Structural studies of the hen's egg lipovitellins. NMR and fluorescence investigations

The pH dependent reversible association-dissociation reaction of α- and β-lipovitellins from egg yolk has been studied by 1H NMR and fluorescence probe methods. Increased mobility of the choline methyl groups has been demonstrated on dissociation. The lipid methylene resonance of β-lipovitellin shows clear doublet character suggesting that the fatty acid chains exist in distinct environments. The high field component increases with temperature but is suppressed on treatment with pronase, suggesting a significant role for proteins in maintaining the differences in lipid environments. 1-Anilino-8-naphthalene sulfonate has been shown to bind less effectively to the monomeric lipovitellins. This is in agreement with earlier results suggesting that dissociation may be accompanied by increased hydration and conformational changes.

Physicochemical studies on the gelation of hen egg yolk; separation of gelling protein components from yolk plasma

A study of the component(s) in egg yolk responsible for gelation of yolk on freezing and thawing has shown that granule-free yolk plasma, obtained by high-speed centrifugation of yolk, has the capacity to gel. As with the whole yolk, gelation of yolk plasma on freezing and thawing could be inhibited by additives such as sugars, sodium chloride, proteolytic enzymes, and phospholipase-A. Phospholipase-C, which induces gelation of whole yolk at room temperature, has a similar effect on yolk plasma. Yolk plasma has been separated into aggregating (gelling) and soluble fractions by delipidation, using formic acid. Each of these fractions consists of three or four protein components, as observed by gel filtration, ultracentrifugation, and agar electrophoresis. The proteins are glycoproteins and contain bound hexoses, hexosamine, and sialic acid. The gelation of yolk has been attributed to the interactions between protein molecules following disruption of lipid-protein bonds.

Physicochemical studies on the gelation of hen's egg yolk: Delipidation of yolk plasma by treatment with phospholipase-C and extraction with solvents

A method for the delipidation of egg yolk plasma using phospholipase-C, n-heptane, and 1-butanol has been described. An aggregating protein fraction and a soluble protein fraction were separated by the action of phospholipase-C. The aggregating protein fraction freed of most of the lipids by treatment with n-heptane and 1-butanol was shown to be the apolipoproteins of yolk plasma, whereas the soluble proteins were identified as the livetins. Carbohydrate and the N-terminal amino acid analysis of these protein fractions are reported. A comparison of these protein fractions with the corresponding fractions obtained by formic acid delipidation of yolk plasma has been made. The gelation of yolk plasma by the action of phospholipase-C has been interpreted as an aggregation of lipoproteins caused by ionic interactions. The role of lecithin in maintaining the structural integrity of lipoproteins has been discussed.

A simplified method for the purification of riboflavin-binding protein from hen's egg yolk

A simple and efficient procedure for the purification of the riboflavin-binding protein from hen's egg yolk is described. This method involves the removal by exclusion of lipoproteins and subsequent fractionation of soluble yolk proteins held on a DEAE-cellulose column by a salt gradient which is followed by purification by gel filtration on Sephadex G-100. The protein thus isolated is homogeneous by various physicoehemical, immunological, and functional criteria.

Io. gottl. heineccii, iureconsulti quondam celeberrimi : recitationes in elementa iuris civilis secundum ordinem institutionum, accedunt io. christ. gottl. heineccii ... commentarius de vita, fatis ac scriptis b. parentis, auctior emendatior christiani thomasii, delineatio historiae juris nec non burc. gottelf. struvii, bibliotheca juris selectissima cum notis hen. contelmanni, ubi praestantiora iurisconsultorum opera ad nostra usque tempora referuntur

Título Uniforme : Corpus Juris Civilis. Institutas.

