Molecules, wing pattern and distribution: an approach to species delimitation in the "loxurina group" (Lepidoptera: Lycaenidae: Penaincisalia)

The wide range of morphological variations in the “loxurina group” makes taxa identification difficult, and despite several reviews, serious taxonomical confusion remains. We make use of DNA data in conjunction with morphological appearance and available information on species distribution to delimit the boundaries of the “loxurina” group species previously established based on morphology. A fragment of 635 base pairs within the mtDNA gene cytochrome oxidase I (COI) was analysed for seven species of the “loxurina group”. Phylogenetic relationships among the included taxa were inferred using maximum parsimony and maximum likelihood methods. Penaincisalia sigsiga (Bálint et al), P. cillutincarae (Draudt), P. atymna (Hewitson) and P. loxurina (C. Felder & R. Felder) were easily delimited as the morphological, geographic and molecular data were congruent. Penaincisalia ludovica (Bálint & Wojtusiak) and P. loxurina astillero (Johnson) represent the same entity and constitute a sub-species of P. loxurina. However, incongruence among morphological, genetic, and geographic data is shown in P. chachapoya (Bálint & Wojtusiak) and P. tegulina (Bálint et al). Our results highlight that an integrative approach is needed to clarify the taxonomy of these neotropical taxa, but more genetic and geographical studies are still required.

Sampling scarab beetles in tropical forests: the effect of light source and night sampling periods

Light traps have been used widely to sample insect abundance and diversity, but their performance for sampling scarab beetles in tropical forests based on light source type and sampling hours throughout the night has not been evaluated. The efficiency of mercury-vapour lamps, cool white light and ultraviolet light sources in attracting Dynastinae, Melolonthinae and Rutelinae scarab beetles, and the most adequate period of the night to carry out the sampling was tested in different forest areas of Costa Rica. Our results showed that light source wavelengths and hours of sampling influenced scarab beetle catches. No significant differences were observed in trap performance between the ultraviolet light and mercury-vapour traps, whereas these two methods caught significantly more species richness and abundance than cool white light traps. Species composition also varied between methods. Large differences appear between catches in the sampling period, with the first five hours of the night being more effective than the last five hours. Because of their high efficiency and logistic advantages, we recommend ultraviolet light traps deployed during the first hours of the night as the best sampling method for biodiversity studies of those scarab beetles in tropical forests.

On nomenclature and identity of Scarabaeus aeruginosus Linnaeus, S. aeruginosus Drury and S. speciosissimus Scopoli (Coleoptera: Scarabaeoidea: Cetoniinae and Rutelinae)

The valid name for the largest European species of Cetoniinae is Protaetia speciosissima (Scopoli, 1786), with Protaetia aeruginosa (Medvedev, 1964) as a junior synonym. The specimen illustrated by Scopoli in the original description is designated as the lectotype of Scarabaeus speciosissimus Scopoli, 1786. Since the lectotype is lost, a neotype from Piedmont, Italy, is designated and deposited in the Museo Civico di Storia Naturale Carmagnola, Italy. The name Scarabaeus aeruginosus Drury, 1773 is unavailable since Drury did not describe a new species but misidentified Scarabaeus aeruginosus Linné, 1767. A specimen figured by Gronovius in 1764 and cited by Linné is designated as the lectotype of Scarabaeus aeruginosus Linné, 1767. This species remains dubious, but it can be assigned to the ruteline subtribe Anticheirina.

Elysia timida (Risso, 1818) three decades of research

During the last 30 years, studies on Elysia timida (Risso, 1818) have addressed various aspects related to food sources, photosynthetic efficiency of kleptoplasts, population genetics, chemical ecology and reproductive biology, both in the Mediterranean Sea and in the Mar Menor coastal lagoon. E. timida shows a strong specific interaction with Acetabularia acetabulum, retaining functional chloroplasts for at least 45 days and obtaining extra energy in periods when food resources are scarce. It shows control of parapodia, avoiding pigment photodestruction under oversaturated light conditions. The chemical ecological relationships established between E. timida and its potential predator fish, Thalassoma pavo, have also been evaluated, and it has been found that that the extracts of the mollusc contain repellent and unpalatable polypropionate compounds. Population genetics has demonstrated the genetic divergence between populations showing high and significant values of FST and genetic distances, and at least six privative alleles that are not shared with Mediterranean populations have been detected in lagoon populations. This sacoglossan is a poecilogonic species, and its lagoon populations show a greater reproductive output than Mediterranean populations; they produce a greater number of egg masses and embyros per individual, and the capsules have a wider diameter.

