 prnol/g), and brain (30 vs 32 pmollg). NMR experiments in anesthetized rats in a Bruker 7-T vertical bore magnet showed that in M e $ rats there was a significant change in brain j3-ATP shift (16.15 vs 16.03 ppm, P < 0.05). These chemical shifts gave a calculated free [Mg"] of 0.71 mM (control) and 0.48 mM (MgZ+$). In muscle the change in j3-ATP shift was not significant (Me$ 15.99 ppm, controls 15.96 ppm), corresponding to a calculated free M P of 0.83 and 0.95 mM, respectively. Phosphccreatine Tz (Carr-Purcell, spin-echo pulse sequence) was no different with M e $ in muscle in vivo (surface coil) (M$+$ 136, control 142 ms) or in isolated perfused hearts (Helmholtz coil) (control 83, M e $ 92 ms). 3'P NMR is severely limited in its ability to detect dietary magnesium depletion in vivo. Measurement of j3-ATP shift in brain may allow studies of the effects of interaction in group studies but does not allow prediction of an individual magnesium status.

Syntheses of the trans,meso- and racemic C11 acids, degradation products of diterpene acids

Syntheses of the isomers of the C11 acid, 1(a),3(a)- dimethylcyclohexane-1 (e),2(e),3(e)-tricarboxylic acid (A) and 1(a),3(e)-dimethylcyclohexane-1(e),2(e),3(a)-tricarboxylic acid (B), the latter by two different routes, are reported. Two of the four possible isomers of the precursor triester, trimethyl 1-methylcyclohexane-1,2,3-tricarboxylate, on individual methylation followed by hydrolysis, gave the trans,meso-acid (A), identified by comparison with an authentic sample, and the cis,trans-form (B) whose structure and configuration were proved by comparison with a specimen obtained by the unambiguous and highly stereoselective second synthesis. This demonstrated that methylation of the triester isomers occurs stereospecifically and exclusively at C-3. In the second sequence, it has been possible to assign definite conformations to four key intermediates and the final product, directly from n.m.r. spectra, from changes in these spectra accompanying specific steps, and from chemical evidence. Comparison of the n.m.r. spectra of the isomeric triesters (A) and (B) has provided unequivocal proof of the accepted trans,meso configuration for the abietic acid degradation product (A).

Reactions of tetrahalogeno-1,2-benzoquinones. Part III. Reaction of tetrachloro-1,2-benzoquinone with tetralones and naphthols: Pathway to the condensates

With a view to understanding the mechanism of the formation of 6-methoxy-2,2-(tetrachloro--phenylenedioxy)-naphthalen-1 (2H)-one (IIIa) in the reaction of 6-methoxy-1-tetralone (Ia) with tetrachloro-1,2-benzoquinone (II), the reaction of (II) with various tetralones and naphthols has been studied. Reaction with either α-tetralone or α-naphthol gives 2,2-(tetrachloro-o-phenylenedioxy)naphthalen-1 (2H)-one (IIIb), whereas reaction with either β-tetralone or β-naphthol gives a mixture of (IIIb) and ,1-(tetrachloro-o-phenylenedioxy)-naphthalen-2 (1H)-one (IX), with the former predominating. Further, reactions of (II) with 7-methoxy-3,4-dihydrophenanthren- 1 (2H)-one and m-methoxyphenol gave respectively 7-methoxy- ,2-(tetrachloro-o- phenylenedioxy)phenanthren-1 (2H)-one (VII) and 3-methoxy-6,6-(tetrachloro-o- phenylenedioxy)cyclohexa-2,4-dien-1-one (VIII). Structures of all these compounds have been proved on the basis of i.r. and n.m.r. data. The pathway to the formation of the condensates (III) is discussed.

Aspects of tautomerism. Part II. Reactions of the pseudo-acid chloride of o-benzoylbenzoic acid with nucleophiles

Reactions of fourteen nucleophiles with the pseudo-acid chloride of o-benzoylbenzoic acid in two solvents have been studied. The nucleophiles that react primarily at the tetrahedral carbon atom to give pseudo derivatives, are weaker than those that react at the carbonyl carbon atom causing opening of the lactone ring. An explanation for this phenomenon is advanced.

