Relação da taxa de gestação com sêmen bovino congelado e testes de avaliação espermática in vitro

Avaliou-se a relação entre os testes complementares (teste hiposmótico, teste de termorresistência lento e teste de reação acrossômica) e os testes de avaliações convencionais (aspectos físicos e morfológicos) de sêmen bovino congelado/descongelado e os índices de prenhez. Os valores médios da motilidade espermática progressiva retilínea avaliados pelo teste de termorresistência foram de 53,48 (pós-descongelamento), 43,69 (60 minutos), 35,88 (120 minutos) e 33,04% (180 minutos) e a porcentagem de células reativas ao teste hiposmótico foi de 37,89%. Correlação positiva e de média intensidade foi encontrada para a motilidade espermática progressiva retilínea pós-descongelamento e o teste hiposmótico (0,21). Entretanto, a correlação da motilidade aos 180 minutos com o teste hiposmótico foi alta (0,64). A porcentagem de células que tiveram acrossoma reagido pós-descongelamento foi de 9,85%, apresentando correlações negativas de média e alta intensidade (-0,25 e -0,46, respectivamente) com a motilidade espermática progressiva retilínea pós-descongelamento e após 3 horas de incubação. Não houve correlação dos testes complementares e da motilidade pós-descongelamento com a taxa de gestação. Nenhum parâmetro considerado isoladamente serviu para avaliar a capacidade fertilizante do sêmen congelado/descongelado.

Influência da estação do ano, estádio de lactação e da hora da ordenha sobre o número de células somáticas do leite bovino

From January to October of 1995, somatic cell countings were accomplished in 2,218 milk samples collected from 67 quarters of 17 lactating cows at the initial, middle and final stages of lactation, in the morning and evening milkings. The highest means of cell countings were observed among the milk samples collected at the Final stage of lactation 15.652cell/ml), in the winter (5.358cell/ml) and the afternoon milking (5.199cell/ml). The differences observed amongst the cell countings in samples obtained at the different stages of lactation and the morning and afternoon milkings were statistically significant (P < 0.0001). In contrast differences observed amongst the seasons of the year, showed to be non-significant at the level of 5% probability. Higher occurrence of samples with cell countings superior to 500,000cell/ml was verified at the final stage of lactation (34.9%), in the winter (23.6%) and the afternoon milking (21.3%). These findings show the influence of physiologic and management factors (stage of Lactation and milking time) on milk cell concentrations.

Digestilidade in vitro do bagaço de cana-de-açúcar hidrolisado, submetido a diferentes tempos de estocagem e de adaptação ao bovino doador de fluido ruminal

An experiment was conducted to study the in vitro digestibility of dry matter (IVDMD) and gross energy (IVGED) of hydrolyzed sugarcane bagasse with different storage times and two periods of adaptation of the bovine to the diet. A completely randomized design with four treatments and five replications was used. Treatments A,B,C, and D which corresponded to the times of 0, 15, 30, and 45 days of storage time period of the bagasse, respectively. Each treatment was studied at 7 and 45 after adaptation of the bovine ruminal donor to the diet. After 30 days storage, the bagasse IVDMD was inpaired for both 7 and 45 days of adaptation. Averages values of 25.8; 25.9; 23.1 and 23.3% were obtained for IVDMD with seven days of adaptation, and 25.04; 21.6; 12.5 and 11.4% with 45 days of adaptation for storage periods of 0; 15; 30 and 45 days, respectively. For IVGED, the averages obtained were 33.4; 27.8; 30.9; and 27.2% with seven days, and 30; 26.6; 20.6 and 14.5% with 45 days of adaptation for storage periods of 0, 15, 30 and 45 days, respectively.

Detecção de herpesvírus bovino 5 (BoHV-5) em bovinos do Sudeste Brasileiro

This paper reports the detection of bovine herpesvirus type 5 (BoHV-5) by a specific nested PCR assay. Samples were collected from the central nervous system (CNS) of cattle from Minas Gerais and São Paulo States, Brazil. All animals died presenting neurological symptoms. Nineteen frozen CNS samples analyzed had been previously tested by fluorescence antibody test for rabies virus and showed negative results. Three paraffin-embedded brain tissue samples were examined by histopatology and the observed alterations suggested nonsuppurative meningoencephalitis. BoHV-5 was detected in five (22.7%) among 22 tested samples. The occurrence of BoHV-5 infection is reported in the Southeast region of Brazil, indicating that epidemiological studies should be carried out.

