Renal C-type natriuretic peptide and natriuretic peptide receptor B mRNA expression are affected by water deprivation in the Spinifex Hopping mouse

This study investigated the effect of water deprivation on the expression of C-type natriuretic peptide (CNP) and natriuretic peptide receptor B (NPR-B) mRNA, and the ability of NPR-B to generate cGMP in the Spinifex Hopping mouse, Notomys alexis. This rodent is a native of central and western Australia that is well adapted to survive in arid environments. Initially, CNP and NPR-B cDNAs (partial for NPR-B) were cloned and sequenced, and were shown to have high homology with those of rat and mouse. RT-PCR analysis showed CNP mRNA expression in the kidney, proximal and distal colon and small intestine, whilst NPR-B mRNA expression was found in the kidney, proximal and distal colon and the atria. Using a semi-quantitative multiplex PCR technique, the expression of renal CNP and NPR-B mRNA was determined in 7- and 14-day water-deprived hopping mice, in parallel with control hopping mice (access to water). Water deprivation significantly decreased the relative levels of CNP and NPR-B mRNA expression in both the 7- and 14-day water-deprived hopping mice, when compared to control hopping mice. In contrast, the ability of CNP to stimulate cGMP production was significantly increased after 14 days of water deprivation. This study shows that alterations in the renal CNP/NPR-B system may be an important physiological adjustment when water is scarce.

The development of an efficient checkpointing facility exploiting operating systems services of the GENESIS cluster operating system

Recent research efforts of parallel processing on non-dedicated clusters have focused on high execution performance, parallelism management, transparent access to resources, and making clusters easy to use. However, as a collection of independent computers used by multiple users, clusters are susceptible to failure. This paper shows the development of a coordinated checkpointing facility for the GENESIS cluster operating system. This facility was developed by exploiting existing operating system services. High performance and low overheads are achieved by allowing the processes of a parallel application to continue executing during the creation of checkpoints, while maintaining low demands on cluster resources by using coordinated checkpointing.

Identification of an α-peptide in Haliotis rubra with homology to the Lymnaea α-CDCP spawning peptide

It is now understood that a combination of molluscan reproductive peptides are commonly cleaved from a large preprohormone and influence different aspects of spawning behavior. One type of reproductive peptide, known in Lymnaea stagnalis as [alpha]-CDCP, and in Aplysia californica as [alpha]-BCP, acts in egg laying via temperature-dependent autoinhibition or autoexcitation of neuronal cells. In our study, the expression of [alpha]-CDCP-like peptide in the blacklip abalone, Haliotis rubra, was identified by Western blots and immunocytochemistry, using an antiserum developed against [alpha]-CDCP. Western blots of total protein isolated from the central nervous system, cerebral and pleuropedal ganglia, as well as gonad and heptopancreas tissues of sexually mature adults, identified a protein of approximately 100 kDa as well as a range of smaller reactive peptides. This finding suggests that a reproductive [alpha]-peptide is probably synthesized from a single larger precursor protein. The larger peptides were also identified in Western blots of several abalone tissues, lmmunocytochemistry using the same antiserum showed the presence of immunoreactive axons in all the tissues studied, indicating synthesis or transport of products. The function of the abalone [alpha]-CDCP-like peptide is yet to be determined.

Natriuretic peptide guanylyl cyclase receptors in the kidney of the Japanese eel, Anguilla japonica