Polychaete distribution pattern on the Valencian Community coast, Spanish Mediterranean

Changes in benthic community structure are strongly related to environmental factors, and we need to determine how these natural changes occur in order to interpret the possible changes associated with anthropogenic impacts. The aim of this survey was to characterize and classify the polychaete assemblages inhabiting unpolluted soft bottoms in the Spanish Mediterranean in relation to environmental factors. Thirteen localities were sampled at depths between 9 and 31 m, from 2004 to 2006. Multivariate techniques showed that the structure of polychaete assemblages detected in 2004 was consistent over time and correlations between polychaetes and environmental factors were detected. The study area comprises four kinds of communities mainly characterized by polychaete assemblages, sediment types, and depth.

New Mediterranean Biodiversity Records (June 2012)

The present work reports on the extended distribution of nineteen species in the Mediterranean. These are: Upeneus pori (Fish:Turkey), Bursatella leachii (Mollusca, Opisthobranchia: eastern coast of Spain), Sparisoma cretense (Fish: Ionian coast of Greece), Pseudobryopsis myura (Chlorophyta:Turkey), Aplysia dactylomela (Mollusca, Opisthobranchia: Karpathos island, and Kyklades Archipelago, Greece), Asparagopsis armata and Botryocladia madagascariensis (Rhodophyta: South Peloponnesos, Greece), Oxynotus centrina (Fish: Greece), Caulerpa racemosa var. cylindracea (Chlorophyta ), Stypopodium schimperi (Phaeophyta ) Siganus luridus and Stephanolepis diaspros (Fish) Percnon gibbesi (Decapoda, Brachyura) (Kyklades Archipelago, Greece), Cerithium scabridum (Mollusca, Prosobranchia: Anavissos: Greece) and Cerithium renovatum (Mollusca, Prosobranchia: N. Κriti), Cassiopea andromeda (Scyphomedusa: Rhodos Island, Greece), Abra tenuis (Mollusca Bivalvia: Vouliagmeni Lake, Greece) Lagocephalus lagocephalus (Fish: Calabrian coast, Italy) and Plocamopherus ocellatus (Mollusca, Opisthobranchia: İskenderun Bay, Turkey).

Checking the concurrence among macrobenthic organism distribution patterns at different taxonomic scales in relation to environmental factors

The interaction of both natural conditions and anthropogenic environmental impacts can lead to different soft-bottom macrobenthic distribution patterns. Soft-bottom macrobenthic community was analysed at different taxonomic scales in order to evaluate whether diverse subset of organisms respond to the variability of the environmental pressures (natural and human induced) showing or not similar distribution patterns. Therefore, this long-term survey had been focused on a heterogeneous area, where both anthropogenic and natural stress may affect the community. Three perpendicular transects to the coast were established and stations at 4, 10 and 15 m depths were sampled at each transect twice a year (summer- winter) from 2004 to 2009. Non-parametric multivariate techniques were used to analyse soft-bottom macrobenthic community distribution and its relation to the environmental factors. Similar distribution patterns between investigated taxonomic levels were detected and they were mainly related to depth.

