Formando lotes uniformes de reprodutores múltiplos e usando-os em acasalamentos dirigidos, em populações Nelore

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estimativas de efeitos genotípicos sobre os desempenhos pré e pós-desmama de populações Hereford x Nelore

Objetivou-se obter estimativas de efeitos genéticos aditivos e não-aditivos para as características pré e pós-desmama de animais Hereford x Nelore por meio de análises de regressão linear múltipla, com e sem o uso da técnica de regressão de cumeeira. Avaliaram-se as características ganho médio diário do nascimento à desmama, conformação, precocidade e musculatura à desmama, ganho médio diário da desmama ao sobreano, conformação, precocidade e musculatura ao sobreano e perímetro escrotal ajustado para idade e para idade e peso. Os resultados obtidos sem o uso da técnica indicaram valores acentuados dos fatores de inflação da variância. Para melhor interpretar os efeitos estimados, foram preditos os desempenhos de cinco gerações na formação do Braford ½ em relação à raça Hereford, partindo de vacas da raça Nelore. Os animais da geração F1 apresentaram alto desempenho, em razão do benefício máximo da heterose direta e do efeito aditivo materno. A manifestação completa da epistasia direta reduziu significativamente os desempenhos dos animais da geração F2. Para as características de desmama, os animais da geração F3 mostraram desempenhos menores, em virtude do efeito epistático materno máximo, uma vez que suas mães eram da geração F2. Os valores destas características estabilizaram na geração F4, próximos aos valores apresentados pela raça Nelore. Os desempenhos das gerações F3 e F4 para as características pós-desmama e os valores das estabilizações foram próximos ou superiores aos obtidos na geração F2.

Formação de populações-bases de aves para corte. I. Avaliação de melhoramento genético disponível no mercado

The present work aimed at studying the effect of five doses of zinc (0, 1.0, 2.0, 3.0 and 4.0 ppm) in three levels of soil correction. A Dark-Red Latosol, clay texture, oryginally recovered by cerrado vegetation was used. Soybean was used as test plant, cultivated in green-house conditions. The zinc dose affected dry matter production of aerial parts and grain only in the treatments that received the corrective. The concentration of the element in the soil increased linearly with the dose application, and the critical levels in soil (90% of the maximal yield) were between 0.27 and 0.37 ppm of zinc in the treatments with the equivalent to 2.0 t/ha and 3.2 t/ha of lime, respectively.

Crescimento e produtividade da mamona de porte baixo em diferentes espaçamentos e populações de plantas

It is necessary to establish adequate row spacings and plant populations for recently developed short height castor (Ricinus communis L) cultivars. This work aimed to evaluate the growth, yield components, and yield of short castor cultivar IAC 2028 as affected by row spacing and plant population, in summer cropping season. The experiments were carried out in 2007/08 and 2008/09 cropping seasons, in Botucatu County, São Paulo State, Brazil. A randomized complete block design in a split-plot scheme with four replications was used. Treatments assigned to plots were four row spacing (0.45, 0.60, 0.75, and 0.90m) and subplots were assigned to four levels of plant populations (25000, 40000, 55000, and 70000 plants/ha). The increase of plant population decreased plants survival rate, shoot dry weight, stem diameter, number of racemes per plant, number of fruits per raceme, and 100-grains weight. In higher plant populations, the best arrangement of plants, provided by the narrower row spacing (0.45m), promoted higher grain and oil yields. Increasing plant population increased grain and oil yields only when was used the narrower row spacing.

Efeito de aldicarb em populações de pulgão em artrópodes benéficos e na fisiologia do algodoeiro

In order to study the efficiency of aldicarb against aphids, beneficial arthropods, and on cotton physiology, a trial was made at Jaboticabal, SP, Brazil, in 1992/93. The experimental design was a complete randomized block with seven replications and three treatments: 1) aldicarb 750 g of a.i./ha; 2) aldicarb 1050 g of a.i./ha; and 3) control. The results showed that aldicarb was effective in the control of aphids. Aldicarb applied in the furrow significantly increased boll number per plant, yield, and precociousness. The beneficial arthropods were not affected.

