301 resultados para Tecido adiposo Teses
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Fundao de Amparo Pesquisa do Estado de So Paulo (FAPESP)
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The objective of this study was to evaluate the culture of equine bone marrow mononuclear fraction and adipose tissue - derived stromal vascular fraction cells in two different cell culture media. Five adult horses were submitted to bone marrow aspiration from the sternum, and then from the adipose tissue of the gluteal region near the base of the tail. Mononuclear fraction and stromal vascular fraction were isolated from the samples and cultivated in DMEM medium supplemented with 10% fetal bovine serum or in AIM-V medium. The cultures were observed once a week with an inverted microscope, to perform a qualitative analysis of the morphology of the cells as well as the general appearance of the cell culture. Colony-forming units (CFU) were counted on days 5, 15 and 25 of cell culture. During the first week of culture, differences were observed between the samples from the same source maintained in different culture media. The number of colonies was significantly higher in samples of bone marrow in relation to samples of adipose tissue.
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Coordenao de Aperfeioamento de Pessoal de Nvel Superior (CAPES)
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Ps-graduao em Fisiopatologia em Clnica Mdica - FMB
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Fundao de Amparo Pesquisa do Estado de So Paulo (FAPESP)
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Fundao de Amparo Pesquisa do Estado de So Paulo (FAPESP)
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Fundao de Amparo Pesquisa do Estado de So Paulo (FAPESP)
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Fundao de Amparo Pesquisa do Estado de So Paulo (FAPESP)
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Brazil has the fourth largest horse herd in the world, this is due the recognition and appreciation that the different equestrian games are having within the country. Injuries of the tendon, especially in the digital flexor tendon, are the main cause of athletic life reduction among horses. The treatment of tendinitis in horses seeks full recovery of the damage tissue reestablishing the function previously lost, however conventional treatments have proven to be ineffective when considered the quality of the scar tissue and the rate of recurrence. Due to this, the use of adult stem cells to the treatment of musculoskeletal injuries of horses has been studied for some time. This method of treatment consists of aspiration of bone marrow or removal of subcutaneous fat tissue and implantation of these cells in the injured tissue. After obtaining the bone marrow the implantation can be performed with total bone marrow, with the mononuclear fraction of MSC or with cells cultured in vitro. From the fat tissue is used the stromal vascular fraction obtained by collagenase digestion, followed or not by cell culture. According to some studies, cell therapy with material obtained from bone marrow or adipose tissue has shown to be viable, given that these materials are abundant in repair components such as mesenchymal stem cells (MSC), growth factors and other components of the collagen matrix. Several studies using both types of cells have shown great potential and promising clinical results. However, knowledge of the biology and characterization of these cells remain largely unknown, and therefore is needed great care and caution when using stem cells for the treatment of musculoskeletal disorders in horses
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A insuficincia renal aguda (IRA) uma patologia que apresenta alta incidncia na populao e elevada morbimortalidade. Apesar de todos os avanos teraputicos j obtidos, essas taxas ainda continuam elevadas. Uma possvel alternativa, atualmente sugerida, seria o transplante de clulas-tronco. O processo regenerativo das clulas-tronco mesenquimais (CTMs) j foi demonstrado em diversos modelos experimentais e em alguns ensaios clnicos. O mecanismo de ao mais sugerido a ao parcrina das CTMs na rea lesada. Ainda, sabe-se que nesse ambiente, citocinas pr-inflamatrias, como TNF- e IFN, ativam as CTMs para seu papel reparador. O presente estudo busca analisar o papel do IFN na ativao das CTMs em modelos renais. As CTMs de animais nocautes para receptor de IFN (IFNR KO) e de animais selvagens (controle/ C57/Bl6) foram isoladas do tecido adiposo. Essas clulas foram caracterizadas por imunofenotipagem e