Colour morphotypes of Elysia timida (Sacoglossa, Gastropoda) are determined by light acclimation in food algae

Elysia timida (Risso, 1818) colonizing the shallow waters of the Mar Menor Lagoon (Spain) exhibit a brown and a green morph. It was hypothesised that these morphs were the result of feeding preferentially on brown and green algae, respectively. E. timida and its potential food sources, Acetabularia acetabulum (Chlorophyta) and Halopteris filicina (Heterokontophyta) were collected by snorkelling during April 2010. Photosynthetic pigments were analysed by HPLC, photo-physiological parameters were estimated by PAM fluorometry and body colour was characterized by spectral reflectance. Digital photography was used to count the number and area of red spots (small red dots on the slug’s surface) on the parapodia of the 2 morphs. In the laboratory, green E. timida was fed with A. acetabulum cultured under 2 light treatments (high light, 600 µmol E m−2 s−1 and low light, 40 µmol E m−2 s−1), and digital photography was used to monitor colour alterations in E. timida. Spectral reflectance confirmed the colour differences, but both morphs showed a pigment composition similar to the green alga A. acetabulum and showed none of the pigments present in the brown alga H. filicina, neither immediately after collection of the slugs in situ, nor after the feeding experiment. A. acetabulum grown under high light intensity changed from green to brown colour and E. timida changed to brown colour when fed with high-light acclimated A. acetabulum. Thus, E. timida colour differences could not be attributed to feeding on different algae groups but was likely the result of feeding on A. acetabulum growing under different light intensities.

Culturable aerobic and facultative bacteria from the gut of the polyphagic dung beetle Thorectes lusitanicus Jeckel

Unlike other dung beetles, the Iberian geotrupid Thorectes lusitanicus exhibits polyphagous behavior; for example, it is able to eat acorns, fungi, fruits, and carrion in addition to the dung of different mammals. This adaptation to digest a wider diet has physiological and developmental advantages and requires key changes in the composition and diversity of the beetle's gut microbiota. In this study, we isolated aerobic, facultative anaerobic, and aerotolerant microbiota amenable to grow in culture from the gut contents of T. lusitanicus and resolved isolate identity to the species level by sequencing 16S rRNA gene fragments. Using BLAST similarity searches and maximum likelihood phylogenetic analyses, we were able to reveal that the analyzed fraction (culturable, aerobic, facultative anaerobic, and aerotolerant) of beetle gut microbiota is dominated by the phyla Proteobacteria, Firmicutes and Actinobacteria. Among Proteobacteria, members of the order Enterobacteriales (Gammaproteobacteria) were the most abundant. The main functions associated with the bacteria found in the gut of T. lusitanicus would likely include nitrogen fixation, denitrification, detoxification, and diverse defensive roles against pathogens.

La utilización de los insectos en la gastronomía, un taller nutritivo

En la tercera edición del curso “Investigando la biodiversidad: presente y futuro” organizado por el CIBIO (Universidad de Alicante), se ha querido dar un enfoque aún más práctico a través de diferentes talleres en los que los alumnos han podido aprender y participar de manera activa mediante actividades como: la edición digital de fotografía de la naturaleza, la historia y elaboración de herberos y la utilización de los insectos en la gastronomía. Sobre este último taller versa este artículo que resume las actividades realizadas durante el mismo después de que en la primera parte del taller se impartieran conocimientos de distinta índole sobre esta actividad.

Supervivencia y probabilidad de recaptura del avión zapador en una colonia de Aspe: análisis del periodo 2004-2011

En este trabajo se analizan los anillamientos y recapturas de adultos en una colonia de avión zapador próxima a Aspe (Alicante) entre 2004 y 2011. El objetivo es estimar la variación de las tasas de supervivencia entre sexos y a lo largo del tiempo. La probabilidad de recaptura (p) muestra una tendencia a aumentar a lo largo del tiempo, pero de forma más marcada en los machos. La tasa de supervivencia difiere entre sexos, siendo ligeramente mayor en los machos. El mejor modelo incluye la variabilidad temporal de la supervivencia de machos, pero considera la de las hembras constante a lo largo del tiempo. La mayor variabilidad de la supervivencia de los machos es un patrón no detectado en estudios previos.

