Mechanisms of H+ modulation of glycinergic response in rat sacral dorsal commissural neurons

Many ionotropic receptors are modulated by extracellular H+. So far, few studies have directly addressed the role of such modulation at synapses. In the present study, we investigated the effects of changes in extracellular pH on glycinergic miniature inhibitory postsynaptic currents (mIPSCs) as well as glycine-evoked currents (I-Gly) in mechanically dissociated spinal neurons with native synaptic boutons preserved. H+ modulated both the mIPSCs and I-Gly, biphasically, although it activated an amiloride-sensitive inward current by itself. Decreasing extracellular pH reversibly inhibited the amplitude of the mIPSCs and I-Gly, while increasing external pH reversibly potentiated these parameters. Blockade of acid-sensing ion channels (ASICs) with amiloride, the selective antagonist of ASICs, or decreasing intracellular pH did not alter the modulatory effect of H+ on either mIPSCs or I-Gly, H+ shifted the EC50 of the glycine concentration-response curve from 49.3 +/- 5.7 muM at external pH 7.4 to 131.5 +/- 8.1 muM at pH 5.5, without altering the Cl- selectivity of the glycine receptor (GlyR), the Hill coefficient and the maximal I-Gly, suggesting a competitive inhibition of I-Gly by H+. Both Zn2+ and H+ inhibited I-Gly. However, H+ induced no further inhibition of I-Gly in the presence of a saturating concentration of Zn2+. In addition, H+ significantly affected the kinetics of glycinergic mIPSCs and I-Gly. It is proposed that H+ and/or Zn2+ compete with glycine binding and inhibit the amplitude of glycinergic mIPSCs and I-Gly. Moreover, binding of H+ induces a global conformational change in GlyR, which closes the GlyR Cl- channel and results in the acceleration of the seeming desensitization of IGly as well as speeding up the decay time constant of glycinergic mIPSCs. However, the deprotonation rate is faster than the unbinding rate of glycine from the GlyR, leading to reactivation of the undesensitized GlyR after washout of agonist and the appearance of a rebound I-Gly. H+ also modulated the glycine cotransmitter, GABA-activated current (I-GABA). Taken together, the results support a 'conformational coupling' model for H+ modulation of the GlyR and suggest that W may act as a novel modulator for inhibitory neurotransmission in the mammalian spinal cord.

结合飞行的快速步行导航技术

在对现有虚拟环境导航技术进行分析的基础上,针对单一导航方式难以同时胜任多种导航任务的问题,提出并实现了一种结合飞行的快速步行导航技术。该导航技术通过对用户交互意图和场景上下文的感知自动调整导航模式以适应不同类型导航任务的需求,从总体上提高了复杂虚拟环境下的导航效率。

黄土高原丘陵沟壑区草地植物群落健康动态评价

植被恢复重建是遏制水土流失的有效措施之一,研究植被恢复重建过程、评价植物群落健康状况对加速植被建设具有重要的实践意义。本文根据黄土丘陵沟壑区的特点,建立了植物群落健康评价指标体系,对草地植被恢复重建过程中的不同阶段的植物群落的健康状况进行了评价。结果表明:植被群落活力变化过程呈抛物线型;群落组织力基本呈波动性变化;恢复力的变化则与活力变化过程相反,在群落活力达到最高水平时,群落的恢复力降至最低;土壤健康呈波动性上升的变化趋势。综合评价表明,植物群落健康水平随着演替过程的发展呈波动性且逐渐上升的变化过程。

中国洪涝灾害之后的生态环境问题警钟急鸣

中国是一个发展中国家 ,正在加速实现工业化、城市化及农业现代化。由于人们的生产活动和经济活动而对生态环境的破坏“助纣为虐” ,才使 1 998年的洪水造成的损失比以往更大 ,并进一步暴露出以城市为中心的环境污染和以农村为纽带的环境退化正破坏和延缓我国国民经济的发展。分析了洪灾之后中国经济发展中环境面临的主要问题和退化症结 ,根据 2 0 1 0年远景目标的要求 ,提出了“提高环保认识 ,开展生态建设 ,控制环境污染”的综合整治对策 ,为中国制定生态环境规划提供基本思路。

我国制造业面临的内外形势及对策研究──抓住机遇,参加全球化敏捷生产体系

：本文从信息时代对人们认识上的影响，讨论了信息时代对生产、对统一的世界市场、对社会的影响。列举了世界经济进入新的繁荣期的特征。讨论了世界生产的形成和发展，市场竞争的三准则，市场竞争的各个历史阶段及全球化敏捷制造体系迅速发展的原因。分析了二十一世纪制造业面临的形势。详细地讨论了我国国有大中型企业的主要问题：1．产品问题；2．企业结构不合理；3．管理问题；4．质量问题；5．工艺手段和装备落后；6．历史包袱沉重等。提出了利用世界全球化生产体系发展的大好形势的三条对策：1．利用敏捷制造原理对企业进行重组；2．加速产品设计队伍的重建，增强新产品的设计能力；3．利用现代化手段实现现代化管理。