Descripción del análisis microbológico realizado mediante maldi-tof en muestras de la fundación santa fe de Bogotá.


Autoria(s): Parra Moreno, Christian René; Rojas Montenegro, José Nicolás
Contribuinte(s)

Guevara Pulido, Fredy Orlando

Data(s)

08/07/2016

Resumo

Introducción: La rápida detección e identificación bacteriana es fundamental para el manejo de los pacientes críticos que presentan una patología infecciosa, esto requiere de métodos rápidos para el inicio de un correcto tratamiento. En Colombia se usan pruebas microbiología convencional. No hay estudios de espectrofotometría de masas en análisis de muestras de pacientes críticos en Colombia. Objetivo general: Describir la experiencia del análisis microbiológico mediante la tecnología MALDI-TOF MS en muestras tomadas en la Fundación Santa Fe de Bogotá. Materiales y Métodos: Entre junio y julio de 2013, se analizaron 147 aislamientos bacterianos de muestras clínicas, las cuales fueron procesadas previamente por medio del sistema VITEK II. Los aislamientos correspondieron a 88 hemocultivos (60%), 28 urocultivos (19%), y otros cultivos 31 (21%). Resultados: Se obtuvieron 147 aislamientos con identificación adecuada a nivel de género y/o especie así: en el 88.4% (130 muestras) a nivel de género y especie, con una concordancia del 100% comparado con el sistema VITEK II. El porcentaje de identificación fue de 66% en el grupo de bacilos gram negativos no fermentadores, 96% en enterobacterias, 100% en gérmenes fastidiosos, 92% en cocos gram positivos, 100% bacilos gram negativos móviles y 100% en levaduras. No se encontró ninguna concordancia en bacilos gram positivos y gérmenes del genero Aggregatibacter. Conclusiones: El MALDI-TOF es una prueba rápida para la identificación microbiológica de género y especie que concuerda con los resultados obtenidos de manera convencional. Faltan estudios para hacer del MALDI-TOF MS la prueba oro en identificación de gérmenes.

Jorge Armando Carrizosa Gonzalez

Introduction: Rapid detection and bacterial identification is essential for the management of critically ill patients who have an infectious disease, this requires quick and accurate for the start of proper treatment methods. In Colombia conventional microbiology tests are used. No studies mass spectrometry analysis of samples in critically ill patients in Colombia. Objective: Describe the experience of microbiological analysis by MALDI-TOF MS in samples taken at the Santa Fe de Bogota Foundation technology. Materials and Methods: Between June and July 2013, 147 bacterial isolates from clinical samples, which were previously processed by the VITEK IIsystem were analyzed. 88 isolates were blood cultures (60%), 28 urine cultures (19%), and other crops 31 (21%). Results: Of the 147 isolates obtained proper identification to genus and / or species as follows: 88.4% in (130 samples) to genus and species, with a correlation of 100% compared to the VITEK II system. The identification percentage was 66% in gram negative bacilli group nonfermenting, 96% in Enterobacteriaceae, 100% in fastidious bacteria, 92% in gram-positive cocci, gram-negative bacilli 100% and 100% mobile yeast. There was no correlation in gram-positive bacilli and germs of the genus Aggregatibacter. Conclusions: The MALDI-TOF MS is a quick and easy for microbiological identification of genus and species which correlates with results obtained conventionally test. There are no studies to make the MALDI-TOF MS gold proof identification of germs.

Formato

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http://repository.urosario.edu.co/handle/10336/12277

Idioma(s)

spa

Publicador

Facultad de Medicina

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

instname:Universidad del Rosario

reponame:Repositorio Institucional EdocUR

1. Andrew M. Robinson A, James E, et al. Preparation of positive blood cultures for direct MALDI-ToF MS identification. Journal of Microbiological Methods, 28 May 2016.

2. Joanna H, Jan M, Zenon J, et al. Challenges in biomarker discovery with MALDI-TOF MS. Clinica Chimica ActaVolume 458, 1 July 2016.

