Results of hydrologic tests and water-chemistry analyses, wells H-5A, H-5B, and H-5C, at the proposed waste isolation pilot plant site, southeastern New Mexico /

"February 1982."

Geologic and well-construction data for the H-9 borehole complex near the proposed Waste Isolation Pilot Plant site, southeastern New Mexico /

GPO copy lacks plate.

Report of workshop on lung cell identification, isolation and culture, March 2-3, 1973, Seattle, Washington.

Mode of access: Internet.

Isolation and identification of Salmonella [and] Shigella /

Tables.

The direct isolation and cultivation of human bovine and avian tubercle bacilli /

Thesis (M. S.) - Cornell University, June 1920.

A 25-kV bias-isolation unit for 1-MHz capacitance and conductance measurements /

Includes bibliographical references.

Operator and organizational maintenance fault isolation manual : improved CW acquisition radar AN/MPQ-55, (Improved HAWK Air Defense Guided Missile System).

"September 1980."

Operator and organizational maintenance fault isolation manual : improved high-powered illuminator radar AN/MPQ-46 (XO-1), (improved Hawk air defense guided missile system).

"January 1979."

Nuclear waste : storage issues at DOE's waste isolation pilot plant in New Mexico : report to the Chairman, Environment, Energy, and Natural Resources Subcommittee, Committee on Government Operations, House of Representatives /

"GAO/RCED-90-1."

From isolation to participation /

Cover title.

The electron, its isolation and measurement and the determination of some of its properties.

A reprint of the original 1917 edition.

Diagnostic microbiology : a textbook for the isolation and identification of pathogenic microorganisms /

Mode of access: Internet.

Isolation and characterization of a Cotesia rubecula bracovirus gene expressed in the lepidopteran Pieris rapae

Polydnaviruses are endogenous particles that are crucial for the survival of endoparasitoid wasps, providing active suppression of the immune function of the lepidopteran host in which wasp larvae develop. The Cotesia rubecula bracovirus (CrBV) is unique in that only four gene products are detected in larval host (Pieris rapae) tissues and expression of CrBV genes is transient, occurring between 4 and 12 h post-parasitization. Two of the four genes, CrV1 and CrV3, have been characterized. CrV1 is a secreted glycoprotein that has been implicated in depolymerization of the actin cytoskeleton of host haemocytes, leading to haemocyte inactivation; CrV3 is a multimeric C-type lectin that shares homology with insect immune lectins. Here, a third CrBV-specific gene is described, CrV2, which is expressed in larval P. rapae tissues. CrV2, which is transcribed in haemocytes and fat body cells, has an ORF of 963 bp that produces a glycoprotein of approximately 40 kDa. CrV2 is secreted into haemolymph and appears to be internalized by host haemocytes. CrV2 has a coiled-coil region predicted at its C-terminus, which may be involved in the formation of putative CrV2 trimers that are detected in haemolymph of parasitized host larvae.

Isolation of an imaginal disc growth factor homologue from Pieris rapae and its expression following parasitization by Cotesia rubecula

Endoparasitoid insects introduce maternal factors into the body of their host at oviposition to suppress cellular defences for the protection of the developing parasitoid. We have shown that transient expression of polydnavirus genes from a hymenopteran parasitoid Cotesia rubecula (CrPDV) is responsible for the inactivation of hemocytes from the lepidopteran host Pieris rapae. Since the observed downregulation of CrPDV genes in infected host tissues is not due to cis-regulatory elements at the CrV1 gene locus, we speculated that the termination of CrPDV gene expression may be due to cellular inactivation caused by the CrV1-mediated immune suppression of infected tissues. To test this assumption, we isolated an imaginal disc growth factor (IDGF) that is expressed in fat body and hemocytes, the target of viral infection and expression of CrPDV genes. Time-course experiments showed that the level of P. rapae IDGF is not affected by parasitization and polydnavirus infection. However, the amount of highly expressed genes, such as storage proteins, arylphorin and lipophorin, are significantly reduced following parasitization. (C) 2004 Elsevier Ltd. All rights reserved.