diferenciao em adipcitos e ostecitos. A leso renal aguda foi obtida atravs do clampeamento dos pedculos renais de camundongos machos C57/Bl6, por 45 min. Aps 4hs da leso isqumica, as CTMs IFNR KO e CTMs controles foram administradas intraperitonealmente, e 24hs aps a cirurgia os animais foram sacrificados. O tratamento com CTMs selvagens apresentou significativa reduo dos nveis de uria e creatinina srica. No entanto, a reduo desses nveis sricos com CTMs IFNR KO foi menos intensa. Com relao anlise da resposta inflamatria do rim, os dados demonstram que a expresso de RNAm de IL-6 maior nos animais tratados com CTMs IFNR KO quando comparada ao tratamento com CTMs selvagens; porm, os dois tratamentos apresentam expresso reduzida em comparao aos animais no tratados. J a expresso de RNAm de IL-10 maior em animais tratados com CTMs em comparao aos no tratados... (Resumo completo, clicar acesso eletrnico abaixo)
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A sndrome metablica um mal que, segundo projees, poder no ano de 2010, acometer de 50 a 75 milhes de pessoas somente nos Estados Unidos. Dessa forma, de grande interesse a implementao de procedimentos mais eficazes para preveno e tratamento desta doena. Uma vez que existem limitaes nas pesquisas com seres humanos, torna-se necessrio o desenvolvimento de modelos experimentais apropriados ao estudo desta questo. Na busca de um modelo experimental adequado ao estudo do papel do exerccio na preveno e no tratamento da sndrome metablica, o presente estudo analisou o perfil metablico e a capacidade aerbia de ratos mantidos com dieta rica em frutose, substrato que tem sido associado sndrome metablica, na vida intra-uterina e ps-natal. Foram utilizadas ratas adultas (90 dias) da linhagem Wistar, alimentadas durante a prenhez e a lactao com dois tipos de dieta: balanceada (AIN-93G) e rica em frutose (60% de frutose). Durante a amamentao, os filhotes foram distribudos em ninhadas pequenas (4/me) ou adequadas (8/me). Aps o desmame, manteve-se as mesmas dietas at os 90 dias, quando foram analisados: tolerncia glicose (teste de tolerncia glicose), sensibilidade perifrica insulina (teste de tolerncia insulina); capacidade aerbia (determinao da mxima fase estvel de lactato em natao), contedo pancretico de insulina; peso e concentrao de lipdios totais no tecido adiposo de diferentes regies e concentraes sricas de glicose, insulina, triglicerdeos, colesterol total, colesterol LDL, colesterol HDL. Constatou-se que a dieta rica em frutose conduziu os animais a resistncia insulina. O grupo FP apresentou dislipidemia, com aumento nas concentraes sricas de colesterol total e triglicerdeos, mostrando que o modelo animal avaliado potencialmente interessante para o estudo da sndrome metablica.
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Mesenchymal stem cells (MSCs) are adult multipotent cells with fibroblastoid morphology and adherent to plastic. Furthermore, they can be obtained from different sources. Besides bone marrow, these cells are taken from umbilical cord blood, umbilical vein, saphenous vein, peripheral blood, arteries, liver and fetal pancreas, placenta, dental pulp and adipose tissue. MSCs derived from adipose tissue are important because of the abundant number of cells that can be obtained from this tissue, easy access and little discomfort to the patient. This study compared two techniques for obtaining MSCs from adipose tissue: mechanical dissociation (MD) and enzymatic digestion (ED). We also analyzed the inter-species cross-reactions using commercial monoclonal antibodies directed against surface antigens of stem cells from different species: mouse, horse, rabbit, monkey and human. We found that MD technique is favorable in relation to ED within 15 days of culture, and ED is more efficient in the first days of culture. The data also showed that MD causes less damage to cellular DNA. About inter-species cross-reactions, the monoclonal antibody A69 directed against stem cells from rabbits, which can be used in veterinary medicine, particularly in research involving horses
