The production and potential of biofertilizers to improve crop yields

Extensive interactions of plant roots with soil microorganisms affect plant nutrition either directly by influencing mineral nutrient availability or indirectly through enhanced uptake efficiency via plant root growth promotion. Beneficial microbial interactions with roots may be either endophytic or associative and can be symbiotic, mutualistic, or incidental in nature. The increased understanding of the role of root – or rhizosphere –associated with microbes in the nutrition and/or yield of agricultural crops in particular has resulted in promotion of their use in agricultural production as alternatives or supplements to mineral or organic fertilizers. Despite this, there is an obvious lack of market penetration of microbial inoculants. This review specifically focuses on microbial inoculants, collectively termed biofertilizers, used to improve nutrition and yields of grain, legume, oil, tuber, and other crops. A vast number of commercial biofertilizers are available worldwide; however, the quality and efficacy of many of them are not proven or tested. In the absence of efficacious biofertilizers of good and consistent quality, the dependence on the use of mineral fertilizers is not likely to decrease. Thus the availability of high-quality biofertilizers must be priority particularly in countries where crop plant production plays a key role in the economy and food security.

Plant extract assisted extracellular tannase production by Aspergillus sp MIK 23

An extracellular tannase (E.C. 3.1.1.20) producing fungal strain was isolated from soil and identified as Aspergillus sp MIK23. Out of various plant extracts, Terminalia chebula powder (TCP) in the optimized medium enhanced enzyme production. Maximum yield of tannase (3 IU ml-1) was obtained with glucose (10 g/L), urea (2 g/L), and yeast extract (2.5 g/L) when inoculated with 10% inoculum in 48 h. An initial medium at pH 6.0 and a cultivation temperature of 37 0C was found to be optimum for enzyme production. Metal ions Mg2+, Zn2+, Ca2+, Cu2+ and Cd2+ did not improve enzyme activity, whereas, Ca2+, Fe2+ and Hg2+ repressed enzyme activity. The enzyme was purified using ammonium sulfate precipitation followed by Q-sepharose ion-exchange chromatography. The enzyme was purified to 42-fold with an overall recovery of 20. The pH and temperature optima of the purified tannase were found to be 7.0 and 37°C, respectively.

Use of the wound-inducible NtQPT2 promoter from Nicotiana tabacum for production of a plant-made vaccine

The wound-inducible quinolinate phosphoribosyl transferase promoter from Nicotiana tabacum (NtQPT2) was assessed for its capacity to produce B-subunit of the heat-labile toxin (LTB) from enterotoxigenic Escherichia coli in transgenic plant tissues. Comparisons were made with the widely used and constitutive Cauliflower Mosaic Virus 35S (CaMV35S) promoter. The NtQPT2 promoter produced somewhat lower average concentrations of LTB protein per unit weight of hairy root tissue but allowed better growth thereby producing similar or higher overall average yields of LTB per culture batch. Transgenic tobacco plants containing the NtQPT2-LTB construct contained LTB protein in roots but not leaves. Moreover, wounding NtQPT2-LTB transgenic plants, by removal of apices, resulted in an approximate 500% increase in LTB levels in roots when analysed several days later. CaMV35S-LTB transgenic plants contained LTB protein in leaves and roots but wounding made no difference to their LTB content.

Estimating quality costs in an automotive stamping plant through the use of simulation

The pressures of modern manufacturing require that the quality-cost benefits are determined when evaluating new procedures or alternative operating policies. Traditionally, cost reports and other quality metrics have been used for this purpose. However, the interactions between the main quality cost drivers cannot be understood at the superficial level and the effect that a new process or an alternative operating policy may have on quality costs is difficult to determine. An alternative to the traditional costing methods is simulation. The current work uses simulation to evaluate quality costs in an automotive stamping plant where the quality control is determined by operator inspection of their own work. Self-inspection quality control provides instantaneous feedback of quality problems, allowing for quick rectification. However, the difficult nature of surface finish inspection of automotive panels can create inspection and control errors. A simulation model was developed to investigate the cost effects of inspection and control errors and it was found that inspection error had a significant effect in increasing total quality cost, with the magnitude of this increase dependent on the level of control. Further, the simulation found that the lowest cost quality control policy was that which allowed a number of defective panels to accumulate before resetting the press-line.

The promise of plant based foods - new directions?

