Desempenho de sementes de couve-flor em função de níveis de vigor e resposta à aplicação de nitrogênio

Pós-graduação em Agronomia - FEIS

Testes de vigor em sementes de quiabeiro

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Germinação e vigor de sementes de soja em função da disponibilidade hídrica do solo e presença de Phomopsis sojae

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação do potencial fisiológico de sementes de mutamba (Guazuma ulmifolia Lam.) por meio de diferentes testes de vigor

Pós-graduação em Agronomia - FCAV

Teste de alagamento, deterioração controlada e envelhecimento acelerado para avaliação do vigor de sementes de feijão

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Assessment of the physiological potential of spinach seeds (Tetragonia tetragonoides (Pall.) Kuntze)

The efficiency of vigor tests in assessing the physiological potential of seeds depends on their standardization for different species. In this context, the research aimed to study specific methodologies to evaluate the vigor of spinach seeds (Tetragonia tetragonoides). For this purpose, five lots of spinach seed cv. New Zealand were submitted to germination, first count of germination, seedling emergence in the field, accelerated aging (with and without use of saturated solution of NaCl at 41and 45 ºC for 24,48 and 72 hours), controlled deterioration (18,21 and 24% seed water content adjustments for 24 hours at 45 ºC) and electrical conductivity tests with variations inwater volume (25, 50 and 75 mL), seed quantity (25 and 50) and soaking period (1, 2, 4, 8,12, 16 and 24 hours) at 25 ºC. The first count test allowed obtaining preliminary information about seed vigor, and other tests in general showed similarity with seedling emergence in the field. Thus, it was concluded that traditional accelerated aging test 24 h /41 ºC and accelerated aging test with saturated salt solution for 24 h at 41 ºC and 45 ºC, and controlled deterioration test with 21% seed water content / 24 h / 45 ºC were all efficient for evaluating the physiological potential of spinach seeds.

Testes para a seleção de populações de cenoura visando ao vigor e à longevidade das sementes

Populations of carrot seeds with superior physiological quality and storage potential are of interest to seed companies and growers. Thus, we verified the efficiency of some tests for the selection of carrot populations with greater vigor and longevity of seeds. Seeds of 50 carrots progenies of different half-brothers from Brasilia cultivar were evaluated for the mass of one hundred seeds, the first count, germination, dormancy, accelerated aging with water and saturated NaCl solution. The seeds were stored at moisture contents of 6.1±0.3% in hermetic packaging at temperatures of 15 and 25°C for 12 months and germination was evaluated quarterly. The experimental data were evaluated for variance and phenotypic, genotypic and environmental heritability, coefficient of variation and genetic gain from selection. Selection based on the mass should not be used because it would increase the occurrence of dormancy in seeds of the next generation. The test of the first count, germination and accelerated aging in water or saline solution saturated may be used to select populations of carrot seeds of higher vigor and longevity. The estimated gain genetic selection for germination after 12 months storage at 25°C was 14%.

Efeito do estresse hídrico e salino na germinação e vigor de sementes de maxixe

The objective of this work was to evaluate the germination and vigor of two lots of gherkin seeds in different osmotic potentials using polyethylene glycol (PEG 6000) and sodium chloride (NaCl). Seeds of the 'North' cultivar were sown in germitest paper moistened in solutions of polyethylene glycol and sodium chloride in osmotic potentials of 0, -0.3, -0.6 and -0.9MPa. The experimental design was completely randomized in a factorial 2x4 design (solutions x osmotic potential), with four replications. Seed vigor was evaluated by the germination test, first germination count, length and dry weight of root and hypocotyl. It is concluded that the reduction in osmotic potential from -0.3MPa induced by PEG 6000 and NaCl reduces the germination and vigor of gherkin's seeds. The osmotic stress induced by PEG 6000 produced more severe effects than saline in physiological quality of gherkin's seeds.

Umedecimento do substrato e temperatura na germinação e vigor de sementes de melão

The study was conducted to evaluate the efficacy of herbicides in desiccation Raphanus raphanistrum (wild radish) in different application times and their effects on weed seed germination. The experimental had a randomized block design with three replications. The herbicides used were: glyphosate (360; 720; and 1080 g ha(-1)), 2,4-D (335; 670; and 1005 g ha(-1)), glyphosate + 2,4-D (360 + 335; 720 + 670; and 1080 + 1005 g ha(-1)). Spraying was conducted at three different times: 1st season (full bloom) - at 62 days after emergence (DAE), 2nd season (beginning of pod formation) - at 92 DAE and 3rd season (end of pod formation) - at 108 DAE. At 14, 21, 28, 35, and 42 days after application (DAA), the desiccation was evaluated visually, and 42 DAA dry mass of shoots and seeds of the 2nd and 3rd times were collected for the test of germination. All chemical treatments tested were effective in controlling the plants of wild radish in the 1st and 2nd times, but in the 3rd time doses of 2,4-D applied singly were not effective and only the highest dose of glyphosate applied singly or in mixture provided a total plant control. In terms of percentage, the reduction in dry matter of plants was higher with the application of larger doses of chemical treatments. The herbicides affected the germination of seeds of wild radish, and the herbicide 2,4-D provided greater reduction in germination in the 2nd and 3rd seasons.

