Identification of Sarcocystis hominis-like (Protozoa : Sarcocystidae) cyst in water buffalo (Bubalus bubalis) based on 18S rRNA gene sequences

DNA templates were extracted from isolates of Sarcocystis hominis-like cysts collected from cattle and water buffalo, as well as from Sarcocystis fusiformis cysts and Sarcocystis suihominis cysts. The 18S rRNA genes were amplified using DNA from a single

黄牛、水牛体内人肉孢子虫18S rRNA基因研究及虫种鉴定

对自然感染的水牛源的人肉孢子虫以及黄牛源人肉孢子虫DNA的18S rRNA基因的PCR扩增产物进行了测序。对所获的863 bp的18S rRNA基因分析比较表明，二者有较高的同源性，因此认为二者可能同是一种肉孢子虫——人肉孢子虫（Sarcocystis hominis Railliet and Lucet，1891）。由此推断，不仅黄牛可作为人肉孢子虫的中间宿主，水牛也可作为人肉孢子虫的中间宿主。