Herpes Virus Bovino tipo 1 (BoHV-1) como posible causa de encefalitis en bovinos de la región del Magdalena Medio Colombiano. Estudio serológico y análisis epidemiológico

The bovine Herpesvirus type 1 and type 5 (BoHV-1 and BoHV-5), causing diseases and significant economic losses in farms of worldwide. Both affect the nervous system of cattle, although BoHV-5 has been the most associated with this type of pathogenesis. Given the death of animals with nervous symptoms and negative diagnoses for rabies virus in the area of study, this research focused on the detection of positive reactors to bovine herpes virus serum neutralization. We collected 518 blood samples from animals without Herpesvirus vaccine, in the municipalities of Caparrapi, Cimitarra, Honda and Victoria, in the Middle Magdalena River Region. In addition, epidemiological information useful to discuss neurological disease was collected through primary and secondary sources. For the analysis of data was used chi-square test by identification of relationship between evidence of viral infection and the variables recorded. The results revealed that 286 cases were positive for Herpesvirus infection, corresponding to a prevalence of 55.5%, however, there was no statistical relationship (p < 0.05) between the presence of antibodies and the variables analyzed. In conclusion, some cases of neurological disease in cattle in this region could be due to infection with herpes viruses. We discussed about the presence of BoHV-1 and BoHV-5 in the ambient, diagnosis and monitoring plans, as well as economic losses, which may cause in herds in this area.

Prevalência de reagentes ao teste de tuberculinização no rebanho bovino leiteiro da região de São Carlos - SP

Tuberculosis (TB), an infectious disease readily transmitted between animals and humans, is an great threat to public and animal health a like and has been declared an international emergency. According to the WHO, animal TB is a source of much concern, especially in the poorer countries where there is little awareness of the problem. Thus, improvements in veterinary health are essential, with regard to infection by Mycobacterium bovis, particularly in areas where the public is at risk. The prevalence of infected animals in Brazil is about 1%, whereas, in the dairy cattle region of São Carlos, the average proportion of positive tests was found to be 3. 1% from 1987 to 1996. The current research was carried out to investigate the prevalence, in the region, of dairy cattle reacting positively to the tuberculin test, from 1997 to the first half of 2001. The results were obtained by searching through the data collected by the Federal Inspection Authority, and they indicated an average prevalence of 1.3% over the period, similar to the official national average. It was concluded that bovine TB continues to be present in the region, posing a potential threat of infection to the human population.

Peso de abate de machos não-castrados para produção do bovino jovem. 1. Desempenho em confinamento e custos de produção

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Peso de abate de machos não-castrados para produção do bovino jovem. 2. Peso, Idade e caracteristicas da carcaça

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Detecção de Leptospira pomona em sêmen bovino por eletroforese capilar fluorescente

This study was performed in order to evaluate the detection limit of PCR with fluorescent capillary electrophoresis for Leptospira pomona diagnosis in bovine semen. Negative bovine semen samples were artificially contaminated with Leptospira pomona (10 0 to 10 7 bacteria/ ml) and DNA was extracted by phenol/chloroform protocol. DNA fragments visualization was done by three electrophoresis methods: under UV light in 2 % agarose gel, silver staining 8% polyacrylamide gel and fluorescent capillary electrophoresis. The detection limit of capillary electrophoresis for Leptospira pomona was 10 2bacteria/ml. Under UV light, in 2 % agarose gel, the detection limit was of 10 4 bacteria/ ml while for silver stained 8 % polyacrylamide gel it was 10 2 bacteria/ ml. PCR with fluorescent capillary electrophoresis is an efficient and rapid diagnostic test for DNA detection of Leptospira in bovine semen and this can be an important tool for herd and semen sanitary control in artificial insemination centers.

Polimorfismo da beta-lactoglobulina não afeta as caracterí sticas físico-químicas e a estabilidade do leite bovino

The objective of this study was to evaluate the effect of beta-lactoglobulin polymorphism, breed and seasonality on physico-chemical characteristics and stability of bovine milk. Six Holstein-Zebu (H-Z) and five Holstein dairy herds were selected, of which 660 Holstein and 293 crossbred Holstein-Zebu milk and blood samples were collected, adding up 953 samples, distributed in two collecting periods at rainy and two at dry seasons. Each milk sample was analyzed for titratable acidity, pH, freezing point and milk ethanol stability, at the following ethanol concentrations: 70, 76, 80 and 84GL. Individual blood samples were submitted to polymerase chain reaction (PCR) for the determination of beta-lactoglobulin polymorphism. No effect of beta-lactoglobulin polymorphism was observed in physico-chemical characteristics of milk, in both studied breed. Breed effect for Holstein and H-Z, respectively, was observed on titratable acidity (16.16 and 17.07°D, while effect of seasonality (for rainy and dry seasons, respectively) was also observed on freezing point (-0.5411 and -0.5376°H). Effects of breed and seasonality on milk stability were observed (Holstein-Zebu milk was less stable on dry season), however, no effect of beta-lactoglobulin on milk stability was observed.

