Salmon stocks and fisheries in England and Wales, 2001. Preliminary assessment prepared for ICES, April 2002

This report presents a preliminary assessment of the state of salmon stocks and fisheries in England and Wales in 2001 to assist ICES in providing scientific advice to NASCO and to provide early feedback to fishery managers and anglers. The chief indicators of the state o f salmon stocks are normally the catches taken by rod and net fisheries. However, in 2001 angling was affected by the outbreak of foot and mouth disease (FMD), which restricted angling opportunities and access to rod fisheries in many parts of the country for lengthy periods. It is impossible to quantify the impact that FMD had on rod catches, although these were undoubtedly significantly reduced; net fisheries were unaffected by FMD. The declared salmon catch for 2001 (including those fish released alive by anglers) is provisionally estimated at 209 tonnes, representing some 57,000 fish, and comprising 153 tonnes (-43,000 fish) by nets and fixed engines and 56 tonnes (-14,000 fish) by rods. For direct comparison with previous years, it should be noted that the declared catch prior to the issue of a second reminder was about 49 tonnes (see below). An estimated 26 tonnes (43%) of the rod catch was released alive. These figures do not take account of catches of salmon which go unreported (including those taken illegally), and it is estimated that there may have been a total of 33 tonnes of additional fish caught in 2001; approximately 15% of all fish killed.

Relationship between total length and mouth gap of some commercially important carp fry

The estimated regression equation for total length and mouth gape computed were Log TL = Log 0.23 + 0.663 log MG (vertically) (r = 0.960) and Log TL = Log 0.08 + 0.686 log MG (horizontally) (r = 0.949). In case of rohu average total length from 11350 mm to 23775 mm and mouth gape 805 um to 1225 um (vertically) and 700 um to 1110 um (horizontally) between the first day of mouth opening up to 15 days. The regression equation for total length and mouth gap were Log TL = Log 0.20 + 0.660 log MG (vertically) (r = 0.935) and Log TL = Log 0.02 + 0698 log MG (horizontally) ( r = 0.907). In case of silver carp average total length from 12800 ,urn to 33555 um and mouth gape 690 um to 1210 um (vertically) and 615 um to 1115 um (horizontally) between the first day of mouth opening up to 15 days. The regression equation for total length and mouth gape were Log TL = Log 0.36 + 0.596 log MG (vertically) (r = 0.936) and Log TL = Log 0.26 + 0.607 log MG (horizontally) (r = 0.891). The relationship between total length and mouth gape (vertically and horizontally) of the studied fry were found to be linear and highly significant.

Study on regional differences in soil-water characteristics and their relevance to aquaculture productivity and fish disease

A total of 45 ponds used for fish polyculture were investigated in three zones of Bangladesh to identify the differences among the zones in respect to aqua-ecology, culture practices, fish productivity and health management. Four hundred and fifty fish from three zones were clinically examined by naked eye and histopathology. Out of total number of fish examined, 45 fish from Dhaka zones were examined for parasites and bacteria in addition to histopathology. Faded and haemorrhagic gill, skin, fin, scale loss and lesions were observed during fish examination. Aeromonas spp. Pseudomonas spp. and Streptococcus spp. were isolated respectively from 56%, 46% and 39% affected fish. Among the five water quality parameters analyzed, the highest average hardness and alkalinity respectively were recorded in Rajshahi (156 ppm and 142 ppm) followed by Dhaka (146 ppm and 132 ppm) and Chittagong (81 ppm and 90 ppm). The highest average pH was recorded in Mymensingh (7.52) followed by Rajshahi (7.13) and Chittagong (7.05). Water holding capacity of soil in Rajshahi zone was poor compared to other zones and farmers were found to be reluctant to fish farming.

The distribution of cirripeds and gastropods on plain vertical rock surfaces in the upper intertidal and splash zones

This is a student paper done for a University of California Berkeley Zoology class. Since UCB didn't have its own marine lab at the time, it rented space at Hopkins Marine Station where this work was done. Cadet Hand earned his Ph.D. from Berkeley and went on to become Director of the Bodega Marine Laboratory. Donald Putnam Abbott also earned his Ph.D. from Berkeley and later became a Stanford professor at Hopkins Marine Station. (PDF contains 26 pages)

Distribution and occurrence of bacterial fish diseases in the different cultural facilities of Lake Kainji area

