Datos de precisión en la evaluación del ruido transmitido al interior de una habitación según el Real Decreto 1367/2007

El objetivo del presente trabajo es realizar una evaluación realista de la incertidumbre en los ensayos de ruido transmitido al interior de una sala según el Anexo IV del Real Decreto 1367/2007, contemplando todas las posibles causas que intervienen en la incertidumbre de forma que las imprecisiones observadas en los ejercicios de intercomparación se cubran con un único ensayo. Otra parte fundamental del trabajo es cuantificar la fuente de incertidumbre generada en la elección del punto de medición. En primer lugar se realiza la medición del nivel continuo equivalente ponderado A en habitaciones de distinto tamaño, a la que llegan los distintos tipos de ruido emitido desde el exterior de las mismas. El siguiente paso es realizar un análisis de los niveles medidos, tanto en su distribución espacial como en su evolución temporal, incidiendo en los valores máximos, LAeq,5s. A continuación se comprueba si la distribución de los niveles medidos se ajusta a una distribución normal mediante el software de análisis estadístico STATGRAPHICS. Determinando, en base al tamaño de las muestras escogidas, por medio de análisis estadísticos, la aportación a la incertidumbre generada en la elección del punto de medida, cuantificando su valor. Por último se realiza una medición del ruido de actividades según el Real Decreto 1367/2007, aportando una evaluación de la incertidumbre, teniendo en cuenta todas las fuentes que la generan, mediante el enfoque clásico de la GUM. ABSTRACT. The objective of this work is to carry out a realistic assessment of the uncertainty in the trials of noise transmitted into the interior of a room according to Annex IV of the Royal Decree 136772997, considering all of the causes involved in uncertainty in such a way that the inaccuracies observed in intercomparison exercises are covered with a single trial. Another fundamental part of the work is to quantify the source of uncertainty in the choice of the point of measurement. First is the weighted equivalent of the continuous level measurement in rooms of different sizes, which reach different types of noise emitted from outside of them. The next step is to perform an analysis of the measured levels, both in their spatial distribution and their temporal evolution, influencing the maximum values, LAeq,5s. Then it is checked to see whether the distribution of the measured levels conforms to a normal distribution using the STATGRAPHICS statistical analysis software. Determining therefore, based on the size of the selected samples, through statistical analysis, the contribution to the uncertainty generated in the choice of the measurement point, quantifying its value. At last is the measure of the noise of activities according to the Royal Decree 1367/2007, providing an assessment of the uncertainty, taking into account all sources that generate it, using the classical approach to the GUM.

Induction of ovulation in rabbit does using purified nerve growth factor and camel seminal plasma

The presence of an ovulation-inducing factor (OIF) in the seminal plasma (SP) of several species with spontaneous and induced ovulation, including the rabbit, has been documented. Recent studies have demonstrated that the OIF in the SP of camels (SPCAM) is a nerve growth factor (β-NGF). The aim of this study was to determine if purified β-NGF from mouse submandibular glands or SPCAM could provoke ovulation induction in the rabbit doe. A total of 35 females were synchronized with 25 IU of equine chorionic gonadotropin (Serigan, Laboratorios Ovejero, Spain) and allocated into 4 groups. Forty-eight hours later (Day 0), does were given a single dose (IM) of 1 mL of saline solution (SS; n = 8); 1 mL of gonadorelin (GnRH; Inducel, Laboratorios Ovejero, Spain; n = 9); 24 µg of β-NGF (2.5S-NGF; Promega, USA; n = 10); or 1 mL of centrifuged raw camel SP (SPCAM; 127 pg mL–1 NGF; n = 8). After treatment, an empty catheter was introduced through the vagina to simulate the nervous/mechanical stimulus of coitus (4 animals per group). Plasma LH concentrations were determined in blood samples taken 30 min before treatment and at 0, 30, 60, 90, and 120 min after injection. Progesterone concentrations were assessed at 0 and 120 min and every 2 days until Day 6 after treatment. Concentrations of β-NGF in camel SP and hormone determinations were made by enzyme immunoassay. Ovulation rate (OR) was determined after euthanasia on Day 7.