Adubação fosfatada, produção e qualidade de sementes de populações de amendoim

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Diagnóstico laboratorial de hemoglobinopatias em populações diferenciadas

The inherited haemoglobinopathies are a heterogeneous group of recessive disorders that include the thalassaemias and sickle cell disease. Nearly a thousand mutant alleles have now been characterized. The mutations are regionally specific and in most cases the geographical and ethnic distribution shave been determined providing the foundation for a program of control through screening, genetic counseling and prenatal diagnosis. The diagnosis of hemoglobinopathies requires care for the methodologies applied and the population group which will be evaluated. The information about the abnormal hemoglobin, the medical and psychological aspects and genetic counseling of the carriers and their families are goals of great importance for the success of preventive programs in this area. Aiming to evaluate the laboratory methods for hemoglobinopathy screening and their use in clinical laboratories, we have compared abnormal hemoglobins incidence in the different population groups: blood donors, anemia carriers, newborn and students. The laboratory methods applied involved eletrophoretic proceedings, cytological and biochemical analysis. Within the period from September 1999 through January 2000, we analyzed 524 individuals with varied types of abnormal hemoglobins. Among blood donors, we diagnosed two sickle cell carriers, which suggest the necessity for better care in the process of selection of blood donor candidates. The current interest in the medical and social aspects of sickle cell anemia has resulted in a great increase in methodology research leading to the development of sickle cell screening techniques.

Estrutura genética de populações de Trichilia pallida Swartz (Meliaceae) por marcadores RAPD

Trichilia pallida Swartz is an Atlantic Forest shady climax tree of Meliaceae family that presents insecticide properties against chewing insects like as some family trees, making it interesting for forestry uses. Forty plants of Oito Pontas Farm population were collected in Bofete County, Santa Genebra Ecological Station in Campinas County, and Caetetus Ecological Station in Gélia County, all in the Sao Paulo State, Brazil. Leaf DNA analysis was used by RAPD method, that showed 10 highly polymorphic primers, with 72 dominant markers, used to estimate genetic diversity within and among populations. The polymorphism within populations varied from 90.3 to 97.2%, and the effective allele number varied from 1.46 ± 0.33 to 1.57 ± 0.33, while the average of genetic differentiation of populations varied from 0.27 ± 0.18 to 0, 33 ± 0.15. The gene diversity in the total population (H T) was 0.334 ± 0.02, while the average gene diversity within populations (H s) was 0.292 ± 0.017, and the coefficient of gene differentiation (G ST) was 0.125, Bofete and Campinas populations had the smallest Nei's genetic distance (0.049) and the distances of both with Gália were 0.117 and 0.107, respectively.

Ganho genético por diferentes critérios de seleção em populações segregantes de soja

In breeding programs, the complexity of economically outstanding characters, most of which highly influenced by the environment, makes selection difficult. Using genetic parameters, like heritability and gain with selection, it is possible to identify superior genotypes in early generations. Therefore, the aim of the present research was to compare different selection criteria by the estimated gains and the selected progenies, determining the superior and more similar methods. Augmented block design was used with three additional checks. Twelve hundred genotypes were evaluated. The most expressive expected gains were obtained with direct selection. However, the indices have shown to be more appropriate for selecting superior progenies because of their higher total gains, distributed among all the evaluated characters. The Sum of Ranks Index allowed the highest gains in most of the situations analysed.

Sistema de reprodução em pequenas populações fragmentadas e em árvores isoladas de Hymenaea stigonocarpa