3. Biswas S, Rolain J, et al. Use of MALDI-TOF mass spectrometry for identification of bacteria that are difficult to culture. Journal of Microbiological Methods. (2013) 92:14-24.

4. Riedel S, Carroll K, et al. Early Identification and Treatment of Pathogens in Sepsis: Molecular Diagnostics and Antibiotic Choice. Clin Chest Med. 2016 Jun;37(2):191-207.

5. Andrew E, Erin J, Amit A, et al. Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: A Fundamental Shift in the Routine Practice of Clinical Microbiology. Clin Microbiol Rev. 2013 Jul; 26(3): 547–603.

6. Neelja S, Manish K, Pawan K, et al. MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis. Front Microbiol. 2015; 6: 791.

7. Singer M, Deutschman C, Warren C, et al. The Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3). JAMA. 2016;315(8):801-810.

8. Buchan B, Ledeboer N. Emerging Technologies for the Clinical Microbiology Laboratory. Clin Microbiol Rev. 2014 Oct; 27(4): 783–822.

9. Stephanie S, Bereneice M, Susan R, et al. Effectiveness of Practices to Increase Timeliness of Providing Targeted Therapy for Inpatients with Bloodstream Infections: A Laboratory Medicine Best Practices Systematic Review and Meta-analysis. Clin Microbiol Rev. 2016 Jan; 29(1): 59–103.

10. Yun F, Jianfeng F. Rapid laboratory diagnosis for respiratory infectious diseases by using MALDI-TOF mass spectrometry. J Thorac Dis. 2014 May; 6(5): 507–511.

11. Yang L, Bing G, Genyan L, et al. MALDI-TOF MS versus VITEK 2 ANC card for identification of anaerobic bacteria. J Thorac Dis. 2014 May; 6(5): 517–523.

12. Paulette L, Marcela M, et al. Impacto de la espectrometría de masas por MALDI-TOF MS en la identificación rápida de bacterias aeróbicas y anaeróbicas de importancia clínica. Rev Chilena Infectol 2013; 30 (2): 140-146.

13. Andreas W, Lukas S, jette J, et al. MALDi – TOF MS in microbiological dianostics – identification of microorganisms and beyond. Appl Microbiol Biotechnol (2012) 93: 965-974.

14. Eigner U, Holfelder M, Oberdorfer K, et al. Performance of a matrix-assisted laser desorption ionization-time-of-flight mass spectrometry system for the identification of bacterial isolates in the clinical routine laboratory. Clin Lab. 2009. 55(7–8):289–296.

15. Saffert R, Cunningham S, Ihde S, et al. Comparison of Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometer to BD Phoenix automated microbiology system for identification of gram-negative bacilli. J Clin Microbiol 2011; 49: 887-92.

16. Biswas S, Rolain J, et al. Use of MALDI-TOF mass spectrometry for identification of bacteria that are difficult to culture. Journal of Microbiological Methods. (2013) 92:14-24.

17. La Scola B, Raoult D, et al. Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry. PLoS ONE 2009; 4: e8041.

18. Right J, Claydon M., Soufian M, et al. Rapid typing of bacteria using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and pattern recognition software. J. Microbiol. Methods (2002) 48, 127–138.

19. Chen Y, Liu Y, Teng S, et al. Evaluation of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Bruker Biotyper for identification of Penicillium marneffei, Paecilomyces species, Fusarium solani, Rhizopus species, and Pseudallescheria boydii.Front Microbiol. 2015 Jul 8;6:679.

20. Seng P, Drancourt M, Gouriet F, et al. Ongoing revolution in bacteriology: Routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis 2009; 49:543-51.

21. Couturier M, Mehinovic E, Croft A, et al. Identification of HACEK clinical isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2011; 49: 1104-6.

