Proinflammatory profile of in vitro monocytes in the ageing is affected by lymphocytes presence

Background: Aging is associated with complex and constant remodeling of the immune function, resulting in an increasing susceptibility to infection and others diseases. The infections caused by Gram-negative microorganisms, present in nursing homes and hospitals, constitute one of the most common infections in the elderly, and are mainly combated by innate immune cells. Although the functions of innate immunity seem more preserved during aging than of adaptive immune mechanisms, two systems operate in an integrated way in the body, so that injury in one part of the immune system inevitably affects the other as they are part of a defensive network. The aim of this study was to investigate the in vitro production of proinflammatory (TNF-α, IL-6, IL-1β, CXCL-8 and MCP-1) and anti-inflammatory (TGF-β and IL-10) cytokines by monocytes, stimulated or not (basal) with lipopolysaccharide, from healthy young and elderly subjects. By means of PBMCs, we also studied if cytokine profile is altered in these different patient groups, in the presence of lymphocytes, under the same experimental conditions.Results: The monocytes from elderly presented higher basal production of TNF-α, MCP-1 and lower of TGF-β than young monocytes. PBMC showed similar cytokines production, irrespective age or stimulation presence. In the presence of lymphocytes, the spontaneous production of IL-10 was higher and of TGF-β was lower than monocytes, regardless of age. After LPS-stimulation, the presence of lymphocytes resulted in increased IL-6, IL-1β, MCP-1 and IL-10 and decreased CXCL-8 and TGF-β in comparison to pure culture of monocytes from young patients. With age, the same differences were observed, except for CXCL-8 and TGF-β which production was the same between monocytes and PBMC stimulated with LPS.Conclusion: These findings reinforce the systemic state of inflamm-aging frequently reported in elderly and considered a factor of susceptibility to numerous diseases. Still, the cytokine production from just monocytes of the elderly showed alterations, while in the lymphocyte presence not, suggesting an immunomodulator role of lymphocytes on monocytes. In addition, the differences between the production patterns by LPS-stimulated PBMC between young and elderly volunteers can be related with an imbalance in response against Gram-negative bacteria in throughout life. © 2013 Pinke et al.; licensee BioMed Central Ltd.

An approach to local immunotoxicity induced by adjuvanted vaccines

The occurrence of injection site reactions following immunization is the most frequently reported toxicity manifestation of vaccines; however, the different types of local reactions and the different mechanisms involved are still unclear. Here, the current advances in adjuvants and the role that adjuvants play in local reactions are reviewed. The role of adjuvants in the formation of the loco-regional complex (LRC), which consists of the injection site, draining lymphatic vessels and regional lymph nodes, is also discussed. Finally, strategies and recommendations for the rational design of adjuvanted vaccines are discussed, with a particular interest in the reduction of local inflammation. © 2013 Elsevier B.V.

Efeito da própolis in vivo sobre a expressão de receptores semelhantes a Toll (TLR-4 e TLR-2) e produção de citocinas por camundongos BALB/c

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Síntese em fase sólida e estudos comformacionais de análogos contendo TOAC de um peptídeo de rã Hypsiboas albopunctatus

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito da terapia antituberculose na expressão dos receptores TLR-2 e 4, do fator de transcrição Foxp3, da Óxido Nítrico Sintase Induzível, no perfil de citocinas e nas alterações genóticas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Mensuração do superóxido e apoptose neutrofílica em cães azotêmicos e urêmicos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Pesquisa de mutação do gene predito da lectina ligante de manose em cães: comparação entre dois alvos para diagnóstico molecular de leishmaniose visceral canina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

β Defensinas em membranas corioamnióticas de gestações complicadas por prematuridade: expressão gênica e imunolocalização

Pós-graduação em Patologia - FMB

Avaliação do crescimento e da atividade imune de tilápias alimentadas com dietas suplementadas com unha de gato (Uncaria tomentosa)

Pós-graduação em Aquicultura - FCAV

Caracterização da resposta imune celular em mulheres com câncer de ovário

Pós-graduação em Ginecologia, Obstetrícia e Mastologia - FMB

Imunomodulação por levamisol na imunidade inata e adquirida de pacu (Piaractus mesopotamicus)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Imunoterapia com a vacina heteróloga de DNA-HSP65+proteína-HSP65 de Mycobacterium leprae em tumores mamários de cadelas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação da atividade antiinflamatória, antitumoral e antiangiogênica de compostos isolados da planta Alchornea glandulosa e de fungos endofíticos a ela relacionados

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Aspectos clínicos, microbiológicos e da regulação da imunidade inata na vaginose bacteriana

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O papel de receptores de imunidade inata βGR, MR, TLR2 e TLR4 no reconhecimento da Candida albicans por monócitos humanos estimulados com polissacarídeos extraídos do cogumelo Agaricus brasiliensis

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)