Changing Lived Worlds of Contemporary Amazonian Native Young People : Manchineri Youths in the Reserve and the City, Brazil-Acre

This thesis discusses the contemporary construction of the lived worlds of indigenous Amazonian youths. Today’s native peoples are considerably affected by the processes of globalization and urbanization, which have led to new ways of relating to their cultural traditions. This work presents a case study of Manchineri youngsters aged between 14 and 24 years old living in Acre state in Brazilian Amazonia. The Arawak-speaking Manchineri number some 1,000 people; their legally demarcated reserve is situated next to the River Yaco. The research is based on ethnographic material collected in the Mamoadate reserve and in the state capital, Rio Branco. By comparing the youth in different physical and social environments (the reserve and the city), my attempt has been to search for the most typical elements maintained, altered and created in the current lived worlds of Manchineri youths. Fieldwork methods included interviews, participant observation, photographs, video recordings, and drawings. The material was analyzed within the multidisciplinary framework of the social and cultural construction of knowledge. The study applies the concepts of social field, symbolic capital, and habitus as they have been used by Pierre Bourdieu; perspective as developed recently in Amazonian ethnology; the sacred as a cultural category as understood in the study of religion; and individual and person as concepts central to anthropology and sociology. Additionally, the study can be contextualized within youth studies, Latin American studies, and urban studies. The results of the study show that the everyday lives of young Amazonian native people are formed by a complex mixture of ‘modernity’ and ‘tradition’, fragmentation, and transitions between different conceptual frameworks. Part II discusses the ethnographic material in depth and shows that indigenous adolescents act from a variety of social perspectives: the native youth’s own ethnic group, divided into sub-groups, especially into urban residents and those living in the reserve; ancestors, super-human agents and spirits; other indigenous groups and non-natives. Consequently, besides the traditional initiation ritual, we find various contemporary rites of passage to adulthood: state-education, learning traditional practices, shamanism, matrimony, and transitions between the reserve and urban areas. According to these results, new social roles, political organization, responsibilities, and in general the desire to be respected, require both ‘modern’ and ‘traditional’ abilities. In Part III, the study shows that the current power relations constituted by new social contacts, ethnic recognition, and cooperation with different institutions have resulted in the formation of new social fields: youth cultures, the ethnic group, shamanic practices, the ethnopolitical movement, and indigenous students. The capacity of young Amazonian Indians to act in contemporary social fields produces them as full persons. The study also argues that the elements of the lived worlds can be divided into these social fields. When focusing on these fields, it became evident that these comprise the strategies adopted by young Indians to break through social and cultural barriers.

La Aplicación de estadísticas y de los SIG en el estudio de las construcciones monumentales prehistóricas de la Amazonía brasileña

Las estructuras monumentales prehistóricas construidas mediante zanjas continuas excavadas en el suelo de la Amazonia brasileña, son yacimientos que comprenden varias formas geométricas de diversos tamaños. Actualmente se conocen 291 yacimientos arqueológicos, la mayor parte de los cuales han sido mapeados por medio de imágenes de satélite. Estas construcciones prehistóricas fueron localizadas mediante la combinación de una serie de estrategias de prospección que incluyen el uso de imágenes satélites, sobrevuelos y otras tecnologías que posibilitaron su identificación. En un análisis de caracterización cuantitativa y morfológica y a través da estadísticas, obtuvimos como principales resultados que existe una gran dispersión de los datos correspondientes a su tamaño, siendo menor la variación para la profundidad, el valor medio de las áreas de los recintos con zanjas perimetrales es 17.490,6 m2 , a pesar de que el 40,3% mide menos de una hectárea (10.000 m ), la altitud media a la que se encuentran es de 194,4 metros y ésta es la variable que mejor correlación tiene con la posición geográfica. Con la ayuda de gvSIG, Sextante, GRASS y R, hemos tratado de caracterizar la ubicación de los yacimientos atendiendo a diferentes variables entre las que destacan la altitud relativa, la orientación, la distancia al curso de agua más próximo, la pendiente y la posición relativa en el territorio. La intención es intentar predecir en qué áreas, hoy en día cubiertas por la masa forestal, se pueden encontrar estructuras semejantes a las localizadas en las áreas abiertas. Este carácter predictivo de nuestras observaciones sería de vital importancia para poder definir futuras prospecciones en las áreas boscosas de la Amazonia.Para el futuro, no descartamos el aprovechamiento de datos LIDAR para intentar comprobar si las áreas designadas como potencialmente poseedoras de geoglifos efectivamente los poseen

