Mechanical properties and bioactive surface modification via alkali-heat treatment of a porous ti-18Nb-4Sn alloy for biomedical applications

A porous Ti–18 at.%Nb–4 at.%Sn (hereafter, Ti–18Nb–4Sn) alloy was prepared by powder metallurgy. The porous structures were examined by scanning electron microscopy and the phase constituents were analysed by X-ray diffraction. Mechanical properties of the porous alloy were investigated using a compressive test. To enhance the bioactivity of the alloy surface, alkali-heat treatment was used to modify the surface. The bioactivity of the pre-treated alloy sample was investigated using a biomimetic process by soaking the sample into simulated body fluid (SBF). Results indicate that the elastic modulus and plateau stress of the porous Ti–18Nb–4Sn alloy decrease with decreasing relative density. The mechanical properties of the porous alloy can be tailored to match those of human bone. After soaking in SBF for 7 days, a hydroxyapatite layer formed on the surface of the pre-treated porous Ti–18Nb–4Sn alloy. The pre-treated porous Ti–18Nb–4Sn alloy therefore has the potential to be a bioactive implant material.

Mechanical properties of titanium foam for biomedical applications

Understanding the mechanical behaviour of pure titanium (Ti) foam is crucial for the design and development of Ti foam-based load-bearing implants. In this work, pure titanium foam is fabricated by a powder metallurgical process using the space-holder technique with a spacer size of 500 to 800 µm. Experimental data from static compression testing on the Ti foam are presented. The application of theoretical formulae to predict Young's modulus and yield strength of titanium foams is also discussed. A foam with 63% porosity, 87 ± 5 MPa yield strength, and 6.5 ± 1.3 GPa Young's modulus is found to be appropriate for a number of dental and orthopaedic applications.

Preparation of bioactive porous Ti-Sn-Nb alloy for biomedical applications

A porous Ti-16Sn-4Nb alloy with an average pore size of 300 µm and porosity of 60 % was prepared by powder metallurgy, and a bone-like apatite coating was obtained by soaking the samples in a concentrated simulated body fluid (lOx SBF). The changes of the microstructure and composition on the surface with soaking time were investigated by using X-ray diffractometry (XRD), and scanning electron microscopy equipped with energy dispersive spectroscopy (SEM-EDS), The bone-like apatite granules started to deposit throughout the porous Ti alloy foam aner 1 h soaking, and the number of granules increased with the increase of the soaking time. A uniform bone-like apatite layer covered the entire surface of the sample after soaking in Ihe lOx SBF for 6h. The Ti-16Sn-4Nb foam showed a good bioactivity after a thermochemical process and soaking into a 1Ox SBF.

Processing and mechanical properties of porous Ti-Mo alloy for biomedical applications

Titanium and some of its alloys are well accepted as load-bearing implant materials due to their excellent mechanical properties, superior corrosion resistance, and outstanding biocompatibility. However, solid implant materials may suffer from the problems of adverse tissue reaction, biomechanical mismatch and lack of new bone tissue ingrowth ability. In the present study, porous titanium-molybdenum (Ti-Mo) alloy was fabricated by the space-holding sintering method. The pore size, pore shape and porosity can be controlled through choosing appropriate space-holding particle materials. Electron scanning microscopy (SEM) was used for the characterisation of the porous Ti-Mo alloy. The mechanical properties of the porous Ti-Mo alloy samples were investigated by compressive tests. Results indicated that the porous Ti-Mo alloy provides promising potential for new implant materials with new bone tissue ingrowth ability and mechanical properties mimicking those of natural bone.

Influence of calcium ion deposition on apatite-inducing ability of porous titanium for biomedical applications