The consumption of a plant based diet has important implications for health and the environment, yet little is known about the attitudes of the food industry and media personnel towards plant foods and the barriers they face to the production, retail and promotion of such foods. Therefore, 32 Australian food industry and media representatives participated in individual interviews on plant foods. Participants saw strong health benefits associated with the consumption of plant foods. Barriers to production or retail of plant foods that were identified included drought and lack of grower control over the distribution and marketing of their product. Participants believed that we are in the early stages of learning about consumer attitudes and preferences to plant foods. In addition to the greater financial resources necessary to be able to compete with less healthy foods, better cooperation within and between industry and non-industry groups was identified as a key factor that could help in their endeavours to promote plant foods.

Food industry awareness of consumers` plant food beliefs

Abstract: Purpose – The aims of this study were to examine farmers' and food processors' alignment with consumers' views about plant foods and their intentions to produce plant foods. Design/methodology/approach – Data on plant food beliefs were collected from mail surveys of farmers, food processing businesses and random population samples of adults in Victoria, Australia. Findings – There were strong differences between consumers' beliefs and farmers' and food processors' perceptions of consumers' beliefs. For example, a higher proportion of farmers and processors believed that consumers would eat more plant foods if more convenience-oriented plant-based meals were available than consumers themselves agreed. Farmers appeared to be more aware of or aligned with consumers' beliefs than were processors. One- and two-thirds of farmers and processors respectively were planning to grow or process more plant foods, which bodes well for the availability of plant and plant-based foods. Research limitations/implications – Study limitations include the small food industry sample sizes and possible response bias, although analysis suggests the latter was low. Future research could survey a larger sample of food industry representatives, including those from other sectors (e.g. retailers). Practical implications – Education of consumers and industry groups on plant foods and better lines of communication from consumer to processor to farmer, are required. Originality/value – To the authors' knowledge, this is the first study to examine farmers' and food processors' awareness of consumers' beliefs about plant foods. This issue is important for those involved with the production and marketing of plant foods or with food, farming and health policy.

Simulating the change from a push to pull production system

This paper will document the initial discrete-event simulation performed to study a proposed change from a push to a pull system in an existing manufacturing company. The system is characterised by five machine lines with intermediate buffers, and five major part groupings. A simulation model has been developed to mimic the flow of kanban cards in the physical system, by using a series of requests that propagate back through the facility, which the machines must respond to. The customer
demand therefore controls the level of activity in the plant. The results of the initial modelling steps will be presented in this paper, especially the impact of kanban lot size and demand variability on the output and stability of the production system, from which a set of future work is proposed.

Analysis of stamping production data with view towards quality management

A quality analysis trial was undertaken at Ford Geelong Stamping Plant on a press line that was fitted with standard press sensors to measure press and binder force over the stamping cycle for each panel. The quality of randomly sampled panels was measured by obtaining the panel thicknesses at five points, for 135 panels in total. These points were chosen such that they exhibited different forming modes. This paper analyses the input force data and the output quality data from the trial to determine any potential relationships. The analysis of the production data was performed using statistical correlation techniques to determine initial potential relationships between input and output variables. An Active Shape Model was used to extract features when identifying the major sources of variation within the input data. However, the initial analysis of the data elicited no direct relationship between the input variables measured and the panel thicknesses. This result is significant as the data collected is from a standard sensor configuration found in many press lines through-out the world. The reason for the lack of a direct relationship is believed to come from the lack of sensitivity in the force measurements which are not able to identify small changes in the process, whereas gross geometric variations have in previous studies shown an obvious relationship with changes in the force press profile. This means that existing force sensors require augmentation by additional sensors if a detailed automatic quality control system for the press lines based on input sensors alone.

Lignin production and monolignol pathway gene expression is suppressed by abscisic acid during defence reactions of Arabidopsis

The phytohormone, abscisic acid (ABA) has been shown to influence the outcome of the interactions between various hosts with biotrophic and hemibiotrophic pathogens. Susceptibility to avirulent isolates can be induced by addition of low physiological concentrations of ABA to plants. In contrast, addition of ABA biosynthesis inhibitors induced resistance following challenge of plants by virulent isolates. ABA deficient mutants of Arabidopsis, such as aba1-1, were resistant to virulent isolates of Peronospora parasitica. In interactions of Arabidopsis with avirulent isolates of Pseudomonas syringae pv. tomato, susceptibility was induced following addition of ABA or imposition of drought stress. These results indicate a pivotal, albiet undefined, role for ABA in determining either susceptibility or resistance to pathogen attack. We have found that the production of the cell wall strengthening compound, lignin, is increased during resistant interactions of aba1-1 but suppressed in ABA induced susceptible interactions. Using RT-PCR and microarray analysis we have found down-regulation by ABA of key genes of the phenylpropanoid pathway especially of those genes involved directly in lignin biosynthesis. ABA also down-regulates a number of genes in other functional classes including those involved in defence and cell signalling.