Mosquito (Diptera: Culicidae) Diversity of a Forest-Fragment Mosaic in the Amazon Rain Forest

To study the impact of Amazonian forest fragmentation on the mosquito fauna, an inventory of Culicidae was conducted in the upland forest research areas of the Biological Dynamics of Forest Fragments Project located 60 km north of Manaus, Amazonas, Brazil. The culicid community was sampled monthly between February 2002 and May 2003. CDC light traps, flight interception traps, manual aspiration, and net sweeping were used to capture adult specimens along the edges and within forest fragments of different sizes (1, 10, and 100 ha), in second-growth areas surrounding the fragments and around camps. We collected 5,204 specimens, distributed in 18 genera and 160 species level taxa. A list of mosquito taxa is presented with 145 species found in the survey, including seven new records for Brazil, 16 new records for the state of Amazonas, along with the 15 morphotypes that probably represent undescribed species. No exotic species [Aedes aegypti (L.) and Aedes albopictus (Skuse)] were found within the sampled areas. Several species collected are potential vectors of Plasmodium causing human malaria and of various arboviruses. The epidemiological and ecological implications of mosquito species found are discussed, and the results are compared with other mosquito inventories from the Amazon region.

Rural property size drives patterns of upland and riparian forest retention in a tropical deforestation frontier

Deforestation in Brazilian Amazonia accounts for a disproportionate global scale fraction of both carbon emissions from biomass burning and biodiversity erosion through habitat loss. Here we use field- and remote-sensing data to examine the effects of private landholding size on the amount and type of forest cover retained within economically active rural properties in an aging southern Amazonian deforestation frontier. Data on both upland and riparian forest cover from a survey of 300 rural properties indicated that 49.4% (SD = 29.0%) of the total forest cover was maintained as of 2007. and that property size is a key regional-scale determinant of patterns of deforestation and land-use change. Small properties (<= 150 ha) retained a lower proportion of forest (20.7%, SD = 17.6) than did large properties (>150 ha; 55.6%, SD = 27.2). Generalized linear models showed that property size had a positive effect on remaining areas of both upland and total forest cover. Using a Landsat time-series, the age of first clear-cutting that could be mapped within the boundaries of each property had a negative effect on the proportion of upland, riparian, and total forest cover retained. Based on these data, we show contrasts in land-use strategies between smallholders and largeholders, as well as differences in compliance with legal requirements in relation to minimum forest cover set-asides within private landholdings. This suggests that property size structure must be explicitly considered in landscape-scale conservation planning initiatives guiding agro-pastoral frontier expansion into remaining areas of tropical forest. (C) 2010 Elsevier Ltd. All rights reserved.

The spider genus Chrysometa (Araneae, Tetragnathidae) from the Pico da Neblina and Serra do Tapirapeco mountains (Amazonas, Brazil): new species, new records, diversity and distribution along two altitudinal gradients

Eight new species of the spider genus Chrysometa Simon, 1894 (Araneae, Tetragnathidae) are described and illustrated. Chrysometa nubigena n. sp., C. waikoxi n. sp., C. petrasierwaldae n. sp., C. santosi n. sp., C. yanomami n. sp., C. candianii n. sp., C. lomanhungae n. sp., and C. saci n. sp. Those species were collected in a study on the diversity of spider communities along altitudinal gradients in Brazilian Amazonia. C. saci was captured at the Serra do Tapirapeco (Barcelos), while all the other species are from the Pico da Neblina (Sao Gabriel da Cachoeira), the highest mountain in Brazil. We provide new records for C. boraceia, C. flava, C. guttata, C. minuta and C. opulenta, and we describe the male of C. minuta for the first time. We also present the first results on the diversity and altitudinal distribution of the species of Chrysometa at the Pico da Neblina and Serra do Tapirapeco. We sampled the first locality at six different elevations, and obtained 336 specimens distributed in 12 species. Richness and abundance, as well as relative importance peaked at the highest sites sampled (2,000 and 2,400 m). The three most abundant species showed a segregated distribution, being dominant or exclusively distributed in different altitudes. At the Serra do Tapirapeco, sampling at four different elevations up to 1200 m, we only obtained 40 individuals divided in four species, and there was no clear relation to altitude. Most of the new species were found at mid and high altitude sites, while species from lower altitude sites represented widespread species. The comparison with other neotropical spiders inventories highlights the high diversity recorded at Pico da Neblina, which could be assigned to the large environmental variation covered in this work and to the sampling of high-altitude environments. Inventories in the Andean region and other information in the literature also seem to support the association of Chrysometa with high altitude environments.