22. Bettina S, Katharina B, Guido V, et al. Identification of Gram-Positive Cocci by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Comparison of Different Preparation Methods and Implementation of a Practical Algorithm for Routine Diagnostics. J Clin Microbiol. 2013 Jun; 51(6): 1834–1840.

23. Buchan B, Ledeboer N, et al. Advances in identification of clinical yeast isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2013 May;51(5):1359-66.

24. Schmidt V, jarosch A, März P, et al. Rapid identification of bacteria in positive blood culture by matrix-assited laser desorption ionization time-of-flight mass espectrometry. Eur J Clin Microbiol Infec Dis (2012) 31: 311-317.

25. Bizzini A, Durussel C, Bille J, et al. Performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory. J Clin Microbiol 2010 48(5):1549–1554.

26. Seng P, Drancourt M, La Scola B, et al. Ongoing revolution in bacteriology: Routine identification of bacteria by matrix-asssited laser desorption ionization time-of-flight mass septrometry. Clin Infect Dis 2009; 49: 543 – 51.

27. Christner M, Rohde H, Wolters M, et al Rapid identification of bacteria from positive blood culture bottles by use of matrix-assisted laser desorption-ionization time of flight mass spectrometry fingerprint- ing. J Clin Microbiol (2010) 48(5):1584–1591.

28. A Ferroni, S Suaréz, JL Beretti, et al. Real-Time Identification of Bacteria and Candida Species in Positive Blood Cultures Broths by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. Jurnal of Clinical Microbiology. (2010) May: vol 48 Nº 5: 1542-1548.

29. Moussaoui W, Jaulhac B, Hoffmann A, et al. Matrix-assisted laser desorption ionization time- of-flight mass spectrometry identifies 90% of bacteria directly from blood culture vials. Clin Microbiol Infect (2010)16:1631–1638.

30. Prod’hom G, Bizzini A, Durussel C, et al. Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets. J Clin Microbiol 2010 48(4):1481–1483.

31. Hoffman H, Stindl S, Ludwig W, et al. Enterobacter hormaechei subsp. Oharae subsp. Nov., E. Hormaechei subsp. Hormaechei comb. Nov., and E. Hormaechei subsp. Steigerwaltii subsp. Nov., three new subspecies of clinical importance. J Clin Microbiol 2005; 43(7): 3297-303.

32. García P, Allende F, Legarrafa P, et al. Identificación bacteriana basada en el espectro de masas de proteínas: Una nueva mirada a la microbiología del siglo XXI. Rev Chilena Infectol 2012; 29 (3): 263-272.

33. Doern G, Vautour M, Gaudet B, et al. Clinical impact of rapid in vitro susceptibility testing and bacterial identification. J. Clin. Mi- crobiol. 1994 32:1757–1762.

34. Harbarth S, GarbinoJ. Pugin J, et al. Inappropriate initial antimicrobial therapy and its effect on survival in a clinical trial of immunomodulating therapy for severe sepsis. Am. J. Med. 2003 115:529–535.

35. Jeong S, Hong J, Kim JO, et al. Identification of Acinetobacter Species Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.Ann Lab Med. 2016 Jul;36(4):325-34.

36. Ibrahim E, Sherman S, Ward V, et al. The influence of inadequate antimicrobial treatment of bloodstream infec- tions on patient outcomes in the ICU setting. Chest 2000 118:146–155.

37. Mellmann A, Cloud J, Maier T, et al. Evaluation of matrix-assisted laser desorption ionizationtime-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of non-fermenting bacteria. J Clin Microbiol 2008; 46: 1946-54.

38. Bessède E, Solecki O, Sifré E, et al. Identification of Campylobacter species and related organisms by matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Clin Microbiol Infect. 2011 Nov;17(11):1735-9.

TEME

Palavras-Chave #Análisis microbiológico #Muestras clínicas #Control de infecciones #576 #Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción #MALDI – TOF MS #VITEK II #Bacterial identification
Tipo

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info:eu-repo/semantics/acceptedVersion