In the present study, the influence of calcium ion deposition on the apatite-inducing ability of porous titanium(Ti) was investigated in a modified simulated body fluid (m-SBF). Calcium hydroxide (Ca(OH)₂) solutions with five degrees of saturation were used to hydrothermally deposit Ca ions on porous Ti with a porosity of 80%. Apatite-inducing ability of the Ca-ion-deposited porous Ti was evaluated by soaking them in m-SBF for up to 14 days. Scanning electron microscopy (SEM) and X-ray diffractometry (XRD) confirmed that a thin layer of calcium titanate (CaTiO₃)/calcium oxide (CaO) mixture with a nanostructured porous network was produced on porous Ti substrates after hydrothermal treatment at 200 °C for 8 h. X-ray photoelectron spectroscopy results demonstrated that the content of the Ca ions deposited on Ti and the thickness of the CaTiO₃/CaO layer increased with increasing saturation degree of the Ca(OH)₂ solution. The thickest (over 10 nm) CaTiO₃/CaO layer with the highest Ca content was achieved on the Ti treated in an oversaturated Ca(OH)₂ solution (0.2 M). SEM, XRD, transmission electron microscopy and Fourier transformed infrared spectroscopy analysis indicated that the porous Ti samples deposited with the highest content of Ca ions exhibited the best apatite-inducing ability, producing a dense and complete carbonated apatite coating after a 14 day soaking in m-SBF. The present study illustrated the validity of using Ca ion deposition as a pre-treatment to endow desirable apatite-inducing ability of porous Ti for bone tissue engineering applications.

Porous TiNbZr alloy scaffolds for biomedical applications

In the present study, porous Ti–10Nb–10Zr alloy scaffolds with different porosities were successfully fabricated by a ‘‘space-holder” sintering method. By the addition of biocompatible alloying elements the porous TiNbZr scaffolds achieved significantly higher strength than unalloyed Ti scaffolds of the same porosity. In particular, the porous TiNbZr alloy with 59% porosity exhibited an elastic modulus and plateau stress of 5.6 GPa and 137 MPa, respectively. The porous alloys exhibited excellent ductility during compression tests and the deformation mechanism is mainly governed by bending and buckling of the struts. Cell cultures revealed that SaOS2 osteoblast-like cells grew on the surface and inside the pores and showed good spreading. Cell viability for the porous scaffold was three times higher than the solid counterpart. The present study has demonstrated that the porous TiNbZr alloy scaffolds are promising scaffold biomaterials for
bone tissue engineering by virtue of their appropriate mechanical properties, highly porous structure and excellent biocompatibility.

Novel metal structures through powder metallurgy for biomedical applications

Biocompatible porous Ti-16Sn-4Nb alloys were synthesised in quest of a novel tissue engineering biomaterial for bone regeneration. The alloys were prepared from elemental powders via mechanical alloying followed by space-holder sintering. The effects of ball milling variables on the characteristics and mechanical properties of bulk and porous Ti-16Sn-4Nb alloy have been investigated.

Porous Ti-Nb-Zr alloy through powder metallurgy for biomedical applications

This work investigated the structure and properties relationship, surface modification, biocompatibility and bioactivity of a porous Ti-Nb-Zr alloy. The porous alloy exhibited inter-connected porous structure, good biocompatibility and high mechanical strength with an elastic modulus close to that of bone. Porous Ti-Nb-Zr alloys are thus promising biomaterials for hard tissue replacement.

Porous shape memory alloy scaffolds for biomedical applications : a review

The interest in using porous shape memory alloy (SMA) scaffolds as implant materials has been growing in recent years due to the combination of their unique mechanical and functional properties, i.e. shape memory effect and superelasticity, low elastic modulus combined with new bone tissue ingrowth ability and vasculariszation. These attractive properties are of great benefit to the healing process for implant applications. This paper reviews current state-of-the art on the processing, porous characteristics and mechanical properties of porous SMAs for biomedical applications, with special focus on the most widely used SMA nickel-titanium (NiTi), including (i) microstructural features, mechanical and functional properties of NiTi SMAs; (ii) main processing methods for the fabrication of porous NiTi SMAs and their mechanical properties and (iii) new-generation Ni-free, biocompatible porous SMA scaffolds.

Titanium-based shape memory alloys and scaffolds for biomedical applications

Porous Ti-50.5Ni shape memory alloys with different porosities were produced using a space-holder sintering method. A new Ni-free Ti-based shape memory alloy, Ti-18Nb-5Mo-5Sn, was developed for potential biomedical applications, and a novel one-step hydrothermal process was applied to produce hydroxyapatite coatings on the surface of Ti alloy.