Natriuretic peptides are linked to osmoregulation, cardiovascular and volume regulation in fishes. The peptides bind to two guanylyl-cyclase-linked receptors, natriuretic peptide receptor-A (NPR-A) and NPR-B, to elicit their effects. Atrial natriuretic peptide (ANP) binds principally to NPR-A, whereas C-type natriuretic peptide (CNP) binds to NPR-B. The teleost kidney has an important role in the maintenance of fluid and electrolyte balance; therefore, the location of NPR-A and NPR-B in the kidney could provide insights into the functions of natriuretic peptides. This study used homologous, affinity purified, polyclonal antibodies to NPR-A and NPR-B to determine their location in the kidney of the Japanese eel, Anguilla japonica. Kidneys from freshwater and seawater acclimated animals were fixed overnight in 4% paraformaldehyde before being paraffin-embedded and immunostained. NPR-A immunoreactivity was found on the apical membrane of proximal tubule 1 and the vascular endothelium including the glomerular capillaries. In contrast, NPR-B immunoreactivity was located on the smooth muscle of blood vessels including the glomerular afferent and efferent arterioles, and on smooth muscle tissue surrounding the collecting ducts. No difference in the distribution of NPR-A and NPR-B was observed between freshwater and seawater kidneys. Immunoreactivity was not observed in any tissue in which the antibodies had been preabsorbed. In addition, there was no difference in NPR-A and NPR-B mRNA expression between freshwater-acclimated and seawater-acclimated eels. These results suggest that, although utilizing the same second messenger system, ANP and CNP act on different targets within the kidney and presumably elicit different effects.

Exploiting operating system services to efficiently checkpoint parallel applications in GENESIS

Recent research efforts of parallel processing on non-dedicated clusters have focused on high execution performance, parallelism management, transparent access to resources, and making clusters easy to use. However, as a collection of independent computers used by multiple users, clusters are susceptible to failure. This paper shows the development of a coordinated checkpointing facility for the GENESIS cluster operating system. This facility was developed by exploiting existing operating system services. High performance and low overheads are achieved by allowing the processes of a parallel application to continue executing during the creation of checkpoints, while maintaining low demands on cluster resources by using coordinated checkpointing.

Grid-based method for ranking images with multiple objects

One of the content-based image retrieval techniques is the shape-based technique, which allows users to ask for objects similar in shape to a query object. Sajjanhar and Lu proposed a method for shape representation and similarity measure called the grid-based method [1]. They have shown that the method is effective for the retrieval of segmented objects based on shape. In this paper, we describe a system which uses the grid-based method for retrieval of images with multiple objects. We perform experiments on the prototype system to compare the performance of the grid-based method with the Fourier descriptors method [2]. Preliminary results have been presented.

A hermeneutic analysis of the Denver international baggage handling system

This paper attempts to demonstrate the principles of hermeneutics in an effort to understand factors affecting Information Systems (IS) projects. As hermeneutics provides a systematic method of interpreting text from multiple information sources, thus, Information Systems being prima facie defined and documented as text documents, are eminently suited for this mode of investigation. In this paper, we illustrate hermeneutics by analysing a sample case study document describing a well known project of Denver International Airport (DIA) Automated Baggage Handling System, which was extensively reported in IS and management press and studied by Montealegre and his colleagues (Montealegre, Nelson, Knoop, & Applegate, 1999, p553-554). As a result of the hermeneutic approach to the analysis of this document, a new "flexibility" factor has been discovered to play an important, yet unreported, role in the DIA system demise. In the DIA case, the observed flexibility factor influenced the quality of the interaction between the actors, the prevailing environment and the information systems.

A defense system against DDoS attacks by large-scale IP traceback

In this paper, we present a new approach, called Flexible Deterministic Packet Marking (FDPM), to perform a large-scale IP traceback to defend against Distributed Denial of Service (DDoS) attacks. In a DDoS attack the victim host or network is usually attacked by a large number of spoofed IP packets coming from multiple sources. IP traceback is the ability to trace the IP packets to their sources without relying on the source address field of the IP header. FDPM provides many flexible features to trace the IP packets and can obtain better tracing capability than current IP traceback mechanisms, such as Probabilistic Packet Marking (PPM), and Deterministic Packet Marking (DPM). The flexibilities of FDPM are in two ways, one is that it can adjust the length of marking field according to the network protocols deployed; the other is that it can adjust the marking rate according to the load of participating routers. The implementation and evaluation demonstrates that the FDPM needs moderately only a small number of packets to complete the traceback process; and can successfully perform a large-scale IP traceback, for example, trace up to 110,000 sources in a single incident response. It has a built-in overload prevention mechanism, therefore this scheme can perform a good traceback process even it is heavily loaded.

