Isolation and Validation of Anti-B7-H4 scFvs from an Ovarian Cancer scFv Yeast-Display Library.
Data(s) |
2015
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Resumo |
B7-H4 (VTCN1, B7x, B7s) is an inhibitory modulator of T-cell response implicated in antigen tolerization. As such, B7-H4 is an immune checkpoint of potential therapeutic interest. To generate anti-B7-H4 targeting reagents, we isolated antibodies by differential cell screening of a yeast-display library of recombinant antibodies (scFvs) derived from ovarian cancer patients and we screened for functional scFvs capable to interfere with B7-H4-mediated inhibition of antitumor responses. We found one antibody binding to B7-H4 that could restore antitumor T cell responses. This chapter gives an overview of the methods we developed to isolate a functional anti-B7-H4 antibody fragment. |
Identificador |
https://serval.unil.ch/?id=serval:BIB_002FBB72C992 isbn:1940-6029 (Electronic) pmid:26060068 doi:10.1007/978-1-4939-2748-7_2 |
Idioma(s) |
en |
Fonte |
Methods In Molecular Biology, vol. 1319, pp. 37-49 |
Palavras-Chave | #Animals; Antigen Presentation; B-Lymphocytes/immunology; Female; Gene Library; Humans; Mice; Ovarian Neoplasms/blood; Ovarian Neoplasms/immunology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Single-Chain Antibodies/genetics; Single-Chain Antibodies/isolation & purification; T-Lymphocytes/immunology; V-Set Domain-Containing T-Cell Activation Inhibitor 1/metabolism; Xenograft Model Antitumor Assays |
Tipo |
info:eu-repo/semantics/article article |