Fish farming practices in the Lake Kainji Area of Nigeria are categorized under seven main cultural facilities, namely, earthen ponds/reservoirs, indoor/outdoor concrete tanks, plastic tanks, floating cages/hapas, aquaria, sewage and feral conditions. The presence of Bacteria isolates associated with diseased fish conditions varied significantly (P<0.05) with different cultural facilities. The highest bacteria isolates and bacterial disease incidence, 33% and 46% respectively, was associated with diseased fish in the indoor/outdoor concrete tanks. The least incidence of bacteria isolates (3.5%) and blue bacterial disease (3%) was associated with diseased fish in the aquaria and feral conditions. Nine Gram-negative and two Gram-positive bacteria genera were isolated during this investigation. Pseudomonas spp. (23.6%) and Staphylococcus spp. (14.3%), were the predominant Gram-negative and Gram-positive bacteria genera in the different cultural facilities, respectively. This paper highlights the relevance of occurrence and distribution of bacteria isolates associated with diseased fish to bacterial fish diseases under different cultural facilities

Otto Kelland’s Model Dories and the Digital Archives Initiative

Otto Kelland was a truly unique individual in Newfoundland. During his long life he had several careers from being a prison superintendant to being an instructor at Marine Institute. During his life Kelland made hundreds of wooden boat models. They are beautifuly hand-crafted and represented the type of watercraft used by fishermen in Newfoundland. The collection of boat models made by Otto Kelland and owned by Marine Institute made an ideal object to be digitalized. In particular the collection of dories was an ideal group to be digitized. They were housed in one cabinet and accompanied by hand-written documents describing each model. The Digital Archives Initiative (DAI) is a “gateway to the learning and research-based cultural resources held by Memorial University of Newfoundland and partnering organizations.” The DAI hosts a variety of collections which together reinforce the importance, past and present, of Newfoundland and Labrador's history and culture. I will give an oral presentation of the project followed by a demonstration of the Otto Kelland Dories exhibit on the Digital Archives Initiative (DAI) at Memorial University of Newfoundland. I will be happy to answer questions following my presentation.

Comparative evaluation of the proximate composition of smoked and salted-dried Oreochromis niloticus

This study was carried out to evaluate and compare the shelf life of smoked and salted-dried Oreochromis niloticus over a relative time period. Improved traditional smoking kiln and salting were employed respectively. The smoking kiln was constructed with iron metal with a dimension of 120cm x 70cm and consisting of three smoking racks with dimension of 30 x 30cm each. Table salt was used for preservation of some of the specimens. A total of 30 samples weighing 7.1kg were used. Fifteen (15) samples each were used respectively for smoking and salting. Satisfactory smoking was achieved in two days while salting to dryness was accomplished in four days. The initial percentage proximate compositions of the smoked products were 7.94%, 66. 97%, 8.`84% and 2.96% for moisture, protein, lipid and ash respectively, while that of the salted products were 8.37%, 63.93%, 12.91% and 3.95% for moisture, protein, lipid and ash. Preliminary results of the proximate compositions of the two products at the end of the fifth week of storage were as follows; 8.23%, 65.70%, 10.63% and 2.23% for moisture, protein, lipid and ash respectively of the smoked products, while 6.33%, 64.25%, 11.28% and 2.38% represent the values of moisture, protein, lipid and ash of the salted-dried products. By the individual product proximate characterization, it was discovered that both products were still relatively in good and acceptable condition. However, the protein and moisture values of the smoked products were relatively greater than those of the salted-dried products, while on the other hand, lipid and ash were relatively greater in salted-dried products. The prevailed relative higher moisture in the smoked products constitutes a predisposing condition for microbial activity and spoilage of the products, while the relative higher percentage lipid in the salted-dried products predisposes the products to lipid oxidation and rancidity.

Historical Catches of Humpback Whales, Megaptera novaeangliae, in the North Atlantic Ocean: Estimates of Landings and Removals

Whaling for humpback whales, Megaptera novaeangliae, in the North At- lantic Ocean has occurred in various forms (e.g. for local subsistence, for oil to be sold commercially, using hand harpoons and deck-mounted cannons, using oar-driven open boats and modern powered catcher boats) from the early 1600’s to the present. Several previous attempts to estimate the total numbers of humpback whales removed were considered close to comprehensive, but some uncertainties remained. Moreover, the statistical uncertainty was not consistently presented with the previous estimates. Therefore, we have pursued several avenues of additional data collection and conducted further analyses to close outstanding data gaps and address remaining issues. Our new estimates of landings and total removals of humpback whales from the North Atlantic are 21,476 (SE=214) and 30,842 (SE=655), respectively. These results include statistical uncertainty, reflect new data and improved analysis methods, and take account of some fisheries for which estimates had not been made previously. The new estimates are not sufficiently different from previous ones to resolve the major inconsistencies and discrepancies encountered in efforts to determine the conservation status of humpback whale populations in the North Atlantic.

