Identification of larval sea basses (Centropristis spp.) using ribosomal DNA-specific molecular assays

The identification of sea bass (Centropristis) larvae to species is difficult because of similar morphological characters, spawning times, and overlapping species ranges. Black sea bass (Centropristis striata) is an important fishery species and is currently considered to be overfished south of Cape Hatteras, North Carolina. We describe methods for identifying three species of sea bass larvae using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) assays based on species-specific amplification of rDNA internal transcribed spacer regions. The assays were tested against DNA of ten other co-occurring reef fish species to ensure the assay's specificity. Centropristis larvae were collected on three cruises during cross-shelf transects and were used to validate the assays. Seventy-six Centropristis larva were assayed and 69 (91%) were identified successfully. DNA was not amplified from 5% of the larvae and identification was inconclusive for 3% of the larvae. Those assays can be used to identify sea bass eggs and larvae and will help to assess spawning locations, spawning times, and larval dispersal.

Die Anwendung molekulargenetischer Verfahren in der fischereiwissenschaftlichen Forschung - Trennung von Fischpopulationen

The discrimination of stocks and separate reproductive units within fish species to facilitate fisheries management based on biological data has always been a challenge to fisheries biologists. We describe the use of three different molecular genetic techniques to detect genetic differences between stocks and closely related species. Direct sequencing of the mitochondrial ND3 gene describes the relationship between different aquaculture strains and natural populations of rainbow trout and revealed genetic homogeneity within the hatchery strains. Microsatellite analyses were used to explore the differences between redfish species from the genus Sebastes and to verify populations structure within S. mentella and S. marinus. This lead to an un equivocal discrimination of the species and an indication of populations structure within those species in the North Atlantic. The Amplified Fragment Length Polymorphisum (AFLP) methodology revealed genetic differences between Baltic and North Sea dap (Limanda limanda)and a possible population structure within the North Sea.

Genetic and morphological differences between Sebastes vulpes and S. zonatus (Teleostei: Scorpaeniformes: Scorpaenidae)

The taxonomic status of Sebastes vulpes and S. zonatus were clarified by comprehensive genetic (amplif ied fragment length polymorphisms [AFLP] and mitochondrial DNA [mtDNA] variation) and morphological analyses on a total of 65 specimens collected from a single locality. A principal coordinate analysis based on 364 AFLP loci separated the specimens completely into two genetically distinct groups that corresponded to S. vulpes and S. zonatus according to body coloration and that indicated that they are reproductively isolated species. Significant morphological differences were also evident between the two groups; 1) separation by principal component analysis based on 31 measurements, and 2)separation according to differences in counts of gill rakers and dorsal-fin spines without basal scales, and in the frequencies of specimens with small scales on the lower jaw. Restriction of gene flow between the two groups was also indicated by the pairwise ΦST values estimated from variations in partial sequences from the mtDNA control region, although the minimum spanning network did not result in separation into distinct clades. The latter was likely due to incomplete lineage sorting between S. vulpes and S. zonatus owing to their recent speciation.

Genetic diversity of Bartail flathead (Platycephalus indicus (Linnaeus,1758)) using AFLP markers in Persian Gulf

