Proteins of Leishmania (Viannia) shawi confer protection associated with Th1 immune response and memory generation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Factor XIII Val34Leu is a genetic factor involved in the aetiology of venous thrombosis

A mutation in the factor XIII gene (FXIII Val34Leu) gene was recently reported to confer protection against myocardial infarction, but its relationship with venous thrombosis is unknown. In addition, a mutation in the 5'-untranslated region of the FXII gene (46 C→T) was identified which is associated with low plasma levels of the protein. Its prevalence in patients with venous thrombosis is also unknown. We investigated the frequency of the FXIII Val34Leu and FXII 46 C→T mutations in 189 patients with deep venous thrombosis and in 187 age-, gender- and race-matched controls. FXIII Val34Leu was detected in 38.6% of the patients and in 41.2% of the controls. Interestingly, homozygosity for the FXIII mutation was found in 1.6% of the patients and in 9.6% of the controls. yielding an odds ratio (OR) for venous thrombosis of 0.16 (95% CI: 0.05-0.5). The OR for heterozygotes was 1.1 (95% CI: 0.7-1.7). The FXII 46 C→T mutation was detected in 46.0% of the patients and in 48.6% of the controls. The OR for heterozygotes was 0.9 (95% CI: 0.6-1.4) and for homozygotes the OR was 0.8 (95% CI: 0.3-1.9). Our data indicate that the FXII 46 C→T mutation is unlikely to be a major risk factor for venous thrombotic disease. In contrast, the homozygous state for FXIII Val34Leu is a strong protective factor against venous thrombosis, which emerges as a novel generic factor involved in the aetiology of thrombophilia.

Orientation, building height and sky view factor as energy efficiency design parameters

The energy efficiency of buildings should be a goal at the pre-design phase, though the importance of the design variables is often neglected even during the design process. Highlighting the relevance of these design variables, this research studies the relationships of building location variables with the electrical energy consumption of residential units. The following building design parameters are considered: orientation, story height and sky view factor (SVF). The consideration of the SVF as a location variable contributes to the originality of this research. Data of electrical energy consumption and users' profiles were collected and several variables were considered for the development of an Artificial Neural Network model. This model allows the determination of the relative importance of each variable. The results show that the apartments' orientation is the most important design variable for the energy consumption, although the story height and the sky view factor play a fundamental role in that consumption too. We pointed out that building heights above twenty-four meters do not optimize the energy efficiency of the apartments and also that an increasing SVF can influence the energy consumption of an apartment according to their orientation.

Test results of fault current limiter using YBCO tapes with shunt protection

A Fault Current Limiter (FCL) based on high temperature superconducting elements with four tapes in parallel were designed and tested in 220 V line for a fault current peak between 1 kA to 4 kA. The elements employed second generation (2G) HTS tapes of YBCO coated conductor with stainless steel reinforcement. The tapes were electrically connected in parallel with effective length of 0.4 m per element (16 elements connected in series) constituting a single-phase unit. The FCL performance was evaluated through over-current tests and its recovery characteristics under load current were analyzed using optimized value of the shunt protection. The projected limiting ratio achieved a factor higher than 4 during fault of 5 cycles without degradation. Construction details and further test results will be shown in the paper. © 2010 IOP Publishing Ltd.

Rendimento e qualidade de melancia cultivada sob proteção de agrotêxtil combinado com mulching plástico

The ground cover is an agricultural practice that is designed mainly to control weeds, while the nonwoven prevents the attack of pests. The objective of this study was to evaluate the yield and fruit quality of watermelon grown under nonwoven textile protection combined with plastic mulching. The experiment was conducted at the WG Fruits Company, Baraúna, RN. A randomized block design was used in a factorial 3 x 3 + 1 with four replications, and 36 plants per plot, with the first factor of color of plastic used in mulching (black, silver and white) and the second factor nonwoven textile with different times of use (first and third crop nonwoven under textile and without nonwoven) and an absolute control (no mulching and without nonwoven). The production variables evaluated were: yield (commercial, noncommercial, and total fruit yield), and fruit quality (soluble solids - SS, titratable acidity - TA and SS/TA). The use of mulching and protection of plants with nonwoven textile provided a higher yield of watermelon fruits in relation to bare soil with and without nonwoven textile protection. Fruit quality was not affected by the use of mulching and nonwoven.

