15 resultados para oligonucleotide probe

em Universidad Politécnica de Madrid


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The current I to a cylindrical Langmuir probe with a bias Φp satisfying β≡eΦp/mec2∼O(1) is discussed. The probe is considered at rest in an unmagnetized plasma composed of electrons and ions with temperatureskTe∼kTi≪mec2. For small enough radius, the probe collects the relativistic orbital-motion-limited (OML) current I OML , which is shown to be larger than the non-relativistic result; the OML current is proportional to β1/2 and β3/2 in the limits β≪1 and β≫1, respectively. Unlike the non-relativistic case, the electron density can exceed the unperturbed density value. An asymptotic theory allowed to compute the maximum radius of the probe to collect OML current, the sheath radius for probe radius well below maximum and how the ratio I/I OML drops below unity when the maximum radius is exceeded. A numerical algorithm that solves the Vlasov-Poisson system was implemented and density and potential profiles presented. The results and their implications in a possible mission to Jupiter with electrodynamic bare tethers are discussed density value. An asymptotic theory allowed to compute the maximum radius of the probe to collect OML current, the sheath radius for probe radius well below maximum and how the ratio I/IOML drops below unity when the maximum radius is exceeded. A numerical algorithm that solves the Vlasov-Poisson system was implemented and density and potential profiles presented. The results and their implications in a possible mission to Jupiter with electrodynamic bare tethers are discussed.

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An asymptotic analysis of the Langmuir-probe problem in a quiescent, fully ionized plasma in a strong magnetic field is performed, for electron cyclotron radius and Debye length much smaller than probe radius, and this not larger than either ion cyclotron radius or mean free path. It is found that the electric potential, which is not confined to a sheath, controls the diffusion far from the probe; inside the magnetic tube bounded by the probe cross section the potential overshoots to a large value before decaying to its value in the body of the plasma. The electron current is independent of the shape of the body along the field and increases with ion temperature; due to the overshoot in the potential, (1) the current at negative voltages does not vary exponentially, (2) its magnitude is strongly reduced by the field, and (3) the usual sharp knee at space potential, disappears. In the regions of the C-V diagram studied the ion current is negligible or unaffected by the field. Some numerical results are presented.The theory, which fails beyond certain positive voltage, fields useful results for weak fields, too.

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A theory is presented for a method, recently proposed by Hester and Sonin, of determining the ion temperature in a plasma by measuring the transient current to a cylindrical Langmuir probe after applying a potential Vp{ — eVpy>KTe) under conditions where collection is collision free and the ratio of probe radius to Debye length is small. The ion component of the current does not approach its final steady-state value monotonicalfy, but exhibits a strong, ion-temperature-dependent overshoot in the first few ion-plasma periods following the biasing of the probe. Analytical formulas are derived for the case of a Maxwellian plasma, and convenient graphical results are presented. The possible masking of the overshoot by a transient displacement current is discussed; it is shown how to avoid such displacement effects. For the overshoot to be sensitive to the ion temperature T the probe must be near plasma (zero) potential before applying V1,(eVp~<0.lKTe, VP~ being that initial potential); this is not a drawback of the method, but, on the contrary, it can be used to accurately determine plasma potential along with T.

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A theory is presented for an end effect in the current response of a highly negative, cylindrical Langmuir probe in a collisionless plasma flow. Under conditions where the ratio of probe radius to debye length is small and the ion-acoustic Mach number is large, the current exhibits a strong peak when the probe axis is brought into alignment with the flow direction. Closed formulas are given for the maximum and angular half-width of the peak, and universal graphical results are presented for the entire peak structure. The theory shows very good agreement with experimental data. The use of the end effect for diagnostic purposes, in particular, for the determination of the ion temperature, is discussed.

