Avaliação das alterações celulares nos ovócitos de fêmeas semi-ingurgitadas de carrapatos Rhipicephalus sanguineus (Acari: Ixodidae) decorrentes da exposição ao óleo de andiroba

Os carrapatos são considerados um dos mais importantes grupos dentre os artrópodes, por serem vetores de agentes patogênicos, que podem atacar animais, bem como o próprio homem. Dentre as diversas espécies de carrapato destaca-se a do Rhipicephalus sanguineus, pertencente à família Ixodidae e com ampla distribuição em todos os continentes. Atualmente esta espécie é considerada uma praga urbana, de grande importância médico-veterinária, que parasita principalmente o cão doméstico. Os ovários de carrapatos são considerados órgãos vitais para o sucesso biológico deste grupo de animais. Neste sentido, desenvolver pesquisas para se conhecer a ação de produtos naturais com reconhecida ação repelente ou acaricida, sobre o sistema reprodutor feminino de carrapatos, traria importantes informações para um melhor entendimento dos efeitos da ação destes produtos nos ovócitos desses ectoparasitas, contribuindo principalmente para o desenvolvimento de métodos de controle alternativos menos tóxicos para os organismos não-alvo e menos poluentes para o meio ambiente. Dessa forma, o presente estudo analisou as alterações morfofisiológicas causadas por diferentes concentrações do óleo de andiroba no ovário de fêmeas semiingurgitadas de carrapatos R. sanguineus

Análise da frequência de micronúcleos frente á utilização de colutório bucal com base alcoólica em indivíduos fumantes e não fumantes

Micronuclei (MN) originate from chromosome fragments or whole chromosomes that lag behind at anaphase during cell division can occur due to excessive exposure to harmful agents, defects in mitosis and / or DNA repair process. The study evaluated the frequency of micronuclei in cytological samples after daily use of alcohol -based mouthwash mouth in smokers and non-smokers. We assessed 30 subjects, aged between 17-42 years and established as exclusion criteria: alcohol consumption; periodontal disease; systemic diseases that may interfere with the integrity of the oral mucosa; individuals with medical or pharmacological history that affect the performance of the study, family history of malignancies. For smokers the inclusion criterion was a minimum consumption of 20 cigarettes / day, and the contrast required for non-smokers. The sample was divided into 02 experimental groups: Group 1 Smokers and Group 2 Non-Smokers, both groups performed mouthwash (alcohol 21,6%) with Listerine® Defense of Teeth and Gums for 04 weeks cytological collection were performed on buccal mucosa and portion median border of the tongue, one for initial, week 0 control, and subsequent three weeks with continued use of mouthwash. The collected material was stained by specific staining Feulgen / Fast Green. The analysis of 3000 cells / individual was performed by light microscopy statistics used Kruskal- Wallis and Mann-Whitney tests for frequency MN. The frequency of micronuclei in buccal mucosa was 1.6 ± 1.75 and 1.46 ± 2.06 for the site language to Group 1 Smokers of showing a slight increase compared with Group 2 Non-smoking, which showed a variation of 1.2 ± 1.53 for the buccal mucosa site and 0.86 ± 1.41 for the site language. However, this difference was not statistically significant. The results suggest that alcohol-based mouthwash tested is not an inducer of genotoxic changes, thus not changing the frequency...

Avaliação de recursos hídricos sob a influência de atividades de uma indústria do petróleo, por meio de análises ecotoxicológicas integradas a parâmetros ambientais

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Perfil de fatores de virulência das Escherichia coli isoladas do útero de vacas holandesas após o parto e sua relação com a microbiota uterina

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo da migração de células peritoneais na paracoccidioidomicose experimental murina: avalaição do efeito da estimulaçao de células peritoneais por lectina sobra a evolução da lesão paracoccidióidica

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Epitheliotropic cutaneous T-cell lymphoma associated with melanoma in a dog: case report

Several types of tumors affect dogs' skin. Simultaneously occurring neoplasms with different histological patterns might be rarely present in the same animal. This paper describes the occurrence of epitheliotropic cutaneous T-cell lymphoma and melanoma in a dog. The animal had nodular lesions in the abdominal region and serpiginous plaques on the dorsal region of the trunk. Cytology evidenced malignant fusiform cells from the abdominal lesions as well as few round cells from the dorsal. The histopathological examination of the abdominal lesions showed dermis with polygonal to spindle-shaped neoplastic cells. The lesion of the dorsal region evidenced neoplastic round cells with generally distinct cell borders and a moderate amount of eosinophilic cytoplasm. Abdominal lesions were positive for Melan A. Dorsal and forelimb lesions were positive for CD3. This study reports the occurrence of epitheliotropic cutaneous T-cell lymphoma and malignant melanoma in a crossbred Boxer dog and discusses the importance of performing immunohistochemical profile to confirm the phenotype of the tumor.