Evolution of Posidonia oceanica seagrass meadows and its implications for management

Results of the monitoring network of the Posidonia oceanica meadows in the Valencia region in Spain are analysed. For spatial comparison the whole data set has been analysed, however, for temporal trends we only selected stations that have been monitored at least 6 years in the period of 2002–2011 (26 stations in 13 localities). At the south of the studied area, meadows are larger, and they have higher density and covering than that in the Valencia Gulf, excluding Oropesa meadow. Monitoring of P. oceanica meadows in the Valencia region in Spain indicates that most of them are stationary or they are increasing their density and covering while no decline was observed in the studied meadows. These results indicate that there is not a general decline of P. oceanica meadows and that the decline of P. oceanica, when it has been observed in other studies, is produced by local causes that may be managed at the local level. This study also reflects the importance of long series of direct data to analyse trends in the population dynamics for slow-growing species.

Análisis y modelización de la actividad pesquera. La flota española en las aguas internacionales de la plataforma patagónica

Esta tesis está enfocada en el programa de observadores científicos en aguas del Atlántico Sudoccidental que el Instituto Español de Oceanografía (IEO) mantiene desde el año 1989 hasta la actualidad. Dicha base de datos ya ha sido empleada previamente por otros autores para estudiar los recursos pesqueros presentes, tanto en las aguas internacionales de la plataforma patagónica, más allá de las 200 millas náuticas de la costa argentina, como en la zona de pesca actualmente incluida dentro de la jurisdicción de las islas Falkland/Malvinas. Aquí nos centramos en el estudio de la actividad pesquera española que faena en la zona de aguas internacionales conocida como “División 46” y de los recursos a los que se dirige. Varios factores justifican la necesidad de esta tesis: (1) La necesidad de desarrollar una descripción detallada de la base de datos, no realizada hasta el momento. (2) Valorar la representatividad espacial del programa de observadores. (3) Establecer una serie de análisis espacio-temporales que describan la actividad pesquera de la flota española en estas aguas, (4) Desarrollar un completo y detallado análisis espacio-temporal de la distribución y abundancia de los recursos pesqueros a los que se dirige la flota. Por otra parte, las limitaciones propias de un programa de observadores científicos en buques pesqueros nos llevan, por necesidad, a explorar soluciones metodológicas que permitan un análisis fiable del esfuerzo pesquero y de la abundancia de los recursos. A este respecto, la disponibilidad de nueva información relacionada con las características físicas del área de estudio obtenida a raíz de las campañas oceanográficas realizadas en las aguas internacionales de la plataforma patagónica por el IEO y la Secretaría General del Mar (SGM) entre los años 2007 y 2011 y la disponibilidad de datos procedentes del Sistema de Localización de Buques de la SGM entre el periodo 2001 y 2007, nos ha permitido avanzar en el conocimiento de la actividad pesquera española en esta región. Como resultados principales, hemos definido algunas características fundamentales que determinan el marco de estudio de esta base de datos, hemos explorado diversas aproximaciones metodológicas que permitan un análisis de la representatividad de un programa de observadores en aguas internacionales, hemos desarrollado una descripción espacio-temporal de la estrategia de pesca en esta aguas y, a raíz del conocimiento adquirido, hemos realizado un análisis de los factores que afectan a la distribución de M. hubbsi y al esfuerzo de pesca invertido en su captura.

Breve apunte acerca de un manuscrito ornitológico redactado por Philip W. Munn durante su exilio en Portugal (1941-1945): The birds of the Balearic Islands (June, 1943)

Se presenta un bosquejo acerca de un documento de excepcional valor histórico para la ornitología balear: el manuscrito de Las Aves de las Islas Baleares (1943), redactado en inglés por el ornitólogo británico Philip Winchester Munn durante su exilio lisboeta.

José Antonio Valverde Gómez (1926-2003) y el quebrantahuesos (Gypaetus barbatus)

Hace diez años fallecía, en su domicilio sevillano, el naturalista vallisoletano José Antonio Valverde Gómez. En homenaje a su memoria, me propongo en este trabajo revisitar, y en lo posible también revisar, la relación que este biólogo mantuvo con nuestra ave más impresionante y bella, con esa joya de nuestro patrimonio natural y cultural, el quebrantahuesos. Para ello, se aporta documentación inédita, se añaden datos nuevos, y se corrigen viejos errores.