Production and conservation of nutrient-rich small fish (SIS) in ponds and wetlands for nutrition security and livelihoods in South Asia: Proceedings of a World Bank/SAFANSI funded regional workshop on small fish and nutrition, Dhaka, Bangladesh, 1st - 2nd March 2014

Small indigenous fish species (SIS) are an important source of essential macro- and micronutrients that can play an important role in the elimination of malnutrition and micronutrient deficiencies in the populations of many South and Southeast Asian countries. Of the 260 freshwater fish species in Bangladesh, more than 140 are classified as SIS and are an integral part of the rural Bangladeshi diet. As many SIS are eaten whole, with organs and bones, they contain high amounts of vitamins and minerals, including calcium, and iron and zinc. Some SIS, such as mola, are also rich in vitamin A. SIS are often cooked with vegetables and a little oil, so they contribute to the food diversity of the rural poor.SIS are recognized as a major animal-source food group, contributing to improved food and nutrition security and livelihoods of the people of South and Southeast Asia. The purpose of this workshop is to bring together policy makers, extension agents, researchers, non-governmental and development organizations to share knowledge about small fish, their contribution to better nutrition, production technologies, and strategies for wider dissemination of pond culture and wetland based-production and conservation technologies. The workshop is expected to generate ideas for further research and development of sustainable technologies for production, management and conservation of SIS for the benefit of the people of Bangladesh as well as the South and Southeast Asian region.

Nutritional value of some small indigenous fish species (SIS) of Bangladesh

Twenty three small indigenous fish species (SIS) in the size range of 3-18 cm were analyzed for proximate composition and minerals (Ca and P) content to evaluate their nutritive value. The moisture content of different species ranged between 71.00 and 81.94%. In general, small sized fishes showed higher moisture content. The muscle protein content among the species varied widely (16.16-22.28%). In general, the muscle protein content of fishes showed higher value than the whole carcass protein content. The carcass lipid content varied between 1.87 and 9.55% and showed an inverse relationship with the moisture content. The gross energy content ranged from 19.51-27.30 KJ/g on dry matter basis. In the present study, the calcium and phosphorus contents ranged between 0.85-3.20% and 1.01-3.29% respectively. The calcium and phosphorus ratio (Ca/P) varied between 0.44 and 2.00. From the nutritional point of view, it shows that the SIS are good source of protein and minerals especially calcium and phosphorus

Present status of harvesting, transportation and marketing of freshwater Small Indigenous Species of Fish (SIS) of Bangladesh

A survey conducted to evaluate the present status of harvesting, transportation and marketing of freshwater SIS of Bangladesh showed that among the 56 to 73 species of small indigenous fish species (SIS), mola (Amblypharyngodon mola), dhela (Osteobrama cotio cotio), puti (Barbodes sarana), tit puti (Puntius ticto), tengra (Mystus vitatus), chapila ( Gudusia chapra), batashi (Pseudotropius atherinoides), buzuri tengra (Mystus tengra), katchki (Corika soborna), gol chanda (Chanda ranga), lamba chanda (Chanda nama), phul chela (Chela phulo) and lata (Channa punctatus) are the most common. But the abundance of the mola, puti, tengra, batashi and chapila are higher than the other SIS species. Puti was at the top of the list in percentage abundance (7-9%), followed by mola (7-8%), tengra 6-7% and chapila 5-6% in all the investigated areas. Three levels of market or marketing systems were observed in the distribution channel of fish trade: primary, secondary/higher secondary and final consuming markets. Primary market is the catching point in the rural area. In secondary market, the collectors bring the fish from primary market to the landing ghats, usually to the nearest thana market or at a place well linked by rivers, road and rail transport. On purchasing the fish from the higher secondary market, the paikars sell the fish to the retailers who take it to the final consumer market. Different types of crafts and gears are used for catching the SIS in Bangladesh. Monofilament gill nets (20%), traps and line nets (25%) and seine nets (25%) are the dominant gears in respect of the total catch followed by lift net (10%) and cast net (20%). A total of 10 types of gear are listed according to their mode of operation which used for catching SIS. The processors in consumer market received higher marketing profit followed by primary market and secondary market, respectively.

