三维遗传信息与第三遗传密码

本文系对生物系统的一个重大科学问题由假说到求证所作的阶段性自主探索的报导和讨论。主要涉及 mRNA 与编码蛋白质之间是否存在三维结构的遗传关系和对破译"第三遗传密码"的探索。事实上,基于实验数据和结构模型,我们已经发现和找到多项证据来证实这种关系的存在,但还缺乏直接的关键生物学实验证据来证明"三维遗传信息"存在的正确性。目前,生物学实验已起步。

淋巴细胞免疫治疗原发性习惯性流产

对49例确诊为原发性习惯性流产(41.76%)中的29例采用丈夫肘静脉血40ml分离淋巴细胞行主动免疫治疗(一般妊娠前治疗两次,妊娠后治疗两次),现有17例妊娠,其中11例已分娩;5例在中期妊娠;1例失败。妊娠成功率为94.12%(16/17)。另20例未经主动免疫治疗作为对照组,妊娠成功率为27.78%(5/18),两组相比差异有显著性(P<0.05),对部分病例进行了治疗前免疫状态检测,包括微淋巴细胞毒抗体阴性,杀伤淋巴细胞低下改变的检测。结果提示丈夫淋巴细胞主动免疫法的有效性及可提高患者免疫反应性,有较可靠的临床效果及应用价值。

藏酋猴(Macaca thibetana)与猕猴(Macaca mulatta)长骨生长的比较研究

对15具不同年龄组的藏酋猴长骨的形态功能进行统计学处理和与猕猴比较分 析表明, 藏酋猴具有较长的躯干骨, 相对较短的前后肢和桡骨, 较高的肢间指 数(94.7)和低的臂指数(94.1)。在发育过程中, 其肢骨、体重和躯干骨的增长 速率均大于猕猴, 前后肢增长率相近。猕猴后肢的增长明显大于前肢, 具相对较 短的前肢。藏酋猴更适应于地栖生活。图3表5参15

从动物学期刊文献量预测中国动物学研究发展趋势

我国动物科学过去几十年内在追赶世界先进国家的努力中取得了令人瞩目的成就，但据预测每年文献量以0.1%的速度递减，其原因仍存在许多不利因素．限制和制约着动物学发展. 教育落后于时代要求：首先是中学生物学教育问题，在高考中取消生物学考试，这本身就极大地影响青少年的生物科学素质，而且对今后大学生、研究生的生源和质量，以及新一代动物科学研究人才的涌现均产生深远的不利影响。

树鼩细胞周期蛋白T1 cDNA的克隆和序列分析

树鼩细胞不能感染 HIV-1, 但支持 HIV-1进入靶细胞后的转录, 可能是因为树鼩细胞周期蛋白T1(tsCycT1)能结合HIV-1的 Tat 蛋白. 通过设计引物, 用 RT-PCR 技术, 获得全长为2175bpts CycT1 基因的cD NA. 其核苷酸序列与人的 CycT1(hCycT1) 基因的 cDNA 有92.6%的相似性; 由此推导出的氨基酸序列有94.1%相似性. 其中, hCycT1 和 tsCycT1 氨基端的1～272个氨基酸的相似性高达98.8%, 氨基酸第261位点为半胱氨酸. 这些结果提示, tsCycT1 会和 HIV-1 的 Tat 结合, 形成高亲和的、锌依赖的复合物, 支持 HIV-1转录。

FEEDING AND VOCAL BEHAVIORS OF BLACK GIBBONS (HYLOBATES-CONCOLOR) IN YUNNAN - A PRELIMINARY-STUDY

Feeding and vocal behaviours of wild black gibbons (Hylobates concolor) were observed from 1987 to 1989 in south-western Yunnan, notably H. concolor jingdongensis at Mt. Wuliang (24-degrees 18-42'N, 100-degrees 30-50'E) in the early spring of 1989. 12 plant species were observed to have been eaten by the gibbons; these included tree species, lianas and epiphytes. Approximately 21 % of feeding time was devoted to eating fruits, 61 % to leaf buds and shoots, 7 % to flowers and 11 % to leaves. The gibbons preferred fruits to leaves even though they commonly ate leaves. In this study, the morning songs (duet and solo), reponsive (territorial) songs, alarm calls and communication calls were recorded. The gibbons sang their morning songs mainly in the early morning, with a single bout lasting more than 10 min on average. The singing of a group would trigger other groups, and all groups in an area tended to sing sequentially. The morning duet song bout was dominated by an adult male. The male emitted booms, aa notes, early multimodulated figures, intermediate multimodulated figures and codas, the latter occurring only in duets following the female's great call. The female uttered great calls and abortive great calls. The subadults or juveniles also took part in the morning songs. Lone males were heard to utter solos which lasted longer than the duets of the pairs.

Molecular docking and 3D-QSAR study of pyranmycin derivatives against 16S rRNA A site

To understand pharmacophore properties of pyranmycin derivatives and to design novel inhibitors of 16S rRNA A site, comparative molecular field analysis (CoMFA) approach was applied to analyze three-dimensional quantitative structure-activity relationship (3D-QSAR) of 17 compounds. AutoDock 3.0.5 program was employed to locate the orientations and conformations of the inhibitors interacting with 16S rRNA A site. The interaction mode was demonstrated in the aspects of inhibitor conformation, hydrogen bonding and electrostatic interaction. Similar binding conformations of these inhibitors and good correlations between the calculated binding free energies and experimental biological activities suggest that the binding conformations of these inhibitors derived from docking procedure were reasonable. Robust and predictive 3D-QSAR model was obtained by CoMFA with q(2) values of 0.723 and 0.993 for cross-validated and noncross-validated, respectively. The 3D-QSAR model built here will provide clear guidelines for novel inhibitors design based on the Pyranmycin derivatives against 16S rRNA A site. (c) 2005 Elsevier B.V. All rights reserved.

对磷酸氢钙(HG2636-94)检测方法的改进建议

本文对饲料级磷酸氢钙标准(HG2636-94)检测方法中钙含量的测定、氟含量的测定存在的问题进行了探讨,并提出了解决方法.利用分散剂消除磷酸根对钙测定的干扰,EDTA直接络合滴定钙,方法快速准确;控制适当的试科量以保证氟检测结果的准确度.

Analysis of mode quality factors and mode reflectivities for nanowire cavity by FDTD technique

The mode frequency and the quality factor of nanowire cavities are calculated from the intensity spectrum obtained by the finite-difference time-domain (FDTD) technique and the Pade approximation. In a free-standing nanowire cavity with dielectric constant epsilon = 6.0 and a length of 5 mu m, quality factors of 130, 159, and 151 are obtained for the HE11 modes with a wavelength around 375 nm, at cavity radius of 60, 75, and 90 nm, respectively. The corresponding quality factors reduce to 78, 94, and 86 for a nanowire cavity standing on a sapphire substrate with a refractive index of 1.8. The mode quality factors are also calculated for the TE01 and TM01 modes, and the mode reflectivities are calculated from the mode quality factors.