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Collisional analysis of electron collection by a probe in a strongly magnetized, fully ionized plasma is carried out. A solution to the complete set of macroscopic equations with classical transport coefficients that is wholly consistent in the domain is determined; R and le are probe radius and electron gyroradius, respectively. If R2/le 2 is large compared with mi/3me probe large compared with ion gyroradius, ion–electron energy exchange—rather than electron heat diffusion—keeps electrons isothermal. For smaller probes at negative bias, however, electron cooling occurs in the plasma beyond the sheath, with a potential overshoot lying well away from it. The probe characteristic in the electron-retarding range may then mimic the characteristic for a two electron-temperature plasma and lead to an overestimate of electron temperature; the validity of these results for other transport models is discussed

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The electron-retarding range of the current-voltage characteristic of a flat Langmuir probe perpendicular to a strong magnetic field in a fully ionized plasma is analysed allowing for anomalous (Bohm) cross-field transport and temperature changes in the collection process. With probe size and ion thermal gyroradius comparable, and smaller than the electron mean free path, there is an outer quasineutral region with ion viscosity determinant in allowing nonambipolar parallel and cross flow. A potential overshoot lying either at the base or inside the quasineutral region both makes ions follow Boltzmann's law at negative bias and extends the electron-retarding range to probe bias e(j)p ~ +2Too. Electron heating and cooling occur roughly at positive and negative bias, with a re-minimum around efa ~ - 2 7 ^ ; far from the probe heat conduction cools and heats electrons at and radially away from the probe axis, respectively. The potential overshoot with no thermal effects would reduce the electron current Ie, making the In Ie versus 4>p graph downwards-concave,but cooling further reduces Ie substantially, and may tilt the slope upwards past the temperature minimum. The domain of strict validity of our analysis is narrow in case of low ion mass (deuterium), breaking down with the ion Boltzmann law.

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Use of a conductive bare tape electrically floating in low Earth orbit as an effective electron beam source to produce artificial auroral effects, free of problems that mard tandard beams, is considered. Ambient ions impacting the tape with keV energies over most of its length liberate secondary electrons that race down the magnetic field, excite neutrals in the E layer, and result in auroral emissions. The tether would operate with both a power supply and a plasma contactor off at nighttime; power and contactor would be on at daytime for reboost. Tomographic analysis of auroral emissions from the footprint of the beam, as observed from the spacecraft, can provide density profiles of dominant neutral species in the E layer. A characteristic tether system, at altitude 300 km and moderate orbital inclination, would involve an aluminum tape with a length of 20 km, a width of 15 mm, and a thickness of 0.2 mm for a full-system mass around 1200 kg, with two thirds going into the power subsystem.

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A kinetic approach is used to develop a theory of electrostatic probes in a fully ionized plasma in the presence of a magnetic field. A consistent asymptotic expansion is obtained assuming that the electron Larmor radius is small compared to the radius of the probe. The order of magnitude of neglected terms is given. It is found that the electric potential within the tube of force defined by the cross section of the probe decays non-mono tonic ally from the probe; this bump disappears at a certain probe voltage and the theory is valid up to this voltage. The transition region, which extends beyond plasma potential, is not exponential. The possible saturation of the electron current is discussed. Restricted numerical results are given; they seem to be useful for weaker magnetic fields down to the zero-field limit. Extensions of the theory a r e considered.

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An old analysis of probe current in a strongly magnetized plasma is reconsidered. It is shown that, in the collisional limit, the plasma beyond the sheath heats up in the collection process at positive probe bias enough. The modified current is compared to the current collected in the case of collection due to Bohm diffusion.

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An electrically floating bare tether in LEO orbit may serve as upper atmospheric probe. Ambient ions bombard the negatively biased tether and liberate secondary electrons, which accelerate through the same voltage to form a magnetically guided planar e-beam resulting in auroral effects at the E-layer. This beam is free from the S/C charging and plasma interaction problems of standard e-beams. The energy flux is weak but varies accross the large beam cross section, allowing continuous observation from the S/C. A brightness scan of line-integrated emissions, that mix emitting altitudes and tether points originating the electrons, is analysed. The tether is magnetically dragged at nighttime operation, when power supply and plasma contactor at the S/C are off for electrical floating; power and contactor are on at daytime for partial current reversal, resulting in thrust. System requirements for keeping average orbital height are discussed.

