Multiplexed detection: fast comprehensive sample analysis of tobacco leaf extracts using HPLC with AFT columns
Data(s) |
01/01/2015
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Resumo |
Detector-based comprehensive screening analysis of complex samples of natural origin using High Performance Liquid Chromatography (HPLC) can be a complicated and time-consuming task. There are a number of ways multidetection characterization can be achieved; however, there are limitations associated with each technique. Active Flow Technology (AFT) in Parallel Segmented Flow (PSF) mode allows for multiplexed detection HPLC analysis within a single injection, whereas maintaining chromatographic performance and allowing the use of multiple destructive detectors to achieve a comprehensive yet efficient screening of a complex sample. In this study, a comprehensive characterization analysis of tobacco leaf extract was carried out through multiplexed detection using a PSF column for the detection of biomolecules by UV-Vis detection, DPPH• for reactive-oxygen species (ROS) detection, and mass spectrometry, the latter two detection methods being sample destructive. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Taylor & Francis |
Relação |
http://dro.deakin.edu.au/eserv/DU:30080540/pravadali-cekicmultiplexed-2015.pdf http://www.dx.doi.org/10.1080/10826076.2015.1105256 |
Direitos |
2015, Taylor & Francis |
Palavras-Chave | #Active flow technology #DPPH center dot #High performance liquid chromatography #Multiplexed detection #Natural products #Parallel segmented flow |
Tipo |
Journal Article |