Helminth parasites of hypsiboas prasinus (Anura: Hylidae) from two atlantic forest fragments, São Paulo State, Brazil

Seventy-seven males of Hypsiboas prasinus from 2 Atlantic forest fragments in the municipalities of Botucatu and Jundiaí, São Paulo State, Brazil, were examined for endoparasites. The frogs were captured in summer (January until March) and winter (July/August) of 2008 and 2009. Thirty-three males (75) from Botucatu were infected with Rhabdias cf. fuelleborni, cosmocerciid nematodes, and Cylindrotaenia americana. Twenty-five tree frogs (78.5) from Jundiaí were infected by Rhabdias cf. fuelleborni, Physaloptera sp., and cosmocerciid nematodes. Only cosmocerciid nematodes presented a statistically significance difference in prevalence (z 4.345; P < 0.001) and mean abundance (t 562.0; P < 0.001) between Botucatu and Jundiaí during the winter. Also, the cosmocerciids exhibited higher mean abundance (t 196.0; P 0.034) in winter when compared with summer at the Jundiaí site. Moreover, to our knowledge, this is the first report of C. americana in the Brazilian Hylidae. This study presents 4 new records of nematodes in H. prasinus. © 2012 American Society of Parasitologists.

Lanfrediella amphicirrus gen. nov. sp. nov. Nematotaeniidae (Cestoda: Cyclophyllidea), a tapeworm parasite of Rhinella marina (Linnaeus, 1758) (Amphibia: Bufonidae)

The family Nematotaeniidae, tapeworms commonly found in the small intestines of amphibians and reptiles, includes 27 recognised species distributed among four genera: Bitegmen Jones, Cylindrotaenia Jewell, Distoichometra Dickey and Nematotaenia Lühe. The taxonomy of these cestodes is poorly defined, due in part to the difficulties of observing many anatomical traits. This study presents and describes a new genus and species of nematotaeniid parasite found in cane toads (Rhinella marina) from eastern Brazilian Amazonia. The cestodes were collected during the necropsy of 20 hosts captured in the urban area of Belém, Pará. The specimens were fixed and processed for light microscopy, scanning electron microscopy (SEM) and three-dimensional (3D) reconstruction. Samples were also collected for molecular analyses. The specimens presented a cylindrical body, two testes and paruterine organs. However, they could not be allocated to any of the four existing nematotaeniid genera due to the presence of two each of dorsal compact medullary testes, cirri, cirrus pouches, genital pores, ovaries and vitelline glands per mature segment. Lanfrediella amphicirrus gen. nov. sp. nov. is the first nematotaeniid studied using Historesin analysis, SEM and 3D reconstruction, and it is the second taxon for which molecular data have been deposited in GenBank.

Helmintofauna associativa à anfíbios da Ilha Anchieta, litoral norte do estado de São Paulo, Brasil

Pós-graduação em Ciências Biológicas (Zoologia) - IBB

Helmintofauna associada a uma guilda de anfíbios do pantanal Sul -Matogrossense

A helmintofauna tem papel central no ecossistema, pois afeta a ecologia, evolução, crescimento e regulação da população de hospedeiros e, consequentemente, toda a biodiversidade. Neste estudo foram selecionados anuros pertencentes a uma guilda, de acordo com o modo reprodutivo e sítio de vocalização, e foi estudada a supracomunidade de parasitas associadas a estes anfíbios. Em janeiro de 2009 foram coletados 60 anuros no Pantanal Sul- Matogrossense, sub-região Miranda-Abobral no município de Corumbá, MS. As espécies de anuros coletadas foram Rhinella granulosa, R. schineideri, Pseudis paradoxa, P. limellum, Pleurodema fuscomaculata e Physalaemus albonotatus. Os anuros foram necropsiados e analisados quanto à presença de helmintos. Dezesseis espécies de helmintos foram identificadas em 50 indivíduos hospedeiros (prevalência total = 83,3%). Dentre os parasitas foram encontradas três espécies de trematódeos (Catadiscus marinholutzi, Catadiscus propinquus e Glypthelmins palmipedis), uma espécie de cestoda, Cylindrotaenia americana, dez espécies de nematóides (Aplectana sp., Contracecum sp.,Cosmocerca podicipinus, Cosmocercidae, Falcaustra mascula, Physaloptera sp., Ochoterenella sp., Oswaldocruzia subauricularis, Physalopteroides venancioi sp. e Rhabdias sp.) e cistos de acantocéfalo. Os trematódeos foram encontrados apenas em P. paradoxa e P. albonotatus, enquanto C. americana foi encontrada apenas em P. fuscomaculata. As espécies Aplectana sp. e Falcaustra máscula foi encontrada apenas em R. schineideri. O único indivíduo encontrado em P. limellum foi da espécie Physalopteroides venancoi. As suprapopulações de parasitas se mostraram mais agregadas em R. granulosa e P. paradoxa, onde seus hábitos de vida e hábitos alimentares podem influenciar na comunidade parasitária. Logo o tamanho do hospedeiro pode influenciar na sua ...(Resumo completo, clicar acesso eletrônico abaixo)

Sazonalidade climática e os efeitos na helmintofauna parasita de Dendropsophus nanus (Anura: Hylidae) da RPPN Foz do rio Aguapeí, município de Castilho, São Paulo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

HELMINTH PARASITES OF HYPSIBOAS PRASINUS (ANURA: HYLIDAE) FROM TWO ATLANTIC FOREST FRAGMENTS, SAO PAULO STATE, BRAZIL

Seventy-seven males of Hypsiboas prasinus from 2 Atlantic forest fragments in the municipalities of Botucatu and Jundiai, sao Paulo State, Brazil, were examined for endoparasites. The frogs were captured in summer (January until March) and winter (July/August) of 2008 and 2009. Thirty-three males (75%) from Botucatu were infected with Rhabdias cf. fuelleborni, cosmocerciid nematodes, and Cylindrotaenia americana. Twenty-five tree frogs (78.5%) from Jundiai were infected by Rhabdias cf. fuelleborni, Physaloptera sp., and cosmocerciid nematodes. Only cosmocerciid nematodes presented a statistically significance difference in prevalence (z = 4.345; P < 0.001) and mean abundance (t = 562.0; P < 0.001) between Botucatu and Jundiai during the winter. Also, the cosmocerciids exhibited higher mean abundance (t = 196.0; P = 0.034) in winter when compared with summer at the Jundiai site. Moreover, to our knowledge, this is the first report of C. americana in the Brazilian Hylidae. This study presents 4 new records of nematodes in H. prasinus.