STORAGE OF Handroanthus umbellatus SEEDS

Seed storage under controlled environmental conditions represents one of the most important lines of research to be applied on short-lived forest species as Handroanthus. The present research aimed to identify the most suitable seed storage conditions and longevity behavior of Handroanthus umbellatus seeds subject to the following storage treatments: packaging permeable paper bags under a no-controlled laboratory temperature and humidity (control) and multiwall semipermeable bag at temperatures of -18 degrees C ,1 degrees C and 25 degrees C. Seeds were dried to 6.3% of water content. Stored seeds were evaluated every three months until 24 months for water content, germination percentage and vigor utilizing first counting test. Seeds of T. umbellata are orthodox, with low longevity under natural conditions, once they remain viable for less than 5 months. The best conditions of seed preservation of these species were obtained by storage at -18 degrees C in multiwall bags. Under these conditions physiological seed quality remains unchanged for a 24-month period.

GERMINATION OF EUCALYPTUS SEEDS UNDER WATER AND SALT STRESS

The knowledge of the physiology of Eucalyptus spp. germination may contribute significantly to the development of management and choice of suitability of the deployment areas. The aim was to evaluate the effects of water and salt stress on seed germination of Eucalyptus camaldulensis, E. citriodora, E. grandis, E. robusta and E. urophylla. The seeding was done with four replicates of 0.05 g of seeds in paper moistened with solutions at potentials of 0.0, -0.2, -0.4, and -0.8 MPa, induced with polyethylene glycol (PEG 6000) and NaCl. The germination test was in 25 degrees C in the presence of light. Were evaluated the first test score seven days after sowing, and weekly germination (normal seedlings) until 28 days. Were also calculated the germination speed index. Water stress causes a greater reduction in the rate of germination and accumulated germination of E. camaldulensis and E. citriodora seeds than salt stress, and the seeds of E. robusta are more adapted to germinate under salt stress moderate, between -0.2 and -0.4 MPa. Regardless of the substance used to induce stress, the threshold for germination was -0.8 MPa. The E. camaldulensis is the most sensitive specie to water stress and E. urophylla most sensitive to salt stress.

Testes de estresse na avaliação do vigor de sementes de milho

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Accelerated aging and electrical conductivity tests in crambe seeds

The aim of this study was to adapt the methodology of the accelerated aging and electrical conductivity tests for determination of physiological potential in crambe seeds. Six seed lots of crambe (cv. FMS Brilhante) were subjected to determination of moisture content, germination test, first count germination, emergence, and emergence speed index. For the accelerated aging test, the traditional methodology was used with water, and with a saturated potassium chloride and sodium chloride solution in three periods of exposure (24, 48, and 72 hours) at 41 degrees C; the electrical conductivity test was performed with four pre-soaking treatments (0, 2, 4, and 8 hours) and four soaking periods (4, 8, 16, and 24 hours) at 25 degrees C. The accelerated aging test with water for 72 hours and the electrical conductivity test with 2 hours of pre-soaking and assessment after 16 hours were effective for classification of the crambe seed lots in regard to physiological quality.

Xenia Effects on Quality of Maize Female Inbred in Production of Hybrid Seeds

In Maize (Zea maize L.), cost of hybrid seed production is directly related to the yield and quality of seed obtained per hectare of female parent. It is also important to consider the effects that a male parent can exert on the development of hybrid seed in the female parent. This effect is known as xenia. The objectives of this study were to evaluate xenia effects on 1) yield as 80K units, 2) germination of the hybrid seed and 3) susceptibility of the hybrid seed to mechanical damage. One female inbred and four male inbred lines were selected from a parent list of hybrids. The experiment was designed to allow individual cross pollination between each male inbred and the female inbred line. For use as a control, the female inbred was allowed to self pollinate. Experiments were conducted in Illinois and Iowa during 2008 and 2009 and in Nebraska during 2009. A significant inbred effect was detected on yield as 80k (α=0.001). The selfed female and pollination with male inbred B resulted in lower yields of hybrid seed. For germination, a significant inbred effect was detected (α=0.001), but was due to lower germination percentage of seed produced on the selfed female. All hybrid combinations resulted in higher germination percentages with no significant differences among hybrids. The inbred x mechanical damage interaction was significant (P=0.04) for effects on cold saturated soil germination tests. Use of inbred B resulted in a two-percentage-point reduction in cold germination when treated with the impact simulator. In a maize seed company, the production research group provides yield estimates for production of new hybrid combinations. Results from this study indicate that using only the female inbred yield may provide inaccurate estimates. Therefore to improve yield estimation, experiments should be designed to include male inbreds. Male inbreds can also impart a negative effect to the hybrid seed on tolerance to mechanical damage, thus lowering quality and increasing seed discard. When testing for hybrid seed germination, there is no need to consider distinct hybrid combinations. Female inbreds can be grown in open-pollinated fields to avoid loss of vigor observed with selfing. Advisor: George Graef

Automated system of seedling image analysis (SVIS) and electrical conductivity to assess sun hemp seed vigor

The development of new procedures for quickly obtaining accurate information on the physiological potential of seed lots is essential for developing quality control programs for the seed industry. In this study, the effectiveness of an automated system of seedling image analysis (Seed Vigor Imaging System - SVIS) in determining the physiological potential of sun hemp seeds and its relationship with electrical conductivity tests, were evaluated. SVIS evaluations were performed three and four days after sowing and data on the vigor index and the length and uniformity of seedling growth were collected. The electrical conductivity test was made on 50 seed replicates placed in containers with 75 mL of deionised water at 25 ºC and readings were taken after 1, 2, 4, 8 and 16 hours of imbibition. Electrical conductivity measurements at 4 or 8 hours and the use of the SVIS on 3-day old seedlings can effectively detect differences in vigor between different sun hemp seed lots.