Biochemical Engineering Symposium Proceedings (June 4, 1971)

This report presents the proceedings of the Biochemical Engineering Symposium held at Kansas State University, June 4, 1971. Since most of the papers will be published elsewhere, only very brief papers are included here. Moreover, several of the projects are still in progress at this time. Request for additional information on projects conducted at the University of Nebraska should be directed to Dr. Peter J. Reilly and for Kansas State University to Dr. L. E. Erickson. ContentsChao, Chih-Cheng, University of Nebraska, "Symbiotic Growth of Actobacter suboxydans and Saccharomyces carlsbergensis in a Chemostat" S.Y. Chiu, Kansas State University, "Model Identification in Mixed Populations Using Continuous Culture Data" Shinji Goto, University of Nebraska, "Symbiotic Growth of Bacteria and Blue Green Algae in a Chemostat" I.C. Kao, Kansas State University, "ATP as a Parameter of Mixed Culture Interaction" Indravadan R. Kothari, University of Nebraska, "Growth of Single Cells of Schizocaccharomyces pombe under Nutrient Limitation" G.C.Y. Chu, Kansas State University, "Experimental Optimization of Biological Waste Treatment Processes" Mark Young, University of Nebraska, "Aerobic Fermentation of Paunch Liquor" P.S. Shah, Kansas State University, "Optimal Control of Growth Processes"

Proceedings of the Third Biochemical Engineering Symposium (April 28, 1973)

This report presents the proceedings of the Biochemical Engineering Symposium held at Kansas State University, April 28, 1973. Since a number of the contributions will be published in detail elsewhere, only brief summaries of each contribution are included here. Requests for additional information on projects conducted at The University of Nebraska should be directed to Dr. Peter J. Reilly, and those at Kansas State University to the editors. ContentsKenneth J. Jacobson, Andrew H.C. Chan, and Raymond C. Eliason, "Properties and Utilization of Small Particulates in Cattle Manure" Cady R. Engler and James S. Yohn, "Protein from Manure" Robert J. Williams, "Kinetics of Sucrose Inversion Using Invertase Immobilized on Hollow Fibers of Cellulose Acetate" David F. Aldis and Thomas A. Carlisle, "Study of a Triiodide-Resin Complex Disinfection System" John C. Heydweiller, "Modeling and Analysis of Symbiotic Growth" Kenneth J. Jacobson, "Synchronized Growth of the Blue Green Alga Microcystis aeruginosa" Clarence C. Y. Ron arui Lincoln L. S. Yang, "Computer Modeling of the Reductive Pentose Phosphate Cycle" Ming-ching T. Kuo, "Application of a Parallel Biochemical Oxidation Kinetic Model to the Design of an Activated Sludge System Including a Primary Clarifier" Prakash N. Mishra, "Optimal Synthesis of Water Renovation Systems"

Second Annual Kansas State–Nebraska Biochemical Engineering Symposium (April 29, 1972)

This booklet contains abstracts of papers presented at a biochemical engineering symposium conducted at the University of Nebraska-Lincoln on April 29, 1972. This was the second annual symposium on this subject, the first having been held at Kansas State University on June 4, 1971. It is expected that future symposia will alternate between the two campuses. ContentsS.H. Lin, Kansas State University, "Enzyme Reaction in a Tubular Reactor with Laminar Flow" Gregory C. Martin, University of Nebraska, "Estimation of Parameters in Population Models for Schizosaccharomyces pombe from Chemostat Data" Jaiprakash S. Shastry and Prakash N. Mishra, Kansas State University, "Immobilized Enzymes: Analysis of Ultrafiltration Reactors" Mark D. Young, University of Nebraska, "Modelling Unsteady-State Two-Species Data Using Ramkrishna's Staling Model" G.C.Y. Chu, Kansas State University, "Optimization of Step Aeration Waste Treatment Systems Using EVOP" Shinji Goto, University of Nebraska, "Growth of the Blue-Green Alga Microcytis aeruginosa under Defined Conditions" Prakash N. Mishra and Thomas M.C. Kuo, Kansas State University, "Digital Computer Simulation of the Activated Sludge System: Effect of Primary Clarifier on System Performance" Mark D. Young, University of Nebraska, "Aerobic Fermentation of Paunch Liquor"

