施氮量对黄土旱塬区冬小麦产量和土壤水分动态的影响

为了探明施氮量对黄土旱塬区冬小麦(Triticum aestivum L.)籽粒产量和麦田土壤水分动态的影响规律,以抗旱性冬小麦品种长武58为供试材料,于2006～2008年连续两个年度在陕西省长武县对不同施氮量条件下麦田土壤贮水量动态、耗水规律、小麦产量和夏闲期降水补给率等特征进行研究。结果表明,麦田土壤贮水量随季节和降水明显变化,同一生育时期2.7m土层的土壤贮水量基本随施氮量的增加而减少。偏旱年每公顷施氮300kg和平水年每公顷施氮225kg均能够获得当年最大的籽粒产量和水分利用效率。每公顷施氮75kg和225kg均能在夏闲期获得较大的降水补给率。每公顷施氮225kg更有利于黄土旱塬区冬小麦的高产和稳产。

施氮和灌水对冬小麦叶片光合特性和籽粒产量的影响

大田条件下设置不同的施氮和灌水水平,研究施氮量和灌水量对冬小麦(Triticum aestivum L.)叶片光合作用生理及籽粒形成的影响。结果表明,施氮和灌水提高叶片Fv/Fm和Pn,并显著提高冬小麦籽粒产量、单位面积穗数和每穗粒数。施氮明显降低千粒重,而适量灌水明显提高千粒重。

小麦/玉米苗期磷累积量对介质供磷水平反应的差异

小麦和玉米苗期是磷素营养的关键期和敏感期,研究两种作物苗期对介质供磷反应,可为合理施用磷肥提供参考。试验设缺磷对照、低磷胁迫、中等磷胁迫和正常供磷(P_2O_5含量分别为0、0.05mmol·L~(-1)、0.3mmol·L~(-1)和0.5mmol·L~(-1))4种磷水平,选取小麦"小偃22号"、"兰考4号"和玉米"屯玉65号"、"户单4号"为指标作物,用营养液培养法研究小麦、玉米苗期磷累积量对介质不同供磷水平的反应差异。结果表明,不同介质供磷水平下,两种作物苗期磷累积量显著不同且因作物类型、基因型、器官及测定时期不同而异。总体而言,介质供磷后,苗期早期生长阶段(出苗后25d以前),小麦的介质最佳供磷水平较玉米高;苗期后期(出苗后40～50d),小麦和玉米最佳供磷水平一致。如果以低磷胁迫作为对比进行分析,玉米苗期整株磷累积量对介质供磷的敏感性比小麦强;从不同基因型来看:"兰考4号"对介质供磷的敏感性强于"小偃22号","屯玉65号"和"户单4号"基本一致。缺磷条件下小麦较玉米磷效率高,供磷条件下玉米较小麦高;但不同基因型间规律性较差。

干旱对不同冬小麦旗叶光合产物供应能力的影响

研究干旱对小麦旗叶光合产物供应能力的影响,揭示小麦抗旱高产的生理机制,为提高小麦的抗旱能力及高产稳产提供理论依据。【方法】在防雨池栽培条件下,以旱地冬小麦品种长武134(抗旱性强)和水地冬小麦品种陕253(抗旱性弱)为试材,以适宜水分处理为对照(CK,土壤含水量为田间持水量的70%～75%),研究干旱处理(土壤含水量为田间持水量的50%～55%)对不同冬小麦旗叶光合产物供应速率(净光合速率和蔗糖合成能力)和供应持续期的影响。【结果】与对照相比,干旱处理降低了冬小麦灌浆中后期旗叶净光合速率,缩短了净光合速率高值持续期(PAD),其中长武134降幅较小,净光合速率较高;干旱处理提高了冬小麦灌浆初期旗叶的蔗糖磷酸合成酶(SPS)活性,其中长武134增幅较大,且在灌浆中后期依然能保持相对较高的蔗糖供应能力;干旱处理缩短了冬小麦叶绿素含量缓降期(RSP),提高了丙二醛(MDA)含量,加速了旗叶的衰老,缩短了光合产物的供应持续期,其中长武134受干旱影响较小;干旱处理降低了冬小麦灌浆中后期主茎穗粒质量积累量及其速率,其中长武134降幅较小。【结论】干旱条件下,抗旱品种长武134旗叶在灌浆中后期可维持较高的光合产物...