Platycephalus indicus is a large benthic fish that inhabits temperate and tropical coastal waters of the Indo-West Pacific and found on sand or mud bottom in vary shallow area of estuary and near shore to depth of 25m. This species is dominant species of platycephalidae family, in Khuzestan, Bushehr and Hormozgan provinces and mainly is captured by bottom trawl, gillnet and moshta in Hormozgan. This study was designed to evaluate population variation and differentiation of bartail flathead (Platycephalus indicus (Linnaeus, 1785))in the Iranian waters of Persian Gulf using the morphometric and meristic characters and by AFLP marker. . A total 180 fish specimens were collected by gill net from six station(khor mosa, bahrekan, shif, motaf, charak and bandar abbas) that was 30 individual related to every station in Iranian shores of Persian Gulf . 28 morphometric factors and 11meristic specialties were measured and morphometric factors was standardized with Beacham formula. Univariate analysis of variance (One-way ANOVA) revealed significant differences with varying degrees between the means for 21 standardized morphometric measurements and 6 meristic counts that showed high significant differences between the six stations sampling. Discriminate function analysis (DFA) or the overall random assignment of individuals into their original groups was for morphometric and meristic characters was 47.9% and 53.9% respectively. The data were subjected to a principle component analysis (PCA) which grouped in eight and four factors for morphometric and meristic charactersrespectively.. Genetic diversity of six populations of bartail flathead (Platycephalus indicus) was investigated using amplified fragment length polymorphism (AFLP). A total of 118 reproducible bands amplified with ten AFLP primer combinations were obtained from 42 fishes that were collected from six different locations in the northern of Persian Gulf. The percentage of polymorphic bands was 57.06%. Average of Nei’s genetic diversity was 0.200±0.008, and Average of Shannon’s index was 0.300±0.011. The results of AMOVA analysis indicated that 66% of the genetic variation contained within populations and 34% occurred among populations and gene flow was 0.6454.The estimated level of population differentiation asmeasured by average Fst value across all loci was 0.327. Plotting discriminant functions 1 and 2 and UPGMA dendrograms based on Euclidian distance and genetic distance also showed at least five separate populations of bartail flathead in the northern Persian Gulf.

Seasonal foods, gonadal maturation, and length-weight relationships for nine fishes commonly captured by shrimp trawl on the Northwest Gulf of Mexico Continental Shelf

Recent emphasis on ecosystem approaches to fisheries management renews interest in, and the need for, trophic information about fish communities. A program was started in 1980 at the National Marine Fisheries Service Galveston Laboratory to develop a trophic database for continental shelf fishes. Collections were made during 1982-1983 that were processed but never published, yet the data remain valid today for historical purposes and for delimiting food web components within ecosystem assessments. I examined spring, summer, and fall foods in offshore populations of nine common species of trawl-susceptible fishes, with particular reference to predation on commercial penaeid shrimps (Farfantepenaeus and Litopenaeus). Diets were evaluated with the Index of Relative Importance (IRI) which combines the occurrence, number, and weight of each food item. Bank sea bass (Centropristis ocyurus) and bighead searobin (Prionotus tribulus) primarily consumed crabs, more so by larger than smaller fish. Inshore lizardfish (Synodus foetens) was almost entirely piscivorous. Ocellated flounder (Ancylopsetta ommata) consumed fishes, crabs, and stomatopods. Dwarf sand perch (Diplectrum bivittatum), blackwing searobin (Prionotus rubio), rock sea bass (Centropristis philadelphica), southern kingfish (Menticirrhus americanus), and red snapper (Lutjanus campechanus) fed mainly on shrimps. Most fish diets varied with respect to size (age), time of day, area sampled, depth, or season. Rimapenaeus and Sicyonia were the most frequently identified shrimp genera - only five Farfantepenaeus and no Litopenaeus were identified in almost 4,300 fish stomachs. I also examined gonadal development and documented fish length-weight relationships. Ripe gonads were most frequently found during summer in dwarf sand perch, during fall in ocellated flounder and bighead searobin, and during spring for other species, except no ripe red snapper or bank sea bass were collected. Rock sea bass was found to be a protogynous hermaphrodite, while dwarf sand perch is a synchronous hermaphrodite. Only ocellated flounder and southern kingfish exhibited sex-related differences in length-weight relationships. (PDF contains 40 pages.)

