328 resultados para Microbiota


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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Odontologia - FOA

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Effects of bentazon, metolachlor, trifluralin, imazethapyr, imazethapyr+lactofen, haloxyfop-methyl, glyphosate and chlorimuron-ethyl at rates of 2 and 10 times the equivalent commercial dose on soil microbial activity was evaluated in soil samples extracted from a field never treated before. Global soil microbe respiration, estimated by CO2 production at 2, 4, 8, 12, 16, 20, 24 and 28 days of soil incubation and enzymatic activities (dehydrogenase and fluorescein diacetate hydrolysis) at 8 and 28 days were used as bioindicators. Bentazon and mixture imazethapyr+lactofen at the highest rate and haloxyfop-methyl at both rates, inhibited soil respiration although with differences in timing and duration. None of the herbicides affected FDA hydrolysis. Dehydrogenase activity was inhibited at 8 days of incubation with bentazon and imazethapyr at high rates but it was stimulated by metolachlor and imazethapyr at low rate and glyphosate at the highest rate. Herbicide effects on soil microbial activity was detected with higher sensitivity by global soil microbe respiration and dehydrogenase activity than by FDA hydrolysis. Only dehydrogenase activity and soil respiration estimations at 8 days of soil incubation had significant correlation. Results indicated the need of multiple estimations when evaluating herbicides effects on soil microbiota

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Pós-graduação em Microbiologia Agropecuária - FCAV

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Staphylococcus are not usually studied in the oral cavity, when this happens, they are considered to belong to transitory microflora. Individuals that present periodontal disease represent possibles reservoirs of these opportunist bacteria in the oral cavity. The use of antibiotics whether for treatment of periodontal disease or due to hospital infections, may predispose the increase of the Staphylococcus spp. in the oral cavity because they easily become resistant to antibiotics, resulting in superinfection. The study was made with 88 patients, minimum age- 25 years old, presenting chronical periodontitis, with, at least, two sites having a probing pocket bigger or equal to 5mm. After anamnese and clinical periodontal examination samples were taken from the periodontal pocket using paper cones and from the oral cavity using mouth rinse. Of the total patients 37,50% presented Staphylococcus spp. in the periodontal pocket and 61,36% in lhe oral cavity; 27,27% presented bacteria in the two sites, not necessarily of the same specie. S. epidermidis was the most prevailing specie in periodontal pocket (15,9%) and oral cavity (27,27%). Positive for S. aureus in the periodontal pocket were 4,5% and for the oral cavity 25%, and 3,4% were positive for the two sites. There was not found significative statistical difference referring to the presence of the microorganisms as to age, smoking habit and increase of the probing depth. The majority of the isolated Staphylococcus samples showed resistance to the tested antibiotics, indicating that the drugs as an adjunct to periodontal therapy, must be seen with caution