Clinical MRSA isolates from skin and soft tissue infections show increased in vitro production of phenol soluble modulins
Data(s) |
01/10/2015
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Resumo |
BACKGROUND: Phenol-soluble modulins (PSMs) are amphipathic, pro-inflammatory proteins secreted by most Staphylococcus aureus isolates. This study tested the hypothesis that in vitro PSM production levels are associated with specific clinical phenotypes. METHODS: 177 methicillin-resistant S. aureus (MRSA) isolates from infective endocarditis (IE), skin and soft tissue infection (SSTI), and hospital-acquired/ventilator-associated pneumonia (HAP) were matched by geographic origin, then genotyped using spa-typing. In vitro PSM production was measured by high performance liquid chromatography/mass spectrometry. Statistical analysis was performed using Chi-squared or Kruskal-Wallis tests as appropriate. RESULTS: Spa type 1 was significantly more common in SSTI isolates (62.7% SSTI; 1.7% IE; 16.9% HAP; p < 0.0001) while HAP and IE isolates were more commonly spa type 2 (0% SSTI; 37.3% IE; 40.7% HAP; p < 0.0001). USA300 isolates produced the highest levels of PSMs in vitro. SSTI isolates produced significantly higher quantities of PSMα1-4, PSMβ1, and δ-toxin than other isolates (p < 0.001). These findings persisted when USA300 isolates were excluded from analysis. CONCLUSIONS: Increased in vitro production of PSMs is associated with an SSTI clinical source. This significant association persisted after exclusion of USA300 genotype isolates from analysis, suggesting that PSMs play a particularly important role in the pathogenesis of SSTI as compared to other infection types. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Elsevier |
Relação |
http://dro.deakin.edu.au/eserv/DU:30081598/athan-clinicalmrsa-2015.pdf http://www.dx.doi.org/10.1016/j.jinf.2015.06.005 |
Direitos |
2015, Elsevier |
Palavras-Chave | #Endocarditis #MRSA #Phenol soluble modulin #Pneumonia #Skin and soft tissue infection #Science & Technology #Life Sciences & Biomedicine #Infectious Diseases #Skin and soft infection #RESISTANT STAPHYLOCOCCUS-AUREUS #COAGULASE-NEGATIVE STAPHYLOCOCCI #GENE REGULATOR AGR #UNITED-STATES #VIRULENCE DETERMINANTS #COMPLICATED SKIN #PCR ASSAY #METHICILLIN #VANCOMYCIN |
Tipo |
Journal Article |