Proceedings of the Fifth Biochemical Engineering Symposium

This report presents the proceedings of the Biochemical Engineering Symposium held at Kansas State University, April 26, 1975. Since a number of the contributions will be published in detail elsewhere, only brief summaries of each contribution are included here. Requests for additional information on projects conducted at Iowa State University should be directed to Dr. Peter J. Reilly, and those at Kansas State University to the editors. Contents"Enzymatic Breakdown of Hemicellulose," Alfred R. Fratzke, Iowa State University "Biochemical Aspects of Hydrocarbon Uptake in Hydrocarbon Fermentations," Tadaatsu Nakahara, Kansas State University "Optimal Concentration Profiles for Bifunctional Catalysts with Langmuir-Hinshelwood Kinetics and Varying Effectiveness Factors," Ho Nam Chang, Iowa State University "Single Cell Protein Production from Hydrocarbons in Tower Systems," J. R. Gutierrez, Kansas State University "Effect of Temperature and pH on the Stability and Activity of Immobilized Glucoamylase and Glucose Isomerase," Gene K. Lee, Iowa State University "Oxygen Transfer in a Tower System with Two Liquid Phases," G. T. MacLean, Kansas State University "Continuous Production of Glucose from Dextrin by Glucoamylase Immlobilized on Porous Silica," Douglas D. Lee, Iowa State University

Proceedings of the Fourth Annual Kansas State–Nebraska Biochemical Engineering Symposium

The symposium whose papers are abstracted here was the fourth in a series held alternately at Kansas State University and the University of Nebraska–Lincoln. Requests for further information on projects conducted at Kansas State should be directed to Professor L.E. Erickson and on those at Nebraska to the editor. ContentsJohn C. Heydweiller, "Estimating Sedimentation of Organisms in a Tower-Type Activated Sludge System" Raymond C. Eliason, "Properties and Utilization of Small Particulates in Cattle Manure" Kenneth H. Hsu, "Oxygen Transfer in Tower Systems with Motionless Mixers" Raymond C. Eliason, "Hydrolysis of Sucrose by 20 Invertase Immobilized on Hollow Fibers" Robert Shipman, "Single Cell Protein from Photosynthetic 26 Bacteria" Peter J. Reilly, "Stability of Commensalistic Systems"

Proceedings of the Sixth Annual Biochemical Engineering Symposium

This symposium is the sixth of an annual series conducted so that results of biochemical engineering research can be exchanged by the researchers who actually carry it out. The first four meetings were held alternately at Kansas State University and the University of Nebraska–Lincoln for attendees from those two schools. The fifth and sixth involved participants from Kansas State University and Iowa State University; this was the first meeting away from a university campus. Contents"Mathematical Model of Oxygen Transfer in Airlift Fermentors," Chester S. Ho, Kansas State University "Effect of Column Height on Oxygen Transfer in Airlift Systems," Mark E. Orazem, Kansas State University "Mixing Studies in an Oil-Water Airlift System with Motionless 15 Mixers", J. R. Gutierrez, Kansas State University "Purification and Properties of (3-Xylosidase," Gbekeloluwa B. Oguntimein, Iowa State University "Immobilization of Invertase to Cellulose with Cyanuric Chloride," William J. Smith, Iowa State University "Purification and Properties of Dextransucrase," Yah Eric Chen and Hossein Kaboli, Iowa State University "Properties of Immobilized (3-Amylase," Clarence C. Ron, Iowa State University

