Effects of glutamine supplementation on kidney of diabetic rat

Glutamine is the most important donor of NH(3) in kidney playing an important role in acid-base buffering system. Besides this effect, glutamine presents many other relevant functions in the whole body, such as a precursor of arginine in adult and neonates. In addition to these effects, some studies have shown that glutamine can potentiate renal disease. In the present study, the effect of short-term treatment (15 days) with glutamine on control and diabetic rats was investigated. Using biochemical, histological and molecular biology analysis from control and diabetic rats we verified that glutamine supplementation increase in pro-inflammatory interleukins (IL)-1 beta and IL-6 content in renal cortex and induce alteration in glomerular characteristics. This study showed that short-term treatment with glutamine in association with increased glucose levels could cause important alterations in glomerular morphology that may result in fast progression of kidney failure.

Delayed local inflammatory response induced by Thalassophryne nattereri venom is related to extracellular matrix degradation

Symptoms evoked by Thalassophryne nattereri fish envenomation include local oedema, severe pain and intense necrosis with strikingly inefficient healing, continuing for several weeks or months. Investigations carried out in our laboratory showed that, in the venom-induced acute inflammation, thrombosis in venules and constrictions in arterioles were highly visible, in contrast to a notable lack of inflammatory cell. Nevertheless, the reason that the venom toxins favour delayed local inflammatory response is poorly defined. In this study, we analysed the movement of leucocytes after T. nattereri venom injection in the intraplantar region of Swiss mice, the production of pro-inflammatory mediators and the venom potential to elicit matrix metalloproteinase production and extracellular matrix degradation. Total absence of mononuclear and neutrophil influx was observed until 14 days, but the venom stimulates pro-inflammatory mediator secretion. Matrix metalloproteinases (MMP)-2 and MMP-9 were detected in greater quantities, accompanied by tissue degradation of collagenous fibre. An influx of mononuclear cells was noted very late and at this time the levels of IL-6, IL-1 beta and MMP-2 remained high. Additionally, the action of venom on the cytoskeletal organization was assessed in vitro. Swift F-actin disruption and subsequent loss of focal adhesion was noted. Collectively these findings show that the altered specific interaction cell-matrix during the inflammatory process creates an inadequate environment for infiltration of inflammatory cells.

Modulation of inflammatory response by selective inhibition of cyclooxygenase-1 and cyclooxygenase-2 in acute kidney injury

This work explored the role of inhibition of cyclooxygenases (COXs) in modulating the inflammatory response triggered by acute kidney injury. C57Bl/6 mice were used. Animals were treated or not with indomethacin (IMT) prior to injury (days -1 and 0). Animals were subjected to 45 min of renal pedicle occlusion and sacrificed at 24 h after reperfusion. Serum creatinine and blood urea nitrogen, reactive oxygen species (ROS), kidney myeloperoxidase (MPO) activity, and prostaglandin E2 (PGE(2)) levels were analyzed. Tumor necrosis factor (TNF)-alpha, t-bet, interleukin (IL)-10, IL-1 beta, heme oxygenase (HO)-1, and prostaglandin E synthase (PGES) messenger RNA (mRNA) were studied. Cytokines were quantified in serum. IMT-treated animals presented better renal function with less acute tubular necrosis and reduced ROS and MPO production. Moreover, the treatment was associated with lower expression of TNF-alpha, PGE(2), PGES, and t-bet and upregulation of HO-1 and IL-10. This profile was mirrored in serum, where inhibition of COXs significantly decreased interferon (IFN)-gamma, TNF-alpha, and IL-12 p70 and upregulated IL-10. COXs seem to play an important role in renal ischemia and reperfusion injury, involving the secretion of pro-inflammatory cytokines, activation of neutrophils, and ROS production. Inhibition of COX pathway is intrinsically involved with cytoprotection.

