Isolating conifer DNA: a superior polysaccharide elimination method

Genomic DNA was isolated from frozen needles of maturePinus radiata clones using a modified extraction technique incorporating cetyltrimethylammonium bromide (CTAB) for cell lysis. A high sodium chloride concentration (2 M) was used at 2 stages of the extraction procedure to eradicate polysaccharides, yielding pure genomic DNA suitable for restriction enzyme digestion and PCR amplification. Extractions were scaled down to suit 1.5-mL Eppendorf tubes, allowing easier handling and enhanced sterility.

The charge effect of cationic surfactants on the elimination of fibre beads in the electrospinning of polystyrene

Polystyrene nanofibres were electrospun with the inclusion of cationic surfactants, dodecyltrimethylammonium bromide (DTAB) or tetrabutylammonium chloride (TBAC), in the polymer solution. A small amount of cationic surfactant effectively stopped the formation of beaded fibres during the electrospinning. The cationic surfactants were also found to improve the solution conductivity, but had no effect on the viscosity. Only DTAB had an effect on the surface tension of the polymer solution, the surface tension decreasing slightly with an increase in the concentration of DTAB.

The formation of beaded fibres was attributed to an insufficient stretch of the filaments during the whipping of the jet, due to a low charge density. Adding the cationic surfactants improved the net charge density that enhanced the whipping instability. The jet was stretched under stronger charge repulsion and at a higher speed, resulting in an exhaustion of the bead structure. In addition, a polymer/surfactant interaction was found in the polystyrene–DTAB solution system, while this interaction was not found in the polystyrene–TBAC system. The polymer/surfactant interaction led to the formation of thinner fibres than those formed in the absence of the interaction.

The effects of a non-ionic surfactant, Triton X-405, on the electrospun fibres were also studied. The addition of Triton X-405 did not eliminate the fibre beads, but reduced the bead numbers and changed the morphology. Triton X-405 slightly improved the solution conductivity, and had a minor effect on the surface tension, but no effect on the viscosity.

Use of pineapple juice for elimination of egg stickiness of common carp (Cyprinus carpio L.)

Four treatments including three different concentrations of pineapple solution (I %, 3%, 5%) and the traditional method (salt/urea/tannin solutions) were used to eliminate egg stickiness of common carp. With the traditional method, fertilization and hatching rates were 74.5% ± 1.2 and 70.2% ± 1.1, respectively. The highest fertilization rate (89.3% ± 0.7) and hatching rate (86.6% ± 1.4) were found with treatment of 1% pineapple juice solution and was significantly different from those obtained with other treatments (P<0.05). Using pineapple juice for desticking common carp eggs also reduced the time of egg handling from almost one hour required with the traditional method, to about three minutes, as well as increasing fertilization and hatching rate under hatchery conditions.

Single transferable vote with Borda elimination: proportional representation, moderation, quasi-chaos and stability

Modifying single transferable vote (STV) by removing candidates according to their Borda scores creates a new vote counting system (STV-B) that is not quasi-chaotic, results in proportional representation, and promotes the election of moderate candidates. Dummett, M.A.E. [1997. Principles of Electoral Reform. Oxford University, New York] noted quasi-chaos in STV and proposed the “Quota/Borda system” (QBS) as a solution. STV-B and QBS retain proportional representation from STV, permit some influence on candidate selection to occur between voting blocks thereby promoting moderate results, and are much more stable than STV when subjected to small changes in voter preferences. Under STV-B, and not QBS, a minority that shares some preferences may elect a candidate even if the minority is not a solid coalition.