Proceedings of the Seventh Annual Biochemical Engineering Symposium

This is the seventh in a series of symposia devoted to talks by students on their biochemical engineering research. The first four meetings were held alternately at Kansas State University and the University of Nebraska–Lincoln, with participants from those two schools. The next two took place at Kansas State and then in conjunction with the 8lst American Institute of Chemical Engineers National Meeting in Kansas City, with attendees from Kansas State and Iowa State Universities. This meeting, at Iowa State, was the first to include participation from the University of Missouri–Columbia. Contents"Properties of Soluble and In:anoblized Dextransucrase," Hossein Kaboli and Yah Eric Chen, Iowa State University "Growth of Lipid-Producing Organisms on Formic and Acetic Acid-Containing Waste Waters," Lin-Chang Chiang, University of Missouri–Columbia "Design of an Automated Alkaline Copper Reducing Sugar Assay," Alfred R. Fratzke and James R. Frederick, Iowa State University "Determination of Oxygen Transfer Coefficients in Hydrocarbon Fermentations Using a Material Balance Method," Sarafin N. Sanchez and J. R. Gutierrez, Kansas State University "Oxygen Transfer Characteristics in One Stage and Two Stage Air-Lift Towers," Mark E. Orazem, Kansas State University "A Comparison of Biological Digestibility Tests for Cellulose," Dou-Houng Hwang, University of Missouri–Columbia "Mechanism of Enzymatic Hydrolysis of Cellulose," L. T. Fan, Yong-Hyun Lee, and Liang-Shih Fan, Kansas State University "Purification of Xylan-Hydrolyzing Enzymes," James R. Frederick, Alfred R. Fratzke, and Mary M. Frederick, Iowa State University "Cellulase Production from Bagasse and Pith," A. Ferrer, Y. Alroy, and I. Brito, Kansas State University

Proceedings of the Ninth Annual Biochemical Engineering Symposium

This report presents the proceedings of the Biochemical Engineering Symposium held at Kansas State University, April 28, 1979. Since a number of the contributions will be published in detail elsewhere, only brief reports of each contribution are included here. Requests for further information on work at Iowa State University should be directed to Dr. Peter J. Reilly; at Colorado State University to Drs. V. G. Murphy and A. R. Moreira, and at Kansas State University to Drs. L. T. Fan and L. E. Erickson. ContentProperties of a Homogeneous Xylobiohydrolase from Aspergillus niger, Mary M. Frederick, Iowa State University Kinetic Studies on the Enzymatic Hydrolysis of Cellulose–Absorption and Desorption of Cellulase onto Cellulose and the Behavior of Absorbed Cellulase, Yong-Hyun Lee and L. T. Fan, Kansas State University Properties of a Homogeneous Endo-Xylanase from Aspergillus niger, Ricardo Fournier A., Iowa State University Solid State Fermentation of Manure Fibers, D. C. Ulmer, Colorado State University Analysis and Consistency of Experimental Data for Microbial Growth on Renewable Resources, B. 0. Solomon, Kansas State University Biochemical Mechanisms of Enzyme Regulation, Frederick A. Blum, Colorado State University An Evaluation of Cellulose Pretreatments for Enzymatic Hydrolysis, David H. Beardmore, Kansas State University Use of Immobilized 8-Amylase/Glucoamylase Mixtures to Produce High Maltose Syrups, Carol G. Bohnenkamp, Iowa State University Effect of Viscosity on Bubble Behavior in an Airlift Fermentor, Vasanti Deshpande, Kansas State University

Proceedings of the Eighth Annual Biochemical Engineering Symposium

Contents"Effects of Swell-Shear Treatment on the Digestibility of Cellulosics", Dou-Houng Hwang, UMC "Application of Material and Energy Balance Regularities to Biomass Production from Cellulosic Substrates", Y.H. Lee, KSU "Immobilization of Aspergillus niger beta-Xylosidase", Gbekeloluwa B. Oguntimein, ISU "The Effect of the Major Structural Parameters of Cellulose on Enzymatic Hydrolysis", David H. Beardmore, Y.H. Lee, and L.T. Fan, KSU "Purification of a High Molecular Weight Hemicellulase", Ricardo A. Fournier, ISU "Aerobic Fermentation of Banana Pulp by Aspergillus Fumigatus", Stephen Lorbert, UMC "Purification and Properties of Two Very Small Xylanases", Chih-hen Kiang, ISU "Testing Theoretical Models for Cellulose Enzymatic Hydrolysis", Lin-Chang Chiang, UMC "Utilization of Material and Energy Balances in Hydrocarbon Fermentation", Alexis Ferrer, KSU "Purification of a Series of Closely Related Xylanases", Mary M. Frederick, ISU