缓释尿素对棕壤农田线虫群落的影响

通过田间随机区组试验，就缓释尿素对棕壤农田线虫群落产生的影响进行了研究。本试验设置4个处理，（1）普通尿素（U）；（2）U＋脲酶抑制剂LNS（SRU1）；（3）SRU1＋硝化抑制剂双氰胺（DCD）（SRU2）；（4）SRU1＋硝化抑制剂3, 5-二甲基吡唑（DMP）（SRU3）。结果表明，在整个春小麦（Triticum aestivum L.）生育期内，SRU1、SRU2和SRU3处理的土壤脲酶活性低于U处理，且SRU2、SRU3 处理的土壤NH4+-N含量在较长时间内维持在较高水平；4个处理共鉴定土壤线虫39个属，其中Cephalobus和Aphelenchus为优势属，所有处理均以食细菌线虫为优势营养类群；小麦抽穗期、成熟期，SRU3处理土壤线虫总数、食细菌线虫数量以及小麦成熟期SRU3处理的土壤捕食/杂食线虫数量显著高于U、SRU1和SRU2处理（p < 0.05）；香农多样性指数（H′）、优势度指数（λ）表明小麦成熟期SRU3处理的物种多样性较高，且线虫群落组成较为均衡；脲酶抑制剂和硝化抑制剂组合型缓释尿素能够对土壤线虫群落结构产生有益的影响。

活性X-3B红染料污染生态毒理效应及机量研究

染料是一大类合成化学品，在纺织、印刷、信息、军事工业、医学及其他多个领域都得到了广泛的应用。然而，随着染料的不断生产和广泛使用，由它引发的土壤一作物系统染料污染已成为当前人们所关注的重要环境问题之一，有关染料污染的生态毒理效应及其机理的研究已成为污染生态化学研究的前沿领域和重要问题。本论文根据我国当前有机染料污染的趋势和有关研究现状，针对迫切需要对染料污染生态毒理效应进行了解的现实情况，以活性X-3B红为例，探讨了活性X-3B红在单一污染条件下对小麦（Triticum aestivum）、白菜（Brassicachinensis）和水稻（Oryza sativa )等常见作物的种子发芽与根伸长的生态毒性效应及其机理，以及我国三种典型土壤（红壤、褐土和棕壤）中脉酶和脱氢酶对活性X-3B红的耐受性及其机理；在种子和幼苗暴露的单一污染试验的基础上，又进一步地研究了活性X-3B红分别与无机污染物（重金属Cd）和有机污染物（农药甲胺磷）复合污染条件下对小麦、白菜种子发芽、根伸长及幼苗生长的毒性效应。实验结果表明，活性X-3B红染料单一污染条件下对三种作物产生不同的生态毒理效应，三种土壤的脉酶和脱氢酶对其都表现出了一定的耐受性，但耐受能力各异；在活性X一3B红与Cd复合污染的实验中，小麦根伸长对其联合作用较种子萌发更为敏感，在Cd不同的浓度范围内表现出协同或是拮抗复杂的效应；在活性X-3B红与甲胺磷复合污染的条件下，小麦和白菜根伸长和生物量的反应有差异，但除小麦根伸长有协同和拮抗两种效应外，总的来看都表现出微弱或明显的拮抗效应。这些研究，有望促进人们对染料污染生态化学行为进行了解，为合成低生态风险的有机染料提供具有参考价值的基础资料与科学依据。