Impact of small indigenous species of fish (SIS) on livelihood of local fishing community in two upazilas of Mymensingh

The fisher folk used to catch small indigenous species of fish (SIS) from rivers, canals, wetlands and floodplains at little or no cost for their livelihood. Surplus fish was sold at the local market to generate some little capital for the households. The livelihood and consumption of SIS in fishing community of two upazilas viz. Trisal and Ishwarganj under Mymensingh district were studied for 3 months in 2004. Most of the fisher folk of the study areas belong to resource-poor section of the society living below the poverty level. Majority of them had no cultivable land. As professional fishers they face many problems during lean fishing period from January to April due to little or non-availability of fish. Majority of the fisher households consumed SIS three to four days a week. The fisher households of Trisal upazila consumed more small fish than those of Ishwargonj upazila. More than 50% respondents consumed <20 g SIS/day and 20% consumed >40 g SIS/day in Trisal upazila. On the other hand, in Ishwargonj upzila, most of the fisher households (66%) were found to consume <20 g SIS/day. SIS was mostly available from July-December in rivers, wetlands (beels), and canals, and income from fishing was reported to be good. The dominant SIS was Puntius spp., Mystus spp., Anabas testudineus, catfishes, mola, and small prawns. Non-indigenous species like tilapia was also dominant in Trisal upzila where aquaculture practices were well established.

Marketing of small indigenous species of fish (SIS) in three selected fish markets of Mymensingh

A socio-economic survey was conducted round the year in three fish markets at Mymensingh, Bangladesh. The selected markets were categorized as rural market (Sutiakhali market), a peri-urban market (Kamal Ranjeet market, BAU) and an urban market (Notun Bazar market, Mymensingh town). It was learnt from the survey that the availability of Small Indigenous Fish Species (SIS) declined to a great extent over the last few years and at presently many of such fish species are either threatened or at the edge of extinction. The supply of SIS was highest in KR market (37% of total) and more or less similar in Notun Bazar and Sutiakhali fish market (25 and 27% respectively). The total supply of SIS fluctuated from 25% to 35% throughout the year in these markets. About 48 SIS were found in the sampled markets over the survey period. The highest number of species (45) was found in KR market followed by Notun Bazar (42) and Sutiakhali (37) fish markets. During the survey, three critically endangered species namely, schilbid catfish, garua catfish and rita were found in these markets. Beside these, other 11 and 10 species were listed to be endangered and vulnerable respectively. The biodiversity of 21 SIS found in three markets were no threat at all. Three species (guntea loach, Indian glass barb and flying barb) were 'data deficient' as reported by the IUCN Red Book (IUCN-Bangladesh 2000). From the supply point of view small prawn, spotted snakehead, stinging catfish, pool barb, striped dwarf catfish, Gangetic mystus, walking catfish and tank goby were the prominent fish. The least available species found in this survey were lesser spiny eel, barred spiny eel, Gangetic ailia, freshwater garfish, zig-zag eel, flying barb, Ganges river sprat, freshwater river shad and dwarf gourami. The weight of SIS available in Notun bazar was highest and nearly double than other two markets. There was no significant difference recorded in the supply of SIS in Sutiakhali and KR markets. The average monthly SIS supply was 185, 192 and 467 kg in KR, Sutiakhali and Notun Bazar, respectively; therefore, the cumulative average supply was 844 kg per month in three markets. The price of SIS ranged widely from taka 50-450/kg depending on species, location of market, time of purchase and the condition of fish. In general small prawn, ticto barb, dwarf gourami, Gangetic leaffish, and Annandale loach were sold at a lower price (ranged taka 50-100/kg) and these species could be considered at the bottom of the market-price list. Other SIS like walking catfish, climbing parch, butter catfish, cotio and schilbid catfish valued as highest price (ranged taka 150-450/kg). There was no specific marketing chain for SIS in Mymensingh region. The components of marketing channels and their expansion varied with seasons and locations. The general pattern, however, was as this - after buying fish from fish farmer/fishermen, middlemen (locally known as Foria) used to buy fish to wholesale market and sell to the wholesalers. The retailers used to buy fish from wholesaler through auction to the highest bidders. The retailers then send the fish to particular market where the fish reached the consumers. The livelihood strategy of SIS retailers in three fish markets showed that socio-economic constraints such as low income, poor educational background, low economic status and lack of capital are the main constrains [sic]. Most of the retailers proposed that government should control the fish price throughout the year, so that the producers can get reasonable and stable price. Construction of cold storage and preservation facilities at market sites, improvement of road and communication, improvement of physical market facilities and reduction of market chain is essential. Credit facilities, improvement of their standard of living, health and sanitary condition, housing condition, children education and access to drinking water facilities were identified as additional aspects to improve socio-economic condition of SIS retailers.

