Nitrate reductase activity in leaves and stems of tanner grass (Brachiaria radicans Napper)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Chemical treatment of post-harvest Marandu grass seed residues with different moisture contents

Objetivou-se avaliar o efeito do tratamento químico com ureia (3 ou 5% na MS) e amônia anidra (3% na MS) no feno de resíduo pós-colheita de sementes de Brachiaria brizantha, cv. Marandu, contendo diferentes teores de umidade (15, 25 ou 30%). O delineamento experimental foi em blocos ao acaso com oito tratamentos e quatro repetições (camadas de fardos dentro das pilhas). O feno tratado com 3% de amônia anidra com 15% de umidade ocasionou redução de 84,3 para 79,1% nos teores de fibra em detergente neutro (FDN) e elevação de 37,3 para 55,5% na digestibilidade in vitro da matéria seca (MS) em relação ao grupo controle. A variação na umidade não alterou de maneira significativa a ação da amônia, cujos valores médios foram 77,6% de FDN e 57,3% de digestibilidade in vitro da MS. O feno com 5% de ureia reduziu os teores de FDN de 84,3 para 79,6% em relação ao feno não-tratado, o que tornou necessário o aumento da umidade para 30% para maior efeito sobre a digestibilidade da MS, que aumentou 12 unidades percentuais.

Nutritional evaluation of elephant-grass silages with byproduct of annato

Objetivou-se avaliar o valor nutritivo de silagens de capim-elefante contendo subproduto do processamento do urucum. Vinte ovinos machos, não-castrados, foram distribuídos em delineamento inteiramente casualizado com cinco dietas (0; 4; 8; 12 e 16% de subproduto de urucum na silagem) e quatro repetições, e utilizados para estimar o consumo, a digestibilidade aparente de nutrientes, o teor de nutrientes digestíveis totais e o balanço de nitrogênio. A adição de subproduto de urucum elevou os consumos de matéria seca (MS), matéria orgânica (MO), proteína bruta (PB), fibra em detergente neutro (FDN), fibra em detergente ácido (FDA), extrato etéreo (EE), carboidratos não-fibrosos (CNF), carboidratos totais e nutrientes digestíveis totais (NDT), tanto em relação ao peso vivo (%PV) quanto em relação ao peso metabólico (g kg-1PV0,75). As digestibilidades de MS (55,95%), MO (58,19%), PB (45,34%), FDN (52,79%), FDA (45,79%), EE (34,96%) e CNF (99,86%) não foram influenciadas pela adição de subproduto de urucum. A inclusão de subproduto de urucum na silagem de capim-elefante promoveu aumento na digestibilidade dos carboidratos totais, no teor de NDT e no balanço de nitrogênio. A adição de subproduto de urucum em níveis de até 16% da matéria natural na ensilagem de capim-elefante melhora o consumo de nutrientes e o balanço de nitrogênio.

Morphogenetic and tillering dynamics in Tanzania grass fertilized and non-fertilized with nitrogen according to season

The objectives of this study were to evaluate morphogenetic characteristics and tillering dynamics in Tanzania grass fertilized and non-fertilized with nitrogen, under intermittent grazing, in the spring and the summer. The main plots were composed of four nitrogen rates (0, 100, 200 and 300 kg/ha) and the subplots were growth seasons: spring (October, November and December) and summer (January, February and March). The experimental design was of randomized block with plots subdivided by time (seasons of the year) and four replications. Urea was used as nitrogen supply and was divided into two applications: one in the spring and another in the summer. The experimental units fertilized with N rates of 200 and 300 kg/ha showed six cycles of pasture, with an average of 27 days of pasture interval, while the treatments with no fertilization and 100 kg/ha of N showed only four and five cycles of pasture, respectively. Leaf elongation rate (LER) and the leaf appearance rate (LAR) increased linearly with increasing of N rates. The greatest population density occurred in summer with the higher nitrogen rates. The treatment without N fertilization showed the lowest growth of tiller population, while the other treatments exhibited growth rates above 50% when compared with non-fertilized samples. Nitrogen rates significantly affect the leaf appearance rate and the leaf elongation rate, as well as the number of live leaves in plants of Tanzania grass in both spring and summer.