不同小麦品质类型与生态环境关系的研究

小麦（Triticum aestivum L.）是世界上种植面积最大，总产量最高，食物加工种类最丰富的粮食作物，占世界人口35 ％－40 ％的人们以此为主要食物。因此小麦产量的高低和品质的优劣直接影响人们对食物需求的安全和满意程度，也影响着人类的营养平衡以及面粉和食品加工业的发展。随着生活水平的提高，人们对于小麦的品质越来越重视。培育优质专用小麦新品种，制定优质专用小麦品种品质生态区划，从而在不同程度上实现小麦的区域化种植和产业化经营具有重要的意义。 影响小麦品质的因素主要是遗传因素和环境因素，其中环境因素又包括各种自然生态因素和人为因素。研究表明，小麦品质的环境间的差异大于品种间的差异，气候条件是影响小麦品质的最重要的因子，小麦品质的地域间的差异反映出了小麦的品质区域分布规律。为了满足市场对不同品质小麦的需求，对小麦进行区域化研究具有重要的理论和现实意义。本研究结合四川的地理、气候特点，研究不同品质类型与生态环境的关系，为在复杂的生态环境内进行品质区划提供依据。 本研究首先根据四川省小麦种植区域的生态特点，在四川省多个典型生态区：川南丘陵的荣县、川西南高原的西昌、川西平原的双流布点种植，采用的小麦试验材料为不同品质类型：中筋小麦川育12、川育14、川育16由本所提供；弱筋小麦川麦32和强筋小麦川麦36由四川省农科院作物所提供。通过研究品质性状与品种及各个生态因子包括地点、土壤土质差异等的关系，明确不同生态环境中适宜种植的小麦品种类型，强筋小麦、中筋小麦更适合于在荣县、双流地区种植，弱筋小麦更适合于在西昌地区种植，为品种品质区划奠定基础。 其次，选择了本课题组育成的稳定中间品系，对其品质性状SDS沉降值进行了多年测定。分析了品质性状SDS沉降值与多种气候因子的相关性，结果表明SDS沉降值与日均温、日照时数成正相关，与降水量成负相关，为品质育种提供了理论依据。 此外，以中筋小麦新品种小麦川育14为材料，应用三元二次正交旋转回归模型设计试验，研究主要栽培因子播期、密度和施肥量对产量的影响，并建立函数模型。经计算机模拟寻优，筛选出了高产高效栽培组合措施，并确定了置信域。结合四川省不同的地理情况，在平原和丘陵地区分别进行实验，并各自建立了高产高效栽培组合措施，为川育14品种的推广提供了理论指导。 Wheat is one of the most important crops in the world. About 35%-40% people all over the world, take the wheat as their most important food. So the quality, as well as the quantity of the wheat makes a direct effect on people’s demands of food and their satisfaction. It also effects on human’s healthy, and the development of the Food processing industry. With the development of the living standard, people pay more attention to the quality of wheat. So, we set a special ecology zoning for wheat. It is significant to carry out planting the wheat in special zoning in varying degrees. The main factors affecting wheat quality are heredity and environment including many ecological factors and the factors in cultivation. As to the quality，the difference between ecology and cultivation is more important than the difference between special wheat. In so many factors, climate is the most important one. From the difference in quality between different zones，we can conclude the rule of distribution abort quality of wheat. Finding out the intersection of numerous wheat not only can meet the demand of food production，but also has important signification in theory and realism。In our research, according to the complex geography in Sichuan province, we study the relationship between numerous kinds of quality characters in wheat and the ecology. So, we can set a foundation for more research. In this research, firstly, we plant wheat in some typical ecological regions of SICHUAN province: RONGXIAN（south of SC）、XICHANG（south of SC）, SHUANGLIU(west of SC). The materials of the experiments: ChuanYu12, ChuanYu14, ChuanYu16(from our institute), Chuanmai32, Chuanmai36 (from the Chinese academy of agriculture sciences of Sichuan. Through the research on the relationship between the quality of wheat and those ecology factors, we can make a definition that which area is perfect matched with which kind of wheat. And it can satisfy the demand of people. Secondly, select many sorts of wheat from our research group. All of them are selected and bred more than 3 years(2003-2005). And we make every-year determination as well. We’ve gotten SDS value from those 9, and various data on factors of climate. We also got to know the relation ship between those numbers. Thirdly, use Chuanyu14 as material, the mathematical model of the relation between the production of wheat and main agricultural measures such as date, density and fertilizer. The model was established by association of three elements two return, rotate and regression. We set a suitable model and get a suitable method which can make high harvest. Based on various kinds of geographical regions in Sichuan province, we set different models which can be used in plain and hill. So, we can plant Chuan Yu 14 in Sichuan province under the result in research.