Quality assessment of traditional and solar tunnel dried SIS (Small Indigenous Fish Species) products

Studies were conducted to assessment the quality of traditional and solar tunnel dried SIS products. The moisture content of the solar products ranged from 14.38 to 18.48% with the lowest in batashi and the highest value in tengra. The moisture content of the traditional products was in the range of 23.26 to 26.42%. The range of protein contents on moisture free basis was from 67.57 to 71.90% in solar dried fishes with highest value obtained in dhela and lowest value in batashi. These values were more or less similar to those of traditional dried SIS products which were in the range of 68.02 to 73.54% on dry weight basis. Lipid contents of solar dried SIS varied from 14.10 to 16.26% and on moisture free basis the in the range of 11.73 to 21.98 with highest value found in tengra and lowest in puti. These values were more or less similar to those found for traditional dried products on dry weight basis and ranged from were 12.37 to 22.43%. Maximum reconstitution of solar dried products was obtained at 80°C in all samples and was in the range of 65.26 to 70.51% where the percentage of reconstitution increases with the increase of socking time and reach maximum at the end of up to 60 min. The TVB-N content of solar dried fish is low compared with traditional one ranging from 20.30 to 28.40mg/100g and peroxide value in the range of 12. 54 to 19.20meq./kg oil. The TVB-N of traditionally dried products were in the range of 32.50 to 45.45mg/100g and PO values of the traditionally dried products were in the range of 30.00 to 36.00meq./kg oil. The bacterial load of the solar dried products was in the range of 4.0x10 super(3)/g to 3.6x10 super(5)CFU/g and of the traditionally dried products ranged from 1.45x10 super(5) to 2.52x10 super(6) CFU/g.

Shelf-life of rotary and solar tunnel dried SIS products under different packaging and storage conditions

A study was conducted on the shelf-life of rotary and solar tunnel dried SIS products under different packaging and storage conditions. Organoleptically dried products were found in good condition after a storage period of 60 days in ambient and chilled conditions. The moisture content, TVB-N value and bacterial load slightly increased during 60 days of storage in ambient and chilled conditions. The changes in moisture content and bacterial load were faster in ambient temperature than in chilled storage condition whereas changes in TVB-N value was higher in chilled condition than in ambient temperature. The initial moisture content was in the range of 13.71% to 22.84%. After 60 days of storage in ambient and chilled condition the moisture content of dried products was in the range of 15.09% to 25.11% and 14.49% to 25.01%, respectively. The initial TVB-N value was in the range of 10.64 to 17.52 mg/100g and after 60 days of storage in ambient and chilled condition, TVB-N value was in the range of 29.00 to 34.82 mg/100g and 31.41 to 39.11 mg/100g, respectively. The initial bacterial load was in the range of 1.91x10 super(8) to 2.84x10 super(8) and after 60 days of storage in ambient and chilled condition, the bacterial load was in the range of 6.2x10 super(8) to 1.8x10 super(9) and 5.75x10 super(7) to 5.05x10 super(8) CFU/g, respectively. The results of the present study indicated that it is necessary to store high quality dried products in sealed packed in chilled condition to ensure good quality up to a certain period of time.

