77 resultados para Enolase


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Fasciola hepatica, commonly known as liver fluke, is a trematode which causes Fasciolosis in ruminants and humans. The outer tegumental coat of F. hepatica (FhTeg) is a complex metabolically active biological matrix that is continually exposed to the host immune system and therefore makes a good vaccine target. F. hepatica tegumental coat is highly glycosylated and helminth-derived immunogenic oligosaccharide motifs and glycoproteins are currently being investigated as novel vaccine candidates. This report presents the first systematic characterisation of FhTeg glycosylation using lectin microarrays to characterise carbohydrates motifs present, and lectin histochemistry to localize these on the F. hepatica tegument. We discovered that FhTeg glycoproteins are predominantly oligomannose oligosaccharides that are expressed on the spines, suckers and tegumental coat of F. hepatica and lectin blot analysis confirmed the abundance of N- glycosylated proteins. While some oligosaccharides are widely distributed on the fluke surface other subsets are restricted to distinct anatomical regions. We selectively enriched for FhTeg mannosylated glycoprotein subsets using lectin affinity chromatography and identified 369 proteins by mass spectrometric analysis. Among these proteins are a number of potential vaccine candidates with known immune modulatory properties including proteases, protease inhibitors, paramyosin, Venom Allergen-like II, Enolase and two proteins, nardilysin and TRIL, that have not been previously associated with F. hepatica Furthermore, we provide a comprehensive insight regarding the putative glycosylation of FhTeg components which could highlight the importance of further studies examining glycoconjugates in host-parasite interactions in the context of F. hepatica infection and the development of an effective vaccine.

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Cette étude a pour objectif de déterminer la valeur pronostique de la protéine S-100ß et de l’énolase neurone-spécifique (NSE) chez les patients ayant subi un traumatisme craniocérébral (TCC) modéré ou grave. Deux revues systématiques et méta-analyses ont été effectuées afin de recenser les études présentant un dosage de ces biomarqueurs en lien avec la mortalité ou le pronostic fonctionnel évalué à l’aide du score du Glasgow outcome scale (GOS). Des 9228 résultats de la recherche, 41 et 26 études ont été incluses, respectivement, pour la protéine S-100ß et la NSE. Il existe une association entre le dosage sérique de la protéine S-100ß et de la NSE avec une issue clinique défavorable, c’est-à-dire la mortalité ou un score du GOS ≤ 3. Une concentration sérique entre 1,38 et 10,50 µg/L pour la protéine S-100ß est 100 % spécifique pour prédire le décès. La présence de lésions extracérébrales n’influençait pas cette association.