Comparative phylogeography of Trypanosoma cruzi TCIIc: New hosts, association with terrestrial ecotopes, and spatial clustering

We characterized 28 new isolates of Trypanosoma cruzi IIc (TCIIc) of mammals and triatomines from Northern to Southern Brazil, confirming the widespread distribution of this lineage. Phylogenetic analyses using cytochrome b and SSU rDNA sequences clearly separated TCIIc from TCIIa according to terrestrial and arboreal ecotopes of their preferential mammalian hosts and vectors. TCIIc was more closely related to TCIId/e, followed by TCIIa, and separated by large distances from TCIIb and TCI. Despite being indistinguishable by traditional genotyping and generally being assigned to Z3, we provide evidence that TCIIa from South America and TCIIa from North America correspond to independent lineages that circulate in distinct hosts and ecological niches. Armadillos, terrestrial didelphids and rodents, and domestic dogs were found infected by TCIIc in Brazil. We believe that, in Brazil, this is the first description of TCIIc from rodents and domestic dogs. Terrestrial triatomines of genera Panstrongylus and Triatoma were confirmed as vectors of TCIIc. Together, habitat, mammalian host and vector association corroborated the link between TCIIc and terrestrial transmission cycles/ecological niches. Analysis of ITS1 rDNA sequences disclosed clusters of TCIIc isolates in accordance with their geographic origin, independent of their host species. (C) 2009 Elsevier B.V. All rights reserved.

Genes of cathepsin L-like proteases in Trypanosoma rangeli isolates: Markers for diagnosis, genotyping and phylogenetic relationships

We have sequenced genes encoding cathepsin L-like (CatL-like) cysteine proteases from isolates of Trypanosoma rangeli from humans, wild mammals and Rhodnius species of Central and South America. Phylogenetic trees of sequences encoding mature CatL-like enzymes of T rangeli and homologous genes from other trypanosomes, Leishmania spp. and bodonids positioned sequences of T rangeli (rangelipain) closest to T cruzi (cruzipain). Phylogenetic tree of kinetoplastids based on sequences of CatL-like was totally congruent with those derived from SSU rRNA and gGAPDH genes. Analysis of sequences from the CatL-like catalytic domains of 17 isolates representative of the overall phylogenetic diversity and geographical range of T rangeli supported all the lineages (A-D) previously defined using ribosomal and spliced leader genes. Comparison of the proteolytic activities of T rangeli isolates revealed heterogeneous banding profiles of cysteine proteases in gelatin gels, with differences even among isolates of the same lineage. CatL-like sequences proved to be excellent targets for diagnosis and genotyping of T rangeli by PCR. Data from CatL-like encoding genes agreed with results from previous studies of kDNA markers, and ribosomal and spliced leader genes, thereby corroborating clonal evolution, independent transmission cycles and the divergence of T rangeli lineages associated with sympatric species of Rhodnius. (c) 2009 Elsevier B.V. All rights reserved.

Phylogenetic, morphological and behavioural analyses support host switching of Trypanosoma (Herpetosoma) lewisi from domestic rats to primates