GAD2 on chromosome 10p12 is a candidate gene for human obesity

The gene GAD2 encoding the glutamic acid decarboxylase enzyme (GAD65) is a positional candidate gene for obesity on Chromosome 10p11–12, a susceptibility locus for morbid obesity in four independent ethnic populations. GAD65 catalyzes the formation of γ-aminobutyric acid (GABA), which interacts with neuropeptide Y in the paraventricular nucleus to contribute to stimulate food intake. A case-control study (575 morbidly obese and 646 control subjects) analyzing GAD2 variants identified both a protective haplotype, including the most frequent alleles of single nucleotide polymorphisms (SNPs) +61450 C>A and +83897 T>A (OR = 0.81, 95% CI [0.681–0.972], p = 0.0049) and an at-risk SNP (−243 A>G) for morbid obesity (OR = 1.3, 95% CI [1.053–1.585], p = 0.014). Furthermore, familial-based analyses confirmed the association with the obesity of SNP +61450 C>A and +83897 T>A haplotype (χ2 = 7.637, p = 0.02). In the murine insulinoma cell line βTC3, the G at-risk allele of SNP −243 A>G increased six times GAD2 promoter activity (p < 0.0001) and induced a 6-fold higher affinity for nuclear extracts. The −243 A>G SNP was associated with higher hunger scores (p = 0.007) and disinhibition scores (p = 0.028), as assessed by the Stunkard Three-Factor Eating Questionnaire. As GAD2 is highly expressed in pancreatic β cells, we analyzed GAD65 antibody level as a marker of β-cell activity and of insulin secretion. In the control group, −243 A>G, +61450 C>A, and +83897 T>A SNPs were associated with lower GAD65 autoantibody levels (p values of 0.003, 0.047, and 0.006, respectively). SNP +83897 T>A was associated with lower fasting insulin and insulin secretion, as assessed by the HOMA-B% homeostasis model of β-cell function (p = 0.009 and 0.01, respectively). These data support the hypothesis of the orexigenic effect of GABA in humans and of a contribution of genes involved in GABA metabolism in the modulation of food intake and in the development of morbid obesity.

Single transferable vote with Borda elimination : a new vote counting system

Dummett (1997) notes particular difficulties with single transferable vote (STV) and proposes an alternative vote counting system called "Quota/Borda system" (QBS) to remedy specific difficulties. I propose an alternative system, structurally related to QBS, which accomplishes similar solutions but has some significant differences. This alternative system is identical to STV in all aspects except one. It eliminates candidates in reverse order of their Borda scores rather than by their current ranking of first-place votes. I designate this system STV with Borda elimination (STV-B). STV-B and QBS share general features. They retain proportional representation from STV. However, they differ from STV is two critical manners. First, both permit some influence on candidate selection to occur between voting blocks. Second, they are much more stable than STV when subjected to small changes in voter preferences. Outcomes from STV-B differ from QBS outcomes in two ways. Under STV-B, a minority that shares some preferences may elect a candidate even if the minority is not a solid coalition, as is required for minorities under QBS. Further, QBS always selects Borda winners, either for a minority or overall. STV-B may reject a Borda winner through emphasis on each voter's most preferred candidates.

Graph matching : fast candidate elimination using machine learning techniques

Graph matching is an important class of methods in pattern recognition. Typically, a graph representing an unknown pattern is matched with a database of models. If the database of model graphs is large, an additional factor in induced into the overall complexity of the matching process. Various techniques for reducing the influence of this additional factor have been described in the literature. In this paper we propose to extract simple features from a graph and use them to eliminate candidate graphs from the database. The most powerful set of features and a decision tree useful for candidate elimination are found by means of the C4.5 algorithm, which was originally proposed for inductive learning of classication rules. Experimental results are reported demonstrating that effcient candidate elimination can be achieved by the proposed procedure.

Sex-dependent selection differentially shapes genetic variation on and off the guppy Y chromosome

Because selection is often sex-dependent, alleles can have positive effects on fitness in one sex and negative effects in the other, resulting in intralocus sexual conflict. Evolutionary theory predicts that intralocus sexual conflict can drive the evolution of sex limitation, sex-linkage, and sex chromosome differentiation. However, evidence that sex-dependent selection results in sex-linkage is limited. Here, we formally partition the contribution of Y-linked and non-Y-linked quantitative genetic variation in coloration, tail, and body size of male guppies (Poecilia reticulata)—traits previously implicated as sexually antagonistic. We show that these traits are strongly genetically correlated, both on and off the Y chromosome, but that these correlations differ in sign and magnitude between both parts of the genome. As predicted, variation in attractiveness was found to be associated with the Y-linked, rather than with the non-Y-linked component of genetic variation in male ornamentation. These findings show how the evolution of Y-linkage may be able to resolve sexual conflict. More generally, they provide unique insight into how sex-specific selection has the potential to differentially shape the genetic architecture of fitness traits across different parts of the genome.

Localization with ghost elimination of emitters via time-delay-of-arrival measurements

This paper investigates the theoretical requirements for unique localization of multiple emitters using time delay of arrival(TDoA) subjected to the data-association problem. Specifically, an examination is carried out to find the necessary fundamental requirements to solve the so-called ghost node problem pertaining to sensor arrays.

Localization with ghost elimination of emitters via time-of-arrival measurements

This paper looks at the theoretical conditions underpinning unique localization of synchronized multiple emitters using Time-of-Arrival measurements subjected to the data-association problem. The necessary fundamental requirements to solve the so-called ghost node problem associated with sensor arrays are examined. We derive a measurement bound for ideal situations and the underlying concepts are illustrated via simulations.