Eden and District Fisheries Advisory Committee 17th October, 1978

This is the report from the Eden and District Fisheries Advisory Committee meeting, which was held on the 17th October, 1978. It covers information on fisheries income and expenditure, the study of salmon propagation in England and Wales, work on the future programme of fisheries work and keep nets. It also covers the report by the area fisheries officer on fishing activities including river conditions and fishing for salmon, sea trout, brown trout, and coarse fish, and an update on Holmwrangle hatchery. The report also looks at stocking numbers of salmon and sea trout in various rivers, predator counts for various rivers, biological work and fish disease. The Fisheries Advisory Committee was part of the Regional Water Authorities, in this case the North West Water Authority. This preceded the Environment Agency which came into existence in 1996.

In utero domoic acid toxicity: a fetal basis to adult disease in the California sea lion (Zalophus californianus)

California sea lions have been a repeated subject of investigation for early life toxicity, which has been documented to occur with increasing frequency from late February through mid-May in association with organochlorine (PCB and DDT) poisoning and infectious disease in the 1970's and domoic acid poisoning in the last decade. The mass early life mortality events result from the concentrated breeding grounds and synchronization of reproduction over a 28 day post partum estrus cycle and 11 month in utero phase. This physiological synchronization is triggered by a decreasing photoperiod of 11.48 h/day that occurs approximately 90 days after conception at the major California breeding grounds. The photoperiod trigger activates implantation of embryos to proceed with development for the next 242 days until birth. Embryonic diapause is a selectable trait thought to optimize timing for food utilization and male migratory patterns; yet from the toxicological perspective presented here also serves to synchronize developmental toxicity of pulsed environmental events such as domoic acid poisoning. Research studies in laboratory animals have defined age-dependent neurotoxic effects during development and windows of susceptibility to domoic acid exposure. This review will evaluate experimental domoic acid neurotoxicity in developing rodents and, aided by comparative allometric projections, will analyze potential prenatal toxicity and exposure susceptibility in the California sea lion. This analysis should provide a useful tool to forecast fetal toxicity and understand the impact of fetal toxicity on adult disease of the California sea lion.

Studies on water quality and fish diversity of Sadatpur lake in Ahmednagar district Maharashtra

The present study deals with the water quality and ichthyofauna of Sadatpur lake. Monthly changes in water quality parameters such as temperature, pH, dissolved oxygen (DO), total alkalinity, chlorides, sulphates, phosphates and nitrites have been studied for a period of one year beginning from January 2005 and were investigated to assess the suitability of this lake for fish and fisheries practices. All the physico-chemical parameters determined, revealed that the fluctuations in water temperature, pH, dissolved oxygen, alkalinity and nitrites, were within the desirable limits. On the other hand, chloride and sulphate were lower whereas phosphate was higher than the desirable limits. Altogether 24 fish species belonging to 17 genera and 2 orders were found to be present in the lake. Among fish species family Cyprinidae dominated the lake. The lake subsistence fishery served the need of local fishermen community.

Identification of causes of Thai pangas mortality in ponds and their control strategies

Mass mortality of Thai pangas (Pangasius hypophthalmus) is reported to be a big threat to monoculture of the species in Bangladesh. Twenty affected and twenty control Thai pangas ponds were investigated around Mymensingh district in order to identify the causes of pangas mortality. Sixty affected and sixty unaffected fish samples were examined and compared to find the fish-level variables associated with the disease. A range of haemorrhagic signs on snout, skin and fins were recorded during examination with naked eyes. Aeromonas spp. and Edwardsiella spp. were isolated from 87% and 80% of the affected fish, respectively. Even 4% of the seemingly healthy fish carried Aeromonas spp. on their skin. Among the four water quality parameters monitored, remarkably higher total ammonia (1.5 ppm) was found in water of the affected ponds compared to that of the unaffected ones (0.4 ppm). High ammonia in affected water caused by excessive organic decomposition and poor pond management might have reduced the immunity of fish, which predisposed them for bacterial invasion and consequent disease outbreak.

Notes on the induced maturation and spawning in four-month-old Penaeus monodon Fabricius by eyestalk ablation