Six microsatellite loci were used to quantify the mating system of two small fragmented populations (Selviria - SEL and Aparecida do Tabuado - APT, Mato Grosso do Sul State) and isolated trees in pastures, of the bat-pollinated tropical tree Hymenaea stignocarpa, growing in the Center-west region of Brazil. In SEL population, seeds were collected from 11 mother-trees; in APT, from three trees and, in the case of isolated trees, from six individuals growing at least 500 m apart in pastures. To investigate if there are differences on mating system between trees in populations and isolated trees, trees from populations were pooled as a group and, likewise, the isolated trees were pooled to another group. The outcrossing rate was higher in the populations (t̂ m= 0.873) than in isolated trees (t̂ m=0.857), but the difference was not significant. Significant and high differences between multi-locus and single-locus outcrossing rate were detected in populations (t̂ m- t̂ s=0.301, P<0.05) and isolated trees (t̂ m- t̂ s=0.276, P<0.05), suggesting mating between relatives. Higher paternity correlation was observed in trees from population (r̂ p=0.636) than in isolated trees (r̂ p=0.377), indicating the occurrence of some correlated matings and that part of offspring are full-sibs. It was not observed increased in self-fertilization rate in isolated trees in pastures. In general terms, the unique observed difference in mating system between populations and isolate trees was the high rate of correlated matings in trees from populations, due probably to the small distance among coespecifics and the pollinator behavior, visiting near trees.

Variação genética para compostos bioquímicos em sementes de duas populações naturais de Genipa americana L.: 1-análises individuais e univariada

Brazil is one of the world's richest countries in forest biodiversity. This has a great importance for humanity for sheltering essential scientific potential for the improvement in the quality of living beings. It also contributes to the preservation of natural resources and the environment. Among Brazil's forest species, jenipapo (Genipa americana L.) is commonly found in riparian vegetation. The objective of this work was to quantify the genetic variability of biochemical traits of seeds, as a support for in situ and ex situ conservation of natural populations of G. americana L. Two populations of the species were studied: one from Ilha Solteira, SP region (ISA) and other from Mogi Guaçu, SP (MOG). In the ISA population, seeds were collected from 30 trees, and in the MOG population, from 22 trees. These seeds were analyzed for chemical composition: content of proteins, carbohydrates, lipids and starch. The two populations of G. americana studied had a great genetic variation for these traits. The population ISA had an additive and phenotypic correlation, which was negative and high for starch with lipid; and positive and medium, for lipid with prolamine, as well as for globulin with prolamine. The population MOG presented additive and phenotypic correlations: positive and medium for gluteline with globulin. In these populations, narrow sense heritability estimates of progenies were moderate (0.69 for carbohydrates in the population ISA) to high (0.81 to 0.99 for the other traits in the two populations), indicating that much progress can be expected with selection strategies.

Estratégias de seleção dentro de progênies em duas populações de myracrodruon urundeuva Fr. All.

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Sistema de reprodução, parentesco e tamanho efetivo em sementes de polinização livre de populações fragmentadas de Copaifera langsdorffii Desf. por análise de locos microssatélites

Anthropogenic changes in the reproductive population density can affect the mating system and result in an increase in selfing and correlated matings. This study investigated the mating system in small fragmented populations of the insect pollinated tropical tree species Copaifera langsdorffii, using microsatellite loci and the mixed and correlated mating models. Open-pollinated seeds were collected from 15 seed-trees located in a small forest fragment (4.8 ha), denominated Bosque and from 14 other seed-trees located in other small forest fragments of the north-western region of São Paulo State. No significant differences were observed between the seed-trees from Bosque (tm=0.933±0.028) and other fragments (tm=0.971±0.032), although these estimates were significantly different from 1.0, suggesting that selfing was occurring. Differences between multilocus and unilocus outcrossing rate were significantly high in both seed-trees of Bosque (tm -ts=0.478±0.05) and other forest fragments (tm -ts=0.475±0.018), suggesting a spatial genetic structure in those stands. The results also showed high rates of correlated mating in the samples, indicating that a good part of the offspring were full-sibs. As a consequence of selfing, mating among relatives and correlated matings, the coancestry within families was equally high in the seed-trees of Bosque ((Θ=0.237) and in the seed-trees of the other forest fragments (Θ=0.241) and the effective population size was lower than expected in panmitic populations (Ne<4). The results were discussed, focusing on the sample size of seed-trees to collect seeds for genetic conservation and enviromental reforestation.

Sistemas de preparo do solo e populações de plantas em espaçamento reduzido: comportamento de cultivares de milho (Zea mays L.)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Interações interespecíficas e comportamentais de moscas-varejeiras: abordagens ecológicas e médico-legais envolvendo populações naturais

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)