Stereoselectivity in the addition of hydrogen chloride to cyclohex-1-enecarbonitrile: Possible role of chlorine lone pair-nitrile π-orbital pseudoallylic A1, 3 interaction

The (overall trans) addition of hydrogen chloride to cyclohex-1- enecarbonitrile in anhydrous alcoholic media proceeds to give cis-2-chlorocyclohexanecarboxylate (together with some cis-2- chlorocyclohexanecarboxamide): no corresponding products with the trans-configuration are detectable. In anhydrous ether the addition proceeds to give a single isomer, presumably cis-, of 2-chlorocyclohexanecarbonitrile, indicating that the configuration of the products may not be equilibrium-controlled in alcoholic media. An examination of the steric factors indicates that the transition state for protonation of the presumed intermediate, 2-chlorocyclohexylidenemethylideneimine, leading to cis-product is favoured if interaction between the lateral π-orbital of the C-N double bond and the lone-pairs on the chlorine atom at the 2-position is large. Consideration of interactions in the transition states meets Zimmerman's criticism that invoking A1, 3 interaction existing in ground states to explain product configuration takes insufficient account of the Curtin-Hammett principle.

Aspects of tautomerism. Part III. A new dimerisation reaction of pseudo-acid chlorides

Pseudo-acid chlorides of five 4′-substituted o-benzoylbenzoic acids are converted into a mixture of dilactones with sodium iodide in acetone. The meso-isomer is always formed to a larger extent than the (±)-mixture. These results imply that the radicals involved are not planar.

CIMS信息集成环境下轧钢企业财务管理系统的实现

以鞍钢冷轧厂计算机集成制造系统开发为背景 ,介绍了基于Client/Server方式 ,以OR ACLE为后台数据库的财务管理系统的设计与实现。运用软件工程和数据库理论 ,结合ORACLERDBMS的特点 ,并对实现方法做了比较全面地论述。最后 ,探讨了通向财务信息集成的途径

四川盆地构造控藏规律研究

Firstly, established sequence stratigraphy of Sinian System-Middle Triassic Series framework in Sichuan basin,be divided into 21 second-level sequence stratigraphy and 105 third-level sequence stratigraphy.From many aspects,discussed sequence stratigraphy characteristic. On the foundation of structure unconformity and fission track analysis, on the ground of An county-Shuinin county regional seismic section, using the positive evolution equilibrium principle technology, comprehensivly be mapped structure evolution of Sichuan basin. It can be divided into seven stages, that is :Pre-Sinian basement stage, cratonic depression basin(Z1-S)stage, cratonic rifted basin(D-T2)stage, passive continental margin(T3x1-3)stage, foreland basin(T3x4-6)stage, depression basin (Jurassic Period-Miocene Epoch) stage, formed basin (Holocene Epoch) stage. Analysis on structure evolution history,burial history,source rocks thermal evolution history, Maoba changxing formation gas pool forming process can be classified into four stages: ancient lithological oil pool stages in Indosinian-early Yanshanian period（T-J1-2）, ancient structure- lithological gas pool stages in middle Yanshanian period（J3-K1）, structure- lithological gas pool setting stages in last Yanshanian period（K2）, structure- lithological gas pool adjusting and transformation stages in Himalayan period（R-Q）. Maoba feixianguan formation gas pool forming process can be classified into two stages: second structure gas pool stages in last Yanshanian period（K2）,second structure gas pool physical adjusting and transformation stages in Himalayan period（R-Q）,and summarize reservoir formation model. On the base of newest exploration achievement and petroleum geologic comprehensive research , demonstrate how structure controls hydrocarbon accumulation. Structure controlling source rocks behaves structure controlling main source rocks’sedimentary facies, medium-large pools mainly located at center or margin of hydrocarbon generation. Structure controlling palaeo-karst reservoirs ,reef and beach facies reservoirs, fault and fracture reservoirs. Structure controlling palaeo-uplift, and palaeo-uplift controlling hydrocarbon migration, active reservoirs’forming, palaeo-structure traps forming. Structure controls distribution of mudstone and gypsolith, controls preservation. Structure controls hydrocarbon conducting, structure traps forming and hydrocarbon accumulation. Whether or no, Structure controls total process of basin forming-source rocks’generation- hydrocarbon accumulation. It is direct effect results of structure movements that large traps’ conditions, conducting migration conditions, high quality preservation. source rocks’condition and reservoirs’ condition are the indirect effect results. In the last analysis, “source rock controlling theory”, “high quality reservoir mainly controlling theory”, “palaeo-uplift controlling theory” and “current structure deciding theory” are structure controlling hydrocarbon accumulation. There are high variability and complex mechanisms in Sichuan basin , but the regional hydrocarbon accumulation conditions are very well, such as abundant source rocks, matching process of hydrocarbon accumulation and many exploration areas. By means of integrated analysis, put forward hydrocarbon exploration direction and large-middle targets of China Petroleum and Chemical Corporation .Thus, more and more hydrocarbon proved reserve and output in Sichuan basin will be contributed to China energy industry in a long future time.