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The cancer anorexia-cachexia syndrome is the most common paraneoplastic syndrome in Veterinary Medicine. It is characterized by severe loss of muscle mass and adipose tissue resulting in severe unintentional weight loss, anemia, fatigue, negative nitrogen balance, immune dysfuntion and other metabolic disturbances. The SAC is not only a result of inadequate intake of nutrients. The tumor requires large amounts of nutrients to allow growth and causes changes in pacient metabolism to get this energy. Recent studies suggest that the metabolic changes by cancer can be measured by hormones and cytokines produced or by the patient or the tumor, but this not completely understood. Animals with SAC have lower survival time, the greater chance of complications during treatment and lower quality of life. With the increase in the number of cancer cases in domestic animals and longer lifespan after diagnosis of malignant disease through the use of antineoplastics drugs, diagnosis and treatment of cancer anorexia-cachexia syndrome has shown great importance in that patients may have higher survival then better quality of life. This paper aims to provide information about this complex and multifunctional syndrome and its possible treatments
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Muscular dystrophy refers to a group of more than 30 genetical disorders characterized by progressive weakness and degeneration of the skeletal muscle. No effective therapy is available at present. Recent studies have reported that the transplantation of stem cells can offer an important potential therapy for genetic diseases. Adult bone marrow mesenchymal stem cells have been identified as a nonhematopoietic stem cell population capable of self-renewal with the ability to differentiate into many cell lineages, including bone, fat, cartilage and connective tissue. Because of their similarity with muscle progenitor cells, when they are injected in affected individuals, they are able to migrate into areas of skeletal muscle degeneration and participate in the regeneration process. The adipose tissue represents an alternative source of MSCs that, as the MSCs derived from bone marrow, are capable of in vitro differentiation into osteogenic, adipogenic, myogenic and chondrogenic lineages. The objective of this project is to investigate the in vitro myogenic potential of mesenchymal stem cells derived from murine bone marrow and adipose tissue. Four experimental groups were analyzed: mice from lineages Lama2dy-2J/J and C57black and, C2C12 lineage cells and transformed C2C12 expressing the eGFP protein. MSCs cultures were obtained by flushing the bone marrow femurs and tibials with -MEM or by the subcutaneous and inguinal fat from the mice. Their characterization was done by flow cytometry and in vitro differentiation. Muscle differentiation was studied through the analysis of the expression of transcriptional factors involved in muscle differentiation and/or the presence and amount of specific proteins from muscle differentiated cell. The pluripotency from bone marrow MSCs of the two lineages was evidenced and, in the muscular differentiation... (Complete abstract click electronic access below)
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Diante da constatao clnica de que muitos pacientes no conseguem benefcio completo da remisso da ulcerao em membros inferiores com os tratamentos convencionais, o Setor de Biotecnologia do Hemocentro de Botucatu estabeleceu o protocolo de produo de curativos bioativos, que interagem com a leso e estimulam via hormnios de crescimento (PDGF, VEFG, FGF e outros) a recuperao da rea danificada por induo das clulas-tronco comissionadas da pele. Estudos realizados na Faculdade de Qumica de Araraquara culminaram com a purificao do princpio ativo do fitoterpico Cordia ecalyculata para analisar in vitro o seu alto potencial antiinflamatrio. Seus diferentes extratos foram enviados ao Laboratrio de Engenharia Celular da Faculdade de Medicina de Botucatu: extrato bruto (EB), fase aquosa (FA), fase hexnica (FH) e fase etrea (FE), que foram testados em diferentes concentraes em cultura de clulas-tronco mesenquimais humanas (CTM), oriundas de tecido adiposo, com a finalidade de analisar, atravs de caspase-3, marcador intracelular, o ndice de apoptose. Aquele que apresentar o melhor resultado ser adicionado frmula dos biocurativos. Este trabalho tem os objetivos de avaliar a contribuio da cultura celular de CTM nos testes de tolerncia com Cordia ecalyculata, estabelecer o ndice de apoptose celular e avaliar o tempo de confluncia maior que 80% na cultura de CTM em diferentes concentraes de C. ecalyculata. Os resultados encontrados comprovaram que os extratos que apresentaram os melhores desempenhos foram os da FA em todas as concentraes e FE de 25g/mL. Os extratos FE nas demais concentraes demonstraram um desempenho insatisfatrio,o que nos indica que uma concentrao entre 25 e 50g/mL seja o limite entre a boa execuo nas clulas e toxicidade. Mais testes tero que ser realizados para demonstrar qual essa concentrao de FA realmente melhor a ser utilizada
