Comparação da infiltração marginal de restaurações adesivas empregando instrumento rotativo ou um sistema químico-mecânico na remoção do tecido cariado

Pós-graduação em Odontologia Restauradora - ICT

Métodos geoelétricos empregados na caracterização e monitoramento de anomalias inerentes à infiltração controlada de vinhaça

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Infiltração de água em um podzólico vermelho-escuro sob dois métodos de preparo, usando chuva simulada com duas intensidades

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Participação das NETs (neutrophil extracellular traps) na atividade fungicida de neutrófilos humanos contra o Paracoccidioides brasiliensis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise dos processos de infiltração, percolação, retenção e evaporação em solos arenoso e residuais utilizando lisímetro

The mobility of water in the soil is a difficult process to be quantified. During precipitation, there is a portion of the water that infiltrates, which is very important for recovering the groundwater balance. This work studies the mobility of water in sandy soils with different textures and residual soils through experiments mounted in lysimeters. The lysimeters were mounted on acrylic cylinder with 11.3 cm diameter and 25.0 cm in length. The sand was deposited using the process sand rain. In the tests it was maintained a hydraulic head of two centimeters above the surface with the aid of a Mariotte flask. The portions of water were controlled in the experiments using relations between weight and volume measurements over time. The data were obtained by monitoring the input and output volumes of water through calibrated reservoir and system weight by an electronic balance. The infiltration front was monitored with the aid of a digital camera. The results indicate that the smaller the particle size of the sand, the lower the speed of the saturation front, the higher the retained volume, the smaller the basic rate of infiltration and the lowest speed of percolation. Moreover, the rate of water loss in the evaporation process indicated no significant difference with variation of the particle size of the samples

Infiltração coronária microbiana em canais radiculares obturados pela técnica do cone único com diferentes cimentos endodônticos

With the emergence of new filling materials with different properties and behaviors, the approach of endodontic treatment must be readjusted so that the appropriate result can be achieved. New endodontic sealers include methacrylate resin-based, plant resin-based and the evolution of epoxy-based sealers. This study verified the behavior of new materials that presents controversial results in the literature, about coronal bacterial leakage. That for, 56 single-rooted human teeth were prepared in the direction crown-apex and filled with gutta-percha points with taper of 4% using the single cone technique. Roots were divided randomly into 4 groups according to the sealer (Apexit Plus, AH Plus, EndoREZ and Polifil). After filling, the roots were incorporated in a leakage model, which upper chamber contained a suspension of Streptococcus mutans, and lower chamber a broth, leaving 3 mm of root apical portion immersed. Leakage was assessed for turbidity in lower chamber every day for 60 days. Survival analysis was performed using the nonparametric Kaplan- Meier method (p<0,05). All experimental groups presented leakage during the study’s period, however the maximum time achieve was 22 days. The medium time of leakage was: Apexit Plus 6,3 days, AH Plus 6,3 days and Polifil 5,1 days, but in EndoREZ all specimens infiltrated in the first day, presenting shorter capacity of impermeabilization compared to the other groups. Concluding that none of the sealers tested was able to prevent coronal bacterial leakage

Síntese e estudo da diapocinina como um inibidor da NADPH Oxidase em neutrófilos e células mononucleares de sangue periférico

Apocynin, a methoxy-catechol originally extracted from the root of Picrorhiza kurroa, has been used as an inhibitor of the NADPH oxidase complex in phagocytic and nonphagocytic cells. Its mechanism of inhibition is linked to their prior activation through the action of peroxidases leading to oxidation of the dimeric product, diapocynin. In this study, dipocinina was synthesized and investigated its effect as an inhibitor of activation NADPH oxidase in neutrophils (PMN) and peripheral blood mononuclear cells (PBMC). The synthesis of diapocinina was performed by oxidation of apocinina by potassium persulphate in the midst of water for 5 minutes at room temperature. The precipitate was filtered and washed with water and methanol. Diapocinina was characterized by mass spectrometry. PMN and PBMC were obtained from peripheral blood of healthy donors and purified for gelatin sedimentation, or centrifugation with Histopaque ®, the red cells were lysed with ice water or ammonium chloride. Diapocinina or apocinina were incubated with opsonized zymosan, activation of PMNs and release of superoxide anion, these monitored by chemiluminescent assay dependent lucigenina. We found that diapocinina inhibitor was no better than the apocinina in PMN. However, diapocinina was more efficient than apocinina as an inhibitor of NADPH oxidase in PBMC. In conclusion, whereas PBMC are relatively poor compared with peroxidases PMN, our results are consistent with the need for oxidation apocinina for its effect as an inhibitor of NADPH oxidase