Life History, Diet, Abundance and Distribution, and Length-Frequencies of Selected Invertebrates in Florida Bay, Everglades National Park, Florida

This report presents information on the life history, diet, abundance and distribution, and length-frequency distributions of five invertebrates in Florida Bay, Everglades National Park. Collections were made with an otter trawl in basins on a bi-monthly basis. Non-parametric statistics were used to test spatial and temporal differences in the abundance of invertebrates when numbers were appropriate (i. e., $25). Invertebrate species are presented in four sections. The sections on Life History, and Diet were derived from the literature. The section on Abundance and Distribution consists of data from otter-trawl collections. In addition, comparisons with other studies are included here following our results. The section on Length-frequency Distributions consists of length measurements from all collections, except 1984-1985 when no measurements were taken. Length-frequency distributions were used, when possible, to estimate life stage captured, spawning times, recruitment into Florida Bay for those species which spawn outside the Bay, and growth. Additional material from the literature was added when appropriate. (PDF contains 39 pages)

Additional information on the length-weight relationship of skipjack tuna from the Eastern Tropical Pacific Ocean

ENGLISH: Results of a study of the length-weight relationships of yellowfin (Neothunnus macropterus) and skipjack (Katsuwonus pelamis) tuna from several fishing areas of the Eastern Tropical Pacific Ocean have been published by Chatwin (1959). In that report, a very low exponential value of 2.6261 was obtained for skipjack from Area 14 (off northern Chile, see Chatwin, Figure 1). It was pointed out, however, that this estimate was based on two samples of fish with a very narrow range of total lengths, not representative of the range in the catch, and that it would be desirable to obtain a further estimate based on a larger range of total lengths. In addition, there proved to be significantly large differences among exponents for the areas sampled, precluding use of a single regression equation for all areas. Two important fishing areas remained unsampled (Areas 10 and 13, see Chatwin, Figure 1), and it appeared desirable to collect length-weight measurement data from them, so that estimating equations would be available for all areas. Subsequent to publication of Chatwin's study, samples of skipjack length-weight measurements were obtained from the desired areas. Estimates derived from these data, and their effects on the previous analysis are presented herein. SPANISH:Los resultados de un estudio sobre las relaciones entre la longitud y el peso del atún aleta amarilla (Neothunnus macropterus) y del barrilete (Katsuwonus pelamis) de las diferentes áreas de pesca en el Pacífico Oriental Tropical ya han sido publicados por Chatwin (1959). En ese informe se obtuvo un valor exponencial muy bajo de 2.626 para el barrilete del Area 14 (frente a la costa norte de Chile, ver Chatwin, Figura 1). Se hizo hincapié, sin embargo, en que esta estimación se basaba en dos muestras de peces con una amplitud muy estrecha de longitudes totales, no representativa de la amplitud en la pesca, y que sería deseable obtener una estimación adicional basada en una amplitud mayor de longitudes totales. Además, se comprobó que habian diferencias significativamente grandes entre los exponentes de las áreas muestreadas lo que impedía el usa de una sola ecuación de regresión para todas las áreas. Se quedaron sin muestrear dos importantes áreas de pesca (Areas 10 y 13, ver Chatwin, Figura 1) y pareció deseable recolectar datos de medidas de longitud y peso de estas áreas, de tal manera que hubiesen disponibles ecuaciones estimadoras para todas las áreas. Después de la publicación del estudio de Chatwin, so obtuvieron muestras de medidas de longitud y peso de barriletes de las áreas deseadas. Las estimaciones derivadas de estos datos y sus efectos sabre el análisis previo se dan en el presente informe.

The relationships between length and weight of yellowfin tuna (Neothunnus macropterus) and skipjack tuna (Katsuwonus pelamis) from the Eastern Tropical Pacific Ocean