来自易变山羊草抗禾谷孢囊线虫基因的克隆与序列分析

禾谷孢囊线虫（Heterodera avenae）是严重危害禾谷类作物的病原线虫之一,它广泛分布于澳大利亚、欧洲、北美、印度和中国等世界主要小麦产区,使作物严重减产,造成巨大的经济损失。目前最有效的防治措施之一是将外源抗性基因导入栽培小麦(Triticum aestivum L.)，培育抗禾谷孢囊线虫的新品种。但迄今为止抗禾谷孢囊线虫基因克隆研究的相关报道却很少。 本实验根据此前从抗禾谷孢囊线虫材料E-10扩增得到的与来自节节麦（Aegilops tauschii）的抗禾谷孢囊线虫基因Cre3高度同源的序列Rccn4,设计出三条嵌套引物,采用SON-PCR(single oligonucleotide nested PCR)方法,从E-10基因组DNA中得到一个长为1264 bp的扩增产物(命名为Rccn-L),测序比对结果显示,这一序列将Rccn4的3’端延伸了1209 bp,与抗禾谷孢囊线虫Cre3基因核苷酸同源性为86﹪，核苷酸编码区长1026 bp，含一个不完整的开放阅读框,一个终止密码子，没有起始密码子和内含子结构,编码一个342个氨基酸残基的蛋白质。该蛋白质等电点为5.19，分子量为38112.6Da。从序列的第113位开始到第332位是NBS-LRR类抗病性基因LRR区,呈现XXLXXLXXL重复。LRR编码区内亮氨酸残基的含量达17﹪，与抗禾谷孢囊线虫Cre3基因LRR编码区的核苷酸和氨基酸同源性分别为89﹪和78﹪。本实验首次将SON-PCR成功地运用于植物基因克隆，为植物基因克隆提供了又一有效方法。 此外,还根据Cre3基因及其他的NBS-LRR类植物抗性基因的NBS和LRR区保守序列设计了两对特异性引物，从禾谷孢囊线虫抗性材料易变山羊草基因组DNA中扩增到两个相应的目标条带。测序分析结果表明，它们的长度分别为532bp和1175bp，构成了一个有32bp的共同序列的NBS-LRR编码区。其序列总长为1675bp（命名为RCCN），含有一个不完整的开放阅读框，没有起始密码子、终止密码子和内含子结构。其中编码序列为1673bp，可编码一个557个氨基酸的蛋白质，等电点（pI）为5.39，分子量为63537.5Da。与Cre3的核苷酸和氨基酸同源性分别为87.8﹪和77﹪。RCCN氨基酸序列中含有已知抗病基因NBS区域的几个保守模体：kinase2区的ILDD、kinase3的（ⅰ）ESKILVTTRSK，（ⅱ）KGSPLAARTVGG，（ⅲ）RRCFAYCS及EGF。RCCN NBS区与Cre3 NBS区的核苷酸和氨基酸的同源性分别为96.4﹪和94﹪。从氨基酸序列的274位到548位为LRR保守区，呈现不规则的aXXLXXLXXL（其中a代表I，V，L，F或M）重复，其中亮氨酸的含量为15.6﹪。该区域与Cre3的LRR区的核苷酸和氨基酸同源性分别为80.8﹪和74﹪。推测该序列可能为一个抗禾谷孢囊线虫的新基因。 本文对抗禾谷孢囊线虫基因的克隆研究，为进一步克隆基因全序列，探索其结构与功能，和研究该基因表达与调控提供了关键信息。同时也为通过基因工程途径将抗性基因向优良小麦品种高效、定向转移，最终培育出小麦抗禾谷孢囊线虫新品种奠定了基础。 Cereal cyst nematode (CCN) is a damaging pathogen of broad acre cereal crops in Australia, Europe, North America, India and China. It affects wheat, barley, oat and triticale and causes yield loss of up to 80%. At present, Transferring resistance genes against CCN into wheat cultivars and breeding varieties are considered one of the most effective methods for controlling the CCN. However, there are very limited reports concerning the cloning studies of resistance genes against the cereal cyst nematode. According to the sequence of Rccn4 which had high similarity to the nucleotide binding site (NBS) coding region of cereal cyst nematode resistance gene, Cre3， We designed three 3’ nested primers. Using single oligonucleotide nested PCR (SON-PCR) we successfully amplified one band, Rccn-L, of 1264bp from E-10 which is the wheat-Ae.variabilis translocation line containing the cereal cyst nematode resistance gene of Ae.variabilis. We found that this band of interesting is the 3’ flanking sequence of 1209bp in size of Rccn4. The coding region was 1026bp, which contained an incomplete open reading frame and a terminator codon, without initiation codon and intron, encoding a peptide of 342 amino acid residues, and shared 86﹪nucleotide sequence identity with Cre3. This peptide had a conserved LRR domain, containing the imperfect repeats,XXLXXLXXL, which contains 17﹪ leucine residues and shares, respectively, 89﹪ nucleotide sequence and 78﹪ amino acid sequence identity with the LRR sequence of Cre3 locus. This research firstly used SON-PCR in the research of plant genome successfully, which indicated that SON-PCR is another method of cloning plant gene. At the same time, According to the conversed motif of NBS and LRR region of cereal cyst nematode resistance gene Cre3 from wild wheat (Triticum tauschlii L.) and the known NBS-LRR group resistance genes, we designed two pairs of specific primers for NBS and LRR region respectively. One band of approximately 530bp was amplified using the specific primers for conversed NBS region and one band of approximately 1200bp was amplified with the specific primers for conversed LRR region. After sequencing, we found that these two sequences included 32bp common nucleotide sequence and have 1675 bp in total, which was registered as RCCN in the Genbank. RCCN contained a NBS-LRR domain and an incomplete open reading frame without initiation codon, terminator codon and inxon. Its exon encodes a peptide of 557 amino acid residues. The molecular weight of the protein from the amino acid was 63.537 KDa. The amino acid sequence of RCCN contained conserved motif: ILDD, ESKILVTTRSK, KGSPLAARTVGG, RRCFAYCS, EGF，LRR. RCCN shares 87.8﹪ nucleotide sequence and 77﹪ amino acid sequence identity with cereal cyst nematode gene Cre3. It might be a novel cereal cyst nematode resistance gene. These research results of cloning the resistance genes against cereal cyst nematode bring a great promise for transferring resistance genes into wheat cultivars and breeding new wheat varieties against cereal cyst nematode by gene engineering. And these results also lay the hard foundation for the expressing researches of these genes.