Protein fiber particles for biomedical applications

Ultrafine protein particles have been fabricated from natural fibres, such as silk and wool. Our studies suggested that particles could be used for fabricating tough macro-porous composites scaffolds for tissue engineering. They are also efficient for reversible binding of metal ions. We are currently analyzing the sorption properties, biocompatibility and biodegradability of a range of particles to evaluate possibility for biomedical and healthcare applications.

Carbon nanotubes for biological and biomedical applications

Ever since the discovery of carbon nanotubes, researchers have been exploring their potential in biological and biomedical applications. The recent expansion and availability of chemical modification and bio-functionalization methods have made it possible to generate a new class of bioactive carbon nanotubes which are conjugated with proteins, carbohydrates, or nucleic acids. The modification of a carbon nanotube on a molecular level using biological molecules is essentially an example of the 'bottom-up' fabrication principle of bionanotechnology. The availability of these biomodified carbon nanotube constructs opens up an entire new and exciting research direction in the field of chemical biology, finally aiming to target and to alter the cell's behaviour at the subcellular or molecular level. This review covers the latest advances of bio-functionalized carbon nanotubes with an emphasis on the development of functional biological nano-interfaces. Topics that are discussed herewith include methods for biomodification of carbon nanotubes, the development of hybrid systems of carbon nanotubes and biomolecules for bioelectronics, and carbon nanotubes as transporters for a specific delivery of peptides and/or genetic material to cells. All of these current research topics aim at translating these biotechnology modified nanotubes into potential novel therapeutic approaches.

Microstructural characterization and mechanical properties of Mg-Zr-Ca alloys prepared by hot-extrusion for biomedical applications

This paper investigated the microstructural characterization and mechanical properties of Mg-Zr-Ca alloys prepared by hot-extrusion for potential use in biomedical applications. Mg-Zr-Ca alloys were fabricated by commercial pure Mg (99.9%), Ca (99.9%), and master Mg-33% Zr alloy (mass%). The microstructural characterization of the hot-extruded Mg-Zr-Ca alloys was examined by X-ray diffraction analysis and optical microscopy, and the mechanical properties were determined from tensile tests. The experimental results indicate that the hot-extruded Mg-Zr-Ca alloys with 1 mass% Ca are composed of one single phase and those alloys with 2 mass% Ca consist of both Mg2Ca and α phase. The hot-extruded Mg-Zr-Ca alloys exhibit equiaxed granular microstructures and the hot-extrusion process can effectively increase both the tensile strength and ductility of Mg-Zr-Ca alloys. The hot-extruded Mg-1Zr-1Ca alloy (mass%) exhibits the highest strength and best ductility among all the alloys, and has much higher strength than the human bone, suggesting that it has a great potential to be a good candidate for biomedical application.

Compressive properties of hot-rolled Mg-Zr-Ca alloys for biomedical applications

This paper investigated the microstructures and compressive properties of hot-rolled Mg-Zr-Ca alloys for biomedical applications. The microstructures of the Mg-Zr-Ca alloys were examined by X-ray diffraction analysis and optical microscopy, and the compressive properties were determined from compressive tests. The experimental results indicate that the hot-rolled Mg-Zr-Ca alloys with 1% Ca are composed of one single α phase and those alloys with 2% Ca consist of both Mg2Ca and α phase. The hot-rolled Mg-Zr-Ca alloys exhibit typical elongated microstructures with obvious fibrous stripe, and have much higher compressive strength and lower compressive modulus than pure Mg. All the studied alloys have much higher compressive yield strength than the human bone (90~140 MPa) and comparable modulus with the human bone, suggesting that they have a great potential to be good candidates for biomedical applications.

Ribosome-inactivating proteins : current status and biomedical applications

Ribosome-inactivating proteins (RIPs) are mainly present in plants and function to inhibit protein synthesis through the removal of adenine residues from eukaryotic ribosomal RNA (rRNA). They are broadly classified into two groups: type I and type II. Type I RIPs are a diverse family of proteins comprising a single polypeptide chain, whereas type II RIPs are heterodimeric glycoproteins comprising an A-chain (functionally equivalent to a type I RIP) linked via a disulphide bond to a B chain, mediating cell entry. In this review, we describe common type I and type II RIPs, their diverse biological functions, mechanism of cell entry, stability in plasma and antigenicity. We end with a discussion of promising applications for RIPs in biomedicine.