Tillering dynamics in Guinea grass pastures subjected to management strategies under rotational grazing

This study was carried out to analyze the tillering profile of Guinea grass (Panicum maximum cv. Tanzania) pastures subjected to two grazing frequencies (time necessary to intercept 90 and 95% of the incoming light) and two post-grazing heights (30 and 50 cm) in the period from November 2005 to October 2006. The experimental design was of completely randomized blocks with three replications, in a 2 × 2 factorial arrangement. At the end of the spring, pastures managed with 90% light interception showed greater tiller appearance rates in relation to pastures managed with 95%, regardless of post-grazing height. In the summer and fall, pastures managed with post-grazing height of 30 cm showed higher tiller appearance rates in comparison with pastures managed at 50 cm, regardless of grazing frequency. Concerning the tiller mortality rates, in the summer, higher values were found for pastures managed at 90/50 and 95/30 (interception/height), intermediate values at 90/30 and lower values in those managed at 95/50. Pastures managed at 90/30, 95/30 and 95/50 in the fall presented greater tiller mortality rates than those managed at 90/50. These differences do not occur in the winter/beginning of spring. The stability index remained above 1 all through the experimental period. All management strategies evaluated are adequate for Guinea grass.

Performance of Nellore young bulls on Marandu grass pasture with protein supplementation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Prediction of dry matter intake based on ruminal degradation from milking cows grazing coast-cross grass

Dry matter intake (DMI) of coast-cross grazing by crossbred Holstein-Zebu and Zebu lactating cows was calculated using in vitro dry matter digestibility from extrusa (four esophageal fistulated cows) and fecal output estimate with mordent chromium. Pasture was rotationally grazed with three days grazing period and 27 days testing period, adopting a stocking rate of 1.6 and 3.2 cows/ha, during the dry and rainy season respectively. Voluntary DMI was estimated from degradation characteristics using different equations. Predicted coast-cross DMI varied with models. The prediction of tropical forages dry matter intake from equations based in ruminal degradation parameters needs farther investigation before being employed in practice.

Structural organization in leaf blade and sheath of elephant-grass cultivars (Pennisetum purpureum Schum. - POACEAE).

Elephant-grass is known as a great feed to dairy and beef cattle at tropical regions. Its agricultural aspects have been very observed. However, its botanical, mainly anatomical characteristics, are few studied. The objective of this work was to investigate the anatomical changes occurred in leaves (leaf blades and sheaths) of three elephant-grass cultivars (Pennisetum purpureum Schum. cultivar Roxo, EMPASC 307 Testo and EMPASC 309 Areia) at three stages of physiological maturity (4, 8 and 16 weeks after sprouting). In general, the three cultivars presented similar anatomy. A unique feature, the presence of aerenchyma was found in the leaf sheath of all three cultivars, at the second harvest (a weeks).

Mineralogy and forms of potassium in red yellow latosol of a guava (Psidium guajava) tree orchard

A trial was conducted during 1994-95 to study the effect of potassium fertilization on a guava (Psidium guajava L.) culture for 3 years. The control plots (without K) showed fruit production and potassium exportation that did not agree with the levels obtained by chemical analysis of the soil. Physical, chemical, mineralogic and morphologic analyses were performed on the red yellow latosol to identify minerals able to supply potassium, with emphasis on the fact that guava trees have a considerably widespread root system. The results obtained confirmed the presence of minerals in this soil that can supply potassium to the trees through weathering. Feldspars were identified in the silt fraction and micas in the clay fraction by X-ray diffractometry. The determination of total potassium revealed that the silt fraction of the soil had the largest absolute amounts of potassium, followed by clay. However, in view of its greater content, clay was the fraction that contributed most to the total amounts of potassium detected.