The ablation technique consisted of making an incision across the eyeball to allow free flow of fluids while holding the prawn under water, squeezing the eyeball contents outwards, and pinching hard the eyestalk tissue. The cut area heals completely in about a week; no application of antibiotics is necessary. Spent spawners were tagged with thin brass rings (Rodriguez, 1976) around the unablated eyestalk for a separate experiment on rematuration. Two spawning yielding approximately 277,000 eggs were obtained three weeks after ablation, followed four days later by two more spawnings with 160,000 eggs; all four spawners weighed more than 100 g. With a hatching rate of 98% and 78% for the first and second batch, respectively, the spawnings produced viable nauplii. Water temperatures as low as 23 degree C due to a delayed cold spell in March depressed molting; weakened larvae had to be discharged at the mysis stage. Although ovarian development continued, no further spawnings were obtained due mainly to the onset of bacterial and fungal disease. Infection is initiated in injured portions of the exoskeleton, sometimes penetrating right through the muscles to the ovarian tissues. The non-flowthrough conditions and mussel meat feeding led to fouling of the culture water resulting in consecutive mortalities caused by disease. Female P.monodon held in maturation pens were ablated at the age of 15 months (Santiago, et al., 1976); they averaged only 16 g body weight after four months growth in ponds. In another experiment, pond-reared P.monodon females ranging from 50 to 80 g were ablated at approximately seven months (Aquacop, 1977). The present results show a minimum age of four months from postlarve that P.monodon is capable of ovarian development and spawning upon ablation. However, maturation is probably affected by size as well as age - the four-month old females weighed an average of 100 g in contrast to the smaller animals in the earlier experiments.

Cloning and expression of zebra fish ferroportin and investigating its influence on anemia

Iron is required for many microbes and pathogens for their survival and proliferation including Leishmania which cause leishmaniasis. Leishmaniasis is an increasingly serious infectious disease with a wide spectrum of clinical manifestations. These range from localized cutaneous leishmaniasis (CL) lesions to a lethal visceral form. Certain strains such as BALB/c mice fail to control L. major infection and develop progressive lesions and systemic disease. These mice are thought to be a model of non-healing forms of the human disease such as kala-azar or diffuse cutaneous leishmaniasis. Progression of disease in BALB/c mice has been associated with the anemia, in last days of their survival, the progressive anemia is considered to be one of the reasons of their death. Ferroportin (Fpn), a key regulator of iron homeostasis is a conserved membrane protein that exports iron across the duodenal enterocytes as well as macrophages and hepatocytes into the blood circulation. Fpn has also critical influence on survival and proliferation of many microorganisms whose growth is dependent upon iron, thus preparation of Fpn is needed to study the role of iron in immune responses and pathogenesis of micoorganisms. To prepare and characterize a recombinant ferroportin, total RNA was extracted from Indian zebrafish duodenum, and used to synthesize cDNA by RT-PCR. PCR product was first cloned in Topo TA vector and then subcloned into the GFP expression vector pEGFP–N1. The final resulted plasmid (pEGFP-ZFpn) was used for expression of FPN-EGFP protein in Hek 293T cells. The expression was confirmed by fluorescence microscopy and flow cytometery. Recombinant Fpn was further characterized by submission of its predicted amino acid sequences to the TMHMM V2.0 prediction server (hidden Markov model), NetOGlyc 3.1 server and NetNGlyc 3.1 server. Data emphasised that obtained Fpn from indian zebrafish contained eight transmembrane domains with N- and C-termini inside the cytoplasm and harboured 78 mucin-type glycosylated amino acid. The results indicate that the prepared and characterized recombinant Fpn protein has no membrane topology difference compared to other Fpn described by other researcher. Our next aim was to deliver recombinant plasmid (pEGFP-ZFpn) to entrocyte cells. However, naked therapeutic genes are rapidly degraded by nucleases, showing poor cellular uptake, nonspecificity to the target cells, and low transfection efficiency. The development of safe and efficient gene carriers is one of the prerequisites for the success of gene therapy. Chitosan and alginate 139 polymers were used for oral gene carrier because of their biodegradability, biocompatibility and their mucoadhesive and permeability-enhancing properties in the gut. Nanoparticles comprising Alginate/Chitosan polymers were prepared by pregel preparation method. The resulting nanoparticles had a loading efficiency of 95% and average size of 188 nm as confirmed by PCS method and SEM images had showed spherical particles. BALB/c mice were divided to three groups. The first and second group were fed with chitosan/alginate nanoparticles containing the pEGFP-ZFpn and pEGFP plasmid, respectively (30 μgr/mice) and the third group (control) didn’t get any nanoparticles. The result showed BALB/c mice infected by L.major, resulted in higher hematocryte and iron level in pEGFP-ZFpn fed mice than that in other groups. Consentration of cytokines determined by ELISA showed lower levels of IL-4 and IL-10 and higher levels of IFN-γ/IL-4 and IFN-γ/IL-10 ratios in pEGFP-ZFpn fed mice than that in other groups. Morover more limited increase of footpad thickness and significant reduction of viable parasites in lymph node was seen in pEGFP-ZFpn fed mice. The results showed the first group exhibited a highr hematocryte and iron compared to the other groups. These data strongly suggests the in vivo administration of chitosan/alginate nanoparticles containing pEGFP-ZFpn suppress Th2 response and may be used to control the leishmaniasis .