The role of small indigenous fish species in food and nutrition security in Bangladesh

In Bangladesh, only 6% of the daily food intake is animal food of which fish accounts for 50%. Rice is the mainstay, making up 60% of the daily food intake. However, many nutrients such as vitamins A and C, iron, calcium, zinc and iodine are not found in rice and have to be obtained from other sources. Small indigenous fish are a vital contribution to the diet of the rural poor in Bangladesh, where more than 30,000 children go blind every year from vitamin A deficiency and 70% of women and children are iron-deficient. Small fish, which are less than 10 cm in length and usually eaten whole with the organs and bones, contain large amount of calcium and possibly iron and zinc. The largest fish promoted in aquaculture do not contribute significantly to calcium intake. Some species also contain large amount of vitamin A. Much of the small indigenous fish (SIS) of Bangladesh are caught in floodplains and natural waterbodies. Small fish are eaten frequently in small amounts and are more equally distributed among family members than big fish of which men get the larger share. Unfortunately, overfishing and the deterioration of natural habitats have resulted in a decline in SIS. When measures are taken to improve food and nutrition security, there should be a focus on production of small fish so that greater quantities are accessible for consumption by the rural poor.

Culture potentials of mola (Amblypharyngodon mola ), chela (Chela cachius) and punti (Puntius sophore) under monoculture system

To assess the production potentials of small indigenous fish species (SIS) in semi-intensive monoculture system, an experiment was carried out with Amblypharyngadan mala, Chela cachius and Puntius sophore. Three treatments each with three replications were tested with mola, chela and punti individually and the stocking density of each species was 100,000/ha. Organic fertilizer was applied to the ponds at the rate of 1,000 kg/ha at fortnightly and rice bran was supplemented daily at 3% of the total fish biomass. Partial harvesting was made after three months of stocking. Gross production of 805±52.94, 1,120±41.62 and 509±48.81 kg/ha, respectively for mola, chela and punti over a period of six months were obtained. The yield of punti was found to vary significantly (P < 0.05) from that of mola and chela.

Impact of community-based fisheries management practices on production and species diversity in Turag-Bangshi basin floodplains in Kaliakoir, Gazipur, Bangladesh

The study was conducted to investigate the communities perception and compliance to community-based fisheries management (CBFM) in Turag-Bangshi floodplains under Kaliakoir, Gazipur District. Measures such as ban on use of the harmful fishing gears, seasonal fishing closure, halt of fry fishing, halt of dewatering of beels and the impact of establishment of sanctuaries on fish production and species diversity were introduced by MACH project. Almost all members of the communities in Turag-Bangshi MACH (Management of Aquatic Ecosystem through Community Husbandry) site welcomed the introduction and complied with the implementation of all management measures which helped stopped use of harmful fishing gears, ensured survival and breeding of brood fish in the rainy season, protected and allowed fry to grow big, restored lost and degraded fisheries and organized communities for sustainable development of the fisheries. A total of 51 species of fishes were found in Makosh beel (natural depression). Among these, small indigenous species (SIS) under Cyprinidae family (Puntius sophore) was the most dominant. Many species available in the past recorded disappeared from the Makosh beel due to loss of habitat and industrial pollution that damaged spawning and nursery grounds of fish. Introduction of some selective native endangered species (Nandus nandus, Notopterus notopterus, Ompok pabda and Labeo calbasu) by MACH in the Turag-Bangshi water bodies increased diversity of species from 82 to 95. Over a period of five years during MACH intervention, the average production remained nearly 200% higher than the baseline production of 57 kg/ha to present 207 kg/ha due to maintaining sanctuaries and the closed fishing seasons. Per capita daily fish consumption of the surrounding communities also increased by 78% (from 27 to 48 g/person/day) which is much higher than the national average fish consumption in Bangladesh. The implementation of community-based MACH project management measures substantially improved fish habitat, production, consumption and socio-economic conditions of the surrounding communities. The model can be used to improve the floodplains of Bangladesh.