Proceedings of the Twelfth Annual Biochemical Engineering Symposium

This work presents the proceedings of the twelfth symposium which was held at Kansas State University on April 24, 1982. Since a number of the contributions will be published in detail elsewhere, only brief reports are included here. Some of the reports describe current progress with respect to ongoing projects. Requests for further information should be directed to Dr. Peter Reilly at Iowa State University, Dr. V. G. Murphy at Colorado State University, Dr. Rakesh Bajpai at University of Missouri, Dr. Ed Clausen at University of Arkansas, Dr. L. T. Fan and Dr. L. E. Erickson at Kansas State University. ContentsA Kinetic Analysis of Oleaginous Yeast Fermentation by Candida curvata on Whey Permeate, B.D. Brown and K.H. Hsu, Iowa State University Kinetics of Biofouling in Simulated Water Distribution Systems Using CSTR, T.M. Prakash, University of Missouri Kinetics of Gas Production by C. acetobutylicum, Michael Doremus, Colorado State University Large Scale Production of Methane from Agricultural Residues, O.P. Doyle, G.C. Magruder, E.C. Clausen, and J.L. Gaddy, University of Arkansas The Optimal Process Design for Enzymatic Hydrolysis of Wheat Straw, M.M Gharpuray and L.T. Fan, Kansas State University Extractive Butanol Fermentation, Michael Sierks, Colorado State University Yields Associated with Ethyl Alcohol Production, M.D. Oner, Kansas State University Estimation of Growth Yield and Maintenance Parameters for Microbial Growth on Corn Dust, B.O. Solomon, Kansas State University Milling of Ensiled Corn, Andrzej Neryng, Iowa State University Protein Extraction from Alfalfa, Ravidranath Joshi, Colorado State University Analysis of Disaccharides by Capillary Gas Chromatography, Z.L. Nikolov, Iowa State University Characterization of High Viscosity Fermentations in Tower Fermentors, S.A. Patel and C.H. Lee, Kansas State University Utilization of Sugars in Sorghum Molasses by Clostridium acetobutylicum B. Hong, K.C. Shin, and L.T. Fan, Kansas State University

Proceedings of the Eleventh Annual Biochemical Engineering Symposium

This is the eleventh in a series of symposia devoted to talks by students on their biochemical engineering research. The first, third, fifth, and ninth were at Kansas State University in Manhattan; the second and fourth were at the University of Nebraska–Lincoln; the sixth was in Kansas City in conjunction with the 8lst American Institute of Chemical Engineers National Meeting; the seventh and the tenth were at Iowa State University in Ames; and the eighth was held at the University of Missouri–Columbia. ContentsPretreatment of Wheat Straw for Cellulose Hydrolysis, M. M. Gharpuray, Yong-Hyun Lee, and L. T. Fan, Kansas State University Sugar Production During Autohydrolysis of Wheat Straw, Robert A. Lewis, Colorado State University An Alkaline Copper Reagent for Use in Automated Analysis, Alfred R. Fratzke, Iowa State University Sugars Produced During Extrusion Processing of Corn, Ruth S. Korn, Colorado State University Characterization and Comparison of Renewable Energy Resources, Snehal A. Patel, Kansas State University Anaerobic Digestion of Alcohol Stillage, Laureen K. Binder, Colorado State University Estimation of Growth Yield and Maintenance Parameters, Bamidele 0. Solomon and Mehmet D. Oner, Kansas State University Immobilization of Glucoamylase Using TiCl4 and Organic Titanates, Robert E. Lesch, Iowa State University Solvent Toxicity in the Acetone-Butanol Fermentation, Jeanine M. Costa, Colorado State University

