121 resultados para Sequencing
Filtro por publicador
- Aberdeen University (4)
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- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (3)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (2)
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- Aquatic Commons (17)
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- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (1)
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- Aston University Research Archive (6)
- B-Digital - Universidade Fernando Pessoa - Portugal (3)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (12)
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- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (27)
- Bioline International (4)
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- Bucknell University Digital Commons - Pensilvania - USA (1)
- Bulgarian Digital Mathematics Library at IMI-BAS (2)
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- Chinese Academy of Sciences Institutional Repositories Grid Portal (121)
- CORA - Cork Open Research Archive - University College Cork - Ireland (29)
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- DI-fusion - The institutional repository of Université Libre de Bruxelles (1)
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- Duke University (34)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (52)
- Helda - Digital Repository of University of Helsinki (51)
- Indian Institute of Science - Bangalore - Índia (65)
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- INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES (IPEN) - Repositório Digital da Produção Técnico Científica - BibliotecaTerezine Arantes Ferra (1)
- Instituto Nacional de Saúde de Portugal (2)
- Instituto Politécnico de Bragança (1)
- Instituto Politécnico do Porto, Portugal (1)
- Massachusetts Institute of Technology (1)
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- Ministerio de Cultura, Spain (2)
- National Center for Biotechnology Information - NCBI (20)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (6)
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- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (58)
- Queensland University of Technology - ePrints Archive (225)
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- Repositório do Centro Hospitalar de Lisboa Central, EPE - Centro Hospitalar de Lisboa Central, EPE, Portugal (1)
- Repositório Institucional da Universidade de Aveiro - Portugal (2)
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- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (27)
- School of Medicine, Washington University, United States (6)
- Universidad de Alicante (2)
- Universidad del Rosario, Colombia (1)
- Universita di Parma (1)
- Universitat de Girona, Spain (2)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (1)
- Université de Lausanne, Switzerland (6)
- Université de Montréal (1)
- Université de Montréal, Canada (3)
- University of Michigan (1)
- University of Queensland eSpace - Australia (11)
- University of Washington (1)
- WestminsterResearch - UK (2)
Resumo:
Tissue kallikrein, generally existing in living bodies as prokallikrein, is a serine proteinase that has proven of great significance to treat hypertension, cardiopathy and nephropathy. Although the extraction of tissue kallikrein from human urine is the most commonly used method to obtain such a protein, not only the yield is very little, but also the procedure is rather complex. Furthermore, the biological safety is uncertain. Therefore, the preparation of such a protein by genetic engineering method, including gene expression, cell culture, separation and purification, is very important. In this paper, a new method to obtain purified tissue prokallikrein excreted from insect cells by liquid chromatography has been proposed. In contrast to the previously published papers, the purification procedure is simplified to only three steps with the final yield of 57% and the purity of 95%, which is not only convenient, but also low-cost and suitable for the large-scale preparation of such a protein. The purified protein is further validated as prokallikrein by high performance liquid chromatography-mass spectrometry and amino acid sequencing. (c) 2005 Elsevier B.V. All rights reserved.