Culture potentials of bata, Labeo bata under semi-intensive management system

Small indigenous fish species (SIS) provide food, nutrition, subsistence and supplemental income to a great majority of the people particularly the poor and disadvantaged people of Bangladesh. To date nobody explored the possibilities of culturing these species in combination with the indigenous major carps viz. catla, Catla catla; rohu, Labeo rohita, and mrigal Cirrhinus cirrhosus and grass carp, Ctenopharyngodon idellus. An experiment on the polyculture of carps with a popular SIS, bata (Labeo bata) was carried out to evaluate the production performance of carp-SIS culture in on-farm condition during 15 March to 15 September 2003. Three treatments each having three replications with different stocking densities of bata were tested keeping the carp species combination and stocking density similar in all the treatments viz. treatment 1 (T1), bata (5,000/ha) + catla, rohu and mrigal (5,000/ha at the ratio of 1:1:1) + grass carp (250/ha); treatment 2 (T2), bata (7,500/ha) + catla, rohu and mrigal (5,000/ha at the ratio of 1:1:1) + grass carp (250/ha); and treatment 3 (T3), bata (1 0,000/ha) + catla, rohu and mrigal (5,000/ha at the ratio of 1:1:1) + grass carp (250/ha). After six months rearing, the production obtained were 2,466±77, 2,395±85 and 2,074±72 kg/ha from T1, T2 and T3, respectively. The highest production was obtained from T1, where the stocking density of bata was the minimum (5,000/ha) while the lowest production was obtained from T1 [sic], where the stocking density of bata was maximum (10,000/ha). Significant difference (P<0.05) exists in the production levels as obtained from different treatments. The contribution of bata to total production was 10.31%, 13.96% and 14.38% in case of T1, T2 and T3, respectively.

Shelf life of dried products from small indigenous fish species under various packing and storage conditions

The overall quality of five SIS products was found in good condition up to 2 months storage on the basis of organoleptic, biochemical and bacteriological characteristics and all the products was excellent in sealed packed condition up to 45 days of storage. However, quality of the products stored in open air atmospheric temperature was found excellent for first 15 days. In an average the initial moisture content was in the range of 13.5 to 15.0% with highest moisture content in puti and lowest in chapila. At the end of the 60 days the moisture content reached to the range of 18.5 to 19.0% which was more or less near the recommended limit of 16% for dried fishery products. The moisture content beyond the recommended limit as the storage period increased further and at the end of 90 days the moisture content increased to the range of 22.9 to 24% when organoleptically the product quality became very poor. The changes in the value of total volatile base nitrogen (TVB-N), peroxide value (PO), moisture and aerobic plate count (APC) of solar tunnel dried products in sealed polythene packages were investigated during 60 days of storage. There was little or no differences in TVB-N, PO and bacterial load of each species packed under various polythene density. The initial TVB-N values were in the range of 10.30 to 12.40 mg/100g of the samples. TVB-N value increased slowly up to the end of the storage period and was to in the range of 46.20 to 57.00 mg/1 00 g of sample. Initially the peroxide values (P.O.) were in the range of 6.54 to 8.40 m.eq./kg oil of the samples. During 60 days of storage, P.O. values increased slowly and at the end of the storage period these values reached to the range of 22.00 to 25.30meq./kg of sample. The initial APC was in the range 5.3xl04-7.3x104 CFU/g. The bacterial load increased slowly and at the end of the 60 days storage period reached to the range 6.6x106 - 8.6x107 CFT/g.