Apocinina versus diapocinina como inibidor da produção de peróxido de hidrogênio e ácido hipocloroso por neutrófilos ativados

Apocynin has been used as an efficient inhibitor of the multi-enzymatic complex NADPH oxidase in many experimental models involving phagocytic and nonphagocytic cells. The mechanism of inhibition has been linked with the previous activation of apocynin through the action of cellular peroxidases leading to the formation of a dimeric oxidation product, diapocynin. In this study we compared apocynin with pure diapocynin regarding their effects as scavenger of hydrogen peroxide and hypochlorous generated by glucose/glucose oxidase and myeloperoxidase respectively, and as inhibitors of the production of hydrogen peroxide and hypochlorous acid by activated neutrophils. The production of hydrogen peroxide was measured by the oxidation of the fluorescent substance Amplex Red and the production of hypochlorous acid by was measured as taurine-chloramine derivative using the chromogenic substrate 3,3’,5,5’- tetramethylbenzidine (TMB). Neutrophils (1 x106 cells/mL) were pre-incubated in PBS buffer supplemented with 1 mM calcium chloride, 0.5 mM magnesium chloride, 1 mg/mL glucose and 5 mM taurine in the presence or absence of inhibitors. The reactions were triggered by adding the soluble stimulus Forbol Miristate Acetate PMA or zymosan and incubated by additional 30 minutes. We found that pure diapocynin was not better than apocynin regarding its scavenger and inhibitory properties. These results suggest that the formation of diapocynin is not essential for the action of apocynin as inhibitor of NADPH oxidase activation

Subsídios para implantação de trincheiras de infiltração na área urbana de Rio Claro/SP

The purpose of this work is to study the theme “infiltration trenches” in some of its main aspects, such as sizing methods and parameters related to this, in order to subsidize the installation of these structures in the urban area of Rio Claro/SP. For purposes of sizing, the “rain-envelope method” was used for its simplicity and direct application from the local characterization data and the IDF (intensity-duration-frequency) curve data. The method bases on the determination of the tributary volume of input and output device. The curve of values accumulated over time of the volumes tributaries to the device, which is built on the flow rates obtained from the local IDF curve, is compared with the value curve of his effluent volumes determined from the flow characteristics obtained from the infiltration soil. The maximum difference between the curves is the volume sizing. Five locations were chosen in the urban area of Rio Claro for implementation of these devices, considering the soil type, hydraulic conductivity of each area and lot size according to the Master Plan of Rio Claro. This work also presented an estimated reduction of the runoff in urban lots by using infiltration trenches.

Avaliação do envolvimento do receptor dectina-1 na atividade fungicida e na produção de metabólitos do oxigênio por neutrófilos e monócitos humanos desafiados com Paracoccidioides brasiliensis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Infiltração bacteriana nas interfaces entre implantes e pilares: efeito do desenho das conexões protéticas

Pós-graduação em Odontologia Restauradora - ICT

Identificação de Neutrophil Extracellular Traps em lesões tegumentares de pacientes com Paracoccidiodomicose e análise da formação de NETs in vitro por neutrófilos humanos desafiados com Paracoccidioides brasiliensis

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Influência do cimento obturador e agente de irrigação final na infiltração marginal em situações de sobreobturações endodônticas