下扬子北缘前陆盆地形成演化与油气赋存条件

The foreland basin on the northern margin of the lower reach of the Yangtze river (the lower Yangtze foreland basin) is tectonically situated in the basin-mountain transitional area along the southeastern flank of the Dabie mountains. The early formation and development of the basin is closely related to the open-up of the Mian-Lue paleo-oceanic basin on the southern margin of the Central Orogenic System represented by Qinling-Dabei orogenic belt, while the tectonic evolution of the middle-late stage of the basin is mainly related to development of the Mian-Lue tectonic zone that occurred on the basis of the previous Mian-Lue paleo-suture. The foreland basin of the northern rim of the lower reach of the Yangtze river was formed during the middle-Triassic collision between the Yangtze and North China plates and experienced an evolution of occuirence-development-extinction characterized by marine facies to continental facies and continental margin to intracontinent in terms of tectonic setting.The foreland basin (T2-J2) was developed on the basis of the passive continental marginal basin on the south side of the Mian-Lue paleo-ocean and superimposed by late Jurassic-Tertiary fault basin. The tectonic setting underwent a multiple transformation of rifting-collisional clososing-tensional faulting and depression, which resulted in changes of the property for the basin and the final formation of the superposed compose basin in a fashion of 3-story-building. According to the tectonic position and evolution stages of plate collision happening on the southeastern margin of the Dabie mountains, and tectono-tratigraphic features shown by the foreland basin in its main formational period, the evolution of the foreland basin can be divided into four stages: 1) pre-orogenic passive margin (P2-Ti). As the Mian-Lue ocean commenced subduction in the late-Permian, the approaching of the Yangtze and North China plates to each other led to long-periodical and large-scale marine regression in early Triassic which was 22 Ma earlier than the global one and generated I-type mixed strata of the clastic rocks and carbonate, and I-type carbonate platform. These represent the passive stratigraphy formed before formation of the foreland basin. 2) Foreland basin on continental margin during main orogenic episode (T2.3). The stage includes the sub-stage of marine foreland basin (T2X remain basin), which formed I-type stratigrphy of carbonate tidal flat-lagoon, the sub-stage of marine-continental transition-molasse showing II-type stratigraphy of marine-continental facies lake - continental facies lake. 3) Intracontinental foreland basin during intracontinental orogeny (Ji-2)- It is characterized by continental facies coal-bearing molasses. 4) Tensional fault and depression during post-orogeny (J3-E). It formed tectono-stratigraphy post formation of the foreland basin, marking the end of the foreland evolution. Fold-thrust deformation of the lower Yangtze foreland basin mainly happened in late middle-Jurassic, forming ramp structures along the Yangtze river that display thrusting, with deformation strength weakening toward the river from both the Dabie mountains and the Jiangnan rise. This exhibits as three zones in a pattern of thick-skinned structure involved the basement of the orogenic belt to decollement thin-skinned structure of fold-thrust from north to south: thrust zone of foreland basin on northern rim of the lower reach of the Yangtze river, foreland basin zone and Jiannan compose uplift zone. Due to the superposed tensional deformation on the earlier compressional deformation, the structural geometric stratification has occurred vertically: the upper part exhibits late tensional deformation, the middle portion is characterized by ramp fault -fold deformation on the base of the Silurian decollement and weak deformation in the lower portion consisting of Silurian and Neo-Proterozoic separated by the two decollements. These portions constitutes a three-layered structural assemblage in a 3-D geometric model.From the succession of the lower reach of the Yangtze river and combined with characteristics of hydrocarbon-bearing rocks and oil-gas system, it can be seen that the succession of the continental facies foreland basin overlies the marine facies stratigraphy on the passive continental margin, which formed upper continental facies and lower marine facies hydrocarbon-bearing rock system and oil-gas forming system possessing the basic conditions for oil-gas occurrence. Among the conditions, the key for oil-gas accumulation is development and preservation of the marine hydrocarbon-bearing rocks underlying the foreland basin. The synthetic study that in the lower Yangtze foreland basin (including the Wangjiang-Qianshan basin), the generation-reservoir-cover association with the Permian marine facies hydrocarbon-bearing rocks as the critical portion can be a prospective oil-gas accumulation.Therefore, it should aim at the upper Paleozoic marine hydrocarbon-bearing rock system and oil-gas forming system in oil-gas evaluation and exploration. Also, fining excellent reservoir phase and well-preserved oil-gas accumulation units is extremely important for a breakthrough in oil-gas exploration.