在盐胁迫下外源维生素浸种对小麦发芽及幼苗生长的影响

维生素(Vitamin)又称维他命，为“万年青”产品，是维持人体生命健康必需的一类低分子有机化合物质。维生素对人体健康的作用人们研究很多, 维生素可以增强人体对感染的抵抗力，降低出生缺陷及降低癌症和心脏病发病率等，一旦缺乏，肌体代谢就会失去平衡，免疫力下降，各种疾病，病毒就会趁虚而入；而维生素对作物影响的研究却很少。目前为止，尚无对用维生素浸种的方法来研究外源维生素是否对小麦种子萌发及幼苗生长起调节作用的报道，且对其在小麦抗逆性方面影响的研究甚少，对盐的胁迫抗性研究尚未有人报道。小麦（Triticum aestivum L.）属于拒盐的淡土性作物。盐害不利于小麦生长，严重影响小麦的产量和品质。本研究采用4 种不同维生素VB1、VC、VB6、VPP，分别对供试小麦品种川育12（红皮）、川育16（白皮）小麦浸种后，在一般自然条件下和逆境（盐胁迫条件）下，进行试验。探讨在正常情况下与在不同盐浓度条件下，各维生素及盐浓度对小麦发芽及幼苗生长的影响，并且比较两种不同皮色的小麦在相同盐胁迫条件下的差异表现，同时研究维生素处理的特异性，且哪种维生素对盐害缓解作用最佳。研究结果表明：在无盐胁迫（自然）条件下，对用4 种不同维生素VB1、VC、VB6、VPP 浸种小麦川育12、川育16 后的种子萌发及幼苗生长（幼苗的根长、根重、苗高、苗鲜重）的研究结果表明：4 种外源维生素浸种均对小麦发芽有调节作用，都能提高其最终发芽率。但是提高幅度有所差异。用VB6 浸种后的小麦提高幅度最多，VC 次之，VPP 提高幅度最小。同时，4 种外源维生素浸种对小麦种子的出芽速度及芽后长势也有一定的影响。VB6、VC 处理的小麦种子出芽速度最快，萌发后长势最好；VB1 出芽速度相对较慢，VPP 最慢,但都大于对照；VB1 处理长势略高于对照，VPP 处理的小麦长势则低于对照。从整体来看，VB6、VC处理促进效应明显， VB1 次之，而VPP 在某些方面无效甚至产生负效应。此外，相同的维生素处理对不同的品种的种子萌发、生长效果也存在差异，各种维生素作用于川育12 的效应均强于对川育16。进一步对幼苗根系TTC 还原力及幼苗叶片中硝酸还原酶活性进行测定、分析。研究发现：并非所有种类的维生素对幼苗根系TTC 还原力及幼苗叶片中硝酸还原酶活性的提高都有帮助。幼苗根系TTC 还原力在不同维生素处理下存在显著差异，而与小麦品种关系甚微。经VB6、VC 处理后，根系TTC 还原力测定值均显著高于对照，VB1 不明显，VPP 则略低于对照。VB6、VC 处理的幼苗叶片中硝酸还原酶的含量大于对照，VB1 与对照相差无几，而VPP 处理的川育12 幼苗叶片中的硝酸还原酶活性比对照CK 略高，而在川育16 中则略比对照CK 有所下降，呈现出抑制效应。综上结果表明：VB6、VC 具有促进种子发芽，幼苗生长及根系生长的作用，是较好的促生长剂；VPP 具有抑制作用，是较好的抑制剂，可进一步研究、开发利用。在盐胁迫条件下，对用4 种不同维生素VB1、VC、VB6、VPP 浸种川育12、川育16 后的种子萌发及幼苗生长（幼苗的根长、根重、苗高、苗鲜重）的研究结果表明：在不同盐浓度胁迫条件下， 各处理的种子萌发及幼苗生长均受到不同程度的抑制。随着盐浓度的增加, 发芽率、发芽指数和活力指数成下降趋势；幼苗的根长、根重、苗高、苗鲜重不断降低。4 种维生素处理间也表现出较大差异。VB6、VC 在每个处理中均保持对盐害的缓解作用，VB6 较VC 更易于促进发芽及幼苗生长。最终发芽率高，根系多、长、重，苗高高、重。而VB1、VPP 则表现出抑制作用。在高盐浓度150mM 时，4 种维生素浸种后的种子，其最终发芽率均不能达到40％，但VB6、VC 处理最终发芽率、苗重、根重均高于对照，VPP 最终发芽率、苗重、根重均低于对照。进一步对幼苗根系TTC 还原力及幼苗叶片中脯氨酸含量进行测定、分析。研究发现：不同盐浓度，不同维生素处理、不同品种间存在差异。随着盐浓度的增加（75mM，100mM，150mM），幼苗根系TTC 还原力活性成下降趋势，幼苗叶片中脯氨酸的积累量成上升趋势。VB6 处理脯氨酸含量增加最为明显，VC 次之，VPP 与对照接近，其变化幅度最小。经VB6、VC 处理后的幼苗根系还原强度，在不同盐浓度下，测定值均显著高于对照，VB1 不明显，VPP 则低于对照，产生负效应。此外，品种间表现不尽相同，相同的维生素处理，相同的盐浓度对不同的品种的种子萌发、生长效果也存在差异， 4 种维生素对川育16 的作用均强于川育12，但其影响趋势是一致的。说明VB6、VC 具有耐（抗）盐性，可以促进种子发芽和幼苗生长，是较好的耐（抗）盐拌种剂。 Vitamin is one kind of necessary low molecular compound for humans tosustain health and life. Lots of Studies have been done on the effectc of the vitaminsfor people. Vitamin can help people improve the body's natural resistance to disease,Drop the rate of birth defects、cacers and the incidence of the heart diseases. Ifpeople have less of them, the metabolism of the organism may throw off balance,immunity may drop off, and catch disease; Though the effects for Vitamin to thecrops are limited. up to now, there’s no one use soking seeds of wheats with vitaminsas a method, to study on how the effects will happen on the wheat seed germinationand seedling growth, and there are only few reserches on antireversion force forwheats ,none for the antireversion force in Sault stress condition.Wheat（Triticum aestivum L.）is sensitive to the salt, so the salt damage will doharm to wheat’s growth, it will have an unfavorable impact on the output and thequality of wheat.On this reaserch, we Soaking CHY12(red)、CHY16 (white) wheat seeds withVitamin C, B1, PP, B6 (50mg/L) as a pretreatment first. Then under two condition: one is in the normal environment the other is in different Salinity, we begin ourexperiments. Then disscuss on if the vitamin and salinity affect the wheat seedgermination and seedling growth, and what is the different between the two of them,the result shows that:Under the normal condition, after soaking seeds with VB1、VC、VB6、Vpp，we study on the their seed germination and the seeding growth(the root length andweights, The seedling heights and weights), it shows that all of those four kinds ofvitamin can adjust the seed germination, but different in The growth rate. VB6 isbest