Objective: The objective of this paper was to verify if the final irrigation at 17% EDTA, separately or followed by chlorhexidine digluconate at 2% interferes on the apical marginal leakage in root canal overfilling, due to the use of two root canal filling materials (Sealer 26TM or SealapexTM). Methods: Forty lower incisors extracted, with a single root canal, were biomechanically prepared, at 2.0mm beyond the radicular apex, with ProTaper SystemTM, finishing it with the F3 instrument. Irrigation was accomplished with 1.0 mL NaOCl at 2.5% at each change of equipment caliper and, in the end, with 5.0 mL normal saline solution. After achieving this procedure, the foraminal channels was standardized with a file K 25 until its DO appears in the foraminal opening. From this moment, the teeth were subdivided into two groups of 20 specimens each, because of the final irrigation method used: I – irrigation with EDTA at 17% for 3min and II – identical protocol, thus, after EDTA aspiration at17%, it was again irrigated with chlorhexidine digluconate at 2%, also kept intra-canal for 3min. Each group was again sub-divided into two sub-groups (ten teeth each), according to the cement used (Sealer 26TM or SealapexTM), and filled by using a single gutta-percha F1 (ProTaperTM, Universal Filling), in such a manner that it goes 2.0 mm beyond the radicular apex. Soon after that, the teeth were immersed in Rhodamine BTM for 72h, vacuum and after the roots have been bucolingually grooved, the leakages marked were measured with program Image ToolTM. Results and Conclusions: The leakage magnitude was similar among themselves (p > 0.05), except for group Sealapex®, thus the final use with chlorhexidine reduced apical leakage (p < 0.05).

Avaliação do pH, da solubilidade e da infiltração marginal em retrobturações com o Sealer 26 puro ou acrescido de iodofórmio

Sealer 26® cement contains bisphenol epoxy resin associated with calcium hydroxide, presenting smaller radiopacity than other endodontic cements. Aiming to improve this property, iodoform has been added in its composition. However, this addition's possible changes in physical and chemical properties still need to be studied. OBJECTIVE: To evaluate the apical sealing ability, solubility, and pH of Sealer 26® alone or with iodoform, at several proportions. MATERIAL AND METHODS: Three experimental mixtures of Sealer 26®, alone or with iodoform, were prepared and subjected to solubility test. Additionally, these combinations were inserted into polyethylene tubes and immersed in distilled water, and, their pH was evaluated after 24-h and 7-day periods. Subsequently, forty roots of extracted lower incisors subdivided into four groups of 10 specimens each, were retrograde filled with one of the previously described mixtures and gutta-percha points. The roots were immersed in Rhodamine B, under vacuum, for 72 hours. After this period, the specimens were longitudinally sectioned, root fragments photographed, these images scanned, and apical infiltration measured by Image tool software. The obtained data were subjected to statistical analysis, at a significance level of 5%. RESULTS: Marginal leakage and solubility tests did not show any difference among the experimental groups (p > 0.05). pH analysis was only statistically different at 24-h period and between Sealer 26® alone and 1.1g iodoform group (p < 0.05). CONCLUSION: The presence of iodoform in Sealer 26®, at the used proportions, did not alter the solubility, apical marginal leakage and pH properties of the original cement.

Avaliação da construção e operação de técnicas compensatórias de drenagem urbana: o transporte de finos, a capacidade de infiltração, a taxa de infiltração real do solo e a permeabilidade da manta geotêxtil

This work aimed to evaluate some aspects of construction and operation of full-scale best management practices and their effects on infiltration loading rate. Two systems were studied: i) filter-swale-trench (FST) and ii) infiltration well (IW). In these units, field essays and full-scale measurements were made in order to assess the soil permeability. Further, the fine particles transportation and the geotextile blanket permeability were determined before and after operation. The results pointed out that there were soil transportation to inside the FST and IW systems, despite of the installed protections, and that these material were responsible for diminishing the geotextile (reduction from 30 to 90% for FST and 40 to 70% for IW) and the full-scale infiltration loading rates (varying from 4,7 x 10-6 to 10-5 m.s-1).