965 resultados para Inspeção sanitária


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Pós-graduação em Medicina Veterinária - FCAV

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Pós-graduação em Medicina Veterinária - FMVZ

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A inspeção sanitária da carne bovina e suína tem sido a principal forma diagnóstica da cisticercose animal e da prevenção da teníase no Brasil. As aldeias indígenas Jaguapirú e Bororó estão localizadas próximo à área urbana do município de Dourados-MS, com condições precárias de saneamento básico, onde bovinos e suínos são criados como fonte de alimento para consumo próprio, bem como para comercialização externa, geralmente sem inspeção sanitária oficial. Neste estudo, 96 carcaças bovinas e 117 amostras de soro de suínos, criados nas aldeias indígenas, foram avaliadas para a presença de formas metacestóides à inspeção sanitária e de anticorpos anti-Taenia sp. ao teste ELISA, respectivamente. Observaram-se 18,75% de positividade para cisticercose bovina e 9,4% para cisticercose suína. A ocorrência do complexo teníase-cisticercose nas aldeias pode favorecer a ocorrência desta zoonose na população indígena. Condições adequadas de abate e inspeção sanitária dos animais destas aldeias se fazem urgente para o controle do complexo teníase-cisticercose na população indígena.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Pós-graduação em Medicina Veterinária - FMVZ

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Post-mortem bacterial culture and specific biochemical tests are currently performed to characterize the etiologic agent of bovine tuberculosis. Cultures take up to 90 days to develop. A diagnosis by molecular tests such as PCR can provide fast and reliable results while significantly decreasing the time of confirmation. In the present study, a nested-PCR system, targeting rv2807, with conventional PCR followed by real-time PCR, was developed to detect Mycobacterium tuberculosis complex (MTC) organisms directly from bovine and bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other Actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. Regarding the analytical sensitivity, DNA of the M. bovis AN5 strain was detected up to 1.5 pg by nested-PCR, whereas DNA of M. tuberculosis H37Rv strain was detected up to 6.1 pg. The nested-PCR system showed 100% analytical specificity for MTC when tested with DNA of reference strains of non-tuberculous mycobacteria and closely-related Actinomycetales. A clinical sensitivity level of 76.7% was detected with tissues samples positive for MTC by means of the culture and conventional PCR. A clinical specificity of 100% was detected with DNA from tissue samples of cattle with negative results in the comparative intradermal tuberculin test. These cattle exhibited no visible lesions and were negative in the culture for MTC. The use of the nested-PCR assay to detect M. tuberculosis complex in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Tuberculosis infection among humans transmitted by products of animal origin can be caused by Mycobacterium bovis, a concern in developing countries, because the number of human cases is relatively high compared with countries that have implemented programs to eradicate bovine tuberculosis for many years. Because it is a chronic disease with subclinical evolution, it is transmitted to other animals in the herd livestock resulting in high loss losses resulting high (10 to 25%), as well as the zoonotic nature of the disease on public health. This work aimed to study the histomorphology of granulomas in samples of lymph nodes and lungs of animals with suggestive lesions of tuberculosis, as well as those similar to the sanitary inspection. The animals clinically healthy ante-mortem, were slaughtered in a slaughterhouse in the northwestern state of Sao Paulo between April 2008 and April 2009, considering that carcasses had lymph nodes with lesions typical of tuberculosis, exhibiting nodular and hemorrhagic appearance. The caseous or calcified lesions, purulent or not, of various sizes and shapes, were also evaluated. Of the 307,661 animals slaughtered in the mentioned period, 494 gross suggestive lesions of tuberculosis and from 29 cities in the state of Sao Paulo, 16 from Mato Grosso do Sul, Goias 15, one from Mato Grosso and Paraná. Among these, 200 samples (40,5%) were processed for histopathological evaluation by hematoxylin-eosin (HE) and 90 (45%) were subjected to Ziehl-Neelsen (ZN) and examined by light microscopy. In HE staining, histopathological changes of tuberculosis were found in 197 (98,5%), classified according to the stage of development adopted by Wango et al.(31), where 29 samples were included in stage I (14,5%), 53 in stage II (26,5%), 87 in stage III (43,5%), 28 in stage IV (14%). In the ZN staining Mycobacterium sp was found in 89 (99%) of the specimens. The rate of tuberculosis in the herd studied was 0,16%. We conclude that the identification of the disease in slaughterhouses and meat companies from significant gross lesions, enables the implementation of effective measures, specifically the origin of the disease in cattle herds, providing control measures to prevent its spread.

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Este relatório descreve as atividades desenvolvidas durante o estágio curricular do curso de Mestrado Integrado em Medicina Veterinária no âmbito da inspeção sanitária e saúde pública, realizado tanto nos PIF de Lisboa, como na Divisão de Riscos Alimentares (DRA) da Autoridade de Segurança Alimentar e Económica (ASAE). Existe uma grande variedade de produtos e animais que chegam diariamente à União Europeia provenientes de diferentes países terceiros, como por exemplo a China, África do Sul, Brasil, Índia, Canadá e Senegal. Durante o período de estágio a categoria dos produtos mais inspecionada nos PIF foi o pescado representando 57,5% da totalidade das remessas inspecionadas nos portos de Lisboa e 98% no aeroporto. No âmbito do plano de fiscalização da ASAE em 2015, a aluna analisou 365 amostras de géneros alimentícios e constatou-se que o número de amostras não conformes foi 254. O grupo das bebidas alcoólicas destacou-se com 159 amostras não conformes; Abstract: National Control Plans of Food This essay describes the activities developed throughout the curricular internship of the Integrated Master of Veterinary Medicine within sanitary inspection and public health, both at border control of Lisbon as well as in the Divisão de Riscos Alimentares (DRA) of the Autoridade de Segurança Alimentar e Económica (ASAE). There is a wide variety of products and animals that arrive daily to the European Union from third countries, such as China, South Africa, Brazil, India, Canada and Senegal. During the internship the largest category of inspected food was fish representing 57.5% of the inspected remittances in the port of Lisbon and 98% in the airport. As part of the fiscalization plan of ASAE in 2015 the student analysed 365 food samples and it was found that the number of samples non-conforming was 254. The ardent spirits was the category that stood out with 159 non-conforming samples (63%).

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Consultoria Legislativa - Área X - Agricultura, Pecuária, Abastecimento e Desenvolvimento Rural. Nome do autor na capa: Rodrigo H. C. Dolabella.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)