Enhanced Neural Progenitor/Stem Cells Self-Renewal via the Interaction of Stress-Inducible Protein 1 with the Prion Protein

Prion protein (PrPC), when associated with the secreted form of the stress-inducible protein 1 (STI1), plays an important role in neural survival, neuritogenesis, and memory formation. However, the role of the PrP(C)-STI1 complex in the physiology of neural progenitor/stem cells is unknown. In this article, we observed that neurospheres cultured from fetal forebrain of wild-type (Prnp(+/+)) and PrP(C)-null (Prnp(0/0)) mice were maintained for several passages without the loss of self-renewal or multipotentiality, as assessed by their continued capacity to generate neurons, astrocytes, and oligodendrocytes. The homogeneous expression and colocalization of STI1 and PrP(C) suggest that they may associate and function as a complex in neurosphere-derived stem cells. The formation of neurospheres from Prnp(0/0) mice was reduced significantly when compared with their wild-type counterparts. In addition, blockade of secreted STI1, and its cell surface ligand, PrP(C), with specific antibodies, impaired Prnp(+/+) neurosphere formation without further impairing the formation of Prnp(0/0) neurospheres. Alternatively, neurosphere formation was enhanced by recombinant STI1 application in cells expressing PrP(C) but not in cells from Prnp(0/0) mice. The STI1-PrP(C) interaction was able to stimulate cell proliferation in the neurosphere-forming assay, while no effect on cell survival or the expression of neural markers was observed. These data suggest that the STI1-PrP(C) complex may play a critical role in neural progenitor/stem cells self-renewal via the modulation of cell proliferation, leading to the control of the stemness capacity of these cells during nervous system development. STEM CELLS 2011;29:1126-1136

Maslov index in semi-Riemannian submersions

We study focal points and Maslov index of a horizontal geodesic gamma : I -> M in the total space of a semi-Riemannian submersion pi : M -> B by determining an explicit relation with the corresponding objects along the projected geodesic pi omicron gamma : I -> B in the base space. We use this result to calculate the focal Maslov index of a (spacelike) geodesic in a stationary spacetime which is orthogonal to a timelike Killing vector field.

Evidence that OGG1 glycosylase protects neurons against oxidative DNA damage and cell death under ischemic conditions

7,8-Dihydro-8-oxoguanine DNA glycosylase (OGG1) is a major DNA glycosylase involved in base-excision repair (BER) of oxidative DNA damage to nuclear and mitochondrial DNA (mtDNA). We used OGG1-deficient (OGG1(-/-)) mice to examine the possible roles of OGG1 in the vulnerability of neurons to ischemic and oxidative stress. After exposure of cultured neurons to oxidative and metabolic stress levels of OGG1 in the nucleus were elevated and mitochondria exhibited fragmentation and increased levels of the mitochondrial fission protein dynamin-related protein 1 (Drp1) and reduced membrane potential. Cortical neurons isolated from OGG1(-/-) mice were more vulnerable to oxidative insults than were OGG1(+/+) neurons, and OGG1(-/-) mice developed larger cortical infarcts and behavioral deficits after permanent middle cerebral artery occlusion compared with OGG1(+/+) mice. Accumulations of oxidative DNA base lesions (8-oxoG, FapyAde, and FapyGua) were elevated in response to ischemia in both the ipsilateral and contralateral hemispheres, and to a greater extent in the contralateral cortex of OGG1(-/-) mice compared with OGG1(+/+) mice. Ischemia-induced elevation of 8-oxoG incision activity involved increased levels of a nuclear isoform OGG1, suggesting an adaptive response to oxidative nuclear DNA damage. Thus, OGG1 has a pivotal role in repairing oxidative damage to nuclear DNA under ischemic conditions, thereby reducing brain damage and improving functional outcome. Journal of Cerebral Blood Flow & Metabolism (2011) 31, 680-692; doi:10.1038/jcbfm.2010.147; published online 25 August 2010

Vägen till jämställdhet - Kvinnor i styrelserum eller män i förskolan? : En kritisk analys av den svenska jämställdhetsdiskursen

