Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types by use of real-time PCR and high-resolution melt analysis of the MAP1506 locus
Data(s) |
01/04/2010
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Resumo |
High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on type-specific nucleotide sequences in MAP1506, a member of the PPE (proline-proline-glutamic acid) gene family. |
Formato |
application/pdf |
Identificador | |
Idioma(s) |
en |
Publicador |
American Society for Microbiology |
Relação |
http://eprints.ucm.es/39691/ http://jcm.asm.org/content/48/4/1474.full 10.1128/JCM.02484-09 AP2005-0696 GAN |
Direitos |
info:eu-repo/semantics/restrictedAccess |
Palavras-Chave | #Veterinaria |
Tipo |
info:eu-repo/semantics/article PeerReviewed |