We characterized four Brazilian trypanosomes isolated from domestic rats and three from captive nonhuman primates that were morphologically similar to T. lewisi, a considered non-pathogenic species restricted to rodents and transmitted by fleas, despite its potential pathogenicity for infants. These isolates were identified as T. lewisi by barcoding using V7V8 SSU rDNA sequences. In inferred phylogenetic trees, all isolates clustered tightly with reference T. lewisi and T. lewisi-like trypanosomes from Europe, Asia and Africa and despite their high sequence conservation formed a homogeneous clade separate from other species of the subgenus T. (Herpetosoma). With the aim of clearly resolving the relationships between the Brazilian isolates from domestic rats and primates, we compared sequences from more polymorphic ITS rDNA. Results corroborated that isolates from Brazilian rats and monkeys were indeed of the same species and quite close to T. lewisi isolates of humans and rats from different geographical regions. Morphology of the monkey isolates and their behaviour in culture and in experimentally infected rats were also compatible with T. lewisi. However, infection with T. lewisi is rare among monkeys. We have examined more than 200 free-ranging and 160 captive monkeys and found only three infected individuals among the monkeys held in captivity. The findings of this work suggest that proximity of monkeys and infected rats and their exposure to infected fleas may be responsible for the host switching of T. Iewisi from their natural rodent species to primates. This and previous studies reporting T. lewisi in humans suggest that this trypanosome can cause sporadic and opportunistic fleaborne infection in primates. (C) 2010 Elsevier B.V. All rights reserved.

Animais medicinais: conhecimento e uso entre as populações ribeirinhas do rio Negro, Amazonas, Brasil

O estudo tem por objetivo documentar o uso de animais na medicina caseira entre ribeirinhos do rio Negro, Amazonas, Brasil. Os dados foram coletados por meio de 92 entrevistas e de observações sobre o conhecimento e as práticas cotidianas de uso de animais medicinais. Cerca de 60 espécies animais são conhecidas com propósitos medicinais. O conhecimento é bem distribuído entre os sexos (homens e mulheres) e entre localidades (urbano e rural). O uso de animais medicinais está imerso em conceitos etiológicos e envolve uma complexa visão cosmológica do processo de cura. O êxodo rural e o acesso facilitado à medicina ocidental podem promover a perda dos conhecimentos tradicionais, o que pode ser mitigado através da valorização e da transmissão desses saberes às futuras gerações.

Entre tradições e modernidade: conhecimento ecológico local, conflitos de pesca e manejo pesqueiro no rio Negro, Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Origins of sequence diversity in the malaria vaccine candidate merozoite surface protein-2 (MSP-2) in Amazonian isolates of Plasmodium falciparum

The recent evolution of Plasmodium falciparum is at odds with the extensive polymorphism found in most genes coding for antigens. Here, we examined the patterns and putative mechanisms of sequence diversification in the merozoite surface protein-2 (MSP-2), a major malarial repetitive surface antigen. We compared the msp-2 gene sequences from closely related clones derived from sympatric parasite isolates from Brazilian Amazonia and used microsatellite typing to examine, in these same clones, the haplotype background of chromosome 2, where msp-2 is located. We found examples of msp-2 sequence rearrangements putatively created by nonreciprocal recombinational events, such as replication slippage and gene conversion, while maintaining the chromosome haplotype. We conclude that these nonreciprocal recombination events may represent a major source of antigenic diversity in MSP-2 in P falciparum populations with low rates of classical meiotic recombination. (c) 2006 Elsevier B.V. All rights reserved.

Rural property size drives patterns of upland and riparian forest retention in a tropical deforestation frontier

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Evolucao materno-infantil da contaminacao por mercurio: Estudo em duas populacoes indigenas brasileiras

Considering the severity of Hg intoxication to human health and the frequent use of the metal in gold prespection on Brazilian Amazonia, an extensive project in being developed in order to quantify such conditions in two indigenous Kayapo populations, inhabitants of South of Para State, Gorotire and Djudjetikitire. This paper reports evolution of this contamination on mothers and babies. During puerperium, it was registered both an important decline on dosimetric values observed before in pregnancy and the occurrence of expressive levels in the babies. Such data are discussed based on contemporary medical-biologic conceptions, since it raises conceptual review of the matter.