Equality between the sexes has been discussed in Sweden for more than 50 years. In the 60´s and during the 70´s the discourse that dominated the debate was based on the assumption that both men and women needed to liberate themselves from their traditional gender roles. In this approach information and education was perceived as the key to equality. During the 80´s however, power and subordination became the main focal point of concern within this debate, and focus upon changing the patriarchal power structures dominating society were perceived as the principal key for establishing equality between the sexes. Today, the latter discourse still dominates both the scientific and the political perspective upon equality. By examining the debate on equality from two different fields of occupation this paper tries to analyse the fact that the Swedish equality discourse looks very different on male - versus female domination. Although the proportion of male teachers in Swedish preschools is about 3 % and the amount of women serving as public company directors is about 20 %, the current discourse views male dominance within the business sector as problematic, whilst, the dominance of women in the preschool childcare sector as less problematic. In respect of the theory supported by Bourdieu (1999), Hirdman (1990 & 2003) and Foucault (1998 & 2002) this paper advocates that the discourse is biased and simplified and that a perspective that only focuses on areas of male domination sets the wrong priorities. With regards to equality ideals prevalent throughout Norway this paper concludes that the Swedish equality discourse needs a broader and more open approach to assure that Swedish institutions promote equality between men and women in the best possible way.

Universal Experimental Measurement System «Sun-Walker» : Automotive measurement system for the evaluation of the solar irradiation distribution for the tests of the solar concentrated systems.

This Thesis project is a part of the research conducted in Solar industry. ABSOLICON Solar Concentrator AB has invented and started production of the prospective solar concentrated system Absolicon X10. The aims of this Thesis project are designing, assembling, calibrating and putting in operation the automatic measurement system intended to evaluate distribution of density of solar radiation in the focal line of the concentrated parabolic reflectors and to measure radiation from the artificial source of light being a calibration-testing tool.On the basis of the requirements of the company’s administration and needs of designing the concentrated reflectors the operation conditions for the Sun-Walker were formulated. As the first step, the complex design of the whole system was made and division on the parts was specified. After the preliminary conducted simulation of the functions and operation conditions of the all parts were formulated.As the next steps, the detailed design of all the parts was made. Most components were ordered from respective companies. Some of the mechanical components were made in the workshop of the company. All parts of the Sun-Walker were assembled and tested. The software part, which controls the Sun-Walker work and conducts measurements of solar irradiation, was created on the LabVIEW basis. To tune and test the software part, the special simulator was designed and assembled.When all parts were assembled in the complete system, the Sun-Walker was tested, calibrated and tuned.

Barn och föräldrars val av bilderbok med fokus på illustrationer : En intervju- och observationsundersökning

Syftet med denna studie var att dels jämföra barn, 3–6 år, och föräldrars preferenser av barnboksillustrationer, dels att undersöka hur föräldrar resonerar kring val av bilderbok och illustrationens roll i samband med detta. Genom enskilda semistrukturerade intervjuer och tillhörande observationer med 22 barn (3–6 år) och 10 föräldrar undersöktes vilken av fem typer av bokillustrationer som föredrogs. Föräldrarna fick därefter även välja vilken av fem bilderböcker, från vilka illustrationerna var hämtade, som de skulle vilja läsa för sina barn och motivera sitt val. Resultatet från undersökningen tyder på att illustrationerna är en aspekt för både barn och föräldrar vid val av bilderbok samt att det finns faktorer som föredras i illustrationerna. Barnen i undersökningen verkade föredra naivistiska illustrationer med stort antal fokuspunkter. Föräldrar föredrog realistiska illustrationer med inslag de kan koppla till verkliga livet. Föräldrarna ansåg att illustrationer, handling, moral och lärdom var viktiga aspekter vid valet av bilderbok till deras barn. Flertalet föräldrar ville ha möjlighet att berätta egna historier kring illustrationerna i bilderboken.

Humorforskning i svenska organisationer och arbetslivssammanhang : en litteraturöversikt

Research on humor in Swedish organizational and working life contexts: a literature review The objective of this study was to provide a more coherent picture of research on humor in Swedish organizational and working life contexts, where even non-strictly organizational studies were included due to their high relevance in the research area. A systematic review of the literature published during the last 15 years was used to summarize an obviously undeveloped research area in Sweden. Published peer-reviewed articles that primarily examine humor were analyzed. Seventeen representative articles have been found and systematically reviewed and compared focusing on four questions: purposes and contexts of the studies, theoretical approaches, methods and main results. The analysis identifies four focal points that describe current state of humor research in Sweden: 1) positive function of humor appears to be a predominant research issue while humor’s subversive function, power relations and negative functions are less represented, 2) limited comparative studies of different contexts 3) biased theoretical approaches and limited studies of theory developing approach 4) overrepresented qualitative research approach. The above issues were discussed in relation to relevant international humor research.