Robust parallel filtering design of a swarm tracking system

Swarming networks of mobile autonomous agents require inter-agent position information in order perform various tasks. The primary control input for the majority of current control strategies is inter-agent distance information. In this paper we provide a robust parallel filter based tracking scheme that allows a mobile agent to track other multiple mobile agents. The distance, angle, and relative position is given in a direct target tracking output. This allows the mobile agent to decide which information is best suited for the particular objective

A new blind-equalization algorithm for an FIR SIMO system driven by MPSK signal

We consider the problem of blind equalization of a finite impulse response and single-input multiple-output system driven by an M-ary phase-shift-keying signal. The existing single-mode algorithms for this problem include the constant modulus algorithm (CMA) and the multimodulus algorithm (MMA). It has been shown that the MMA outperforms the CMA when the input signal has no more than four constellation points, i.e., Mles4. In this brief, we present a new adaptive equalization algorithm that jointly exploits the amplitude and phase information of the input signal. Theoretical analysis shows that the proposed algorithm has less mean square error, i.e., better equalization performance, at steady state than the CMA regardless of the value of M. The superior performance of our algorithm to the CMA and the MMA is validated by simulation examples

Identification and expression of natriuretic peptide receptor Type-A and-B mRNA in freshwater and seawater rainbow trout

Natriuretic peptide receptors mediate the physiological response of  natriuretic peptide hormones. One of the natriuretic peptide receptor types is the particulate guanylyl cyclase receptors, of which there are two identified: NPR-A and NPR-B. In fishes, these have been sequenced and characterized in eels, medaka, and dogfish shark (NPR-B only). The euryhaline rainbow trout provides an opportunity to further pursue examination of the system in teleosts. In this study, partial rainbow trout NPR-A-like and NPR-B-like mRNA sequences were identified via PCR and cloning. The sequence information was used in real-time PCR to examine mRNA expression in a variety of tissues of freshwater rainbow trout and rainbow trout acclimated to 35 parts per thousand seawater for a period of 10 days. In the excretory kidney and posterior intestine, real-time PCR analysis showed greater expression of NPR-B in freshwater fish than in those adapted to seawater; otherwise, there was no difference in the expression of the individual receptors in fresh water or seawater. In general, the expression of the NPR-A and NPR-B type receptors was quite low. These findings indicate that NPR-A and NPR-B mRNA expression is minimally altered under the experimental regime used in this study.

Suppression by ABA of salicylic acid and lignin accumulation and the expression of multiple genes, in Arabidopsis infected with Pseudomonas syringae pv. tomato

Abscisic acid (ABA) has been implicated in determining the outcome of interactions between many plants and their pathogens. We had previously shown that increased concentrations of ABA within leaves of Arabidopsis induced susceptibility towards an avirulent strain of Pseudomonas syringae pathovar (pv.) tomato. We now show that ABA induces susceptibility via suppression of the accumulation of components crucial for a resistance response. Lignin and salicylic acid concentrations in leaves were increased during a resistant interaction but reduced when plants were treated with ABA. The reduction in lignin and salicylic acid production was independent of the development of the hypersensitive response (HR), indicating that, in this host-pathogen system, HR is not required for resistance. Genome-wide gene expression analysis using microarrays showed that treatment with ABA suppressed the expression of many defence-related genes, including those important for phenylpropanoid biosynthesis and those encoding resistance-related proteins. Together, these results show that resistance induction in Arabidopsis to an avirulent strain of P. syringae pv. tomato is regulated by ABA.

