5 resultados para computational analysis, microarray design, transcript profiling, vascular tissues, white spruce (Picea glauca)

em Dalarna University College Electronic Archive


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In this study a case study was conducted at the Transport Agency. The government agency is seeking better alternatives to its current ways of keeping track of important dates stated in legal documents. The agency wants to explore the possibility for an IT-solution for keeping track on those dates. In the case study change analysis was conducted on the vehicle type approval process. Qualitative interviews were conducted with a section manager, vehicle type and component approvers, and with a system administrator and a system manager at the Transport Agency in Borlänge. The study describes an information environment in an organization in which date-controlled conditions apply and challenges with such an environment.The study also provides a proposal for designing an IT-solution for such an environment. The result that emerged showed that the information environment in terms of the legal documents and the institutions’ (EU and UNECE) websites where they are distributed is challenging for keeping track on dates. The challenges of the websites are that they are limited and complicated in terms of information search. The challenges with the legal documents are that date-controlled conditions apply. In other words, the dates themselves determine which dates and what applies and not. The legal documents are also many, retroactive, refer to each other and contain regulations for different kinds of areas, which makes difficult to find the relevant dates. The design proposal that emerged was made based on the existing IT environment at the Transport Agency. It was proposed that important key concepts are identified, categorized and realized in a database with searchability on the most important common concepts as denominators, and that it the information conforms with E-message. The developed database was based on date, date type, vehicle category, legal document, minor version and other info. A web interface was created in ASP.NET and C # for access to the database, with the message that a webbbased IT-solution should be considered.

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Böcker är, trots ett generellt minskat intresse, fortfarande en viktig del av vårtkulturella samhälle. Att böckers utseende anpassas efter sin målgrupp är ingetovanligt men frågan är om även könet på författaren spelar in gällandeutformningen av omslaget.Syftet med studien var därför att genom en visuell innehållsanalys av böckerspocketomslag kartlägga de skillnader som återfinns på manliga respektivekvinnliga författares omslag.Denna undersökning har baserats på en visuell innehållsanalys där 200pocketomslag analyserades, 100 från svenska författare och 100 från engelskaförfattare. Dessa var därefter jämlikt fördelade mellan könen. I analysenreflekteras det över pocketböckernas motiv, dominerande färg, typsnittsfamiljersamt typsnittsfärger.Resultatet från undersökningen tyder på att kvinnliga författare har ettfemininare utseende på sina böcker överlag, och att svenska kvinnligaförfattares omslag uppvisade flest tecken på en femininare utformning. Det gårdock inte att utesluta att böckernas genre kan ha ett inflytande gällandeböckernas utformning, då det var svårt att precisera genren på många böckeröverlag.

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För att ge insikt hur design för ett nytt rockalbum ska ske och ge information kring vad som bör finnas med på ett nytt omslag har denna studie utförts. Studien framförde information om att albumsgrafik var relevant. Först sades det att en nedåtgående trend gick att anta, eftersom den digitala musikbranschen tog över från den fysiska. Men det visade sig därefter vara precis lika stor försäljning på de fysiska albumen som de digitala. För att besvara målen i studien utfördes en visuell innehållsanalys och informationssökning på internet. Studiens syfte var att visuellt granska rockalbum från tre olika ursprungsländer, som var Sverige, Storbritannien och USA. Genom analysen kategoriserades designelement, teman och framtogs landstypiska albumomslag. Studien berörde även vem som styr designen av ett omslag samt ifall det kunde ses någon tongivande designer bland de album som ingick i analysen. I slutsatsen framgick det att Storbritannien och USA hade mycket lika grafiskt manér och att omslag från Sverige såg annorlunda ut. Det framgick även att banden styr utseendet på omslaget mer än designern, och från analysen framgick inte någon tongivande designer.

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Sustainable methods are required to protect newly planted tree seedlings from insect herbivore attack. To this end, here Norway spruce (Picea abies (L.) Karst.) seeds were treated with 2.5 mM nicotinamide (NIC), 2.5 mM nicotinic acid (NIA), 3 mM jasmonic acid (JA) or 0.2 mM 5-azacytidine (5-Aza), and 6-month-old seedlings grown from these seeds were planted at a reforestation area in central Sweden. Attack by pine weevils (Hylobius abietis) was reduced by 50 per cent by NIC treatment, 62.5 per cent by JA treatment and 25 per cent by 5-Aza treatment, when compared with seedlings grown from untreated seeds. Watering 18-month-old spruce seedlings with 2 mM NIC or 2 mM NIA did reduce attack during the first season in the field by 40 and 53 per cent, respectively, compared with untreated plants. Girdling was also reduced by the different treatments. Analysis of conifer seedlings treated with 5-Aza points at a possible involvement of epigenetic mechanisms in this defensive capacity. This is supported by a reduced level of DNA methylation in the needles of young spruce seedlings grown in a greenhouse from NIC-treated seeds. Seed treatment for seedling defense potentiation is simple, inexpensive and also a new approach for forestry with many potential applications.

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In boreal forest regions, a great portion of forest tree seedlings are stored indoors in late autumn to prevent seedlings from outdoor winter damage. For seedlings to be able to survive in storage it is crucial that they store well and can cope with the dark and cold storage environment. The aim of this study was to search for genes that can determine the vitality status of Norway spruce (Picea abies (L.) Karst.) seedlings during frozen storage. Furthermore, the sensitivity of the ColdNSure (TM) test, a gene activity test that predicts storability was assessed. The storability of seedlings was tested biweekly by evaluating damage with the gene activity test and the electrolyte leakage test after freezing seedlings to -25 A degrees C (the SELdiff-25 method). In parallel, seedlings were frozen stored at -3 A degrees C. According to both methods, seedlings were considered storable from week 41. This also corresponded to the post storage results determined at the end of the storage period. In order to identify vitality indicators, Next Generation Sequencing (NGS) was performed on bud samples collected during storage. Comparing physiological post storage data to gene analysis data revealed numerous vitality related genes. To validate the results, a second trial was performed. In this trial, gene activity was better in predicting seedling storability than the conventional freezing test; this indicates a high sensitivity level of this molecular assay. For multiple indicators a clear switch between damaged and vital seedlings was observed. A collection of indicators will be used in the future development of a commercial vitality test.