Chemical Composition and fermentative characteristics of Elephant grass (Pennisetum purpureum Schum.) silages added of increasing levels of mango (Mangifera indica L.) byproducts

Aiming at evaluating the use of those byproducts on ruminant feeding an experiment has been carried out in the Forage Research department (www.npf.ufc.br), in order to evaluate the effects of addition of increasing levels of mango processing byproducts (MB) in Elephant grass silages. Twenty experimental silos made of PVC pipe (100 x 340 mm), in a completely randomized design with 5 levels of addition (0; 5; 10; 15; and 20%) of MB and 4 replications. Upon 32 days of ensilage, samples were collected for determination of levels of dry Matter (DM), crude protein (CP), ethereal extract (EE), neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicelluloses (HC), ammonia nitrogen (N-NH3), and pH values. Data were evaluated by analyses of variance and regression analyses using the F test at the 5% level. The levels of DM and CP increased of 0.50 and 0.03 percentage units, respectively, for every 1% of MB added. The levels of NDF and ADF decreased of 0.51 and 0.24 percentage units, respectively, for every 1% of MB added. The levels of N-NH3 decreased from 4 to 23 percentage units while pH values remained constant (p>0.05), with average values of 3.29. Addition of 20% of MB improved silages chemical composition by promoting an increase in the levels of MS and reduction in the levels of fiber.

In situ degradability of hand harvested or extrusa samples of Tanzania grass (Panicum maximum, Jacq.)

In order to compare the in situ degradability of tanzania grass samples obtained as by extrusa or hand plucked, three ruminal fistulated cows were used in a completely randomized block design with split-plot scheme. Five grams of extrusa or hand harvested grasses were placed in nylon bags rumen incubated during 3, 6, 12, 24, 48, 96 and 120 hours. The degradability of DM, CP, NDF and ADF were, in this sequence, 62.59, 80.88, 50.73 and 46.65%, for hand-harvested grass; and 79.53, 90.97, 71.21 and 65.68%, for extrusa. In situ degradability data of hand harvested samples were not reliable.

Floral development and embryology in the early-divergent grass Pharus

A detailed study of floral ontogeny, anatomy, and embryology in two (of six) species of Pharus is presented as part of a series of comparative investigations on early-divergent grasses. Pharus is a taxonomically isolated genus belonging to the earliest-diverging grass lineage with a true grass spikelet. It is unusual in possessing remarkably dimorphic florets: male florets possess two lodicules, six stamens, and a pistillode, whereas female florets lack lodicules entirely but possess six staminodes and a tricarpellary ovary with three stigmas. The rudimentary lodicules in male florets are initiated after the stamen whorls. There are most commonly six androecial organs, but in some florets, a five-staminate condition was observed, resulting from suppression of the abaxial stamen from the inner whorl, or even a four-staminate condition resulting from subsequent fusion of the two adaxial outer stamens (i.e., elements of both whorls). Thus, the pattern of floral zygomorphy in Pharus differs from that of many other grasses. Centrifixed anther attachment is reported for the first time in Pharus, resembling the condition in another early-divergent grass, Anomochloa, though anthers are introrse in Anomochloa compared with latrorse in Pharus. Anther wall development is of the reduced type in Pharus, in contrast to most other monocots. Microsporogenesis is of the successive type, as in many other monocots. The ovary develops from three distinct primordia and is unilocular with a single ovule and a pronounced ovary beak that is highly characteristic of Pharus. There is a hollow style, in contrast to the solid styles that are common in many other grasses. The embryo is highly differentiated, as in other grasses, with a distinct epiblast and a small cleft between the scutellum and the coleorhiza.

Effect of ammoniation and moisture-content on the quality of signal grass (Brachiaria-decumbens stapf) hay

The experiment was carried out to evaluate the effects of two moisture levels (18-20% and 13-15%) and three anhydrous ammonia levels (0.0; 1.5; 3.0% of NH3) on the quality of Brachiaria decumbens Stapf hay. The hay was bailed in April and weighed and treated under plastic cover during 30 days. The hay presented a similar chemical composition when bailed with high or low moisture. The percentages of NDF (80.59; 77.61; 76.10%); hemicellulose (32.56; 29.48; 28.76%) and lignin (9.53; 8.21; 7.54% decreased and the percentages of crude protein (4.04; 11.35; 13.22%) and IVDMD (36.78; 49.72; 54.33%) increased as the NH3 level increased. The fractions ADF, cellulose, and ADIN did not change due to the ammoniation. The incidence of fungi decreased with application of NH3 being the better results obtained with the 1.5% treatments.