Study on the biomass yield of Duckweed (Lemna minor) (L.) in hydroponic cultures containing different concentrations of aquaeous extract of chicken manure

The biomass yields of duck week (Lemna minor(L) was monitored in hydroponic media prepared by variously extracting 0.50, 1.00 and 2.00g of dried chicken manure per liter of city water (tap water) supply. The culture media consisting of aqueous extract of the various manure treatments were made up to 12 liters in all cases with tap water as control. Plastic baths of 25 liters capacity with 0.71 super(m2) surface area were used as culture facility. Each bath was stocked at a density of 30g super(m-2) with fresh weed samples (i.e 21.30g/bath). Maximum yields were obtained at all treatment levels and control on day 3 and based on the highest yield of 0.37gm super(-2)d super(-1) (dry matter) obtained at 1.00gL manure treatment which was however not significantly higher (P>0.05) than the 0.36gm super(-2)d super(-1) (dry matter) at 0.05gl super(-1) media manure content, an average manure level of 0.75l super(-1) was selected and used to determine the operational plant density. Thus fresh weights of 30 to 300gm super(-2) was grown in triplicate at 30g intervals for a period of 3 days. A regression equation of Y=2.6720+0.0021x with a corresponding maximum density or operational plant density of 266gm super(-2) and yield of 0.98gm super(-2), d super(-1) (dry matter) were obtained. Further growth trials were carried out at the operational density and manure levels of 0.50, 0.75, 1.00, 1.25, 1.50, 1.75 and 2.00gl super(-1) media manure concentration giving a significantly higher yield (P<0.05) of 17gm super(-2), d super(-1) (dry matter). This yield was however doubled to between 2.21 and 2.24gm super(-2) d super(-1) (equivalent to 7.96 to 8.06mt.ha-1, Yr-1 dry matter on extrapolation) if 25% and 75% respectively of the total weed cover were harvested daily within the experimental period. The role of some dissolved plant nutrients (DPN) were also discussed

Pig dung as pond manure: effect on water quality, pond productivity and growth of carps in polyculture system

Pig dung was used as manure at 18 and 36 t/ha/year in carp polyculture (without supplementary feeding) for 270 days. It was observed that pig dung at both the levels did not degrade the physico-chemical properties (pH, dissolved oxygen and alkalinity) of water. The nutrient (phosphates and nitrates) level of water was higher in manured ponds than control ponds (no manuring, only supplementary feeding). Further, plankton levels (phyto- and zooplankton) were also significantly higher in manured ponds. The growth of Catla catla and Labeo rohita was significantly more in manured ponds than in control ponds. Growth of Cirrhinus cirrhosus and Cyprinus carpio was significantly more in ponds manured with pig dung at 18 t/ha/year than in control ponds and the growth of Ctenopharyngodon idellus was significantly more in control than in manured ponds.

Baines Pool Hen Pike caught 7.3.1950

A fisherman with a hen pike caught on the 7.3.1950 at Baines Pool, North West of England, UK. This photo is part of a photo album that includes pictures from 1935 to 1954.

Oyster mushroom waste as manure in fish culture: a preliminary study

In an attempt to recycle the waste substrates of the oyster-mushroom crop, tanks were stocked with seed of Indian major carp Cirrhinus mrigala at the rate of 600,000/ha and waste substrate was applied at weekly interval at 0, 50, 100, 150, 200 and 250 g/tank. Oyster mushroom waste not only provided highly nutritive colonised detritus to the fish as direct feed, but also produced rich plankton in the tank. In waste treated tanks, production was better than in the control in 150, 200 and 250 g/tank treatment suggesting the possibility of fish-oyster mushroom integration.

Effects of black Bengal goat manure on growth and production of Genetically Improved Farmed Tilapia (GIFT) starin [sic], Oreochromis niloticus

In a goat-tilapia integrated farming system, the effect of Black Bengal goat manure on the growth and production of Oreochromis niloticus was studied at the Freshwater Substation, Shantahar, Bogra for 4.5 months. The stocking density used in three treatments were, 200 goats and 15,000 GIFT strain/ha (T1); 300 goats and 15,000 GIFT strain/ha (T2); and only 15,000 GIFT strain/ha (T3). The initial individual total length and weight of stocked tilapia were 7.6 cm and 11.34 g, respectively. Twelve ponds each having an area of 40 square meters were used for this trail [sic]. On one side of each pond goat shed was constructed and the space allocated for each goat was 0.75 m x 1.5 m. Newly weaned black Bengal goats of average weight 8.45 kg was used in the trial. Every morning the goats manure was swiped out in ponds through the fixed opening of bamboo made floor of goat shed. Water depth of the ponds was maintained at 0.75 m. The highest fish production was in treatment 1 (1,750 kg/ha) followed by treatment 2 (1,455 kg/ha) and treatment 3 (621 kg/ha). The difference in individual goat weight was not significant (P>0.05) among treatments 1 and 2.