Multiple-criteria decision analysis for integrated catchment management

Implementation of integrated catchment management (ICM) is hampered by the lack of a conceptual framework for explaining how landowners select farming systems for their properties. Benefit–cost analysis (a procedure that estimates the costs and benefits of alternative actions or policies) has limitations in this regard, which might be overcome by using multiple-criteria decision analysis (MCDA). MCDA evaluates and ranks alternatives based on a landowner's preferences (weights) for multiple-criteria and the values of those criteria. A MCDA approach to ICM is superior to benefit–cost analysis which focuses only on the monetary benefits and costs, because it: 1) recognizes that human activities within a catchment are motivated by multiple and often competing criteria and/or constraints; 2) does not require monetary valuation of criteria; 3) allows trade-offs between criteria to be measured and evaluated; 4) explicitly considers how the spatial configuration of farming systems in a catchment influences the values of criteria; 5) is comprehensive, knowledge-based, and stakeholder oriented which greatly increases the likelihood of resolving catchment problems; and 6) allows consideration of the fairness and sustainability of land and water resource management decisions. A MCDA based on an additive, multiple-criteria utility function containing five economic and environmental criteria was used to score and rank five farming systems. The rankings were based on the average criteria weights for a sample of 20 farmers in a US catchment. The most profitable farming system was the lowest-ranked farming system. Three possible reasons for this result are evaluated. First, the MCDA method might cause respondents to express socially acceptable attitudes towards environmental criteria even when they are not important from a personal viewpoint. Second, the MCDA method could inflate the ranks of less profitable farming systems for the simple reason that it allows the respondent to assign non-zero weights to non-economic criteria. Third, the MCDA might provide a better framework for evaluating a landowner's selection of farming systems than the profit maximization model.

Genomic analyses and cloning of novel chicken natriuretic peptide genes reveal new insights into natriuretic peptide evolution

The natriuretic peptide (NP) family consists of multiple subtypes in teleosts, including atrial, B-type, ventricular, and C-type NPs (ANP, BNP, VNP, CNP-1–4, respectively), but only ANP, BNP, CNP-3, and CNP-4 have been identified in tetrapods. As part of understanding the molecular evolution of NPs in the tetrapod lineage, we identified NP genes in the chicken genome. Previously, only BNP and CNP-3 have been identified in birds, but we characterized two new chicken NP genes by cDNA cloning, synteny and phylogenetic analyses. One gene is an orthologue of CNP-1, which has only ever been reported in teleostei and bichir. The second gene could not be assigned to a particular NP subtype because of high sequence divergence and was named renal NP (RNP) due to its predominant expression in the kidney. CNP-1 mRNA was only detected in brain, while CNP-3 mRNA was expressed in kidney, heart, and brain. In the developing embryo, BNP and RNP transcripts were most abundant 24 h post-fertilization, while CNP mRNA increased in a stage-dependant manner. Synthetic chicken RNP stimulated an increase in cGMP production above basal level in chicken kidney membrane preparations and caused a potent dose-dependant vasodilation of pre-constricted dorsal aortic rings. From conserved chromosomal synteny, we propose that the CNP-4 and ANP genes have been lost in chicken, and that RNP may have evolved from a VNP-like gene. Furthermore, we have demonstrated for the first time that CNP-1 is retained in the tetrapod lineage.

The cost of storing checkpoints to multiple volatile storage locations using at-least-k semantics

Using the volatile memory of other computers within a system to store checkpoints is an alternative to the traditional approach of using stable storage. The objective of this study is to develop a storage mechanism using at-least-k delivery semantics. This semantics allows data to be saved to a minimum number of computers simultaneously using group communications, without requiring that each group computer successfully acknowledge the receipt. The new storage mechanism is implemented in the GENESIS checkpointing facility v2.0. The results showed that at-least-k storage mechanisms provide low storage latency times; however, the incurred execution overheads on the applications executing within the system are higher than that when using remote stable storage to store checkpoints.