湘东北中生代望湘花岗岩体岩石地球化学特征及其构造环境

华南中生代构造由早期的挤压转为晚期的拉张，确定这种构造转折时期对认识华南大地构造演化具有重要意义。花岗岩的多期次侵入往往是不同构造环境的产物，不同构造环境花岗岩表现出岩石地球化学组成上的差别，本文对湘东北望湘花岗岩体不同期次岩石地球化学研究后，认为257－165Ma,151-144Ma,135-128Ma3个侵入期的花岗岩岩石分别在TiO2,T/Rb,Rb/Sr,∑REE,δEu及微量元素组成上存在较大的差异，反映为不同的产出构造环境，分别代表印支期的挤构造环境，燕山早期的剪切一走滑环境和燕山晚期的伸展－滑脱环境，J3－K1（140Ma±）是挤压－剪切与伸展－拉张构造环境的转折期，前者表现为岩石圈增厚，后者表现为岩石圈减薄，湘东南大规模金属及铀矿的形成多与岩石圈减薄期花岗质小岩有成因联系。

准噶尔盆地构造演化与油气成藏特征

对准噶尔盆地构造特征、地层分布和地层不整合等特征进行的研究结果表明，该盆地内经历了裂陷盆地、碰撞前陆盆地、陆内坳陷盆地和陆内俯冲前陆盆地等4个演化阶段。裂陷盆地控制了石炭系烃源岩，使其沿裂陷槽分布；碰撞前陆盆地将二叠系烃源岩局限于玛湖、昌吉、克拉美丽山等山前坳陷内；陆内坳陷期从三叠纪一直持续到古近纪末，盆地内泥岩和煤岩广泛分布；陆内俯冲前陆盆地发育在南缘西部。因而，古、新近系烃源岩主要分布在安集海—呼图壁一带。烃源岩演化和油源对比表明，盆地内有3次成藏期，分别为印支期(T3)、燕山期(J3)和喜山期(E2)，形成了石炭系、二叠系、侏罗系和古近系4个复合含油气系统。根据构造特征和成藏条件的差异性，在盆地内可划分出西北部、西部、南部、东部和腹部5个油气聚集区带。准噶尔盆地的成藏模式主要有逆冲断阶-不整合单向充注、压扭背斜双向充注、断隆带双向充注、披覆背斜单向充注、逆冲背斜带单向充注和斜坡带单向充注6种类型。

Morphobiometric and molecular characterization of Bursaphelenchus Fuchs, 1937 (Nematoda: Aphelenchoididae) species associated with Pinus pinaster Ainton in Portugal