for increase, VC comes second,VPP is the worst. Meanwhile, those four vitaminalso have effect on the speed of the sprouting of the wheat. VB6、Vc can faster theseed germination most, and the seedlings are all doing well; VB1 do little effects onthe budding, Vpp is the worst, but all treatments are better than CK; but in Vi, VB1some what above the CK, while VPP lower than that. On the whole, the acceleratingeffect of VB6、VC are obvious, VB1 takes second place, but VPP in some aspects arenoneffective even have negative effect. Furthermore, different kind of seeds with thesame vitamin may different in seed germination and seedling growth, four vitaminson CHY16 is better than CHY12.More studies on TTC reductive capacity of roots and the activity of nitratereductase in the leaves, the reasult shows not all the vitamin can help the seedlings toimprove the TTC reductive capacity and the activity of nitrate reductase. TTCreductive capacity in different treatments shows significant differences,but notcorrelate to the variety of the wheat. The TTC reductive capacity of VB6、Vctreatments are all higher than CK, VB1 is nearly the same as CK, VPP is a littlelower than CK. Through the study of acivity of nitrate reductase, it shows that,VB6、VC are higher than CK ,VB1 is nearly the same as CK also, VPP is a little higher inthe CK of CHY12 but lower in CHY16. Through all the results above: VB6、Vc helpthe wheat seed germination, seedling growth and the growth of roots, is theperfectable factor of stimulating the growth; Vpp is a inhibition, that’ll be furtherreserch,and well develop and utilize in the future.Under the different Salinity condition, after soaking seeds with VB1、VC、VB6、Vpp，we study on the their seed germination and the seeding growth(the root lengthand weights, The seedling heights and weights), it shows that: under differentsalinity, the seed germination and the seedling growth of any treatment are inhibited.With the increase of the concentration, the germination rate, Vi、Gi all had fallen; theroot length and weight, the seedling heights and weights steadily sank down. There are also have pronounced difference between all treatments with four differentvitamins.VB6、VC in all treatments are alleviative the salt damage, VB6 is easier tocause to put forth buds than VC, and it’s quantitative value is the highest in theultimate germination rate, in root and seedlings’ hight and weight. Though the VPP、VB1 are seems to inhibite its growth. Under the high concentration150mM Nacl, theultimate germination rate in all treatments are below the 40%, but VB6、VC’squantitative values in any experiments are higher than CK,while VPP lower thanCK.Then we study on the TTC reductive capacity of roots and the content of Polinein leaves, the result shows that between the different salinity, different vitamintreatments, different varieties of the wheat have discrepancy.along with theincreasing concentraion of the salinity（75mM，100mM，150mM），TTC reductivecapacity of roots decreases, the accumulation of the content of Poline in leaves havean upward trend. The increase of VB6’s treatment are obviously, VC comessecond,VPP is nearly come up with CK, changes a little. In TTC reductive capacity of roots’s reserch, VB6、VC are higher than CK at any time,VB1 is not palpable,VPP is lower than CK, makes negative affect on wheat. In addition, varieties of thewheats are remain different, no matter it shows promoting or inhibiting, all fourvitamins have moreobvious effects on CHY16 than CHY12, but the tendency of theeffection are the same. It is say that VB6、VC can help wheat to standwith the saultwell, and promot in growth，they are the better reagent to mix with the seed.