Descripción inmunohistoquímica de la encefalitis en bovinos brasileños naturalmente infectados con el herpesvirus bovino tipo 5 (HVB-5)

Twelve cases of viral meningoencephalitis in Brazilian cattle were submitted to immunohistochemical analysis for inflammatory response description. All the cases showed severe neurological signs followed by death. Mild to moderate histological inflammatory changes in the brain and cerebellum characterized the neurological infection showing meningitis, mononuclear perivascular cuffing, gliosis, haemorrhage, and macrophages (gitter cells) accompanying great areas of malacia. None of the cases showed intranuclear inclusion bodies. However, in five of them it was possible to isolate the BoHV-5. In order to collect data to allow the description of the inflammatory response in these cases, brain samples from all of the cases were analyzed by immunohistochemistry using polyclonal antibodies against CD3 to detect T cells, and against GFAP to detect astrocytes. On the other hand, monoclonal antibodies were used against BLA to detect B cells and, against MAC 387 to detect macrophages. The results indicate different degrees of prominent astrocytic response, and at the same time, T lymphocytes constituted a high percentage of the mononuclear cells which characterized the inflammatory response.

Estudo da perda da tensao do enxerto de tendão calcâneo bovino

Lesion of the anterior cruciate ligament (ACL) in the knee is a problem that affects animals as well as humans and may evolve with joint instability that is often symptomatic and incapacitating. The main option for ligament substitution is the autologous tendon graft. The graft undergoes tensioning during ACL reconstructive surgery to reestablish the normal laxity of the ACL- deficient knee. Although graft tensioning plays a fundamental role in postoperative clinical evolution, ideal tensioning levels have not been established in the literature. Therefore, graft elongation that may occur over time is still one of the main reasons for ligament reconstruction failure. In this study, ten bovine calcaneus tendons underwent two successive assays of physiological tensioning for a maximum deformation of 2.5% of the initial tendon length, maintained for 600s with force (N) values recorded at zero time (initial), 300s and 600s. At the end of the first assay, the tendon returned to its initial length and was maintained at rest for 300s and then the next tensioning assay was initiated, repeating the previous procedure. Statistical analysis revealed that tendon elongation is more pronounced during the initial 300s, reflecting a more accentuated decline in the tension values of the graft. Therefore, 300s after tensioning and fixing the graft, the surgeon can assess with more precision if the tensioning level was adequate.

Esterco bovino para o desenvolvimento inicial de plantas provenientes de quatro matrizes de Corymbia citriodora

This work aimed to evaluate the effect of cattle manure levels in the phase of implementation Corymbia citriodora (Hook.) K. D. Hill & L. A. S. Johnson (Eucalyptus citriodora) seedlings in field, produced from seeds collected from four mother trees. The experiment was carried out in green house, in pots filled with 5 dm 3 of Oxisol. Five cattle manure levels were applied 0, 10, 20, 30, and 40 t ha -1 (0, 25, 50, 75, and 100 g pot -1). All treatments received lime levels which were calculated to increase the base saturation degree to 50%. The soil fertility was evaluated after 30 days of soil incubation with manure and lime. The seeds were collected from mother trees called 2, 8, 20 and 29. Sowing was performed directly in rigid plastic containers of 50 cm 3 and seedlings were transplanted when they were around 17 cm height. The experimental design was entirely randomized, in a factorial 5 × 4 (five doses of cattle manure and seeds of four mother trees) scheme and four repetitions. Each parcel was a pot with two plants. At 90 days the height, foliar area, stalk diameter, shoot and root dry matter. The plants responded positively to application of manure, but differently for each evaluated growth characteristic linearly or quadratically. The manure levels had linear effects on growth characteristics and dry matter production of the plants from mother trees 2 and 20 moreover these plants require more cattle manure levels than ones from mother trees 8 and 29. The cattle manure promoted the best development of plants from mother trees 8 and 29, in relationship with dry matter production of shoot components was approximately 27 t ha-1, equivalent to 67.5 g pot.