Análise filogenética de Phytophthora capsici Leonian do estado de São Paulo baseada na seqüência de nucleotídeos da região ITS-5.8S rDNA

Phylogenetic analysis through morphological and cultural traits is considered inconsistent and hard to be scientifically accepted once there is no way to establish the direction of evolution on morphological traits. Molecular markers are suitable for phylogenetic analysis. The sequencing of ITS1 and ITS2 (internal transcribed spacers) regions and of 5.8S gene from ribosomal DNA were used to estimate the genetic variation and distance of 10 Phytophthora capsici isolates. The amount of genetic variation amongst isolated P. capsici from distinct regions of São Paulo State was 0.1 to 1.6 and 0.1 to 1.1% at ITS1 and ITS2, respectively, and a phylogenetic distance of about 78.5% between P. capsici and Phytophthora spp. was observed.

Valor preditivo do potencial de implantação embrionário pela análise do perfil químico de meios de cultivo por espectrometria de massas (ESI-Q-ToF)

Objective: This case-control study analyzed mass spectrometry fingerprinting patterns of culture media samples used for embryo culture to predict embryo implantation. Methods: The culture medium harvested after embryo transfer of 22 embryos from 13 patients was used for the experiments. After embryo transfer, the remaining culture media were collected and samples were split in positive (n=8) and negative (n=14) implantation groups according to implantation outcomes (100% or 0% of implantation). Samples were individually diluted and injected directly to the Electrospray ionization (ESI) MS coupled to a Quadrupole Time-of-flight MS (Q-ToF-MS).Ions relative intensities of each spectrum were considered. Data analysis was conducted in MatLab 7.0 version using Partial Least Squares - Discriminant Analysis toolbox. Results: There were 3027 observed ions at 100% and 0% implantation groups by ESI-Q-ToF-MS. The statistical model could categorize the samples in two clusters, based on their positive and negative implantation outcomes. Less intense ions present in the mass spectra with statistical significance have contributed to the major differences to group distinction. Conclusions: Positive and negative implantation embryos showed a specific biochemical pattern present in culture media, which could be detected as a fast, simple and non-invasive way. This biochemical profile could help the selection of the most viable embryo, improving single embryo transfer and thus eliminating the risk and undesirable outcomes of multiple pregnancies. © Todos os direitos reservados a SBRA - Sociedade Brasileira de Reprodução Assistida.

Sistema de reprodução, parentesco e tamanho efetivo em sementes de polinização livre de populações fragmentadas de Copaifera langsdorffii Desf. por análise de locos microssatélites

Anthropogenic changes in the reproductive population density can affect the mating system and result in an increase in selfing and correlated matings. This study investigated the mating system in small fragmented populations of the insect pollinated tropical tree species Copaifera langsdorffii, using microsatellite loci and the mixed and correlated mating models. Open-pollinated seeds were collected from 15 seed-trees located in a small forest fragment (4.8 ha), denominated Bosque and from 14 other seed-trees located in other small forest fragments of the north-western region of São Paulo State. No significant differences were observed between the seed-trees from Bosque (tm=0.933±0.028) and other fragments (tm=0.971±0.032), although these estimates were significantly different from 1.0, suggesting that selfing was occurring. Differences between multilocus and unilocus outcrossing rate were significantly high in both seed-trees of Bosque (tm -ts=0.478±0.05) and other forest fragments (tm -ts=0.475±0.018), suggesting a spatial genetic structure in those stands. The results also showed high rates of correlated mating in the samples, indicating that a good part of the offspring were full-sibs. As a consequence of selfing, mating among relatives and correlated matings, the coancestry within families was equally high in the seed-trees of Bosque ((Θ=0.237) and in the seed-trees of the other forest fragments (Θ=0.241) and the effective population size was lower than expected in panmitic populations (Ne<4). The results were discussed, focusing on the sample size of seed-trees to collect seeds for genetic conservation and enviromental reforestation.

Análise molecular em morcegos do gênero Artibeus Leach, 1821 (Chiroptera, Phyllostomidae) a partir de espécimes depositados em coleção científica

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Evidências moleculares dos padrões evolutivos e filogeográficos de populações de Astyanax paranae (Pisces : Characidae) com base em caracteres do DNA mitocondrial

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise citogenética clássica, molecular e ultraestrutural em escorpiões da família Buthidae: um enfoque evolutivo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise dos efeitos citotóxicos, genotóxicos e mutagênicos do inseticida malation, utilizando os sistemas teste de Allium cepa e células de mamíferos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise do imprinting e da expressão dos genes H19 e IGF2 em células de Sertoli humanas apos exposição in vitro ao 2,3,7,8-Tetraclorodibenzo-p-dioxina (TCDD)

Pós-graduação em Biologia Geral e Aplicada - IBB

Utlização do DNA mitocondrial no contexto forense brasileiro

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Identificação e análise de polimorfismos no gene que codifica a Lectina ligante de Manose (MBL) na espécie Canis lúpus familiaris

Pós-graduação em Biologia Geral e Aplicada - IBB

Caracterização funcional de duas proteínas de Neurospora crassa identificadas em complexos DNA-proteína

Pós-graduação em Biotecnologia - IQ

Análise prospectiva do padrão de metilação nos genes associados a doenças cardíacas SCN5A e MTR para aplicação na genética forense

Pós-graduação em Biotecnologia - IQ

Os estudos de Avery, Macleod e Mccarty e a idéia do DNA como responsável pela hereditariedade: interpretações historiográficas e apontamentos para o ensino de biologia

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Isolamento e caracterização de marcadores de DNA associados ao sexo e segmentos de DNA repetitivo em espécies do gênero Brycon (Characidae, Bryconinae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise de variações biológicas em indivíduos com gagueira desenvolvimental persistente familiar

Pós-graduação em Ciências Biológicas (Genética) - IBB