Abstract - The genus Bursaphelenchus comprises almost 100 species mainly from the northern hemisphere, with conifers as the most important hosts. Among the various nematode species, the pine wood nematode (PWN), Bursaphelenchus xylophilus, is the casual agent of pine wilt disease (PWD), and the most important forest pest for pines worldwide, classified as an A1 quarantine organism within the European Union. In 1999 this nematode was detected for the first time in Portugal and Europa associated with maritime pine, Pinus pinaster. Following detection, a national program denominated "Programa Nacional de Luta contra o Nemátodo da Madeira do Pinheiro" (PROLUNP) was created to, among other objectives, determine the distribution of the PWN and its associated vector(s) and host(s), and therefore intensive surveys covering the entire country were conducted with thousands of wood samples and suspected insects being analyzed. This thesis presents the listing, distribution, frequency and the insects associated with Bursaphelenchus species found associated with maritime pine in Portugal, identifying and characterizing the various species by morphological, biometrical and molecular biology (ITS-RFLP and rDNA sequencing analysis) techniques. To achieve the objectives, a total of 4813 maritime pine wood samples and 3294 insects from 22 species and six families were individually analyzed. A total of nine Bursaphelenchus species were found, namely: B. antoniae, B. hellenicus, B. leoni, B. mucronatus, B. pinasteri, B. sexdentati, B. teratospicularis, B. tusciae and B. xylophilus, all of them (with the exception of B. xylophilus) being new records for Portugal. Some of the species appear to have a widespread distribution, such as B. leoni, B. teratospicularis and B. tusciae while others were very rarely found and apparently have a localized distribution range within the country, namely B. antoniae and B. mucronatus. The majority of the species is characteristic of the Mediterranean region and can also be found in countries such as Spain, Italy and Greece, reflecting the affinity of our fauna with those locations. The association of B. hellenicus and B. tusciae with maritime pine is here reported for the first time. Six of the Bursaphelenchus species were also found associated with insects, mainly from the family Scolytidae (Coleoptera). Some of these interactions were described for the first time, namely: B. hellenicus with both Ips sexdentatus and Hylurgus ligniperda, B. sexdentati with both H. ligniperda and Orthotomicus erosus and B. tusciae with H. ligniperda. The exclusive association of B. xylophilus with the cerambycid Monochamus galloprovincialis was also confirmed. The nematode's dauer juveniles were usually found in low numbers in the insect vectors (ca 10-100 per insect), although for B. xylophilus a few thousand specimens per insect were sometimes found. The location of the dauer juveniles differed according to the species, although they were more common under the elytra and wings of the adult insects. A species new to science was detected and formally described as B. antoniae, associated with Hylobius sp. (Coleoptera; Curculionidae) beetles. Morphologically, this new species is very similar to B. hylobianum, although it's distinct ITS-RFLP molecular pattern (with only the enzyme Haelll producing comparable restriction bands) and the failure of hybridization supported the two species as distinct entities. Additional phylogenetic analysis of the 18S rDNA sequence further supported the taxonomical proximity of B. antoniae with B. hylobianum. Concerning the PWN, detailed studies on the development and morphology of B. xylophilus were conducted, and comparative measurements of field-collected and laboratory-maintained populations demonstrated that nematodes from the second group displayed larger size in all morphometric parameters, which could derive from more adequate conditions of nourishment and/or temperature. Taxonomical studies on the development stages of B. xylophilus confirmed the existence of four propagative juvenile stages (J1,J2,J3 and J4), an adult stage with both sexes and two dispersal stages (jIII e jIV), with the measurements of the gonad length allowing the separation of the propagative stages. It is hoped that the acquired knowledge will be useful on future surveys of nematodes of the Bursaphelenchus genus collected from either wood material or insect vectors, and facilitate the correct distinction and identification of the various species which are now known to occur. ### Resumo - 0 género Bursaphelenchus compreende quase 100 espécies, distribuídas sobretudo nos países do hemisfério norte do globo terrestre. Embora algumas espécies já tenham sido detectadas em plantas herbáceas, os hospedeiros vegetais mais comuns deste género são as coníferas, particularmente pinheiros. 