Análise da sustentabilidade na produção familiar no Sudeste Paraense: o caso dos produtores de leite do Município de Rio Maria

Neste trabalho é feita uma análise da sustentabilidade da pecuária leiteira na agricultura familiar, decorrente do rápido processo de degradação das pastagens formadas em área de terra firme, numa região de fronteira da Amazônia brasileira. A pesquisa foi realizada no Município de Rio Maria, Sudeste Paraense, sendo este um dos Municípios do Pará reconhecido internacionalmente pelo alto índice de conflitos fundiários. Foram entrevistadas 55 unidades de produção familiar, nos Projetos de Assentamentos Itaipavas 126, Barra Mansa, Mata Azul, Fazenda São Roque e Vale da Serra que sobrevivem, especificamente, da pecuária leiteira, que foram entrevistados nos meses de julho a agosto de 2002. A escolha das propriedades foi intencional, e constitui-se na identificação da renda da pecuária (venda do leite e reses), bem como, uma análise das técnicas utilizadas pelos pequenos produtores, no manejo das pastagens, do rebanho para garantir a sustentabilidade da unidade produtiva. Essa análise permitiu identificar através dos indicadores socioeconômicos que, embora a pecuária seja considerada uma atividade de baixo risco, economicamente viável para a Amazônia, entre os pequenos produtores, torna-se uma atividade insustentável, posto que, o processo de degradação das pastagens inicia-se a partir de três a cinco anos, sem, no entanto, permitir que as unidades de produção poupem recursos para renovação ou recuperação. A renda sustentável da atividade de pecuária leiteira sendo muito baixa em relação à renda obtida logo na fase inicial da atividade desestimula a adoção de práticas mais sustentáveis. A tendência declinante da produtividade das pastagens, com leves acréscimos decorrentes das queimadas e de controle da juquira tem sido compensadas com a incorporação de novas áreas de pastagens. O esgotamento de estoques de reservas florestais tende levar ao colapso da atividade, a despeito da existência de mercado para carne e leite, as práticas de recuperação não são adotadas. Considerando uma taxa de depreciação de pastagens de 10% ao ano e uma taxa de juros de 15% ao ano, do lucro líquido obtido os proprietários deveriam investir pelo menos 40% para garantir a sustentabilidade das pastagens ao final de dez anos. Verifica-se que a pecuária leiteira da agricultura familiar está sendo feita com a contínua drenagem dos recursos naturais, sem a devida compensação no preço de venda desses produtos (leite e carne). Espera-se que estes resultados possam contribuir para definir políticas públicas, com medidas concretas para os pequenos produtores de leite, no sentido de garantir renovação/recuperação das pastagens degradadas, visto que, são estes produtores os responsáveis por grande parte do desequilíbrio ecológico do ecossistema no Sudeste Paraense. Entre os pequenos criadores de gado não há necessidade de financiamento para contínua aquisição do gado, pois todos os proprietários já possuem rebanho acima da capacidade das pastagens. Nesse caso, seria necessária capacitação do produtor capacitação do produtor, para manejo adequado do pasto e do rebanho e financiamentos voltados para recuperação das pastagens degradadas. Não existe entre os produtores um espírito de conservação, mas sim uma ansiedade em aumentar o rebanho e as pastagens.

Lanfrediella amphicirrus gen. nov. sp. nov. Nematotaeniidae (Cestoda: Cyclophyllidea), a tapeworm parasite of Rhinella marina (Linnaeus, 1758) (Amphibia: Bufonidae)

The family Nematotaeniidae, tapeworms commonly found in the small intestines of amphibians and reptiles, includes 27 recognised species distributed among four genera: Bitegmen Jones, Cylindrotaenia Jewell, Distoichometra Dickey and Nematotaenia Lühe. The taxonomy of these cestodes is poorly defined, due in part to the difficulties of observing many anatomical traits. This study presents and describes a new genus and species of nematotaeniid parasite found in cane toads (Rhinella marina) from eastern Brazilian Amazonia. The cestodes were collected during the necropsy of 20 hosts captured in the urban area of Belém, Pará. The specimens were fixed and processed for light microscopy, scanning electron microscopy (SEM) and three-dimensional (3D) reconstruction. Samples were also collected for molecular analyses. The specimens presented a cylindrical body, two testes and paruterine organs. However, they could not be allocated to any of the four existing nematotaeniid genera due to the presence of two each of dorsal compact medullary testes, cirri, cirrus pouches, genital pores, ovaries and vitelline glands per mature segment. Lanfrediella amphicirrus gen. nov. sp. nov. is the first nematotaeniid studied using Historesin analysis, SEM and 3D reconstruction, and it is the second taxon for which molecular data have been deposited in GenBank.