CO_2和O_3浓度升高对春小麦活性氧代谢及抗氧化酶活性的影响

【目的】揭示CO2和O3浓度升高及其复合作用对植物活性氧(ROS)代谢及抗氧化酶活性的影响机理。【方法】以春小麦(Triticum aestivum L.)为试材,利用开顶式气室(OTCs)研究CO2和O3浓度升高及其复合作用下,春小麦叶片膜脂过氧化程度,活性氧产生速率、含量及抗氧化酶活性的变化。【结果】在整个生育期内,与对照相比,高浓度CO2[(550±20)μmol·mol-1]处理下,春小麦叶片相对电导率、MDA含量减小,产生速率、H2O2含量下降,SOD、CAT、POD和APX活性增强;而在O3浓度为(80±10)nmol·mol-1的条件下,春小麦叶片相对电导率、MDA含量增大,产生速率、H2O2含量升高,SOD、CAT、POD和APX活性总体上有所减弱;CO2和O3浓度升高复合[(550±20)μmol·mol-1+(80±10)nmol·mol-1]处理下,春小麦叶片MDA含量、产生速率和SOD活性总体上低于对照,而相对电导率、H2O2含量以及CAT、POD和APX活性总体上增加。【结论】CO2浓度升高抑制了春小麦叶片活性氧的代谢速率,提高了抗氧化酶的活性,对春小麦表现为保护效应,而O3浓度升高促进了春小麦叶片活性氧的代谢速率,降低了抗氧化酶的活性,对春小麦表现为伤害效应。在CO2和O3浓度升高复合处理下,CO2浓度升高在一定程度上缓解了O3浓度升高对春小麦的伤害效应,而O3浓度升高亦在一定程度上削弱了CO2浓度升高对春小麦的保护效应。

水肥空间组合对成熟期冬小麦各器官氮磷养分分配的影响

以肥熟土垫旱耕人为土为供试土壤,冬小麦(Triticum aestivum L.)小偃22为供试植物,在全生育期人工控制土壤水分条件下,采用分层隔水土柱法研究了与田间土层分布相同土柱不同土层水分、氮、磷组合对冬小麦不同器官氮、磷养分累积及分配的影响.结果表明:(1)冬小麦不同器官氮、磷累积量表现为籽粒茎秆、叶>穗余部>根系.(2)与整体湿润处理相比,上干下湿水分处理可降低小麦各器官氮、磷累积量,但仅籽粒氮、磷累积量所占比例减少,而营养器官氮累积量所占比例均增加.(3)从肥料处理看,单施氮、单施磷和氮磷配施处理的小麦植株各器官氮、磷累积量均比对照增加,但籽粒氮、磷累积量所占比例均减小.(4)施肥层根系氮、磷累积量比对照相对增加,并以0～30cm土层根系氮、磷累积量为最高.(5)单施氮条件下,以0～90cm土层施肥各器官氮、磷累积量最高,0～30cm土层施肥最低;单施磷和氮磷配施时,以0～90cm土层施肥处理籽粒氮、磷累积量最高,其次是0～30cm土层施肥.由于石灰性土壤中肥料氮终产物以硝态氮为主且容易移动,而磷肥不易在土壤中迁移,在生产实践中仍以氮磷配施入0～30cm土层为佳.