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The Session Initiation Protocol (SIP) has been adopted by the IETF as the control protocol for creating, modifying and terminating multimedia sessions. Overload occurs in SIP networks when SIP servers have insufficient resources to handle received messages. Under overload, SIP networks may suffer from congestion collapse due to current ineffective SIP overload control mechanisms. This paper introduces a probe-based end-to-end overload control (PEOC) mechanism, which is deployed at the edge servers of SIP networks and is easy to implement. By probing the SIP network with SIP messages, PEOC estimates the network load and controls the traffic admitted to the network according to the estimated load. Theoretic analysis and extensive simulations verify that PEOC can keep high throughput for SIP networks even when the offered load exceeds the capacity of the network. Besides, it can respond quickly to the sudden variations of the offered load and achieve good fairness.

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Use of a (bare) conductive tape electrically floating in LEO as an effective e-beam source that produces artificial auroras, and is free of problems that have marred standard beams, is considered. Ambient ions impacting the tape with KeV energies over most of its length liberate secondary electrons, which race down the magnetic field and excite neutrals in the E-layer, resulting in auroral emissions. The tether would operate at night-time with both a power supply and a plasma contactor off; power and contactor would be on at daytime for reboost. The optimal tape thickness yielding a minimum mass for an autonomous system is determined; the alternative use of an electric thruster for day reboost, depending on mission duration, is discussed. Measurements of emission brightness from the spacecraft could allow determination of the (neutral) density vertical profile in the critical E-layer; the flux and energy in the beam, varying along the tether, allow imaging line-of-sight integrated emissions that mix effects with altitude-dependent neutral density and lead to a brightness peak in the beam footprint at the E-layer. Difficulties in tomographic inversion, to determine the density profile, result from beam broadening, due to elastic collisions, which flattens the peak, and to the highly nonlinear functional dependency of line-of-sight brightness. Some dynamical issues are discussed.

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The first feasibility study of using dual-probe heated fiber optics with distributed temperature sensing to measure soil volumetric heat capacity and soil water content is presented. Although results using different combinations of cables demonstrate feasibility, further work is needed to gain accuracy, including a model to account for the finite dimension and the thermal influence of the probes. Implementation of the dual-probe heat-pulse (DPHP) approach for measurement of volumetric heat capacity (C) and water content (θ) with distributed temperature sensing heated fiber optic (FO) systems presents an unprecedented opportunity for environmental monitoring (e.g., simultaneous measurement at thousands of points). We applied uniform heat pulses along a FO cable and monitored the thermal response at adjacent cables. We tested the DPHP method in the laboratory using multiple FO cables at a range of spacings. The amplitude and phase shift in the heat signal with distance was found to be a function of the soil volumetric heat capacity. Estimations of C at a range of moisture contents (θ = 0.09– 0.34 m3 m−3) suggest the feasibility of measurement via responsiveness to the changes in θ, although we observed error with decreasing soil water contents (up to 26% at θ = 0.09 m3 m−3). Optimization will require further models to account for the finite radius and thermal influence of the FO cables. Although the results indicate that the method shows great promise, further study is needed to quantify the effects of soil type, cable spacing, and jacket configurations on accuracy.

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This study focuses on the relationship between CO2 production and the ultimate hatchability of the incubation. A total amount of 43316 eggs of red-legged partridge (Alectoris rufa) were supervised during five actual incubations: three in 2012 and two in 2013. The CO2 concentration inside the incubator was monitored over a 20-day period, showing sigmoidal growth from ambient level (428 ppm) up to 1700 ppm in the incubation with the highest hatchability. Two sigmoid growth models (logistic and Gompertz) were used to describe the CO2 production by the eggs, with the result that the logistic model was a slightly better fit (r2=0.976 compared to r2=0.9746 for Gompertz). A coefficient of determination of 0.997 between the final CO2 estimation (ppm) using the logistic model and hatchability (%) was found.