Selective protection of the cerebellum against intracerebroventricular LPS is mediated by local melatonin synthesis

Although melatonin is mainly produced by the pineal gland, an increasing number of extra-pineal sites of melatonin synthesis have been described. We previously demonstrated the existence of bidirectional communication between the pineal gland and the immune system that drives a switch in melatonin production from the pineal gland to peripheral organs during the mounting of an innate immune response. In the present study, we show that acute neuroinflammation induced by lipopolysaccharide (LPS) injected directly into the lateral ventricles of adult rats reduces the nocturnal peak of melatonin in the plasma and induces its synthesis in the cerebellum, though not in the cortex or hippocampus. This increase in cerebellar melatonin content requires the activation of nuclear factor kappa B (NF-κB), which positively regulates the expression of the key enzyme for melatonin synthesis, arylalkylamine N-acetyltransferase (AA-NAT). Interestingly, LPS treatment led to neuronal death in the hippocampus and cortex, but not in the cerebellum. This privileged protection of cerebellar cells was abrogated when G-protein-coupled melatonin receptors were blocked by the melatonin antagonist luzindole, suggesting that the local production of melatonin protects cerebellar neurons from LPS toxicity. This is the first demonstration of a switch between pineal and extra-pineal melatonin production in the central nervous system following a neuroinflammatory response. These results have direct implications concerning the differential susceptibility of specific brain areas to neuronal death.

Thermoregulatory responses of goats in hot environments

Notwithstanding the solar radiation is recognized as a detrimental factor to the thermal balance and responses of animals on the range in tropical conditions, studies on the amount of thermal radiation absorbed by goats therein associated with data on their production and heat exchange are still lacking. Metabolic heat production and the heat exchange of goats in the sun and in the shade were measured simultaneously, aiming to observe its thermal equilibrium. The results showed that black goats absorb twice as much as the white goats under intense solar radiation (higher than 800 W m(-2)). This observation leads to a higher surface temperature of black goats, but it must not be seen as a disadvantage, because they increase their sensible heat flow in the coat-air interface, especially the convection heat flow at high wind speeds. In the shade, no difference between the coat colours was observed and both presented a lower absorption of heat and a lower sensible heat flow gain. When solar radiation levels increases from 300 to 1000 W m(-2), we observed an increase of the heat losses through latent flow in both respiratory and cutaneous surface. Cutaneous evaporation was responsible for almost 90 % of the latent heat losses, independently of the coat colour. Goats decrease the metabolic heat production under solar radiation levels up to 800 W m(-2), and increase in levels higher than this, because there is an increase of the respiratory rate and of the respiratory flow, but the fractions of consumed oxygen and produced carbon dioxide are maintained stable. The respiratory rate of black goats was higher than the white ones, under 300 W m(-2) (55 and 45 resp min(-1)) and 1000 W m(-2) (120 and 95 resp min(-1), respectively). It was concluded that shade or any protection against solar radiation levels above 800 Wm(-2) is critical to guarantee goat's thermal equilibrium. Strategies concerning the grazing period in accordance with the time of the day alone are not appropriate, because the levels of radiation depend on the latitude of the location.

Genetic erosion risk by environmental and antropic factor applied on strategies for Lychnophora ericoides conservation

This study evaluated the genetic erosion risk factors and the strategic points for the conservation of Lychnophora ericoides population in “Paraíso Perdido” farm, Serra da Canastra (20° 37’ 54” S; 46° 19’ 37” W; 833 m height) in São João Batista do Glória City, Minas Gerais State, Brazil. The number of young and adult plants, the soil and the phenology were evaluated in two sample areas of 125 m2. Information about the species utilization was obtained with local informants. Data on the region were obtained through literature review, in loco evaluation, GPS and geo-referenced map. In addition, local use of the plant for mixtures of drug was evaluated. According to the results obtained, the soil of the population is lithic with a weathered portion of frank-sandy texture, very acidic and dystrophic. The population density is 0.16 individuals/m2, 0.078 young/adult plant. The predominant phenophase was fruiting (100% plants) followed by flowering (21.62% plants). The local community uses the leaves of the plant in the form of hydroalcoholic extracts, as anti-inflammatory. Based on the evaluated parameters, the population is at 73% risk of genetic erosion. The detected key points were the development of activities including the participation of the local community for habitat protection as well as germplasm collection, seedlings production and reintroduction, together with environmental education, supervision, and reduction in the propensity for fire.