Om lärarskapets moraliska dimension : ett perspektiv och en studie av lärarstuderandes nätbaserade seminariesamtal

In this dissertation, an educational perspective called the moral dimension of teaching is developed. The work includes a theoretically informed discussion from a pragmatist point of view in which the concept of pedagogical rhythm is introduced. The concept captures the need for teachers to regularly shift their intentions and occasionally act in contradictory ways as a consequence of the moral which emerges from interaction in pedagogical situations. Using this perspective, criteria are developed for the characteristics of discussions of the work of teachers, which are desirable in order for students in pre-service teacher education to have opportunities to develop their teachership. Secondly, the educational perspective as it is conceptualised serves as a theoretical framework for a study of discussions taking place in net-based seminars among students in teacher education. The study consists of 14 recorded seminars in which discussions of the work of teachers are analysed in terms of content and direction for reflection. The result of the analysis is a construction of four different focal points for processes of making judgements: existential, performative, critical and professional. Mainly the performative, and to some extent the critical, focal points appear to be supported by the net-based environment, although potential for the professional focal point is found when available tools in net-based settings are used in deliberate ways. Finally, based on these four focal points, possible future predispositions among student teachers are deliberated. Student teachers’ future opportunities to develop a moral and epistemological authority are discussed, as well as teachers’ general opportunities to exercise professional responsibility. The conclusion emphasises that a perspective such as the one developed in the dissertation is important, as it creates an understanding for the need to educate student teachers to exercise a form of responsibility that goes beyond being accountable to society.

Where do we go from here? Possibilities in a convergent future

Soon after the French Revolution started in 1789, the great English poet William Wordsworth penned these memorable words: "Bliss was it at that time to be alive, but to be young was very heaven." He captured the excitement of a watershed period of human endeavors for his audience} but noted that the future belonged to the young. That is the message of this final chapter: that the future is bright for college graduates who are prepared to work hard. Fortune favors the prepared mind. The future is bright for journalists and journalism, and that future offers multiple possibilities for people who are prepared. It helps, though, to pause briefly to look at the past and to see what we can learn from it. As former British Prime Minister Winston Churchill once noted, the person who ignores history is condemned to repeat its mistakes.

What is convergence and how will it affect my life?

Convergence is a revolutionary and evolutionary form of journalism that is emerging in many parts of the world. As a student graduating from a journalism program in the early 21 st century, you need to know about convergence because it is likely to influence the way your career evolves. Over the course of the next decade, you probably will work in several media platforms, perhaps with different companies, but also possibly with the same company but doing varied forms of journalism. All journalists need to know how to tell stories in all media, and how to write appropriately for those media, as preparation for this new era.

Multimedia journalism: putting it all together

A combination of sound, image, text, and interactivity-hereafter referred to as multimedia journalism-gives media practitioners a new way to tell stories, using the strengths of each medium to produce a more compelling package. From the outset, a multimedia journalist must appreciate the potential and power of each medium and capitalize on those strengths. In doing so the multimedia reporter produces journalism that is well beyond what a single medium can do. The multimedia reporter needs to know how to use a variety of digital tools, but the essential requirement is a multimedia mind-set. Loosely articulated, this mind-set requires the ability to conceive of stories that go beyond a single medium when it is appropriate to use more than one medium. Sometimes one medium is sufficient to provide what audiences need. Implicit in this statement is an understanding of audiences (note the plural form) and an acceptance that the fundamental role of journalism remains constant, which is to inform, educate, and entertain.

The minimum moral content of law : a critique of Hart's descriptive theory of positive and natural law

Review of H.L.A. Hart's account of the minimum moral content of law - assesses its consistency with the methodology provided in his description of the focal meaning or central case of law - particular focus is Hart's consideration of the ultimate end of man - how difficulties faced by Hart's account of the minimum moral content of law can be overcome.