Proceedings of the 10th Annual Biochemical Engineering Symposium

This the tenth in a series of symposia devoted to talks by students on their biochemical engineering research. The first, third, fifth, and ninth were at Kansas State University in Manhattan, the second and fourth were at the University of Nebraska–Lincoln, the sixth was in Kansas City in conjunction with the 81st American Institute of Chemical Engineers National Meeting, the seventh was at Iowa State University in Ames, and the eighth was held at the University of Missouri–Columbia. Contents"Combined Autohydrolysis-Organosolv Pretreatment of Lignocellulosic Materials," Robert A. Lewis, Colorado State University "An Investigation of Cellulase Activity Assays," Minhhuong Nguyen, University of Missouri–Columbia "Action Pattern of a Xylobiohydrolase from Aspergillus niger," Mary M. Frederick, Iowa State University "Estimation of Heats of Combustion of Biomass from Elemental Analysis Using Available Electron Concepts," Snehal A. Patel, Kansas State University "Design of a Wheat Straw to Ethanol Conversion Facility," Michael M. Meagher, Colorado State University "Effects of Salt, Heat, and Physical Form on the Fermentation of Bananas," Carl Drewel, University of Missouri–Columbia "Gas Hold-up in the Downflow Section of a Split Cylinder Airlift Column," Vasanti Deshpande, Kansas State University "Measurement of Michaelis Constants for Soluble and Immobilized Glucoamylase," Robert A. Lesch, Iowa State University "Kinetics of Alkaline Oxidation and Degradation of Sugars," Alfred R. Fratzke, Iowa State University "Stability of Cereal Protein During Microbial Growth on Grain Dust," Bamidele O. Solomon, Kansas State University

Proceedings of the 13th Annual Biochemical Engineering Symposium

The symposium reported here was the thirteenth of a series devoted to talks by students on their biochemical engineering research. The first, third, fifth, ninth, and twelfth were at Kansas State University, the second and fourth were at the University of Nebraska–Lincoln, the sixth was in Kansas City and was hosted by Iowa State University, the seventh and tenth were at Iowa State, and the eighth and eleventh were at the University of Missouri–Columbia and Colorado State University, respectively. All symposia have been followed by proceedings edited by faculty of the host institution. Because final publication usually takes place elsewhere, papers here are brief, and often cover research in progress. ContentSequential Utilization of Mixed Sugars by Clostridium acetobutylicum, B. Hong, N. H. Choi, and L. T. Fan, Kansas State University The Effects of Dilution Rate on the Kinetics. of Anaerobic Acidogenesis, C. J. Huang, Colorado State University Ethanol Production by Zymomonas mobilis in Anaerobic Glucose-Limited Culture: A Yield Study, Mehmet D. Oner, Kansas State University Hydrolysis of Cellulosics by Enterobacteria, Michael R. Sierks, Iowa State University The Cellulase System of Chaetomium cellulolyticum, Nikhil Mehta, Colorado State University DNA Measurement as a Tool for Estimating Biomass Concentration in the Presence of Interfering Solids, Bamidele 0. Solomon, Kansas State University The Effect of Cellulose Crystallinity on Enzymatic Hydrolysis, Maria S. Bertran, Colorado State University High Performance Liquid Chromatography of Di- and Trisaccharides, Michael M. Meagher, Iowa State University Dynamics of Bubble Size .Distributions in Air-Lift Fermentors, c. H. Lee and Snehal A. Patel, Kansas State University A Thermal Coagulation Study of Alfalfa Leaf Proteins by Differential Scanning Calorimeter, Khalif Ahmed and Bruce Dale, Colorado State University Thermodynamic Efficiency of Photoautotrophic Growth, Hyeon Y. Lee, Kansas State University