ENGLISH: The purposes of the Convention which established the Inter-American Tropical Tuna Commission have been discussed in the Commission's Annual Report for 1950-51 (Schaefer, 1952) and in subsequent Annual Reports. One of its functions is to gather and report on biological information for the tunas of the Eastern Tropical Pacific, the yellowfin tuna (Neothunnus macropterus) and skipjack tuna (Katsuwonus pelamis). This report gives the results of a study of the relationships between the length and the weight of individuals of each of these species, derived from samples of fish of commercial sizes captured during the course of regular fishing operations in several areas of the Eastern Tropical Pacific Ocean SPANISH: Los propósitos de la Convención que estableció la Comisión Interamericana del Atún Tropical han sido expuestos en el Informe Anual de dicho organismo correspondiente a 1950-51 (Schaefer, 1952), y en los siguientes informes anuales. Una de sus funciones es la de recoger información biológica sobre los atunes del Pacífico Oriental Tropical, es decir, el aleta amarilla (Neothunnus macropterus) y el barrilete (Katsuwonus pelamis) y darla a conocer a las partes interesadas. El presente trabajo informativo proporciona los resultados de un estudio de las relaciones entre la longitud y el peso tomados en individuos de cada una de estas especies, mediante muestreo en pescados de tamaños comerciales capturados en el curso de operaciones regulares de pesca, en diversas área del Océano Pacífico Oriental Tropical.

Relationship between fish size and otolith length for 63 species of fishes from the Eastern North Pacific Ocean

Otoliths commonly are used to determine the taxon, age, and size of fishes. This information is useful for population management, predator-prey studies, and archaeological research. The relationship between the length of a fish and the length of its otoliths remains unknown for many species of marine fishes in the Pacific Ocean. Therefore, the relationships between fish length and fish weight, and between otolith length and fish length, were developed for 63 species of fishes caught in the eastern North Pacific Ocean. We also summarized similar relationships for 46 eastern North Pacific fish species reported in the literature. The relationship between fish length and otolith length was linear, and most of the variability was explained by a simple least-squares regression (r 2 > 0.700 for 45 of 63 species). The relationship between otolith length and fish length was not significantly different between left and right otoliths for all but one fish species. Images of otoliths from 77 taxa are included to assist in the identification of species. (PDF file contains 38 pages.)

Length-weight relationships of the anchoveta, Cetengraulis mysticetus, in the Gulf of Panama

ENGLISH: Knowledge of the length-frequency distribution and the length-weight relationships of the anchoveta is essential for converting the catch statistics of that species from pounds to numbers of fish. Such conversions are necessary for various types of investigations, especially those involving estimation of the population size and mortality rates. The analysis of the results of a recent tagging program conducted with anchovetas in the Gulf of Panama (Bayliff, 1965) requires that such conversions be made. Length-frequency data for the anchoveta have already been collected and published (Howard and Landa, 1958; Bayliff, 1964). The present report deals with length-weight data from fish collected in various .areas of the Gulf of Panama in all months of the year and in several different years. Opportunity is thus afforded to compare the length-weight relationships of fish of different year classes in different areas, months, and years. SPANISH: El conocimiento de la distribución de la frecuencia de longitud y de las relaciones entre la longitud y el peso de la anchoveta es esencial en las estadísticas de captura de esta especie para la conversión de peso en libras a número de peces. Estas conversiones son necesarias para varios tipos de investigación, especialmente las que se refieren a la estimación del tamaño de la población y de las tasas de mortalidad. Estas conversiones se requieren para analizar los resultados de un programa reciente de marcación de anchovetas en el Golfo de Panamá (Bayliff, 1965). Los datos de la frecuencia de longitud de la anchoveta ya han sido recolectados y publicados (Howard y Landa, 1958; Bayliff, 1964). El presente informe trata de los datos de longitud y peso de los peces recolectados en varias áreas del Golfo de Panamá durante todos los meses del año y durante varios años. Esto ofrece la oportunidad de comparar las relaciones entre la longitud y el peso de peces pertenecientes a varias clases anuales en áreas, meses y años diferentes.