0 nemátode da madeira do pinheiro (NMP), Bursaphelenchus xylophilus, é considerado a espécie mais importante deste género uma vez que é o agente causal da doença da murchidão dos pinheiros ("pine wilt disease"). Originário dos Estados Unidos, onde não causa grande impacte, o NMP foi introduzido em alguns países da Ásia (China, Japão, Coreia e Taiwan) e mais recentemente na Europa (Portugal). Nestas regiões é responsável pela destruição de milhares de hectares de coníferas, assumindo uma elevada importância económica. Em Portugal, depois da sua detecção em 1999, associado a Pinus pinaster, foi implementado um programa nacional "Programa Nacional de Luta contra o Nemátodo da Madeira do Pinheiro" (PROLUNP) que permitiu determinar a área afectada pela praga (a sul do rio Tejo, península de Setúbal) bem como definir e implementar estratégias de controlo e prevenção da disseminação do NMP a outras zonas de Portugal. Recentemente, em Junho de 2008, foi confirmada a presença de B. xylophilus em outras regiões de Portugal levando as autoridades oficiais a definir todo o território continental como zona afectada e de restrição. As prospecções intensivas realizadas nos últimos anos incluíram a recolha e análise de milhares de amostras de madeira de pinheiro bem como de insectos associados ao pinheiro bravo conduzindo à identificação de várias espécies de Bursaphelenchus. Assim, os estudos conduzidos neste trabalho tiveram como objectivos efectuar uma caracterização morfológica, biométrica e molecular das espécies associadas a P. pinaster em Portugal bem como a sua distribuição geográfica e abundância. Os estudos biométricos foram realizados com populações extraídas directamente do meio natural. Foi ainda realizada uma pesquisa que permitiu identificar os insectos a que estão associadas essas espécies, os seus possíveis vectores. Foram analisadas no total 4813 amostras de P. pinaster e 3294 insectos (22 espécies pertencentes a seis famílias diferentes). Foram identificadas um total de nove espécies: B. antoniae n. sp., B. hellenicus, B. leoni, B. mucronatus, B. pinasteri, B. sexdentati, B. teratospicularis, B. tusciae e B. xylophilus. Foram realizados estudos morfológicos e biométricos de todas as espécies com excepção de B. mucronatus; o reduzido número de exemplares encontrados em apenas uma amostra foram utilizados para efectuar o diagnóstico molecular desta espécie (ITS-RFLP). Apesar de ter havido, sempre que possível, a confirmação molecular, na maioria dos casos a caracterização morfológica e biométrica permitiu a correcta identificação das espécies. Contudo, foi imprescindível a análise molecular em algumas amostras, nomeadamente para a identificação de B. xylophilus e B. sexdentati; dada a grande semelhança entre B. xylophilus e B. mucronatus e tendo sido encontradas algumas populações de B. xylophilus que possuíam fêmeas com cauda mucronada, foi necessária a realização da confirmação molecular. Com excepção de B. xylophilus, todas as outras espécies foram reportadas pela primeira vez em Portugal. Juntamente com B. xylophilus, B. pinasteri foi a espécie encontrada nas amostras de madeira de pinheiro com maior frequência. Algumas destas espécies como B. leoni, B. teratospicularis e B. tusciae foram reportadas em diferentes localidades do norte, centro e sul de Portugal, apresentando uma vasta distribuição geográfica; este resultado está em consonância com a forte associação destas espécies a climas mediterrânicos tal como acontece em Espanha, França, Itália e Grécia. Em oposição, espécies como B. antoniae e B. mucronatus foram encontradas apenas numa ocasião na região centro (Leiria) e norte (Figueira da Foz) do país, respectivamente. Bursaphelenchus mucronatus é igualmente pouco frequente em Espanha onde ocorre sobretudo na região norte, na Galiza. Esta espécie preferirá climas mais frios, ocorrendo com uma maior frequência nas regiões de latitude norte; esta análise é corroborada pela presença constante em países como Alemanha, Finlândia, França, Noruega, Rússia e Suécia. A nível mundial são descritas neste trabalho pela primeira vez as associações das espécies B. hellenicus e B. tusciae ao hospedeiro vegetal P. pinaster.