1 resultado para Type II Site-Specific
em Bucknell University Digital Commons - Pensilvania - USA
Filtro por publicador
- ABACUS. Repositorio de Producción Científica - Universidad Europea (1)
- Abertay Research Collections - Abertay University’s repository (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (3)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (1)
- Aquatic Commons (3)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (6)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (2)
- Aston University Research Archive (15)
- Avian Conservation and Ecology - Eletronic Cientific Hournal - Écologie et conservation des oiseaux: (1)
- B-Digital - Universidade Fernando Pessoa - Portugal (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (18)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (14)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (3)
- Bioline International (1)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (74)
- Brock University, Canada (1)
- Bucknell University Digital Commons - Pensilvania - USA (1)
- CaltechTHESIS (7)
- Cambridge University Engineering Department Publications Database (5)
- CentAUR: Central Archive University of Reading - UK (25)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (35)
- Cochin University of Science & Technology (CUSAT), India (1)
- Coffee Science - Universidade Federal de Lavras (1)
- Collection Of Biostatistics Research Archive (1)
- CORA - Cork Open Research Archive - University College Cork - Ireland (3)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (1)
- Dalarna University College Electronic Archive (1)
- Deakin Research Online - Australia (28)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (4)
- Digital Commons at Florida International University (2)
- DigitalCommons@The Texas Medical Center (24)
- DRUM (Digital Repository at the University of Maryland) (1)
- Duke University (6)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (7)
- Glasgow Theses Service (1)
- Helda - Digital Repository of University of Helsinki (12)
- Illinois Digital Environment for Access to Learning and Scholarship Repository (2)
- Indian Institute of Science - Bangalore - Índia (79)
- Instituto Politécnico do Porto, Portugal (1)
- Lume - Repositório Digital da Universidade Federal do Rio Grande do Sul (1)
- National Center for Biotechnology Information - NCBI (127)
- Nottingham eTheses (1)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (6)
- Publishing Network for Geoscientific & Environmental Data (22)
- QSpace: Queen's University - Canada (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (75)
- Queensland University of Technology - ePrints Archive (126)
- Repositorio Academico Digital UANL (1)
- Repositório Científico da Escola Superior de Enfermagem de Coimbra (1)
- Repositório Científico da Universidade de Évora - Portugal (2)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (1)
- Repositório Institucional da Universidade de Aveiro - Portugal (3)
- Repositório Institucional da Universidade de Brasília (1)
- Repositório Institucional da Universidade Estadual de São Paulo - UNESP (2)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (58)
- Repositorio Institucional Universidad de Medellín (1)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (1)
- SAPIENTIA - Universidade do Algarve - Portugal (1)
- Savoirs UdeS : plateforme de diffusion de la production intellectuelle de l’Université de Sherbrooke - Canada (2)
- Universidad del Rosario, Colombia (2)
- Universidad Politécnica de Madrid (12)
- Universidade Complutense de Madrid (2)
- Universidade de Lisboa - Repositório Aberto (1)
- Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP) (2)
- Universidade Federal de Uberlândia (1)
- Universidade Federal do Rio Grande do Norte (UFRN) (3)
- Universita di Parma (2)
- Université de Lausanne, Switzerland (5)
- Université de Montréal (7)
- Université de Montréal, Canada (28)
- University of Canberra Research Repository - Australia (2)
- University of Connecticut - USA (1)
- University of Michigan (19)
- University of Queensland eSpace - Australia (22)
- University of Washington (2)
- WestminsterResearch - UK (1)
Resumo:
Honeybees are an essential component of today¿s agricultural system because of their role as pollinators. However, viruses, including a member of the Picornavirales order known commonly as Deformed Wing Virus (DWV), are compromising the health of honeybee colonies. Many picornaviruses, such as poliovirus, have been studied in depth because of their relation to human disease, but also because of their use of an Internal Ribosome Entry Site (IRES) to initiate translation. The primary goal of this thesis was to determine if the 5¿ Non-Translated Region (NTR) of Deformed Wing Virus (DWV) functions as an IRES. A secondary goal was to determine if there are specific parts of that 5¿ NTR that are important to IRES function. Six plasmids were constructed by inserting three different sections of the 5¿ NTR of DWV, in both sense and antisense directions, between two reporter genes. These plasmids, along with several control plasmids, were transfected into Sf9 cells, and post-transfection luciferase assays were conducted. Results were inconclusive. This could have been due to an inability of the plasmids to be expressed in Sf9 cells, an error in the construction of the plasmids, or a mechanical error in the assay procedure. At this time it appears most likely that the 5¿ NTR of DWV may be cell-type or species specific, and the next step would be to transfect the plasmids into a recently developed cultured honeybee cell line.