Investigação do papel de HJURP (Holliday Junction Recognizing Protein) na resistência de células de glioblastoma multiforme à radiação ionizante

Pós-graduação em Biociências e Biotecnologia Aplicadas à Farmácia - FCFAR

Transplante interespecífico de espermatogônias-tronco de Brycon orbignyanus em Astyanax altiparanae (Teleostei: Characidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação da maturidade pulmonar de cordeiros nascidos a termo e prematuros pela análise citológica, Test de Clements e contagem de corpos lamelares do líquido amniótico

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Maturidade pulmonar e fetal de bezerros da raça holandesa preta e branca tecém-nascidos, a termo e hígidos: análise do líquido amniótico

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Detecção de co-infecções por Leishmania (l.) chagasi, Trypanosoma evansi, Toxoplasma gondii e Neospora caninum em cães

Co-infections by Leishmania (L.) chagasi, Trypanosoma evansi, Toxoplasma gondii and Neospora caninum in dogs were investigated. Amastigotes forms of Leishmania spp. were detected by cytopathological analysis of lymph nodes in 46,42% (39/84) of dogs. In a male dog, adult, without defined breed, from rural area and positive for Leishmania, were observed flagellated forms of T. evansi in blood smear. By immunofluorescence antibody test, 5,95% (5/84) of dogs were considered reactive to T. gondii, with titer equal to or higher than 1:64, while 3,57% (3/84) were reactive to N. caninum, with titer ≥1:50. Among the animals with visceral leishmaniasis, one showed positive serological response to T. gondii and two for N. caninum. All dogs reactive to N. caninum were from rural area and the predominance of infection by T. gondii was in dogs from urban area. A young male dog from the rural area and seropositive for T. gondii showed Ehrlichia spp. morulae in the cytology and positive reaction for canine distemper virus. Thus, further studies are needed to assess the epidemiology of these infections in canine population, especially with respect to the reservoirs of Trypanosoma spp. in rural areas.

Programa de diagnóstico e prevenção de câncer da boca: uma estratégia simples e eficaz

The program of diagnosis and prevention of oral cancer of Univag - University Center aims to increase preventive and early oral diseases with emphasis on oral cancer, aiming to reduce the morbidity and mortality through educative actions, spreading of the auto-examination, performed by dental students of UNIVAG since the second half of 2005 to the present day. The dental students of 1st, 2nd, 3rd and 4th semesters were trained using practice-theoretical classes, for realization the exams preventives and diagnostics, such as self-examination, exfoliative cytology and biopsy of oral mucosa, and even present educative actions. Analyzing the preliminary results were observed increase in the number of diagnoses and referral of diagnosed with oral cancer for treatment in referral services. It was concluded that the program of diagnosis and prevention of oral cancer can make possible early diagnosis and adoption of preventive measures, consequently improving survival rates and life quality in population.

Perfil epidemiológico dos participantes do Programa de diagnóstico e prevenção do câncer de boca em Mato Grosso

To determine the profile of the patients who participated in the program of oral cancer diagnosis and prevention in Mato Grosso, from 2008 to 2009. Materials and Methods: The medical records of the treated patients were examined. Results: 1.293 records were analyzed, with a predominance of female patients (60%) and a greater participation of patients from 10 to 19 years of age (20%). Reports of pre-existing illnesses amounted to 302 recorded cases. In addition, 292 variations of normality could be also identified. During the period, 25 participants were recommended for exfoliative cytology and 133 for biopsy, as they presented oral lesions that raised the suspicion of malignancy. Conclusions: Campaigns to raise awareness of and prevent oral cancer are very important and should be extended to the entire population of the state of Mato Grosso, either by means of the local University Center (UNIVAG), or by other agencies and health institutions.

Carcinoma de lábio com prognóstico sombrio devido conduta médica inadequada

The Squamous Cell Carcinoma is what more happens among the oral cancers, being considered sweats incidence in more than 90%. As important prognostic factor competes the early diagnosis and the immediate institution of the treatment, that it can involve radiotherapy and chemotherapy besides the surgery. The initial lesions don’t offer great diagnostic difficulties and now complemental laboratory exams define the diagnosis quickly, In the present report, the initial medical conduct was inadequate, with incomplete interpretation of the laboratorial result and adoption of inadequate therapeutic conduct, what resulted in constant evolution of the lesion. The patient came to the consultation with that medical history, after six months of the first attendance accomplished in another center. To the physical exam, infiltrative ulcerated lesion was observed in the lower lip, with having accentuated tissue loss superficially. The patient accused spontaneous pain and she did use an ointment, recommended six months ago for occasion of the first attendance. As diagnostic conduct was indicated the accomplishment of cytology and biopsy, being defined the diagnosis of Squamous Cell Carcinoma. The patient was immediately guided to the medical service of its municipal district for manegement of the specialized oncologic treatment. The patient was submitted the surgery followed by radiotherapy, being verified involvement of another structures by contiguity. Now, the patient meets abed, without viable perspectives of cure. The present report emphasizes the importance of the clinical knowledge and professional responsibility in attendance of cases of that nature.

Uma descrição semiótica da metáfora no ensino de biologia: asserções sobre a célula animal

The main objective of this survey is describing signly the metaphor learning resource, with focus on biology education. Our teorical reference is the peircean semiotics, because it means solid heritage to code surveys implicated at science education, it led us to do an explanation about the main lines about that reference, addressed to researchers and lecturers interested in those reasonings. In the description yearned, another objectives appear: demonstrate diferences between metaphor/analogy and pragmatic analogy; to list metaphors, from cell representation analysis, and argue about principals diferences and probable cognitive consequences between the metaphor event in the visual representation and speech sentences. Therefore we analysed the animal cell of a high school first grade studying book – biology – cell biology – used by São Paulo and another state students. The findings demonstrate the undeniable importance of the metaphor as a learning tool in the biology education, and new findings about that, such as its limits in the concept elaboration, gnosiologic consequences for receptioning, the search needs for propositions– pictures between concept relations – in the science speech construction, and other results.