Scaffolds for controlled release of cartilage growth factors
Contribuinte(s) |
Micro et nanomédecines biomimétiques (MINT) ; Université d'Angers (UA) - Institut National de la Santé et de la Recherche Médicale (INSERM) |
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Data(s) |
2015
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Resumo |
International audience <p>In recent years, cell-based therapies using adult stem cells have attracted considerable interest in regenerative medicine. A tissue-engineered construct for cartilage repair should provide a support for the cell and allow sustained in situ delivery of bioactive factors capable of inducing cell differentiation into chondrocytes. Pharmacologically active microcarriers (PAMs), made of biodegradable and biocompatible poly (d,l-lactide-co-glycolide acid) (PLGA), are a unique system which combines these properties in an adaptable and simple microdevice. This device relies on nanoprecipitation of proteins encapsulated in polymeric microspheres with a solid in oil in water emulsion-solvent evaporation process, and their subsequent coating with extracellular matrix protein molecules. Here, we describe their preparation process, and some of their characterization methods for an application in cartilage tissue engineering.</p> |
Identificador |
hal-01392462 https://hal.archives-ouvertes.fr/hal-01392462 DOI : 10.1007/978-1-4939-2938-2_12 OKINA : ua14680 |
Idioma(s) |
en |
Publicador |
HAL CCSD Humana Press (Springer Imprint) |
Relação |
info:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-4939-2938-2_12 |
Fonte |
ISSN: 1064-3745 Methods in Molecular Biology -Clifton then Totowa- https://hal.archives-ouvertes.fr/hal-01392462 Methods in Molecular Biology -Clifton then Totowa-, Humana Press (Springer Imprint), 2015, 1340, pp.171-180. <http://link.springer.com/protocol/10.1007/978-1-4939-2938-2_12>. <10.1007/978-1-4939-2938-2_12> http://link.springer.com/protocol/10.1007/978-1-4939-2938-2_12 |
Palavras-Chave | #Chondrogenic differentiation #Mesenchymal Stem Cells #Pharmacologically active microcarriers #Protein encapsulation #Transforming growth factor #[SDV] Life Sciences [q-bio] |
Tipo |
info:eu-repo/semantics/article Journal articles |