Length-weight relationship and the diets of Clarias lazera (Cuvier and Valenciennes), family Clariidae (Osteichthyes: Siluriformes) in Zaria, Nigeria

The length-weight relationship and the diets of Clarias lazera were investigated between July 1981 and June 1982. About 450 specimens were examined. The standard lengths of the fish ranged from 8.5 cm to 42.2 cm. Significant differences were found between the standard lengths of the males and females with the latter slightly shorter. Somatic weights varied between 10 g to 502 g. Length-weight regression analysis gave a "b" value of 3.02 for both males and females combined; thus indicating an isometric growth. Analysis of the food in the stomachs showed that the fish is an omnivore although, it fed more on insects and fish than other food items

A study of the length at maturity and hooks/gill net mesh selection in Clarias lazera from Lake Chad

The minimum length at first maturity of Clarias lazera was found to be 24 cm (4.8%) for females and 20 cm (1.8%) for males. Fifty percent maturity was attained at length of 28 cm to 30 cm for both sexes; there being little difference among the sexes at this level of maturity. The modal retention lengths for gill nets were: 13 cm for 25.5 mm mesh; 18 cm for 32 mm mesh; 28 cm for 57 mm mesh; and 38 cm for 76 mm mesh. Modal lengths of Clarias lazera caught by various hooks sizes were No. 10 (28 cm); No. 11 (33 cm); Nos. 15 and 16 (28 cm). It is recommended that to protect the clarias fishery in Lake Chad, the use of gill nets of less than 57 mm mesh size and fishing hook No. 16 (and smaller sizes) which caught 43.94% of immature fishes should be discouraged

Length-frequency estimation for yellowfin tuna (Thunnus albacares) caught by commercial fishing gear in the Eastern Pacific Ocean

ENGLISH: Methods of collecting samples for the purpose of estimating the numbers and weights of fish caught, by length interval, are described. Several models for two-stage sampling are described, and the equations for the estimators and their variances are given. The results from a brief simulation study are used to show the differences between estimates made with the different models. Estimators for the average weights of fish in the catch and their variances are also described. These average weights are used to provide improved estimates of the total annual catches of yellowfin taken from the eastern Pacific Ocean, east of 150°W, between 1955 and 1990. SPANISH: Se describen los métodos de recoger de muestreo para estimar el número o peso de peces capturados, por intervalo de talla. Se describen varios modelos para el muestreo de dos etapas, y se presentan las ecuaciones para los estimadores y sus varianzas. Se usan los resultados de un breve estudio de simulación para indicar las diferencias entre estimaciones realizadas con los distintosmodelos. También se describe un estimador para el peso promedio de peces en la captura y su varianza. Se usan estos estimadores para calcular estimaciones mejoradas de las capturas anuales totales de aleta amarilla tomadas del Océano Pacífico oriental, al este de 150°W, entre 1955 y 1990. (PDF contains 41 pages.)

Growth and age composition of northern bluefin tuna, Thunnus thynnus, caught in the Eastern Pacific Ocean, as estimated from length-frequency data, with comments on trans-Pacific migrations

ENGLISH: The spawning of Pacific northern bluefin tuna, Thunnus thynnus, takes place only in the western Pacific Ocean (WPO), but substantial numbers of the juveniles migrate to the eastern Pacific Ocean (EPO), where they remain for several months, or longer, and the.n return to the WPO. Lengthfrequency and tagging data show that many bluefin arrive in the EPO as 1-and 2-year olds, and remain there for one or two fishing seasons before returning to the WPO. The proportion of the fish which make the west-to-east migration varies among years. The numbers of 1-, 2-, 3-, 4, and >4 –year olds in the catches of the EPO are estimated for most years of the 1952-1991 period. SPANISH: EI desove del atun aleta azul del norte del Pacifico, Thunnus thynnus, ocurre solamente en el Océano Pacifico occidental (WPO), pero números substanciales de los juveniles migran al Océano Pacifico oriental (OPO), donde permanecen unos meses, 0 mas, antes de regresar al WPO. Datos de marcado y frecuencia de talla indican que muchos aletas azules llegan al OPO a 1 o 2 anos de edad, y permanecen alIi una 0 dos temporadas de pesca antes de regresar al WPO. La proporcion de los peces que migra del oeste al este varia entre anos. Se estima el numero de peces de 1, 2, 3, 4, Y>4 anos de edad en las capturas del OPO para la mayoria de los anos del periodo de 1952-1991. (PDF contains 40 pages.)