Proceedings of the 14th Annual Biochemical Engineering Symposium

The Annual Biochemical Engineering Symposium series is devoted to presentations by students on their research topics. The fourteenth event, held in 1984, was organized at the University of Missouri–Columbia. It was attended by the biochemical engineering faculty and the students from Colorado State University, Iowa State University, Kansas State University, University of Missouri–Columbia, University of Missouri–Rolla and Washington University, St. Louis. Contents"Estimation of Product Formation Kinetics and Microbial Yield Parameters for Anaerobic Organic Acid and Solvent Production," M.D. Oner, Kansas State University "Characterization of Soy Protein Texturization in a Complex Bioreactor," J.L. Ibave, Colorado State University "Acid and Solvent Fermentations Using Mixed Cultures," D. Stevens, University of Missouri–Columbia "Preliminary Process Design for Ethanol from Sweet Sorghum Ensilage Feedstock," Keith D. Lange, Colorado State University "Lamella Settlers in Ethanol Fermentation," Yong Jayanata, University of Missouri–Columbia "Bubble Size Distribution in the Down Flow Section of an Air-Lift Column," Snehal A. Patel and C.H. Lee, Kansas State University "The Sensitivity of Plant Cells to Shear Stress," Morris Z. Resenberg and Eric H. Dunlap, Washington University, St. Louis "Estimation of Growth Yield Parameters Associated with Microbial Growth," Hyeon Y. Lee, Kansas State University "Capillary Gas Chromatography of Trimethylsilylated Trisaccharides," Etienne J.M. Selosse, Iowa State University "Subsite Mapping of an Endo-Xylanase Labeled Xylooligo-saccharides," Bernard Y. Tao, Iowa State University "Cellulase Enzyme Recycle," Kate M.V. Baptie, Colorado State University "Non-Homogeneous Poisson Renewal Reward Process for Modelling Enzymatic Hydrolysis of Cellulose," M.M. Gharpuray and L.T. Fan, Kansas State University

Proceedings of the Fifteenth Annual biochemical Engineering Symposium

This work represents the proceedings of the fifteenth symposium which convened at Colorado State University on May 24, 1985. The two day meeting was scheduled one month later than usual, i.e., after the spring semester, so that travelers from the Midwest (Iowa State University, Kansas State University and University of Missouri) could enjoy the unique mountain setting provided at Pingree Park. The background of the photograph on the cover depicts the beauty of the area. ContentsGreg Sinton and S.M. Leo, KSU. Models for the Biodegration of 2.4-D and Related Xenobiotic Compounds. V. Bringi, CSU. Intrinsic Kinetics from a Novel Immobilized Cell CSTR. Steve Birdsell, CU. Novel Microbial Separation Techniques. Mark Smith, MU. Kinetic Characterization of Growth of E. coli on Glucose. Michael M. Meagher, ISU. Kinetic Parameters of Di- and Trisaccharaide Hydrolysis by Glucoamylase II. G.T. Jones and A.K. Ghosh Hajra, KSU. Modeling and Simulation of Legume Modules with Reactive Cores and Inert Shells. S.A. Patel and C.H. Lee, KSU. Energetic Analysis and Liquid Circulation in an Airlift Fermenter. Rod R. Fisher, ISU. The Effects of Mixing during Acid Addition of Fractionally Precipitated Protein. Mark M. Paige, CSU. Fed-batch Fermentations of Clostridium acetobutylicum. Michael K. Dowd, ISU. A Nonequilibirium Thermodynamic Description of the Variation of Contractile Velocity and Energy Use in Muscle. David D. Drury, CSU. Analysis of Hollow Fiber Bioreactor Performance for MAmmalian Cells by On-Line MMR. H.Y. Lee, KSU. Process Analysis of Photosynthetic Continuous Culture Systems. C.J. Wang, MU. Kinetic Consideration in Fermentation of Cheese Whey to Ethanol.