1 resultado para side effect
em Universidade Complutense de Madrid
Filtro por publicador
- Academic Archive On-line (Karlstad University; Sweden) (1)
- Academic Research Repository at Institute of Developing Economies (1)
- Adam Mickiewicz University Repository (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (3)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (2)
- Aquatic Commons (1)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (6)
- Archive of European Integration (1)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (1)
- Aston University Research Archive (22)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (17)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (7)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (2)
- Bioline International (1)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (48)
- Boston University Digital Common (3)
- Brock University, Canada (3)
- Bulgarian Digital Mathematics Library at IMI-BAS (1)
- CaltechTHESIS (1)
- Cambridge University Engineering Department Publications Database (6)
- CentAUR: Central Archive University of Reading - UK (26)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (21)
- Cochin University of Science & Technology (CUSAT), India (2)
- Coffee Science - Universidade Federal de Lavras (1)
- CORA - Cork Open Research Archive - University College Cork - Ireland (2)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (3)
- Deakin Research Online - Australia (27)
- Department of Computer Science E-Repository - King's College London, Strand, London (2)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (3)
- Digital Commons at Florida International University (4)
- DigitalCommons@The Texas Medical Center (6)
- DigitalCommons@University of Nebraska - Lincoln (1)
- Doria (National Library of Finland DSpace Services) - National Library of Finland, Finland (1)
- DRUM (Digital Repository at the University of Maryland) (2)
- Duke University (2)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (2)
- Glasgow Theses Service (2)
- Greenwich Academic Literature Archive - UK (1)
- Helda - Digital Repository of University of Helsinki (6)
- Indian Institute of Science - Bangalore - Índia (27)
- INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES (IPEN) - Repositório Digital da Produção Técnico Científica - BibliotecaTerezine Arantes Ferra (1)
- Instituto Politécnico de Bragança (1)
- Instituto Politécnico de Viseu (1)
- Instituto Politécnico do Porto, Portugal (2)
- Memoria Académica - FaHCE, UNLP - Argentina (3)
- National Center for Biotechnology Information - NCBI (8)
- Nottingham eTheses (1)
- Publishing Network for Geoscientific & Environmental Data (1)
- QSpace: Queen's University - Canada (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (14)
- Queensland University of Technology - ePrints Archive (464)
- ReCiL - Repositório Científico Lusófona - Grupo Lusófona, Portugal (1)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (1)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (1)
- Repositório digital da Fundação Getúlio Vargas - FGV (3)
- Repositório Institucional da Universidade de Aveiro - Portugal (1)
- Repositório Institucional da Universidade Federal de São Paulo - UNIFESP (1)
- Repositório Institucional da Universidade Federal do Rio Grande do Norte (1)
- Repositorio Institucional de la Universidad de Málaga (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (93)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (1)
- SAPIENTIA - Universidade do Algarve - Portugal (1)
- Savoirs UdeS : plateforme de diffusion de la production intellectuelle de l’Université de Sherbrooke - Canada (3)
- Scientific Open-access Literature Archive and Repository (1)
- Universidad del Rosario, Colombia (2)
- Universidad Politécnica de Madrid (15)
- Universidade Complutense de Madrid (1)
- Universidade de Lisboa - Repositório Aberto (2)
- Universidade Federal do Pará (1)
- Universidade Federal do Rio Grande do Norte (UFRN) (6)
- Universidade Metodista de São Paulo (3)
- Universitat de Girona, Spain (1)
- Université de Lausanne, Switzerland (1)
- Université de Montréal (1)
- Université de Montréal, Canada (10)
- Université Laval Mémoires et thèses électroniques (1)
- University of Connecticut - USA (1)
- University of Queensland eSpace - Australia (17)
- WestminsterResearch - UK (1)
Resumo:
Tagging of RNases, such as the ribotoxin α-sarcin, with the variable domains of antibodies directed to surface antigens that are selectively expressed on tumor cells endows cellular specificity to their cytotoxic action. A recombinant single-chain immunotoxin based on the ribotoxin α-sarcin (IMTXA33αS), produced in the generally regarded as safe (GRAS) yeast Pichia pastoris, has been recently described as a promising candidate for the treatment of colorectal cancer cells expressing the glycoprotein A33 (GPA33) antigen, due to its high specific and effective cytotoxic effect on in vitro assays against targeted cells. Here we report the in vivo antitumor effectiveness of this immunotoxin on nude mice bearing GPA33-positive human colon cancer xenografts. Two sets of independent assays were performed, including three experimental groups: control (PBS) and treatment with two different doses of immunotoxin (50 or 100 μg/ injection) (n = 8). Intraperitoneal administration of IMTXA33αS resulted in significant dose-dependent tumor growth inhibition. In addition, the remaining tumors excised from immunotoxin-treated mice showed absence of the GPA33 antigen and a clear inhibition of angiogenesis and proliferative capacity. No signs of immunotoxin-induced pathological changes were observed from specimens tissues.Overall these results show efficient and selective cytotoxic action on tumor xenografts, combined with the lack of severe side effects, suggesting that IMTXA33αS is a potential therapeutic agent against colorectal cancer.