不同类型作物对干湿交替环境的反应

通过对干湿交替环境下春小麦、马铃薯、大豆和玉米等作物的产量、水分利用效率及光合作用、蒸腾作用、气孔导度等生理变化的研究表明 :( 1 )春小麦和马铃薯在干湿交替环境下可获得与充分供水相当的产量而它们的水分利用效率却显著提高 ,大豆减产幅度较大 ,玉米减产严重 ,其水分利用效率显著低于全湿处理 ;( 2 )浇水后各作物的光合速率、蒸腾速率和气孔导度都有所增加 ,但不同作物增加的幅度不同 ,就是同一作物各指标的增幅也不同 ;( 3)干湿交替环境下同化物的运输模式有利于春小麦籽粒的充实和马铃薯块茎的膨大 ,而不利于玉米产量的形成 ;( 4 )产量不仅决定于营养生长阶段 ,更主要决定于生殖生长阶段。此外 ,还就干湿交替过程中若干生理变化和经济产量形成机制作了初步探讨。

水肥空间组合对冬小麦生物学性状及生物量的影响

目的】研究水肥空间组合对冬小麦形态指标及生物量的影响,对指导旱地施肥具有一定理论和实践意义。【方法】以肥熟土垫旱耕人为土为供试土壤,在全生育期遮雨和人工控制土壤水分条件下,采用分层隔水土柱试验法研究与田间土层分布相同土柱不同土层水分、氮、磷组合对冬小麦叶面积、株高、分蘖数、生物量、根冠比和收获指数等指标的影响。【结果】与整体湿润水分处理相比,上干下湿水分处理(0～30cm土层干旱胁迫,30～90cm土层湿润)下,抽穗期小麦旗叶面积、株高分别降低7.03%和3.77%;小麦地上部和根系生物量及收获指数也不同程度降低,但根冠比增加。从肥料处理看,单施磷和氮磷配施处理,小麦叶面积、株高、有效分蘖数和总生物量均极显著高于单施氮和CK,这与供试土壤各土层严重缺磷,而氮素供应相对丰富有关。从不同土层施肥看,在两种水分处理下,单施氮时,以均匀施入0～90cm土层小麦叶面积、株高、有效分蘖数、地上部生物量和根系生物量最高,施入0～30cm土层最低;单施磷和氮磷配施时,0～90cm与0～30cm土层施肥间总叶面积、旗叶面积、株高、有效分蘖数以及总生物量差异不显著,但均显著高于30～60cm和60～90cm土层相应施肥处理。【...

苜蓿干草和秸秆组合体外发酵营养特性及其利用研究

应用体外发酵产气技术,评价了苜蓿Medicago sativa干草和玉米Zea mays、小麦Triticum aestivum秸秆分别以0∶100、25∶75、50∶50、75∶25和100∶0进行两两组合时的发酵特性。结果表明：不同比例组合产气量（GP）、理论最大产气量（A）、产气速率（b）及产气延滞时间（LAG）变化趋势不同;苜蓿干草与玉米秸秆按50∶50的比例或苜蓿干草与小麦秸秆按75∶25的比例组合时的效应明显好于其他组合。48 h产气量与粗蛋白（CP）（P〈0.05）及中性洗涤可溶物（NDS）含量呈正相关关系,而与中性洗涤纤维（NDF）、酸性洗涤纤维（ADF）、半纤维（HC）含量及NDS/CP（P〈0.01）呈负相关关系;理论最大产气量与CP、NDS的含量呈正相关关系,与NDF、ADF、HC和NDS/CP（P〈0.01）呈负相关关系;产气速率与CP（P〈0.01）、HC（P〈0.01）、NDS（P〈0.01）呈极显著正相关关系,分别与NDF（P〈0.01）、ADF（P〈0.01）、NDS/CP（P〈0.05）呈负相关关系;产气延滞时间与饲草料的主要营养成分的相关关系不明显,只与NDS/CP（P〈0.05）呈显著正相关关系。结论认为,饲草中非结构性碳水化合物与蛋白质比例决定了体外发酵产气的特性。生产实践中应针对低质粗饲料营养特性,适当添补易发酵或高蛋白牧草,提高粗饲料利用效率。

Cytological and Molecular Identification of Alien Chromatin in Giant Spike Wheat Germplasm

Alien chromosomes of twelve giant spike wheat germplasm lines were identified by C-banding, genomic in situ hybridization (GISH), sequence characterized amplified region (SCAR), and random amplified polymorphic DNA (RAPD). All lines showed a chromosome number of 2n = 42, five of them carried both a pair of wheat-rye (Triticum aestivum-Secale cereal) 1BL/1RS translocation chromosomes and a pair of Agropyron intermedium (Ai) chromosomes, three carried a pair of Ai chromosomes only, three others carried a pair of 1BL/1RS chromosomes only, and one carried neither 1BL/1BS nor Ai chromosome. Further identification revealed that the identical Ai chromosome in these germplasm lines substituted the chromosome 2D of common wheat (Triticum aestivum L.), designated as 2Ai. The genetic implication and further utilization of 2Ai in wheat improvement were also discussed.