Quality assessment of traditional, rotary and solar tunnel dried small indigenous fish species products

Studies on the quality assessments of three traditional, rotary and solar tunnel dried SIS products were conducted. Organoleptic quality of traditional dried SIS products available in the markets was poor compared to those produced in rotary and solar tunnel dryer. Reconstitution of samples were in the range of 54.26% to 75.24%, 69.37% to 83.73% and 55.08% to 80.24% when soaked at 80°C for traditional, rotary and solar tunnel dried products, respectively. The percentage of reconstitution increased with the increase of soaking time and the uptake of water was maximum after 60 min of soaking. The moisture contents of traditional, rotary and solar tunnel dried products were in the range of 26.02% to 27.33%, 16.23% to 22.84% and 13.71% to 19.30%, respectively. The protein contents were in the range of 60.78% to 72.59%, 62.17% to 76.27% and 61.11% to 76.00%, respectively; lipid contents were in the range of 12.26% to 22.60%, 14.00% to 24.71% and 13.92% to 22.39%, respectively and ash contents in the range of 15.11% to 16.59%, 8.32% to 13.51% and 8.71% to 16.45%, respectively on dry matter basis. The TVB-N content of rotary and solar tunnel dried products was low compared to traditional one ranging from 10.64 to 17.52 mg/100g and 14.34 to 15.68 mg/100g, respectively whereas the TVB-N content of traditional samples was in the range of 15.46 to 20.36 mg/100g. The bacterial load of traditional, rotary and solar tunnel dried products were in the range of 1.43x10 super(8) CFU/g to 2.89 x10 super(80 CFU/g, 1.91x10 super(8) CFU/g to 2.84x10 super(8) CFU/g and 1.95x10 super(8) CFU/g to 2.59x10 super(8) CFU/g, respectively. The results of the study indicated that dried fish products from rotary dryer and solar tunnel dryer were found to be of better quality in nutritional and food quality aspects than those of traditional dried products.

Polyculture of carp with small indigenous fish, bata, Labeo bata (Ham.) at different stocking densities

Small indigenous fish species (SIS) play very important role in the diet of the people of Bangladesh. Until recently, the possibilities of culture them in consideration with the Indian major carp yet to be explored. In view of the above, an experiment on the polyculture of carps with SIS, bata (Labeo bata) was carried out to evaluate their production performance in the on-farm condition during 15 March to 15 September 2003. Three different stocking densities of bata with carp species were given. After six months of rearing, the productions obtained were 2,466±98 kg/ha, 2,395 ±88 kg/ha and 2,074±94 kg/ha from treatments-1, 2 and 3, respectively. The highest production was obtained from treatment-1, when compared with treatments-1 and 2. The contribution of bata in terms of production was 10.31% in treatment-1, while it was 13.36% and 14.38% in treatments-2 and 3, respectively.

Production of quality dried small indigenous fish species products using low cost solar tunnel drier

A low cost solar drier was constructed using locally available materials. The size of the drier was 20x3.6x3 having drying capacity of 80 kg of SIS (w/w). Optimization of moisture content was observed for mola, dhela, chapila, chanda and puti at temperature ranges between 40-45°C and 50-55°C in solar tunnel drier. There was little or no change in moisture content at temperature below 40°C during the first 3 hours. Then the moisture content declined gradually with the increase of drying period. On the other hand, at temperature between 50-55°C, moisture content started to decline after 2 hours of drying. The moisture content of the sample reached at about 16% after 26 hours of sun drying at 40-45°C and 20 hours at 50-55°C. The optimum temperature for producing high quality dried products was 45-50°C in solar tunnel drier. The temperature and relative humidity outside and inside the dryers (with fish) at various locations were recorded from 8.00am to 4.00pm. The normal atmospheric ambient temperature was recorded in the range of 25-37°C from at 8:00am to 4:00pm. During the same period the atmospheric relative humidity recorded was in the range of 30-58%. On the other hand, the maximum temperature inside the dryers was recorded in the range of 28-65°C. The lowest temperature recorded was 28°C in the morning and at 13.00pm the highest temperature 65°C was recorded. The maximum relative humidity 58% found in the afternoon and minimum of 28% at noon. There was inverse relationship between temperature intensity of sunshine and humidity which decreased as sunshine increased. In total, it took around 26 hours of drying to reduce the moisture level to about 16%.