Suppression by abscisic acid of lignin production and monolignol pathway gene expression in interactions of Arabidopsis with oomycete and bacterial pathogens

The phytohormone, abscisic acid (ABA) has been shown to influence the outcome of the interactions between various hosts with biotrophic and hemibiotrophic pathogens. Susceptibility to avirulent isolates can be induced in plants by addition of low physiological concentrations of ABA. In contrast, addition of ABA biosynthesis inhibitors induced resistance following challenge of plants by virulent isolates. ABA deficient mutants of Arabidopsis, such as aba1-1, were resistant to virulent isolates of Peronospora parasitica. In interactions of Arabidopsis with avirulent isolates of Pseudomonas syringae pv. tomato, susceptibility was induced following addition of ABA or imposition of drought stress. These results indicate a pivotal, albiet undefined, role for ABA in determining either susceptibility or resistance to pathogen attack. We have found that the production of the cell wall strengthening compound, lignin, is increased during resistant interactions of aba1-1 but suppressed in ABA-induced susceptible interactions. Using RT-PCR and microarray analysis we have found down-regulation by ABA of key genes of the phenylpropanoid pathway especially of those genes involved directly in lignin biosynthesis. ABA also down-regulates a number of genes in other functional classes including those involved in defence and cell signalling.

Production decisions and lot sizing for automotive stamping

A discrete event model was used to examine the effect of machine downtime and operating policy on the long-run average cost of an automotive stamping line. Operating policy refers to the selection of a target batch size and the circumstances under which a line stoppage will lead to the current batch being abandoned. It is assumed that the abandon/resume decision is based solely on the severity of the problem (i.e., repair cost) and the fraction of the batch completed. A method of identifying low-cost operating policies is presented using data obtained from a real stamping plant. It is found that, within a single part framework, this approach results in significantly lower average costs than are currently achieved. It is also demonstrated that by varying the model parameters it is possible to measure the potential benefits arising from process modifications (e.g., decreased die-set times). This can be used to identify the areas where improvements will have the greatest impact on cost and is particularly useful when assessing the expected return on a potential investment. A multiple-part extension to the model is suggested and the potential benefits discussed.

Current knowledge of the environmental influences on Australian cashmere production and quality

Goat fibre production is affected to a similar extent by genetic and environmental influences. Environmental influences include bio-geophysical factors (photoperiod, climate-herbage system and soil-plant trace nutrient composition), country of origin, nutrition factors (live weight, growth patterns) and management factors (farm, herd age and sex structure). Nutrition and management influences discussed include rate of stocking, energy nutrition, live weight change, parturition and management during shearing. The nutritional variation within and among years is the most important climatic factor influencing cashmere production, fibre diameter and fibre curvature (crimp). With productive cashmere goats, large responses to energy supplementation have been measured with optimum nutritional management. The effects and importance of management and hygiene during fibre harvesting (shearing) in producing quality fibre are emphasised.

Impact of saltmarsh disturbance on seed quality of Sarcocornia (Sarcocornia quinqueflora), a food plant of an endangered Australian parrot

The article presents a study which evaluates the impact of saltmarsh perturbation on seed quality of Sarcocornia which is a food for the endangered Orange-bellied parrot in Australia. It notes the significant impact of grazing on the energy and availability of Sarcocornia seeds in saltmarshes and indicates that graze-free saltmarshes with regular inundation provide the highest potential for Sarcocornia seed availability. Further, the substantial factor of soil characteristics and salinity of flood water and inundation on the seed production of Sarcocornia is also discussed.

Modelling and optimising operations of a plant using JIT philosophy

An Australian automotive component company plans to assemble and deliver seats to a car manufacturer on a "just-in-time" basis at its new plant. The research objective was to model seat assembly operations and apply Toyota goal chasing algorithm and user defined algorithm to balance workload among all the assembly workstations and areas.